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38 results about "Salix matsudana" patented technology

Salix matsudana, the Chinese willow, is a species of willow native to northeastern China. The species is named in honour of Sadahisa Matsuda, a Japanese botanist. Three varieties are recognized, Salix matsudana var. anshanensis, S. matsudana var. matsudana, and S. matsudana var. pseudomatsudana. The species is widely cultivated in China, and a horticultural variant with twisted twigs and trunk, the corkscrew willow (S. matsudana var. tortuosa), is widely planted.

Method for culturing agrobacterium-mediated transgenic salix matsudana plants

The invention discloses a method for culturing agrobacterium-mediated transgenic salix matsudana plants and relates to the method for culturing the transgenic salix matsudana plants. The method aims to solve the problems that the existing method cannot induce resistant buds from transgenic transgenices of salix matsudana so that the transgenic salix matsudana plants cannot be obtained. The method comprises the following steps of: firstly, sterilizing and preculturing salix matsudana seeds; secondly, selecting agrobacterium tumefaciens with pBI121 carriers, and culturing to obtain a bacteria solution; thirdly, taking sprouted top bud parts cut from basic parts as explants, and then soaking in the bacteria solution; fourthly, taking out the explants and soaking up through filter papers, and inoculating the explants into a co-culture culture medium; fifthly, switching to a selective medium till the resistant buds grow, switching to a light condition, and screening the resistant buds; sixthly, transferring the resistant buds into an elongation culture medium; seventhly, cutting off stem sections, and switching to a rooting culture medium for culture till roots grow; and finally, carrying out polymerase chain reaction (PCR) and glucuronidase (GUS) staining detection, transplanting to earth, and obtaining the transgenic salix matsudana plants. The method for culturing the agrobacterium-mediated transgenic salix matsudana plants is applied to culturing the transgenic salix matsudana plants.
Owner:NORTHEAST FORESTRY UNIVERSITY

Pleurotus nebrodensis cultivation medium imitating wild cultivation and preparation method thereof

The invention relates to a pleurotus nebrodensis cultivation medium imitating wild cultivation, which is prepared from the following raw materials in parts by weight: 40-50 parts of lotus seed hulls, 20-30 parts of rural soil, 15-16 parts of pine nut hulls, 6-8 parts of bran, 10-12 parts of konjaku flour, 5-6 parts of bean dregs, 4-5 parts of pearl powder, 0.1-0.2 part of herba dendrobii powder, 1-3 parts of oyster powder, 1-2 parts of semen celosiae, 2-3 parts of salix matsudana koidz leaves, 1-2 parts of tribulus terrestris powder, 3-4 parts of rhizoma gastrodiae, 1-2 parts of radices hemerocallis, 1-2 parts of celastrus orbiculatus fruit powder, 6-8 parts of fishbone powder, 4-5 parts of zeolite powder, 1-2 parts of calcite powder, 0.5-1 part of glucose, 0.5-1.0 part of calcium superphosphate, 2-3 parts of quick lime, 20-21 parts of fish soup, 2-3 parts of gypsum powder and 1-3 parts of nutrient soil. The pleurotus nebrodensis cultivated by use of the cultivation medium is characterized by high content of mineral substances, trace elements, vitamins and amino acids; meanwhile, the pollution of heavy metals is reduced, so that the pleurotus nebrodensis has the functions of calming the liver and soothing nerves after a user eats the pleurotus nebrodensis for a long time; moreover, the growth is fast, and the yield is high.
Owner:HEFEI QIANXI MOUNTAIN VILLA AGRI ECOLOGICAL PARK

Purple sweet potato contained beef paste for moistening lung and tonifying spleen and preparation method thereof

InactiveCN104366433ARich tasteGood for invigorating the spleen and promoting dampnessFood preparationBiotechnologyPeppermints
The invention discloses purple sweet potato contained beef paste for moistening lung and tonifying spleen. The purple sweet potato contained beef paste disclosed by the invention is prepared from the following raw materials in parts by weight: 90-110 parts of purple sweet potatoes, 15-20 parts of bean halves, 10-15 parts of green pepper, 20-25 parts of honey, 7-8 parts of nutrition additives, 3-4 parts of peppermint candy, 6-7 parts of towel gourd powder, 5-6 parts of dried bean curd, 2-3 parts of duck oil, 10-15 parts of fresh beef, 8-9 parts of Fordia cauliflora, 4-5 parts of spirulina, 8-9 parts of papaya flesh, 8-10 parts of chicken, 7-9 parts of sesame oil, 10-12 parts of black sesame paste, 1.5-2 parts of caulis bambusae in taeniam, 2.8-3.3 parts of seeds of Chinese waxgourd, 2.1-2.7 parts of ammannia auriculata, 2.2-3 parts of morinda officinalis, and 2-2.3 parts of salix matsudana. According to the invention, the purple sweet potato is rich in mouth feel and also contains multiple health components; by means of addition of the components, such as ammannia auriculata, seeds of Chinese waxgourd and salix matsudana, the purple sweet potato contained beef paste has the good effects of tonifying spleen, promoting diuresis, promoting the circulation of qi, dissipating stasis, moistening lung, reducing phlegm, diminishing swelling, relieving pain and the like; and thus, the purple sweet potato disclosed by the invention has good health effects.
Owner:WUHE TONGSHIFU FOOD

Method for improving yield of hybrids in artificial hybridization of Salix matsudana

The invention discloses a method for improving the yield of hybrids in artificial hybridization of Salix matsudana. The method comprises the following steps: (1) selection of mother plants; (2) preparation of male flower shoots: selecting male plants with good hybridization affinity to Salix matsudana as male parents, wherein the male flower shoots are required to have diameters of 0.5-0.8 cm andlengths of 50-80 cm, and thinning the male flower shoots by removing 1/3 weak and small male flower buds; (3) preparation of female flower shoots, wherein the female flower shoots have lengths of 0.7-1.0 m and diameters of 1.0-2.0 cm; (4) culturing of the flower shoots; (5) pollen collection; (6) pollination; (7) management after pollination; and (8) hybrid collection. The method of the inventioncan effectively overcome the characteristic of thin and weak branches of Salix matsudana, improves pollen quality by thinning flowers, ensures the supply of nutrients in a hybrid development cycle through branch thinning and flower thinning, promotes the maturation of hybrids, increase the maturity and the yield of the hybrids, allows the yield and germination rate of the hybrids to be increased by 20% or more and 30% or more, respectively, and has high breeding success rate.
Owner:SHANDONG FOREST SCI RES INST

Method for improving salt tolerance of saliz matsudana

The invention discloses a method for improving the salt tolerance of saliz matsudana. The saliz matsudana and salix mongolica are crossed, hybrid seedlings are subjected to static cultivation, cuttings are made for water transplanting seedling cultivation, and salt concentration stress treatment is conducted after lignification of water transplanting seedling stems starts. The salt tolerance of the saliz matsudana is improved, the salt tolerance is increased to more than 0.5%, and the purpose of cultivating salt tolerant fast-growing willow is achieved. The disclosed hybridization breeding method significantly improves salt tolerance of hybrid offsprings by crossing salix mongolica male flower branches at suitable age and location with saliz matsudana. The method is simple to operate, easy to accept and master and low in cost and has good popularization and application value.
Owner:SHANDONG FOREST SCI RES INST

Method for raw soil planting in saline-alkali land by adopting rooting agent containing figwort root extract for planting salix matsudana

The invention provides a method for raw soil planting in saline-alkali land by adopting a rooting agent containing figwort root extract for planting salix matsudana. The method comprises the step of treating before planting. The step of treating before planting comprises the procedure of spreading the rooting agent. The rooting agent contains the following components: sodium naphthalene acetate, gamma-polyglutamic acid, ammonium chloride, soil activating agent, slow release agent, fungicide ridomil Mz, figwort root extract, illite powder, quartz sand powder, borax, protocatechuic acid, rice washing water, animal sclerotin calcium powder and / or animal marrow powder, magnesium oxide, anti-blocking agent and xanthan gum. According to the method for raw soil planting, provided by the invention, the survival rate of salix matsudana is greatly increased and the survival rate is 89.9-95.1%.
Owner:SHANDONG SUNWAY LANDSCAPE TECH

Excavation method for salt-tolerant gene of salix matsudana

The invention provides an excavation method for a salt-tolerant gene of salix matsudana. The method comprises the steps that salt-tolerant salix matsudana and salt-sensitive salix matsudana are crossbred to obtain an F1 population, the F1 population is divided into salt-tolerant and salt-sensitive filial generations, and through salt stress, root systems of the salt-tolerant and salt-sensitive filial generations are subjected to transcriptome sequencing to obtain differential expression gene sequences separately; based on the salt-tolerant filial generation, a high-density genetic map is established, salt tolerance QTLs positioning is conducted, a QTL section is obtained through calculation, the QTL section, the differential expression gene sequences and a salix matsudana whole genome arecompared, and through analysis, the salt-tolerant candidate gene is obtained; through salt stress, the relative expression of the salt-tolerant candidate gene of the salt-tolerant filial generation ismeasured, the salt-tolerant candidate gene is determined as a salt-tolerant re-selection gene, instantaneous conversion is conducted, a salt-tolerant physiological index of a salix matsudana seedlingis detected, and the salt-tolerant re-selection gene is determined as a salt-tolerant gene. According to the excavation method, the problem is solved that during traditional salt-tolerant salix matsudana breeding, the salt-tolerant gene cannot be accurately found out.
Owner:NANTONG UNIVERSITY

Breeding method for salt-tolerant fast-growing seedlings of salix matsudana

The invention discloses a breeding method for salt-tolerant fast-growing seedlings of salix matsudana. The breeding method comprises the steps: 1) bamboo willow stock cutting and field planting, namely carrying out cutting propagation of bamboo willows on moderate saline and alkaline soil with the total salt content being 0.3%-0.5%, and then, carrying out transplanting and field planting; 2) salixmatsudana scion preparation, namely shearing annual branches with the diameters being 0.4cm-0.6cm before salix matsudana branches are germinated, and carrying out stratification for later use; 3) wedge grafting and main shoot changing, namely before sap starts to flow and a tree shoot is germinated at the end of March and at the beginning of April, selecting a bamboo willow with a straight trunkand the breast height diameter reaching 1.5cm-2.5cm, cutting the trunk at a high place, carrying out longitudinal chopping once on the center of the stock by using a chopper, keeping the depth of a chopping opening at 3cm-4cm, selecting standby robust salix matsudana branches without diseases, and cutting the salix matsudana branches to form 6cm-8cm wedged scions; strutting the chopping opening ofthe stock by using a grafting knife, and slightly inserting two scions to two sides inside the stock to make a stock forming layer aligned to a scion forming layer; and 4) management after grafting.By using the breeding method, the bred commercial seedling rate of the salix matsudana can be increased, and the nursery leaving time of commercial seedlings of the salix matsudana can be shortened.
Owner:COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI

Slope surface dissected valley greening scour preventing system and construction method thereof

The invention provides a slope surface dissected valley greening scour preventing system and a construction method thereof. The slope surface dissected valley greening scour preventing system comprises a varying slope arranged on a ramp. The varying slope is internally provided with a water gathering area. The front end and the rear end of the water gathering area in the gradient direction from low to high are each provided with a row of sand intercepting shrubs. Saliz matsudana wood piles arranged in a polygon manner and twisted and connected through connecting fixing ropes are inserted in asoil body of the water gathering area. Nutrient bars embedded in the soil body are arranged at the centers of the saliz matsudana wood piles, and each nutrient bar is composed of an outer shell with the surface provided with fertilizer outlet holes and an inner cavity filled with ecological matrix fertilizer. According to the slope surface dissected valley greening scour preventing system and theconstruction method thereof, construction is convenient, the unit layout is reasonable, the excellent greening scour preventing capability is achieved, the ecological environment protection slope surface and dissected valley ecological environment recovering function is achieved, long-term usage can be achieved under the situation that manual intervention is not needed, benefits are durably brought into play, solving of the problem about the high sand content of the water source in the high-sand-content water source area is facilitated, and the slope surface dissected valley greening scour preventing system and the construction method thereof are suitable for varying slope, dissected valley and channel interior water and soil loss treatment.
Owner:甘肃省水利厅兰州水土保持科学试验站 +1

Spleen-invigorating and qi-supplementing preparation for treatment of enteritis and preparation method thereof

The invention discloses a spleen-invigorating and qi-supplementing preparation for treatment of enteritis, which is prepared from the following herbal materials in parts by weight: 8-15 parts of eucommia barks, 13-19 parts of panax notoginseng, 12-15 parts of field sowthistle herbs, 8-12 parts of radix paeoniae alba, 7-16 parts of herba polygalae japonicae, 6-12 parts of nutmeg, 9-15 parts of prunella vulgaris, 12-17 parts of leaves of shrubby baeckea, 7-15 parts of swertia yunnanensis, 12-19 parts of herba hyperici japonica, 9-16 parts of radix pseudostellariae, 5-12 parts of salix matsudana, 13-15 parts of lily, 6-16 parts of herba ecliptae, and 5-11 parts of fritillaria. The preparation has good effects of invigorating the spleen, supplementing qi, and regulating qi to alleviate pain, can fundamentally eliminate focuses, restore the liver function and enhance the immunity, and has a definite therapeutic effect, therefore, the preparation has a relatively high popularization value. The preparation disclosed by the invention is developed on the basis of Chinese medicine treatment based on syndrome differentiation and in combination with modern latest scientific research achievements, and through the combination application of various herbal materials, the preparation has the characteristics of distinct curative effect, safety, convenience, and low price.
Owner:王燕

The excavation method of the dry willow -resistant salt hub gene

The invention provides a method for exploring a salt-tolerant hub gene of salix matsudana. The method comprises the following steps: hybridizing salt-tolerant salix matsudana with salt-sensitive salixmatsudana to obtain a filial generation F1 group, and dividing the filial generation F1 group into a salt-tolerant filial generation and a salt-sensitive filial generation; transcriptome sequencing is carried out on the root systems of the salt-tolerant filial generation and the salt-sensitive filial generation respectively to obtain differential expression gene sequences, and a high-density genetic map is constructed; salt tolerance QTLs positioning is carried out, and a corresponding QTL section is obtained through calculation; the QTL section and the differential expression gene sequence are compared with willow whole genome, a plurality of salt-tolerant candidate genes are obtained through analysis and the expression quantity of the salt-tolerant candidate genes is measured; a heterokinetic growth model is established, a system of ordinary differential equations is constructed, a regulation and control network is formed by the salt-tolerant candidate genes, and a salt-tolerant hubgene in the regulation and control network is determined. The problem that salt-tolerant genes cannot be accurately found by traditional salt-tolerant willow breeding is solved.
Owner:NANTONG UNIVERSITY

An Agrobacterium-mediated method for cultivating transgenic willow plants

The invention discloses a method for culturing agrobacterium-mediated transgenic salix matsudana plants and relates to the method for culturing the transgenic salix matsudana plants. The method aims to solve the problems that the existing method cannot induce resistant buds from transgenic transgenices of salix matsudana so that the transgenic salix matsudana plants cannot be obtained. The method comprises the following steps of: firstly, sterilizing and preculturing salix matsudana seeds; secondly, selecting agrobacterium tumefaciens with pBI121 carriers, and culturing to obtain a bacteria solution; thirdly, taking sprouted top bud parts cut from basic parts as explants, and then soaking in the bacteria solution; fourthly, taking out the explants and soaking up through filter papers, and inoculating the explants into a co-culture culture medium; fifthly, switching to a selective medium till the resistant buds grow, switching to a light condition, and screening the resistant buds; sixthly, transferring the resistant buds into an elongation culture medium; seventhly, cutting off stem sections, and switching to a rooting culture medium for culture till roots grow; and finally, carrying out polymerase chain reaction (PCR) and glucuronidase (GUS) staining detection, transplanting to earth, and obtaining the transgenic salix matsudana plants. The method for culturing the agrobacterium-mediated transgenic salix matsudana plants is applied to culturing the transgenic salix matsudana plants.
Owner:NORTHEAST FORESTRY UNIVERSITY
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