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Superior dry-land willow tree pollen in-vitro germination culture medium and method for determining viability of dry-land willow pollen

A technology of in vitro germination and pollen vigor, applied in the biological field, to achieve stable and reliable results

Pending Publication Date: 2015-12-23
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the culture medium and culture conditions for the in vitro culture of willow pollen

Method used

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  • Superior dry-land willow tree pollen in-vitro germination culture medium and method for determining viability of dry-land willow pollen
  • Superior dry-land willow tree pollen in-vitro germination culture medium and method for determining viability of dry-land willow pollen
  • Superior dry-land willow tree pollen in-vitro germination culture medium and method for determining viability of dry-land willow pollen

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Embodiment 1

[0030] 1. Collection of pollen from the dry willow tree

[0031] Choose the willow plant (shang 19, shang 10 and Ken 1 of normal development, no damage by disease and insects, robust growth; among the present invention, shang 19, shang 10 and ken 1 represent the numbering of the dry willow excellent tree, wherein, "shang" represents "Shanghe", "Ken" represents "Kenli"), cut flower branches with a diameter of 1-2.5cm and a length of about 1-1.5m from the middle and upper part of the crown, and put them in a greenhouse (temperature 20-25°C, relative humidity 60- 70%) hydroponics; when a small amount of cooked pollen is scattered at the lower end of the male inflorescence, you can hold the inflorescence with clean white paper, shake the inflorescence gently with your hands, let the pollen scatter on the white paper, and then flick the pollen mixed in the pollen with a brush. For small sundries, pollen is purified and dried, wrapped in clean white paper and put into a desiccator (...

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Abstract

The invention discloses a superior dry-land willow tree pollen in-vitro germination culture medium. Distilled water is used as a solvent, and the solvent contains 8-12 g / L of agar, 140-160 g / L of sucrose and 0.1-0.3 g / L of boric acid. The invention further discloses application and a preparation method of the superior dry-land willow tree pollen in-vitro germination culture medium and a method for determining the viability of dry-land willow pollen by adopting the superior dry-land willow tree pollen in-vitro germination culture medium. A determination result of the pollen viability determined by utilizing the superior dry-land willow tree pollen in-vitro germination culture medium and a pollen tube growth microscopic-observation technique is more stable and reliable.

Description

technical field [0001] The invention relates to a culture medium for the in vitro germination of the pollen of a willow tree and a method for measuring the vigor of the pollen of the willow tree, belonging to the field of biotechnology. Background technique [0002] Dry willow (Salixmatsudana Koidz) is a tree of Salixaceae and Salix. Dry willow has soft branches and a full crown. It is a commonly used shade tree and street tree in northern China. It is often cultivated on the banks of rivers and lakes or isolated on lawns, and planted on both sides of buildings. It is also used as a road tree, a shelter forest and afforestation of sandy deserts, and greening of the "surroundings" in rural areas. It is a dense source tree species in early spring. Shelter forests and greening tree species can also be used as timber tree species. Beautiful tree shape, easy to reproduce, deeply loved by people. Its soft and tender green branches, plump crown and slightly pruned tree posture ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12Q1/02
Inventor 乔玉玲秦光华宋玉民姜岳忠康智于振旭
Owner SHANDONG FOREST SCI RES INST
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