38 results about "Salix matsudana" patented technology

The invention discloses a method for culturing agrobacterium-mediated transgenic salix matsudana plants and relates to the method for culturing the transgenic salix matsudana plants. The method aims to solve the problems that the existing method cannot induce resistant buds from transgenic transgenices of salix matsudana so that the transgenic salix matsudana plants cannot be obtained. The method comprises the following steps of: firstly, sterilizing and preculturing salix matsudana seeds; secondly, selecting agrobacterium tumefaciens with pBI121 carriers, and culturing to obtain a bacteria solution; thirdly, taking sprouted top bud parts cut from basic parts as explants, and then soaking in the bacteria solution; fourthly, taking out the explants and soaking up through filter papers, and inoculating the explants into a co-culture culture medium; fifthly, switching to a selective medium till the resistant buds grow, switching to a light condition, and screening the resistant buds; sixthly, transferring the resistant buds into an elongation culture medium; seventhly, cutting off stem sections, and switching to a rooting culture medium for culture till roots grow; and finally, carrying out polymerase chain reaction (PCR) and glucuronidase (GUS) staining detection, transplanting to earth, and obtaining the transgenic salix matsudana plants. The method for culturing the agrobacterium-mediated transgenic salix matsudana plants is applied to culturing the transgenic salix matsudana plants.

The invention relates to a pleurotus nebrodensis cultivation medium imitating wild cultivation, which is prepared from the following raw materials in parts by weight: 40-50 parts of lotus seed hulls, 20-30 parts of rural soil, 15-16 parts of pine nut hulls, 6-8 parts of bran, 10-12 parts of konjaku flour, 5-6 parts of bean dregs, 4-5 parts of pearl powder, 0.1-0.2 part of herba dendrobii powder, 1-3 parts of oyster powder, 1-2 parts of semen celosiae, 2-3 parts of salix matsudana koidz leaves, 1-2 parts of tribulus terrestris powder, 3-4 parts of rhizoma gastrodiae, 1-2 parts of radices hemerocallis, 1-2 parts of celastrus orbiculatus fruit powder, 6-8 parts of fishbone powder, 4-5 parts of zeolite powder, 1-2 parts of calcite powder, 0.5-1 part of glucose, 0.5-1.0 part of calcium superphosphate, 2-3 parts of quick lime, 20-21 parts of fish soup, 2-3 parts of gypsum powder and 1-3 parts of nutrient soil. The pleurotus nebrodensis cultivated by use of the cultivation medium is characterized by high content of mineral substances, trace elements, vitamins and amino acids; meanwhile, the pollution of heavy metals is reduced, so that the pleurotus nebrodensis has the functions of calming the liver and soothing nerves after a user eats the pleurotus nebrodensis for a long time; moreover, the growth is fast, and the yield is high.

The invention discloses an ecological method for repairing the concrete grating revetment of a river level fluctuation zone by using plants, which is characterized in that Salix matsudana Koidz which can resist flooding and acid as well as alkali is selected, the provenance (namely sprout branches) is selected in the local area or similar ecological habitat, and after indoor seedling raising by cutting, seedling transplantation and outdoor seedling acclimatization, the branches are planted in the concrete grating in the river level fluctuation zone in a bag cultivation way for adaptive growth. The ecological method ensures the survival rate of planted seedlings and the nutrition requirement for growth of the seedlings in the early stage, and plays an important role in improving the ecological quality of artificial hard revetments and landscapes at the river bank zone, thus having a good application prospect.

The invention discloses a method for improving the yield of hybrids in artificial hybridization of Salix matsudana. The method comprises the following steps: (1) selection of mother plants; (2) preparation of male flower shoots: selecting male plants with good hybridization affinity to Salix matsudana as male parents, wherein the male flower shoots are required to have diameters of 0.5-0.8 cm andlengths of 50-80 cm, and thinning the male flower shoots by removing 1/3 weak and small male flower buds; (3) preparation of female flower shoots, wherein the female flower shoots have lengths of 0.7-1.0 m and diameters of 1.0-2.0 cm; (4) culturing of the flower shoots; (5) pollen collection; (6) pollination; (7) management after pollination; and (8) hybrid collection. The method of the inventioncan effectively overcome the characteristic of thin and weak branches of Salix matsudana, improves pollen quality by thinning flowers, ensures the supply of nutrients in a hybrid development cycle through branch thinning and flower thinning, promotes the maturation of hybrids, increase the maturity and the yield of the hybrids, allows the yield and germination rate of the hybrids to be increased by 20% or more and 30% or more, respectively, and has high breeding success rate.

The invention discloses a method for exploring a stress-resistant rapid evolution gene of salix matsudana. The method for exploring the stress-resistant rapid evolution gene of salix matsudana can explore the stress-resistant rapid evolution gene of salix matsudana rapidly, efficiently and accurately, and a rich material basis is provided for salix matsudana genetic breeding to be applied to willow variety breeding with good stress resistance and strong adaptability. The method solves the problems of low mining efficiency and slow speed of traditional willow stress-resistant genes.

The invention belongs to the technical field of agrobiology and discloses a preparation method of intact chloroplasts of Salix matsudana leaves. The method comprises the following steps: the Salix matsudana leaves are collected and subjected to the following processes in sequence: 1) homogenization; 2) filtration; 3) preparation of coarse chloroplast precipitates; 4) suspension of the coarse chloroplast precipitates; 5) gradient preforming; 6) isodensity centrifugation; 7) recovery of the intact chloroplasts, and finally, suspension of the intact chloroplasts is obtained. With adoption of themethod, purity of the obtained chloroplasts is high, and envelope integrity can reach 80% or higher. The foundation is laid for conducting research of photosynthetic physiology and molecular biology of Salix matsudana at the chloroplast level, and one novel idea is provided for separation of organelles of other wood plants.

The invention discloses an operation suture with a function of antibiosis and a preparation technology thereof. The operation suture is prepared from 65-78 parts by mass of chitosan, 9-14 parts by mass of chondroitin sulfate, 3-6 parts by mass of indigofera tinctoria, 4-8 parts by mass of trichosanthes root, 6-9 parts by mass of peruvian groundcherry herb, 3-8 parts by mass of leaf of Chinese holly, 5-10 parts by mass of tangerine leaf, 7-11 parts by mass of rhizoma bletillae, 6-9 parts by mass of hypericum wightianum, 6-10 parts by mass of Stachys japonica, 6-10 parts by mass of petrocosmea duclouxii, 5-9 parts by mass of salix matsudana, a proper amount of dicumyl peroxide, a proper amount of sodium alginate, a proper amount of azone and a proper amount of menthol. The operation suture with a function of antibiosis is prepared from chitosan with substantial haemostasis effects and good biodegradability and common Chinese herbal medicines by refining, has good antibiosis effects and can effectively prevent operation wound infection.

The invention discloses a superior dry-land willow tree pollen in-vitro germination culture medium. Distilled water is used as a solvent, and the solvent contains 8-12 g/L of agar, 140-160 g/L of sucrose and 0.1-0.3 g/L of boric acid. The invention further discloses application and a preparation method of the superior dry-land willow tree pollen in-vitro germination culture medium and a method for determining the viability of dry-land willow pollen by adopting the superior dry-land willow tree pollen in-vitro germination culture medium. A determination result of the pollen viability determined by utilizing the superior dry-land willow tree pollen in-vitro germination culture medium and a pollen tube growth microscopic-observation technique is more stable and reliable.

The invention discloses a breeding method for salt-tolerant fast-growing seedlings of salix matsudana. The breeding method comprises the steps: 1) bamboo willow stock cutting and field planting, namely carrying out cutting propagation of bamboo willows on moderate saline and alkaline soil with the total salt content being 0.3%-0.5%, and then, carrying out transplanting and field planting; 2) salixmatsudana scion preparation, namely shearing annual branches with the diameters being 0.4cm-0.6cm before salix matsudana branches are germinated, and carrying out stratification for later use; 3) wedge grafting and main shoot changing, namely before sap starts to flow and a tree shoot is germinated at the end of March and at the beginning of April, selecting a bamboo willow with a straight trunkand the breast height diameter reaching 1.5cm-2.5cm, cutting the trunk at a high place, carrying out longitudinal chopping once on the center of the stock by using a chopper, keeping the depth of a chopping opening at 3cm-4cm, selecting standby robust salix matsudana branches without diseases, and cutting the salix matsudana branches to form 6cm-8cm wedged scions; strutting the chopping opening ofthe stock by using a grafting knife, and slightly inserting two scions to two sides inside the stock to make a stock forming layer aligned to a scion forming layer; and 4) management after grafting.By using the breeding method, the bred commercial seedling rate of the salix matsudana can be increased, and the nursery leaving time of commercial seedlings of the salix matsudana can be shortened.

The invention provides a slope surface dissected valley greening scour preventing system and a construction method thereof. The slope surface dissected valley greening scour preventing system comprises a varying slope arranged on a ramp. The varying slope is internally provided with a water gathering area. The front end and the rear end of the water gathering area in the gradient direction from low to high are each provided with a row of sand intercepting shrubs. Saliz matsudana wood piles arranged in a polygon manner and twisted and connected through connecting fixing ropes are inserted in asoil body of the water gathering area. Nutrient bars embedded in the soil body are arranged at the centers of the saliz matsudana wood piles, and each nutrient bar is composed of an outer shell with the surface provided with fertilizer outlet holes and an inner cavity filled with ecological matrix fertilizer. According to the slope surface dissected valley greening scour preventing system and theconstruction method thereof, construction is convenient, the unit layout is reasonable, the excellent greening scour preventing capability is achieved, the ecological environment protection slope surface and dissected valley ecological environment recovering function is achieved, long-term usage can be achieved under the situation that manual intervention is not needed, benefits are durably brought into play, solving of the problem about the high sand content of the water source in the high-sand-content water source area is facilitated, and the slope surface dissected valley greening scour preventing system and the construction method thereof are suitable for varying slope, dissected valley and channel interior water and soil loss treatment.

The invention relates to an insecticidal composition for caterpillar control. The insecticidal composition is prepared from following components by weight: 2 parts of ethofenprox, 2 parts of dipterex, 2.6 parts of asiatic moonseed rhizome, 3.7 parts of castor seed, 5.1 parts of nux vomica, 5 parts of mealy fangji root, 1.6 parts of mulberry leaf, 7 parts of solomonseal rhizome, 1.7 parts of coastal glehnia root, 4 parts of artemisia argyi, 2.6 parts of crystallized sodium sulfate, 1 part of cicuta douglasii, 4 parts of stemona root and 5.1 parts of common cnidium fruit. The insecticidal composition has good control effects for salix matsudana caterpillars and can play a role combining prevention and treatment.

The invention discloses a multiplication method of salix matsudana stems. The salix matsudana stems serving as an explant is used for multiplying the salix matsudana stems through a plant tissue culture method; during tissue culture, a culture medium comprises MS, naphthylacetic acid (NAA) of 0.01-1 mg/L, zeatin of 0.05-2 mg/L, saccharose of 30 g/L and agar powder of 6.5 g/L. According to the method disclosed by the invention, the salix matsudana stems can be quickly and stably multiplied; the operation is simple and easy to control; the multiplication rate is high.

The invention discloses a spleen-invigorating and qi-supplementing preparation for treatment of enteritis, which is prepared from the following herbal materials in parts by weight: 8-15 parts of eucommia barks, 13-19 parts of panax notoginseng, 12-15 parts of field sowthistle herbs, 8-12 parts of radix paeoniae alba, 7-16 parts of herba polygalae japonicae, 6-12 parts of nutmeg, 9-15 parts of prunella vulgaris, 12-17 parts of leaves of shrubby baeckea, 7-15 parts of swertia yunnanensis, 12-19 parts of herba hyperici japonica, 9-16 parts of radix pseudostellariae, 5-12 parts of salix matsudana, 13-15 parts of lily, 6-16 parts of herba ecliptae, and 5-11 parts of fritillaria. The preparation has good effects of invigorating the spleen, supplementing qi, and regulating qi to alleviate pain, can fundamentally eliminate focuses, restore the liver function and enhance the immunity, and has a definite therapeutic effect, therefore, the preparation has a relatively high popularization value. The preparation disclosed by the invention is developed on the basis of Chinese medicine treatment based on syndrome differentiation and in combination with modern latest scientific research achievements, and through the combination application of various herbal materials, the preparation has the characteristics of distinct curative effect, safety, convenience, and low price.

The invention discloses a method for improving heavy metal Cd transporting ability and resistance of plants by transforming SmZIP protein of salix matsudana. The method employs a genetic engineering technology to transfer a gene to a wild type tobacco, so that the gene can be specifically expressed in plant cells, and significantly improve the heavy metal Cd transporting ability and resistance ofplants. The method of the present invention has wide applicability, and a novel plant variety having high Cd transport ability and resistance can be obtained.

The invention provides a method for exploring a salt-tolerant hub gene of salix matsudana. The method comprises the following steps: hybridizing salt-tolerant salix matsudana with salt-sensitive salixmatsudana to obtain a filial generation F1 group, and dividing the filial generation F1 group into a salt-tolerant filial generation and a salt-sensitive filial generation; transcriptome sequencing is carried out on the root systems of the salt-tolerant filial generation and the salt-sensitive filial generation respectively to obtain differential expression gene sequences, and a high-density genetic map is constructed; salt tolerance QTLs positioning is carried out, and a corresponding QTL section is obtained through calculation; the QTL section and the differential expression gene sequence are compared with willow whole genome, a plurality of salt-tolerant candidate genes are obtained through analysis and the expression quantity of the salt-tolerant candidate genes is measured; a heterokinetic growth model is established, a system of ordinary differential equations is constructed, a regulation and control network is formed by the salt-tolerant candidate genes, and a salt-tolerant hubgene in the regulation and control network is determined. The problem that salt-tolerant genes cannot be accurately found by traditional salt-tolerant willow breeding is solved.

The invention provides salix matsudana 1, 7-sedoheptulose bisphosphatase as well as an encoding gene and an application thereof, and relates to the technical field of gene engineering. The gene is derived from salix matsudana and named as SmSBPase gene, wherein the sequence of the gene is shown as SEQ ID NO: 1, the protein encoded by the gene is 1, 7-sedoheptulose bisphosphatase, and the amino acid sequence of the gene is shown as SEQ ID NO: 2. The gene and the protein coded by the gene provided by the invention have the functions of 1, 7-sedoheptulose bisphosphatase, the salt tolerance of theobtained transgenic arabidopsis thaliana containing the SmSBPase gene is obviously improved, and excellent gene resources and enzyme resources are provided for the research of the salt tolerance of plants.

The invention discloses a Chinese herb preparation for treating the liver wind sputum carrying type tourette syndrome. Doctors of traditional Chinese medicine consider that the liver-yang generates wind syndromes, the liver-yang wind syndromes are circulated to have an influence on the head and eyes, and then the head rocks out of control; the tendons generate spasms due to the wind syndromes, the neck is stiff, and the limbs are vibrated; sputum is generated due to the wind syndromes, so that the sputum in the throat is generated. Traditional Chinese medicine including uncaria macrophylla, marigold, autumn zephyrlily herbs, cornu procaprae gutturosae, senna, turnip seeds, salix matsudana koidz, symplocos sumuntia roots, tylophora ovata, sunglo, echinopsis tubiflora and tabasheer which have the effects of stopping the wind and the spasms and eliminating phlegm by cooling are selected for preparing traditional Chinese medicine decoctions. Clinical tests in the hospital show that the total effective rate is 95.0%, the treatment effect is remarkable, and the Chinese herb preparation is worthy of application and popularization.