Excavation method for salt-tolerant gene of salix matsudana

Abstract

The invention provides an excavation method for a salt-tolerant gene of salix matsudana. The method comprises the steps that salt-tolerant salix matsudana and salt-sensitive salix matsudana are crossbred to obtain an F1 population, the F1 population is divided into salt-tolerant and salt-sensitive filial generations, and through salt stress, root systems of the salt-tolerant and salt-sensitive filial generations are subjected to transcriptome sequencing to obtain differential expression gene sequences separately; based on the salt-tolerant filial generation, a high-density genetic map is established, salt tolerance QTLs positioning is conducted, a QTL section is obtained through calculation, the QTL section, the differential expression gene sequences and a salix matsudana whole genome arecompared, and through analysis, the salt-tolerant candidate gene is obtained; through salt stress, the relative expression of the salt-tolerant candidate gene of the salt-tolerant filial generation ismeasured, the salt-tolerant candidate gene is determined as a salt-tolerant re-selection gene, instantaneous conversion is conducted, a salt-tolerant physiological index of a salix matsudana seedlingis detected, and the salt-tolerant re-selection gene is determined as a salt-tolerant gene. According to the excavation method, the problem is solved that during traditional salt-tolerant salix matsudana breeding, the salt-tolerant gene cannot be accurately found out.

Description

technical field [0001] The invention relates to the technical field of forest tree breeding, in particular to a method for discovering salt-tolerant genes of willows. Background technique [0002] Salt-tolerant genes regulate the salt-tolerant phenotype of forest trees by regulating proteins. Therefore, the expression of salt-tolerant genes plays the most critical role in the salt-tolerant traits of forest trees. Salix matsudana (Salix matsudana) is an important species of Salix genus Salix. It is native to Northeast my country and is also known as Chinese willow. It plays an important role in soil heavy metal restoration, saline-alkali land greening, and biofuels. Afforestation along the eastern coast. However, traditional salt-tolerant dry willow breeding cannot accurately find salt-tolerant genes, so it cannot meet the needs of the development of salt-tolerant trees. Contents of the invention [0003] In order to overcome the defects in the prior art, a method for exc...

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the shortcomings of the existing technology, a method for excavating salt-tolerant genes of Salix sativa is now provided to solve the problem that traditional salt-tolerant willow breeding cannot accurately find salt-tolerant genes