The invention discloses a tissue culture and rapid propagation method suitable for tung trees. The method comprises the following processes: selecting explants, treating the explants, carrying out primary bud induction culture, carrying out propagation culture, culturing strong buds and carrying out rooting culture; the method comprises the concrete steps of collecting semi-lignified tung tree twigs, which grow robustly in the very year, as the explants, carrying out pruning and sterilizing treatment on the explants, and then inoculating an initial bud induction medium with the explants so as to obtain initial buds; inoculating a propagation medium with the initial buds, and carrying out the propagation culture for 3-4 cycles to form multiple shoots; inoculating a strong bud culture medium with the multiple shoots for culturing; finally, inserting a single bud with the height of more than or equal to 2cm into a rooting medium for inducing a root. After the tissue culture and rapid propagation method is used, the seedling raising period of the tung trees is shortened, and seedling raising materials are saved; the method solves the problems that the traditional seedling raising method needs more seedling raising materials, is long in cycle, and the like, greatly lowers the production cost, increases the production efficiency, and has better economic benefit, social benefit and ecological benefit.