Multiplication method of salix matsudana stems

A technology of willow and dragon's claw, which is applied in the field of plant tissue culture and forest tree cultivation, to achieve the effects of less secondary metabolites, controllable light, and simplified tissue culture operations

Inactive Publication Date: 2014-02-05
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there is no report on the pro...

Method used

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  • Multiplication method of salix matsudana stems
  • Multiplication method of salix matsudana stems
  • Multiplication method of salix matsudana stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0129] 1. Preparation of Medium

[0130] (1) Adjust the pH of the MS medium to 5.8 with NaOH, add 6.5 g of agar powder, sterilize at 121°C for 20 minutes, and cool to 50°C for use;

[0131] (2) Naphthaleneacetic acid and zeatin are sterilized by filtration with a filter membrane with a pore size of 0.22 μm, and are set aside;

[0132] (3) Add 0.08 mg of naphthaleneacetic acid and 1.2 mg of zeatin sterilized in (2) to the agar-MS prepared in step (1), set the volume to 1 L, and complete the solidification.

[0133] 2. Proliferation of willow stems

[0134] (1) Selection of explants, including the following sub-steps:

[0135] (1-1) In April, select the morphological upper 4 nodes of the stems of the current year's willow willow or the stems of the willow willow obtained by multiplying by the method provided by the present invention, each node is used as an explant, and stored in ice packs;

[0136] (1-2) Cut off the morphological upper end of the leaves on the explants obtai...

Embodiment 2

[0151] 1. The preparation of substratum and the multiplication process of willow stalks are the same as in Example 1, the difference is only the weight of adding naphthaleneacetic acid and zeatin when preparing substratum:

[0152] Naphthaleneacetic acid 0.05mg, zeatin 1.0mg.

[0153] 2. Proliferation result of willow stem

[0154] The axillary buds of the willow stem as explants begin to expand after 17 days of subculture, and new leaves are drawn out from the axillary buds after 25 days, grow to 3 cm in 38 days, and grow to 15 cm after 90 days, which is the unrooted tissue culture seedling of willow willow; The proliferation rate is about 1:1000.

Embodiment 3

[0156] 1. the preparation of substratum and the stem multiplication process of dragon's claw are the same as in Example 1, the difference is only the weight of adding naphthaleneacetic acid and zeatin when preparing substratum:

[0157] Naphthaleneacetic acid 0.6mg, zeatin 1.7mg.

[0158] 2. Proliferation result of willow stem

[0159] The axillary buds of the willow stem as explants begin to expand after 18 days of subculture, and after 27 days, the axillary buds take out new leaves, grow to 3 cm in 38 days, and grow to 15 cm in 88 days, which is the unrooted tissue culture seedling of willow willow; The proliferation rate is about 1:1000.

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Abstract

The invention discloses a multiplication method of salix matsudana stems. The salix matsudana stems serving as an explant is used for multiplying the salix matsudana stems through a plant tissue culture method; during tissue culture, a culture medium comprises MS, naphthylacetic acid (NAA) of 0.01-1 mg/L, zeatin of 0.05-2 mg/L, saccharose of 30 g/L and agar powder of 6.5 g/L. According to the method disclosed by the invention, the salix matsudana stems can be quickly and stably multiplied; the operation is simple and easy to control; the multiplication rate is high.

Description

technical field [0001] The invention relates to the field of tree cultivation, in particular to the field of plant tissue culture. Background technique [0002] Dragon claw willow, also known as dragon beard willow, Latin name: Salix matsudana var.tortuosa, family name: Salicaceae Salicaceae, is a deciduous shrub or small tree of the genus Salicaceae that grows in Northeast, North, Northwest, East China and other places , can grow in wetlands and dry lands, and grows fast. It has the characteristics of positive and cold resistance. It is suitable for spring to summer. Its branches are curly and unique. It is widely used in gardens and cultivated, but its quantity is limited, and it is not easy to propagate itself. Therefore, it is subject to certain restrictions in garden applications, and there is no good propagation method to propagate it on a large scale. [0003] The propagation methods of Longclaw willow include seeding method or cutting method. Among them, the sowing...

Claims

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Application Information

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IPC IPC(8): A01H4/00C05G3/00
Inventor 张建国饶国栋睢金凯
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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