The invention relates to the technical field of agricultural planting, in particular to self-made rooting liquid for planting of stevia rebaudiana and a preparation method. The rooting liquid comprises the following components of willow shoots, poplar shoots, cactus, Chinese rose branches, herbs of knotroot bristlegrass, dracocephalum moldavicum, white sugar and rice vinegar. The preparation method comprises the following steps: separately weighing the willow shoots, the poplar shoots, the cactus, the Chinese rose branches, the herbs of knotroot bristlegrass and the dracocephalum moldavicum, placing the weighed willow shoots, the weighed poplar shoots, the weighed cactus, the weighed Chinese rose branches, the weighed herbs of knotroot bristlegrass and the weighed dracocephalum moldavicum in a container for mashing, and adding water which is 5-10 times of the weighed components for soaking to obtain a mixture; filtering the mixture to obtain soaking liquid; weighing the white sugar and the rice vinegar in a compounding ratio, adding the weighed white sugar and the weighed rice vinegar to the soaking liquid for uniform mixing, and performing concentration so as to obtain the rooting liquid. According to the rooting liquid for planting of the stevia rebaudiana, and the preparation method, disclosed by the invention, the rooting liquid is good in rooting effect, low in cost, natural and free from pollution; the preparation method is simple and convenient to operate, and suitable for vast peasants to use, and has high application value.