111results about How to "Increase the amount of enrichment" patented technology

The invention discloses a method for repairing soils polluted by heavy metal Cd, characterized in that a plant used for repairing the metal Cd is Cd-tolerant tobacco K326, a used soil chelation inducer is a hydrochloric acid solution of chitosan, microbes used for activating the heavy metal Cd in the soils are bacterial strain obtained by gathering, screening and separation in a laboratory, the three substances are combined to repair the soils polluted by the heavy metal Cd. The method comprises the following steps: cultivating a K326 seedling to the 3-leaf one-heart stage, then transplanting; before transplanting, adding chitosan; before transplanting, carrying out mass fermental cultivation of separated, screened and purified bacterial strain, adding the active bacterial strain of the heavy metal Cd at the logarithmic phase in the soils, and stirring for the subsequent use; and after transplanting the tobacco plant, regularly carrying out management. According to the invention, a plant-microorganism united repair method is adopted, and a certain amount of microbes of the active heavy metal are added in the soils to effect together, thus the repair efficiency is raised, and the efficiency of repairing the soils polluted by the heavy metal Cd is raised.

The invention discloses a preparation method of an electrode based on a gold nanoparticle doped covalent organic framework composite material and relates to the technical field of electrochemical sensors. The preparation method comprises the following steps: firstly, dissolving 1,3,5-tri(4-aminophenyl benzene) and 2,5-dimethoxy p-phthalaldehyde into a solvent, reacting under the catalysis of acetic acid and obtaining TAPB-DMTP-COF; secondly, dispersing the TAPB-DMTP-COF into methanol, and then adding chloroauric acid and a sodium borohydride methanol solution in sequence for reacting to obtainAu@ TAPB-DMTP-COF; dispersing the Au@TAPB-DMTP-COF in water to obtain suspension, and then modifying the suspension on the surface of a glassy carbon electrode, thus obtaining the modified glassy carbon electrode based on a covalent organic framework material. The invention constructs an electrochemical sensor for detecting chlorogenic acid; current response of chlorogenic acid is improved by modifying the glassy carbon electrode with the Au@TAPB-DMTP-COF.

The invention belongs to the technical field of rice deep processing, in particular to a production method of a functional rice product which has a special sweet flavor and is rich in Gamma-aminobutyric acid and a product thereof, the rice product is produced by virtue of the activities of high glutamate decarboxylase, amylase and protease in germinated brown rice of an indica type rice or a non glutinous rice. The invention takes the indica type rice or the non glutinous brown rice as a raw material which carries out the steps of cleaning, disinfection, soaking, germination, cleaning, beating into rice milk, heat insulation, transformation, drum drying or spray drying, and the like, to be produced into functional nutrition rice flake or rice powder which is rich in Gamma-aminobutyric acid. Compared with the prior art, the rice product makes full use of the activities of the glutamate decarboxylase, the amylase and the protease in the germinated brown rice; the produced rice product has the special sweet flavor; by calculating based on dry basis, the content of Gamma-aminobutyric acid is high (1,000-1,400mg / 100g), and the nutritional value and the digestibility are high. The rice product of the invention can be taken as the functional food for resisting fatigue and reducing blood lipid.

The invention provides a method for animal and plant-biochar synergistically restoring of petroleum-heavy metal polluted soil. Biological carbon base fertilizer is applied into the petroleum-heavy metal polluted soil and mixed evenly, and seasoning is conducted; earthworms are placed in the petroleum-heavy metal polluted soil to be bred after the seasoning is completed, then castor-oil plants are planted, and the castor-oil plants are removed overall after the harvesting of fruits of the castor-oil plants is completed; after the castor-oil plants are removed overall, the biological carbon base fertilizer is applied into the petroleum-heavy metal polluted soil again and mixed evenly, and seasoning is conducted; after the seasoning, earthworms are placed in the petroleum-heavy metal polluted soil to be bred, and then willows are planted; and the steps are executed circularly and repeatedly until the content of heavy metal in the soil is up to the safety standard. According to the method, the soil remediation effect is good; the combination of the biochar, animals and plants reinforces compound restoration and the effect is very obvious. In addition, secondary pollution to the soil is avoided.

The invention discloses a method for improving the repairing efficiency of heavy metal polluted plants. The method comprises the following steps: growing plants with an enriching or super-enriching ability to heavy metal on heavy metal polluted soil or water; when the main stems of the plants grow leaves, spraying a heavy metal pollution repairing improver on the leaves; removing heavy metal pollutions by harvesting dead leaves of the plants in plant growing season. The method has the advantages that not only can the repairing efficiency of the heavy metal polluted plants be improved, but also heavy metal poisoning the plants can be reduced or removed, and the application range of heavy metal polluted plant repairing is extended.

The invention relates to the field of biologic medicines, and discloses an albumin indocyanine green paclitaxel compound as well as a preparation method and an application thereof. The albumin indocyanine green paclitaxel compound consists of indocyanine green, paclitaxel and human serum albumin, wherein the indocyanine green and the paclitaxel are absorbed onto human serum albumin through a hydrophobic force. The compound is good in water solubility, biocompatibility and dispersity, can generate heat in laser radiation, can promote more material cells, can kill tumor cells, is further high in fluorescence property, and can instruct joint treatment of photo-thermal therapy and chemotherapy in a fluorescence imaging mode. The preparation of the albumin indocyanine green paclitaxel compound is simple to operate and can be applied to on-scale preparation of the albumin indocyanine green paclitaxel compound, and the raw materials are easily available.

The invention relates to a method for remediation of contaminated soil, in particular to a method for combined remediation of DDTs-PAHs combined contaminated soil. Biosurfactant rhamnolipid is combined with efficient mixed degrading bacteria to strengthen and promote organic chloride pesticide DDTs and PAHs left in facility agriculture soil for decomposition and conversion; and meanwhile low-accumulation vegetable crops are planted, and microbial growth and degradation of the organic contaminant DDTs and PAHs are further strengthened through the plant root system metabolism and the chemical stimulation function of root exudates. The method has the beneficial effects that damage to the environment is small, no secondary contamination is generated, cost is low, large-scale operation is easy, and the production and economic income are not influenced; the method is a safe and effective in-situ remediation technology capable of achieving remediation and production at the same time; and the method is suitable for remediation of the intermediate and low contamination facility agriculture soil where crops are still planted.

The invention relates to a soil remediation method for treating heavy metal pollutants. The soil is divided into three classes according to the heavy metal content in the soil, namely, heavily contaminated soil, moderately contaminated soil and lightly contaminated soil. The soil remediation method for the heavily contaminated soil comprises the steps of chemical leaching, earthworm enrichment remediation and phytoremediation; the soil remediation method for the moderately contaminated soil comprises the steps of earthworm enrichment remediation and phytoremediation; and the soil remediation method for the lightly contaminated soil is phytoremediation. The invention combines the animal / plant enhanced composite remediation, realizes in-situ bioremediation of the soil successfully and has obvious effect without causing secondary pollution to the soil.

The invention discloses a method for preventing enrichment of heavy metal in compost by combining modified nano carbon, turfgrass and a chelating agent. According to the method, 63 g of sand is put in PVC (polyvinyl chloride) pipes with heights of 25 cm and diameters of 3 cm, the height of sand is 5 cm, then, a barrier material is laid above the sand, the height of the barrier material is 5 cm, finally, 70 g of compost is put on the compost barrier material, and the height of the compost is 8 cm; and 0.3 g of festuca arundinacea seeds are sowed on the surfaces of the upper layers of the PVC pipes, and all the PVC pipes are irrigated by tap water, so that the water content of the compost and a compost mixture is kept to be 60% of the field capacity, the indoor temperature is 16 DEG C-22 DEG C, and the relative humidity is 36%-57%. The invention further discloses an application of the method for preventing enrichment of the heavy metal in the compost by combining the modified nano carbon, the turfgrass and the chelating agent in the aspect of enhancing prevention of leaching migration of the heavy metal.

The invention provides a restoration method for heavy metal polluted soil of ore districts. Castor-oil plants are planted, biological carbon base fertilizer is applied into the heavy metal polluted soil of the ore districts at the same time, the biological carbon base fertilizer is mixed evenly and seasoning is conducted; biological carbon base fertilizer is applied into the heavy metal polluted soil again after the seasoning, and seasoning is conducted again; earthworms are placed in the heavy metal polluted soil to be bred after the seasoning; after the earthworms are introduced to the soil normally, willows are planted and biological carbon base fertilizer is applied into the soil at the same time, and the willows are removed overall after a certain period; and the steps are executed circularly and repeatedly until the content of heavy metal in the soil is up to the safety standard. According to the restoration method for the heavy metal polluted soil of the ore districts, the restoration effect for the heavy metal soil of the ore districts is obvious; the restoration period is short, and secondary pollution to the soil is avoided.

The invention provides a method for restoring sludge land heavy metal contaminated soil. The method comprises the following steps: applying charcoal base fertilizer in the soil, uniformly mixing the soil with the charcoal base fertilizer, carrying out ageing, after the ageing, putting earthworms in the soil for cultivation, after introducing the earthworms in the soil normally, planting castor-oil plant and applying the charcoal base fertilizer in the soil simultaneously, after a certain period of time, removing the castor-oil plant overall, putting earthworms in the soil for cultivation, after introducing the earthworms in the soil normally, planting willows and applying the charcoal base fertilizer in the soil simultaneously, after a certain period of time, removing the willows overall, and repeating the steps in a circulating manner until content of heavy metal in the soil reaching the safe standard. With adoption of the method, the restoring treatment effect to the sludge land heavy metal contaminated soil is ideal, the treatment time is short, the territory applicability is wide, and secondary pollution to the soil can be avoided.

The invention discloses a method for using chlorpyrifos and a CNT (carbon nanometer tube) interlayer to regulate lawn grass heavy metal accumulation. The method for using the chlorpyrifos and the CNT interlayer to regulate the lawn grass heavy metal accumulation uses an oxidative modification CNT as the interlayer, aims at using the interlayer to reduce underground leakage influences of heavy metal after the chlorpyrifos and compost compound heavy metal are coupled, and provides important technical support to the oxidative modification CNT in remediation of heavy metal coupling pollution. An experiment result proves that under action of the chlorpyrifos, adding of the oxidative modification CNT mixed with all layers increases heavy metal accumulation amount of aboveground parts of festuca arundinacea, and compared with contrast groups, heavy metal total amount of percolation liquid in the CNT interlayer is reduced. The adding of the oxidative modification CNT reduces the risk that the heavy metal migrates towards underground water to a certain extent, and then technical support is provided to practical application of the method for using the chlorpyrifos and the CNT interlayer to regulate the lawn grass heavy metal accumulation.

The invention discloses a method for regulating turfgrass heavy metal accumulation by using an OTC (oxytetracycline) and carbon nano-tube interlayer. An oxide-modified carbon nano-tube is used as an interlayer. The objectives are to reduce effect of leaking heavy metal to underground after the OTC is coupled with compost compound heavy metal, and to provide important technical support for a modified carbon nano-tube repairing heavy metal coupled pollution. Experiment results show that under the action of the OTC, adding of the carbon nano-tubes mixed in layers increases heavy metal accumulation amount of the overground part of festuca arundinacea, and heavy metal total amount in percolate in the modified carbon nano-tube interlayer is lower than that of control groups. The addition of the modified carbon nano-tube reduces risks that heavy metal is transferred to underground water to a certain degree, and provides technical support for practical application of the method.

The invention discloses a hydrophobic functional micromolecule-hydrophilic polyamino acid based biodegradable polymer and a preparation method and application thereof. The preparation method includes: preparing a hydrophobic functional initiator at first, initiating an NCA monomer to realize ring-opening polymerization to obtain an amphiphilic surfactant based on functional molecules and polypeptide, and then utilizing the amphiphilic surfactant to emulsification of poly(lactic-co-glycolic acid) (PLGA), polylactic acid (PLA) and poly-Epsilon-caprolactone(PLC) materials to obtain polymer nanoparticles. Further, the emulsifier can also be used as a component constructed on surfaces of nanoparticles, the active terminal amino can be subjected to chemical bonding with targeting molecule with carboxyl to further improve stability of the nanoparticles, and meanwhile, the targeting molecule is introduced. The obtained drug-loaded targeting nanoparticles are of high stability and can be enriched to tumor location well, have good treatment efficacy to various solid tumors including human breast cancer and have low toxic and side effect.

The invention discloses a polymer, which has a narrow molecular weight distribution, which is less than 1.3, and has a general formula of B-z-H, P-B-H and P-H-B. In the general formula, B represents a block for bonding a drug, H represents a hydrophilic block, P represents a PEG block, and z represents having or not having environment-responsive chemical bond connection. The polymer forms a micelle with small particle size in water, the micelle can spontaneously aggregate to form an aggregation having a large particle size, and the aggregation has superior tumor specific enrichment than an ordinary drug carrier. The polymer has long body circulation time, and has the feature of being enriched on a tumor. The polymer can carry a drug through a covalent bond and / or a non-covalent bond, and the obtained product can further couple to a targeting molecule and / or a labelled molecule for preparing an administration system or a detection reagent.

The invention discloses a method for controlling accumulation of heavy metal in turfgrass by means of siduron and a carbon nano tube interlayer. According to the method, an oxidization modified carbon nano tube is taken as the interlayer to reduce underground seeping of heavy metal after siduron and compost compound heavy metal are coupled and provide important technical support for dealing with heavy metal coupling pollution by means of the modified carbon nano tube. Experimental results prove that under the action of siduron, the modified carbon nano tube mixed interlayer is added to increase the amount of heavy metal in the overground part of festuca arundinacea, and the total amount of percolate heavy metal in the modified carbon nano tube interlayer is reduced compared with a matched group. Due to the adding of the modified carbon nano tube, the risk that heavy metal immigrates into underground water is reduced to a certain degree, and technical support is provided for actual application of the method.

The invention provides a method for recovering acid-heavy metal contaminated soil. The method includes the steps that biological activated carbon base fertilizer is applied to the acid-heavy metal contaminated soil, mixed evenly and aged; after aging is finished, earthworms are placed in the soil to be cultured, and castor oil plants are planted and completely removed after fruits of the castor oil plants are harvested; after the castor oil plants are completely removed, earthworms are placed in the soil to be cultured again; after the earthworms are introduced into the soil normally, willows are planted, and the willows are completely removed after a period of time; the steps mentioned above are cyclically and repeatedly executed till the content of heavy metal in the soil reaches the safety standard. The method for recovering the acid-heavy metal contaminated soil has the extremely remarkable beneficial effects that a restoration period is short and the soil cannot be secondarily contaminated.

The invention discloses an extracting technology of low phenanthrene anthracene oil from coal-tar oil distillate, which comprises the following steps: allocating coal-tar anthracene oil A and high content dimethylbenzene dissolvent B into miscible liquids proportionally in crystallizing container; extracting the low phenanthrene anthracene oil through stockpile crystallization, cold washing, hot washing, melting sequentially. In comparison with the traditional technology, the invention is characterized by the following: lowing content of the phenanthrene to improve the introduction quality; reducing cooling load capacity of the crystallizing container and saving time by using high low tow-phase diaphoresis action; reducing the damage of anthracene and carbazoles; enhancing enriched quantity of the anthracene and the carbazoles; dislodging the phenanthrene in the crystallization by increasing balance time of cold washing and temperature control; dissolving easier through increasing four scouring procedures step by step to dislodge the phenanthrene in the crystallization to high degree and save the total needing time.

Belonging to the fields of functional material loaded drugs and gene technologies, the invention discloses a composite material of a functional mesoporous silica loaded drug and siRNA, a preparation method and application thereof in preparation of anticancer drugs. The composite material is prepared by the method comprising the steps of: adding mesoporous silica into a 3-mercaptopropyl trimethoxysilane solution, conducting heating reaction and separation to obtain thiolated mesoporous silica, dispersing the thiolated mesoporous silica into a 2, 2-dithiodipyridine solution, and carrying out heating reaction to obtain disulfide bond modified mesoporous silica, adding the disulfide bond modified mesoporous silica into a doxorubicin water solution, then adding thiolated siRNA, and conducting separation, thus obtaining the composite material of the functional mesoporous silica loaded drug and siRNA. The composite material provided by the invention can be applied to preparation of anticancer drugs, can load drugs into cells, significantly improves the enrichment amount of drugs in cells, plays a good mesopore blocking role, and reaches the effect of silencing gene when released in cells.

The invention discloses a preparation method of a nano particle based on polyamino acid. The preparation method of the nano particle based on the polyamino acid comprises the following steps of firstly adopting a hydrophobic hydroxy compound and p-nitro benzyl chloride acid ester as raw materials, and reacting to obtain a functional hydrophobic micromolecule activated by the p-nitro benzyl chloride acid ester; then adopting the functional hydrophobic micromolecule activated by the p-nitro benzyl chloride acid ester and a diamine compound as reactants, and reacting to preparing a hydrophobic amido compound; adopting the hydrophobic amido compound as an initiator, carrying out ring opening polymerization on alpha-amino acid-N-thiocarboxy anhydride compound to obtain a polymer based on the polyamino acid; finally dissolving the polymer based on the polyamino acid in water, then adding an acetone solution of the polymer, and stirring to obtain the functional biodegradable nano particle based on the polyamino acid. The obtained drug-loading targeting nano particle has higher stability, can be combined with targeting molecules so as to well gather to a tumor location, and has good therapeutical effect and low toxic and side effect on various solid tumors including human breast cancer.

The embodiment of the invention discloses a method for collecting and identifying trichoderma fungal volatiles and relates to the technical field of detection of trichoderma fungal volatiles. The method disclosed by the invention comprises the following steps: enriching the trichoderma fungal volatiles by adopting improved solid phase microextraction (SPME), and detecting components of the trichoderma fungal volatiles by adopting gas chromatography-mass spectrometry (GC-MS). The problem in the prior art that the components of the volatiles cannot be detected or the detected components of the volatiles are less in types because the trichoderma fungal volatiles are small in amount and difficult to collect can be solved. Real chemical information of the trichoderma fungal volatiles can be rapidly and accurately determined, comprehensive and reliable data is provided for conducting a subsequent entomological experiment, dynamic monitoring and control of soil insects can be facilitated, anda theoretical basis is provided for researching interaction between trichoderma fungi and other creatures.

The invention discloses a polyethylene glycol-modified ferrous sulfide magnetic nanometer treatment agent as well as a preparation method and application thereof, wherein the polyethylene glycol-modified ferrous sulfide magnetic nanometer treatment agent has a laminar structure, and sequentially comprises a ferrous sulfide layer, a trioctylphosphine oxide layer, an octylamine-polyacrylic acid layer, a poly allyamine hydrochloride layer and a polyacrylic acid layer from inside to outside; hexa-amino branched polyethylene glycol is modified on the polyacrylic acid layer. The treatment agent disclosed by the invention can have high enrichment amount at the tumor part by retention and osmosis action of the tumor, and then photothermal therapy is carried out, so that the effect is significant, the other parts are not damaged, and the tumor does not reappear after being cured. Compared with the prior art, the polyethylene glycol-modified ferrous sulfide magnetic nanometer treatment agent is significant in effect. In addition, the cancer can be subjected to early diagnosis by magnetic resonance imaging, meanwhile, real-time monitoring can be carried out in recovered magnetic resonance arthrography, a reasonable treatment plant can be provided by combining with a real-time monitoring result, and the polyethylene glycol-modified ferrous sulfide magnetic nanometer treatment agent can be widely applied to a clinical diagnosis and treatment technology.

The invention provides a T1-T2 double-activated magnetic resonance imaging contrast agent as well as a preparation method and application thereof. The T1-T2 double-activated magnetic resonance imagingcontrast agent has a core-shell structure and sequentially comprises T2 contrast agent nanoparticles, a silicon dioxide layer, a T1 contrast agent layer and a modification layer from inside to outside, and the T2 contrast agent nanoparticles are superparamagnetic iron oxide nanoparticles. The T1-T2 double-activated magnetic resonance imaging contrast agent is in a T1 and T2 contrast agent signaldouble-quenching state in normal tissues around an in-vivo tumor, and T1 and T2 contrast agent signals can be activated at the same time at a tumor part, so that the tumor part and the surrounding normal tissues have a remarkable MRI signal difference, and the T1-T2 double-activated magnetic resonance imaging contrast agent has targeting property on tumor cells, so as to realize high-quality imaging for the tumor part.

The invention belongs to the technical field of table vinegar production, and provides a method for co-fermenting red date and coix seed vinegar with high gamma-aminobutyric acid content through combination of monascus, lactic acid bacteria and spore bacteria. The method comprises steps as follows: mixing germinated coix seeds, coix seed bran, enzymatically decomposed red date juice and L-sodium glutamate, and carrying out alcohol fermentation by mixed bacteria of monascus with high yield of GABA, saccharomycetes and lactic acid bacteria; after the fermentation is finished, supplementing sugar, red date juice, L-sodium glutamate, lactic acid bacteria, spore bacteria and acetobacter pasteurianus, and carrying out aerobic acetic acid and lactic acid fermentation; and after the fermentation is finished, adding lactic acid bacteria for anaerobic secondary lactic acid fermentation. The red date and coix seed vinegar with high gamma-aminobutyric acid content is prepared through raw material optimization, strain optimization proportioning, multiple times of fermentation enrichment, ageing, blending, sterilization and filling. In a final product, the content of non-volatile acid is larger than or equal to 2.0 g / 100 mL, the content of flavone is larger than or equal to 120 mg / 100 mL, the content of GABA is larger than or equal to 280.0 mg / 100 mL, the content of Monacolin K is larger than or equal to 120 mg / 100 mL, and the effects of resisting oxidation, lowering cholesterol and lowering blood pressure are achieved.

The invention relates to a method for biology synergistic repairing of farmland heavy metal polluted soil and belongs to the technical field of soil repairing. The method comprises the steps that plants which can survive in the soil with high heavy metal concentration are inductively screened out at first; then a component having the capacity of chelating metal ions is extracted from traditional Chinese medicine, the complexing performance of the extract is further improved with the assistance of organic carboxylic acid in grape fermentation broth, and a metal activating agent is obtained; nutrients are provided for growth of the plants through fermentation residues and traditional Chinese medicine residues, and the heavy metal ions in the soil are made to form a soluble chelate through the synergistic effect of preferred plant exudates and the metal activating agent, so that the accumulation amount of preferred plant root systems on heavy metal is increased; and field mice are put to eat the rich root systems of the preferred plants in the soil finally, and soil heavy metal pollution repairing can be completed after the field mice are repelled through irrigation. According to the method for biology synergistic repairing of the farmland heavy metal polluted soil, the accumulation amount of the plant root systems on the heavy metal is increased through the synergistic effect of different biology; in addition, the repairing cycle is short; and the method has wide application prospects.

The invention belongs to the technical filed of biological medicine, and particularly relates to a thermo-sensitive type nano-drug preparation for in-depth delivery of tumor drugs and a preparation method and application thereof. According to the thermo-sensitive type nano-drug preparation for in-depth delivery of the tumor drugs, under the irradiation of near-infrared light (such as laser irradiation with wavelength of 650-900 nm), mild photothermal is generated, a thermo-sensitive type intermediate Azo-linker in the drug preparation is broken, oxygen independent free radicals are generated,and the effects of photothermal therapy (PTT) and photodynamic therapy (PDT) are achieved; and in addition, the enrichment of the drug preparation at the tumor vascular site can be caused by the wildphotothermal, the targeting property of the drug preparation is improved, and thus the enrichment amount of the chemotherapy drug in the tumor site is increased.

The invention relates to the technical field of bioengineering, in particular to a magnesium ion-enriched bone scaffold material and a preparation method thereof. Magnesium is enriched in decellularized biological bones, so that both natural bones and magnesium ions can play a role in bone repair. Meanwhile, natural bones have large surface areas due to highly void structures, so that magnesium ions can be easily attached to the surface layers of natural bones, osteoblasts can be better induced to adhere to a bone scaffold and grow inside the bone scaffold, and additional supplementation of magnesium in other ways is not needed.

The invention relates to a dual-response nanodrug delivery system loaded with antitumor drugs for tumor therapy and a preparation method of the nanodrug delivery system. Sodium alginate modified with11-mercaptodecanoic acid (MUA) is used as a frame, CaCO3 and antitumor drugs are coprecipitated on the frame of sodium alginate by in-situ mineralization, and through ultrasonic vibration, sulfhydrylgroups on the modified sodium alginate are promoted to be crosslinked to prepare a dual-response nanodrug delivery carrier of pH and redox. According to the present invention, in vivo and in vitro drug release, cytotoxicity experiments and animal experiments prove that nanoparticles based on CaCO3 have good tumor selectivity and the ability of regulating pH in tumor cells, and can kill and wound tumor cells while reducing toxic and side effects on normal cells and organisms. The nanodrug delivery carrier provided by the invention is expected to be used for tumor therapy, and has a good application prospect in the field of biomedicine.

The invention provides a sulfydryl-containing zwitterionic polypeptide modified doxorubicin derivative, a nano-micelle and a preparation method of the nano-micelle, and belongs to the field of biological medicines. The doxorubicin derivative has a structure as shown in a general formula (I). The preparation method of the doxorubicin derivative comprises the following steps of in a polar solvent, mixing sulfydryl-containing zwitterionic polypeptide with acryloylhydrazine to react, and performing crystallizing to obtain a polypeptide derivative; and dissolving the polypeptide derivative and doxorubicin hydrochloride in the polar solvent according to a molar ratio of 1: (0.2-5), adding a trifluoroacetic acid catalyst, and carrying out a stirring reaction to obtain the doxorubicin derivative.The nano-micelle prepared from the doxorubicin derivative is long in in-vivo blood circulation time, high in drug loading capacity, small in toxic and side effects, good in pH value response and goodin tumor inhibition effect.