2results about How to "Achieve multiplication" patented technology

The invention provides a method for preparing cellulase and application of the cellulase. The method for preparing the cellulase comprises the following steps: S1) improving the expression level of an SNC1 gene in trichoderma reesei to obtain trichoderma reesei engineering bacteria; s2, the trichoderma reesei engineering bacteria are cultured and collected to obtain the cellulase, the problem that in the prior art, a large amount of cellulase is difficult to produce through trichoderma reesei under the culture condition containing glucose can be solved, and the method is suitable for the technical field of microorganisms.

This invention discloses a humanized Sirpα The construction methods of immunodeficient mice and their application in human immune reconstitution belong to the field of medical experimental model construction technology. Rag2 ‑ / ‑ IL2rg ‑ / ‑ Using animals with T, B, and NK cell immune dysfunction caused by mutations as the background strain, sgRNA and dsDNA were specifically designed, and their endogenous components were processed using CRISPR / Cas9 gene editing technology. Sirpα The entire genome was modified to be humanized, thus creating a humanized genome. Sirpα Immunodeficient mice. This method is simple to operate, highly reproducible, and constructs a fully humanized mouse. Sirpα Immunodeficient mice exhibit a higher immunodeficient phenotype and can achieve a higher rate of human T cell chimerism after transplantation with human peripheral blood mononuclear cells, while effectively reducing the risk of graft-host disease.