Polypeptide self-assembled hydrogel stent and application thereof

A self-assembly and hydrogel technology, applied in the fields of immunology and biomedical engineering, can solve problems such as limiting cell extension, and achieve the effect of protecting retention time, enhancing immune response and improving effect.

Pending Publication Date: 2021-11-09
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing conventional cell culture methods meet the needs of cell nutrition, the two-dimensional space limits the extension of cells. Relatively speaking, the hydrogel three-dimensional scaffold can form a scaffold with a certain spatial structure by adjusting the concentration. The hydrogel can be fully stretched, and the biocompatibility of the hydrogel is good, which is conducive to the proliferation of cells and the exchange of information with other cells.

Method used

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  • Polypeptide self-assembled hydrogel stent and application thereof
  • Polypeptide self-assembled hydrogel stent and application thereof
  • Polypeptide self-assembled hydrogel stent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the self-assembled hydrogel scaffold of the present invention is as follows:

[0034] Step 1. Preparation of bonded functional polypeptides

[0035] The small molecule polypeptide carrier is bonded to the cell adhesion molecule functional mimic peptide at a molar ratio of 1:1 through a covalent bond, the covalent bond includes a disulfide bond, an amide bond or an amino acid sequence ( figure 1 ).

[0036] According to the same method, the cytokine function mimic peptide and the chemokine function mimic peptide are further used, respectively bonded with the small molecule polypeptide carrier through a covalent bond.

[0037] The small molecule polypeptide carrier described in this embodiment is a small molecule polypeptide of 10-20 amino acids, including: RADARADARADARADA, FEFKFEFKFEFKFEFK, KWKAKAKAKWK, EWEAEAEAE and the like. The above amino acids were purchased from sigma-aldrich Sigma-Aldrich (Shanghai) Trading Co., Ltd.

[0038]Step 2. P...

Embodiment 2

[0041] Preparation of Co-Assembled Bonding Functional Peptides

[0042] Mix the scaffold material containing cell adhesion molecule peptide and the scaffold material containing cytokine mimetic peptide in Example 1 at a ratio of 1:1, co-assembly occurs spontaneously, and changes from liquid to semi-solid as the concentration increases gel state to obtain self-assembled hydrogel scaffolds.

Embodiment 3

[0044] Hydrogel scaffolds promote dendritic cell maturation in vitro

[0045] Human peripheral blood mononuclear cells were isolated and added to Gibico1640 medium, the self-assembled hydrogel scaffold prepared in Example 2 was added to the medium, mixed and heated at 37°C, 5% CO 2 The cells were recovered after 48 hours of culture under low temperature, and the secretion of IL-12, a key cytokine for dendritic cell maturation, was detected by enzyme-linked immunoassay. The result is as Figure 4 As shown, the hydrogel scaffold can induce the maturation of dendritic cells.

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PUM

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Abstract

The invention discloses a polypeptide self-assembled hydrogel stent and application thereof. A micromolecule polypeptide carrier is bonded with functional mimetic peptides including cell adhesion molecule mimetic peptides, cytokine mimetic peptides and chemotactic factor mimetic peptides through covalent bonds to form a self-assembled nanostructure, and the nanostructure hydrogel stent with an immune cell collection function is formed through self-assembling in hydrogel by adjustment of the concentration of the hydrogel. The stent can be used for tumor treatment, injury repair and the like. The functional polypeptide is protected in the hydrogel stent in a three-dimensional existence form, so that in-vivo survival time is prolonged, and the functional polypeptide is in full contact with the immune cells so as to be suitable for recruitment and proliferation of the immune cells, a resistance and repairing effect for diseases, such as tumors, wounds and the like, which perform the function on the basis of the immune cells can be improved, and finally, tumor immunotherapy and wound repair effects are improved.

Description

technical field [0001] The invention belongs to the fields of immunology and biomedical engineering, and in particular relates to a polypeptide self-assembled hydrogel scaffold and its application. Background technique [0002] In the case of tumors, wound repair and other diseases, immune cells play a central role in recognizing tumor antigens, clearing tumor cells and residual inflammatory cells, and accelerating wound repair. While more antigenic tumor cells can elicit effective adaptive immune responses, less antigenic tumor cells can escape immune attack due to tumor immune escape. In addition, tumors can also reduce and lose the selective expression of tumor-specific antigens and tumor-associated antigens through antigen modulation, resulting in the greatly weakened ability of antigen-presenting cells to present antigens, evading the recognition and attack of the immune system, and unable to produce An adequate immune response clears the tumor. Antigen-presenting cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K7/06A61K9/06A61K47/64A61P17/02A61P35/00
CPCC07K7/08C07K7/06A61K47/64A61K9/06A61P35/00A61P17/02
Inventor 杨鹏翔接晶杨宇民张鲁中赵亚红彭勇
Owner NANTONG UNIVERSITY
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