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Albumin indocyanine green paclitaxel compound as well as preparation method and application thereof

A technology of paclitaxel complex and indocyanine green, which is applied in the field of albumin indocyanine green paclitaxel complex and its preparation, can solve the problem of low tumor accumulation, poor blood circulation time, ICG and albumin Combined with poor stability and other issues, it can achieve good water solubility and biocompatibility, improve blood circulation time, and increase the effect of enrichment

Active Publication Date: 2014-09-17
SUZHOU INNOVATIVE BIOMATERIALS & PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the binding stability of ICG and albumin is not very good, and the blood circulation time is not very good, and the enrichment at the tumor site is not high

Method used

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  • Albumin indocyanine green paclitaxel compound as well as preparation method and application thereof
  • Albumin indocyanine green paclitaxel compound as well as preparation method and application thereof
  • Albumin indocyanine green paclitaxel compound as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1: Preparation of albumin indocyanine green paclitaxel complex:

[0056] With albumin (HSA) as the substrate, indocyanine green and paclitaxel are adsorbed by hydrophobic interaction, and paclitaxel can be used as a cross-linking agent to make albumin self-assemble into large nanoparticles, forming albumin indocyanine green paclitaxel complex (HSA- ICG-PTX). The specific preparation method is as follows:

[0057] Dissolve indocyanine green (ICG) in dimethyl sulfoxide solution to make a 10 mg / mL indocyanine green solution, and dissolve paclitaxel (PTX) in ethanol to make a 10 mg / mL paclitaxel solution; Take 2 mg of human serum albumin (HSA), dissolve it in 2 mL of phosphate buffered saline solution, and prepare a 1 mg / mL phosphate buffered saline solution of human serum albumin;

[0058] Indocyanine green solution and paclitaxel solution are added to the phosphate buffer solution of human serum albumin in different proportions to obtain albumin indocyanine w...

Embodiment 2

[0060] Embodiment 2, the preparation of albumin indocyanine green complex

[0061] Dissolve indocyanine green (ICG) in dimethyl sulfoxide solution to form a 10 mg / mL indocyanine green solution; weigh 2 mg of human serum albumin (HSA), dissolve it in 2 mL of phosphate buffered saline, Then add 6 μL of indocyanine green solution, and stir overnight (12h) in the dark; finally, the mixture obtained after stirring overnight is centrifuged at 14800rpm for 5min to remove unreacted ICG, and the supernatant is albumin-indocyanine green complex ( HSA-ICG).

Embodiment 3

[0062] Embodiment 3, preparation of albumin-paclitaxel complex

[0063] Adding the paclitaxel solution in different proportions to the phosphate buffer solution of human serum albumin can obtain the albumin-paclitaxel complexes in different proportions.

[0064] Among them, paclitaxel (PTX) was dissolved in ethanol to prepare a 10 mg / mL paclitaxel solution; 2 mg of human serum albumin (HSA) was weighed, dissolved in 2 mL of phosphate buffered saline solution, and then 25 μL of paclitaxel solution was added and stirred Overnight (12h); Finally, the mixture obtained after stirring overnight was centrifuged at 14800rpm for 5min to remove unreacted PTX, and the supernatant was 0.2mg / ml nab-paclitaxel complex (HSA-PTX).

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Abstract

The invention relates to the field of biologic medicines, and discloses an albumin indocyanine green paclitaxel compound as well as a preparation method and an application thereof. The albumin indocyanine green paclitaxel compound consists of indocyanine green, paclitaxel and human serum albumin, wherein the indocyanine green and the paclitaxel are absorbed onto human serum albumin through a hydrophobic force. The compound is good in water solubility, biocompatibility and dispersity, can generate heat in laser radiation, can promote more material cells, can kill tumor cells, is further high in fluorescence property, and can instruct joint treatment of photo-thermal therapy and chemotherapy in a fluorescence imaging mode. The preparation of the albumin indocyanine green paclitaxel compound is simple to operate and can be applied to on-scale preparation of the albumin indocyanine green paclitaxel compound, and the raw materials are easily available.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an albumin indocyanine green paclitaxel complex and a preparation method and application thereof. Background technique [0002] Cancer is one of the major malignant diseases that threaten human health. Although a large amount of human, material and financial resources have been invested in the prevention and treatment of cancer for decades since the 1950s, the progress made by human beings in this regard is very limited. [0003] Paclitaxel is a widely used anticancer drug after doxorubicin and cisplatin. It is mainly suitable for ovarian cancer and breast cancer, and it is also effective for lung cancer, colorectal cancer, melanoma, head and neck cancer, lymphoma, and brain tumor. All showed positive clinical efficacy. However, because paclitaxel is highly insoluble in water, polyoxyethylene castor oil and absolute ethanol are needed as co-solvents, and polyoxyethylene castor oil wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K41/00A61K31/337A61K47/42A61P35/00
CPCA61K31/337A61K41/00A61K47/42A61K49/00
Inventor 刘庄陈倩
Owner SUZHOU INNOVATIVE BIOMATERIALS & PHARM CO LTD
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