46results about How to "The measurement results are accurate and reliable" patented technology

The invention relates to high optical spectral image technique without harm to agricultural products. It can reflect the appearance of the agriculture products like color, shape, texture, dimension, scar and son on, and internal features like hardness, protein content and connected with knowledge base and experience of experts to make judgment. It can make quick, accurate, timely judgment of products, controlling the overall production with guarantee of the agriculture quality.

The invention discloses a detection method for the expression of a GPR120 gene based on eGFP. The detection method comprises the following steps: inserting an eGFP fragment to the specific site TAA of the termination codon of the GPR120 gene by using CRISPR / Cas 9 technology so as to obtain a transgenic model mouse with eGFP-labeled GPR120 positive cells; and carrying out excitation by using a fluorescence analyzer and determining the fluorescence intensity of the positive cells of the mouse. The method carries out real-time monitoring on the expression of the GPR120 gene so as to control and reduce errors among groups, guarantee the reliability of results and compensate for and overcome the problems of variability caused by comparison of different groups of histocytes and incapability of monitoring the changes of gene expression at a living cell level in the prior art. According to the invention, the expression level of the GPR120 gene can be immediately determined after collection of cells, and operations and reactions like RNA extraction, inverse transcription and PCR are omitted, so detection of the expression of the GPR120 gene is simpler.

The present invention is continuous drilling flow rate capable of predicting coal draft outburst and its apparatus. By means of the method of drilling holes in the coal bed drilling direction for data acquisition and the apparatus comprising hole sealing capsule unit, solid-gas separator, filter, coal bit controller, electric rock drill with connected displacement sensor LXC, controller unit KZ with explosion resistant enclosure and connected computer data acquisitor SC, the present invention can determine the gas distribution in the drilling direction, obtain the gas flow rate distribution curve along the drilling depth during coal bed drilling process, so as to determine the distance of soft coal containing high pressure gas to the ground, judge the distance to the place with maybe outburst. The present invention has compact reasonable structure, high accuracy and stable and reliable measurement result, and may be used widely.

The present invention is the method of detecting starch content in ash tree flower polysaccharide product. The detection includes the steps of eliminating liposoluble component, eliminating soluble saccharide as interference matter, enzyme decomposing starch, detecting starch, processing enzyme hydrolyzed starch liquid, Fehling's process of determining reducing sugar content, etc. The method ofthe present invention is suitable for operation, has stable and reliable determined result, small variation coefficient of determined result, less error, high repeatability and other advantages.

The invention discloses a quality control method of a peach kernel qi activating decoction composition and belongs to the field of traditional Chinese medicine analysis. The method includes the steps that a peach kernel qi activating decoction fingerprint spectrum is established through high performance liquid chromatography, and chromatographic conditions are characterized in that a chromatographic column adopts octadecylsilane chemically bonded silica as filler; an ultraviolet detector is adopted as a detection instrument, and the detection wavelength of the fingerprint spectrum ranges from 210 nm to 250 nm; the flow rate ranges from 0.9 ml / ml to 1.1 ml / ml, the column temperature ranges from 25 DEG C to 35 DEG C, the detection wavelength ranges from 210 nm to 250 nm, the number of theoretical plates should not be smaller than 3,000 according to calculation of catechinic acid peaks, methyl alcohol serves as a moving phase A, a 0.1% phosphoric acid solution serves as a moving phase B, and gradient elution is carried out according to the following sequence: the gradient condition of elution detection of the fingerprint spectrum is defined in the specification. The fingerprint spectrum comprehensively reflects quality information of a peach kernel qi activating decoction, and therefore the quality of the peach kernel qi activating decoction can be more comprehensively and effectively controlled.

The invention relates to method to detect the N20 release of soil-plant system. The invention uses the gas phase chromatography-isotope ratio mass spectrometer coupled device and encases the detected object by the transparence plastic bag. It replaces the bag to remove the N20 in human confecting air, so the N20 in collecting sample would all be released by the detected object to measure the delta15N of N20 in soil, plant and soil-plant system, so it can get the N20 contributing ration of the plant in soil-plant system by the formula. So the method in the invention is accurate to detect the N20 released in soil, plant and soil-plant system.

The present invention discloses the method of detecting water soluble active polysaccharide content in ash tree flower polysaccharide product. The detection includes the steps of eliminating liposoluble component, eliminating soluble saccharide as interference matter, sulfuric acid-phenol process of determining active polysaccharide content, etc. The method of the present invention is suitable for operation, has stable and reliable determined result, small variation coefficient of determined result, less error, high repeatability and other advantages.

The invention discloses an analytical reagent for measuring heavy metal. The analytical reagent has a chemical structure with a general formula (I) and can react with lead, cadmium and mercury to generate stable complexes, the complexes can be separated on a reversed phase liquid chromatographic column, the trace amount of lead, cadmium and mercury in an environmental sample can be accurately measured by a visible photometric detector, and the limit of detection of the method reaches ng / L level. The measuring results obtained by using the analytical reagent coincide with those obtained by adopting the inductively coupled plasma mass spectrum (ICP-MS), which shows that the measuring results are reliable.

The invention relates to application of biogenic amine serving as a feature mark in bee venom type identification and further relates to a method for identifying types of bee venoms through the biogenic amine as the feature mark by means of HPLC / MS / MS methods. According to the method, one or more of histamine, dopamine, serotonin, epinephrine and noradrenaline serves as the feature mark, one or more of Italian bee venom, royal jelly high-yielding bee venom, Anatolia bee venom, Caucasia bee venom, Carniolan bee venom and Carpathian bee venom can be precisely identified. The method can efficiently, stably and accurately identify six types of bee venoms, and has significance on reinforcing study on bee venom type identification, exploring bee venom traceability information, developing traditional Chinese medicine, improving bee venom quality in China and guaranteeing health of consumers.

The invention discloses an off-line detection device of ice nucleus concentration and ice formation activity under nucleus infiltration mode and a detection method thereof. The device of the inventioncomprises a liquid separator, a PCR board, a PCR support, a water bath, a computer, a lens and a CCD camera. Temperature control, temperature recording, image acquisition and data processing modulesare arranged inside the computer. The PCR board is placed on the PCR support, and the PCR board and the PCR support are placed together in a condensate liquid in the water bath. The water bath and theCCD camera are connected to the computer. Image acquisition, image storage and data processing are controlled through the image acquisition module. according to the invention, a liquid containing groove can contain more liquid drops; loss of particles to be detected is reduced; and by the use of the PCR board, the problem that liquid drops influence each other during the cooling process is reduced, measurable liquid drop volume is increased, the difficulty of liquid separation is lowered, and errors caused by inaccurate liquid drop volume quantitation are reduced. The device of the inventionis simple to operate, and temperature recording, image acquisition and data processing can be conducted simultaneously.

The invention discloses a quality control method of a danggui sini decoction composition, wherein the method belongs to the field of traditional Chinese medicine analysis. According to the method of the invention, a fingerprint spectrum is established by means of high-efficiency liquid chromatography; a chromatographic column is a octadecylsilane chemically bonded silica chromatographic column; the column temperature is 25-35 DEG C; the flow velocity is 0.9-1.1ml / min; the detecting wavelength is 220nm; acetonitrile (A)-0.1% phosphoric acid solution (B) is used as a mobile phase, and gradient elution is performed according to a sequence which is represented in the description. The fingerprint spectrum according to the invention comprehensively reflects quality information of the danggui sini decoction composition, thereby realizing a purpose of more comprehensively and effectively controlling the quality of the danggui sini decoction composition.

The invention relates to a field returning crop straw nitrogen fate quantitative measurement method and belongs to the farmland nutrient cycling technology field. The method comprises steps that a first experiment plot and a second experiment plot isolated from external soil are preset, crops are grown on the first experiment plot, highly abundant 15N-labeled nitrogen fertilizer is applied at a crop growth period, the crops absorb the nitrogen fertilizer, straw marking is carried out after harvest, the marked straw is moved to the second experiment plot for field returning, later crops are then grown, 15N contents left in the marked straw, absorbed by the later crops and contained in the soil are measured at a critical growth period and after harvest, and dynamic field returning crop straw nitrogen conditions and fate thereof can be quantitatively monitored. The method is advantaged in that a measurement result can relatively visually reflect the nitrogen fate, the method is stable and reliable, external factor interference can be avoided, and technical supports are provided for understanding and researching field returning crop straw nitrogen release, mineralization characteristics and crop utilization characteristics.

The invention relates to a method for detecting activation of microencapsulated cells. The detection comprises the steps of sample extraction and phosphagen separation and quantification. The activation of the cell in a representation microcapsule is calculated through energy charge. The invention designs an operation scheme for the phosphagen quantification of microencapsulated cells to such special microcapsule system, which can accurately quantify the phosphagen content in the microencapsulated cells, and avoids the defects in the prior art such as large sample loss during extraction, unstable test, bad repeatability, etc., and the test results can be regarded as the evaluation indexes for the activation of the microencapsulated cells.

The invention discloses a liquid chromatographic method for separating and determining multiple impurities in tolvaptan. High performance liquid chromatographic detection conditions are that detection wavelength is 230 to 280 nm, a chromatographic column is Symmetry shield RP18 and a mobile phase is acetonitrile-aqueous solution containing 0.2% phosphoric acid, wherein the volume fraction of acetonitrile is 43 to 47%. The method provided by the invention overcomes problems in separation and determination of tolvaptan and five impurities thereof, especially problems in separation and determination of positional isomer impurities of tolvaptan and positional isomer impurities of tolvaptan intermediates; and the method has the advantages of good specificity, simplicity, rapidness, accuracy and accurate and reliable determination results, so the quality of tolvaptan can be effectively monitored, and drug security is improved.

The invention discloses a method for determining the content of sulfur in water. The method comprises the following steps: 1, acidity adjustment; 2, raw material supplementation; 3, precipitation; 4,filtration; 5, drying; and 6, calculation of the content C of sulfate radicals (SO<4><2->). The method is suitable for ground water, underground water, salt-containing water, domestic sewage and industrial wastewater, and can accurately determine a water sample with the sulfate content being 10 mg / L (by (SO<4><2->) or more, and the determination result is accurate and reliable.

The invention discloses a method for screening a plant variety with a leaf strengthening function. The invention belongs to the fields of breeding of crop high-lighting-effect and high-temperature-resistant variety, and relates to a method for screening a high-light-effect corn variety by a technological system, wherein the technological system is to screen a large amount of corn combination by applying leaf temperature, difference between leaf temperature and air temperature, leaf chlorophyll content, leaf nitrogen content and a fluorescence parameter PI (abs), select about 8 parts of materials, measure the photosynthetic rate by an LI-6400 photosynthesis system at the daily maximum temperature being 35 DEG C or above and select 3 to 4 parts of materials again to measure parameters such as photoresponse and carbon dioxide response. Compared with other breeding methods, the technology has the characteristic of screening a large amount of corn varieties, is suitable for selecting and breeding a high-quality corn variety from a large amount of varieties and has a good application prospect.

The invention relates to a device for automatic determination of the emulsion breaking degree of an oil product and a determination method. In the existing equipment for determination of the emulsionbreaking degree of the oil product, the automation degree is low, a laboratory technician needs to observe the oil-water separation condition in the whole process and the working efficiency is not high. The determination method comprises the following steps: by utilization of the Lambert-Beer law, stable monochromatic light transmits through a measuring cylinder containing the oil product, due todifference of light transmittance of the oil product, water and stirred emulsion, the transmitted lights are different in intensity, the light transmittance of each layer can generate jump, the jump part of the light transmittance is adopted as a dividing part of the layer and another layer, the height of each layer is equal to a product between the moving time of transmitting pools approximatelyequal in light transmittance multiply the moving speed of the transmitting pools, a control system calculates out the height of each layer automatically so as to judge a test endpoint. A lightproof cover with manual opening and closing is also designed at the periphery of a water bathtub, the test endpoint can be artificially judged by pulling and opening the lightproof cover and selecting a manual mode. The device and the determination method have the advantages that unattended determination for the emulsion breaking degree of the oil product is realized and the automation level of the determination process is improved.

The invention discloses an analytical reagent for heavy metal measurement as well as a preparation method and application thereof. The heavy metal refers to lead, cadmium and mercury, in particular to a trace amount of lead, cadmium and mercury. The new analytical reagent provided in the invention has the chemical structure of the general formula (I) in the specification. The synthesis of the analytical reagent is synthesized through two steps of: coupling diazotized aniline with 8-aminoquinaldine to obtain 5-phenylazo-8-aminoquinaldine, and then condensing the 5-phenylazo-8-aminoquinaldine with 2,4-dyhydroxylbenzaldehyde to obtain a product of 5-phenylazo-8-(2,4-dyhydroxylbenzaldehyde)-condensed aminoquinaldine. In the invention, based on the newly synthesized analytical reagent, a new method for measuring the trace amount of lead, cadmium and mercury with high performance liquid chromatography is established and has the detection limit up to a mu g / L level, and a measurement result of the method is provided to be identical to a result of an inductive coupling plasma mass spectrum (ICP-MS) method through contrast.

The invention discloses a method for measuring the concentration of aniline in the aqueous solution, comprising the following steps: (a) adding 1-25ml of aqueous solution to a volumetric flask of 25-100ml, adding 1-10ml of acid solution and 1-10ml of sodium nitrite solution in sequence, shaking uniformly, and standing for 1-10min for later use; (b) adding1-10ml of amino sulfonic acid ammonium to the solution of later use, fully vibrating, and standing for 1-10min until the foams are eliminated to prevent the sodium nitrite from affecting the measurement; and (c) adding 1-10ml of chromogenic reagent into the prepared solution, shaking uniformly, metering the volume by using distilled water, standing for 1-10min, and comparing color at the wavelength of 500-550nm. The method for measuring the concentration of aniline in the aqueous solution is simple, can obtain a stable and reliable measurement result with good repeatability, high degree of precision and high degree of accuracy and particularly can ensure that the stability of the chromogenic product is improved by more than 2 times, thereby being an ideal method for measuring the concentration of low-concentration aniline in industrial water and sewage water.

The invention discloses a noninvasive blood glucose assay method. The method comprises the steps that the current absorption rate B1 of a preset position for infrared light with the wave length of lambda1 is tested, and then the current absorption rate B2 of the preset position for infrared light with the wave length of lambda2 is tested; according to B1, B2, a pre-stored initial blood glucose concentration value A0, the initial absorption rate A1 of the preset position for the infrared light with the wave length of lambda1 and the initial absorption rate A2 of the preset position for the infrared light with the wave length of lambda2, the current blood glucose concentration value D0 is calculated, wherein D0=A0*(B1-B2) / (A1-A2)+k; k is constant, and 0<=k<=0.5. The invention further discloses a noninvasive blood glucose assay system. When the blood glucose concentration of a human body is tested, water interference and human body interference are removed, the assay result is accurate and reliable, repeatability is good, sensitivity is high, and good assay result specificity on the specific test human body is achieved.

The invention discloses a bioactivity detection method and application of recombined humanized neuroglobin. The detection method disclosed by the invention comprises the following steps: 1) preparing the recombined humanized neuroglobin to be detected into a sample solution of the set concentration; 2) detecting the capability of the sample solution in eliminating radicals, and using the superoxide anion clearance rate through rhNgb, the hydrogen oxide clearance amount through rhNgb and / or the hydroxyl radical clearance rate through rhNgb as detection indexes; and 3) computing the detection data and comparing the detection data with the bioactivity standard data of the recombined humanized neuroglobin to obtain the detection result. The invention also provides the application of the neuroglobin Ngb in the preparation of a medicament with a function of eliminating the radicals, which provides the train of thoughts for the development of a new medicament. The detection method disclosed by the invention is stable, has the advantages of reliable detection result, high repeatability of the detection data and the like and can be widely applied to tracking the activity of the rhNgb and biological products containing rhNgb, and the detection result can better reflect the bioactivity of rhNgb proteins.

The invention provides a device for measuring an internal structure of coke and a measuring method thereof; the device comprises a microscope and a computer; the computer is used for controlling rotation of a polarizer of the microscope and front, rear, left, right, upper and lower three-dimensional moving of an object stage. The measuring method comprises the following steps: pretreating a to-be-tested sample for standby application; establishing the relationship between pixel values of a digital image of a substance and the reflectivity; shooting digital images for analysis of sample bireflectivity and optical anisotropic indexes; determining an effective point classification statistical rule; and analyzing an optical structure of the coke by the statistical rule. The device and the method have the advantages that compared with an image analysis method purely relying on gray and shape characteristics, the method adopts three parameters of the maximum reflectivity, the bireflectivity and the optical anisotropic indexes for identification and analysis of the optical structure, and the measured results are more accurate and more reliable; and the whole analysis process is fully automatic, and the method is a fast and efficient analysis method.

The invention discloses a chromatographic method for determining the content of EPA, DHA and total w-3 acids in fish oil. The method comprises the following steps: 1, esterifying; 2, preparing a sample solution; 3, preparing a standard substance solution; 4, setting chromatographic conditions; 5, sequentially introducing samples; and 6, comparing the relation between the retention time and the peak area of a sample to be determined with that of a standard substance to determine the content in the sample. In the w-3 fatty acid-containing fish oil sample treatment process, unsaturated fatty acids is saponified, saponified unsaturated fatty acids and a methanol solution of boron trifluoride directly undergo an ester formation reaction, and unsaturated fatty acid esters are directly extracted by a BHT-containing isooctane solution to obtain a solution used as the sample to be determined. The method adopting the appropriate ester formation process and the sample post-treatment process has the advantages of short test operation cycle, effective avoiding of volatilization and other problems in the operation, and accurate and reliable determination result, and is especially suitable for industrial production and other occasions needing large scale determination.

The invention discloses a method of determining major elements in ferrotitanium through an X-fluorescence fuse piece method. The method comprises the steps that (1) multiple standard samples with granularity being 200 meshes are selected; (2) the standard samples are weighed, and a composite oxidant and a viscosity reducer are added for uniform stirring; (3) a fusing agent is weighed into a platinum crucible, a standard sample obtained after uniform mixing is poured into the platinum crucible and covered with the fusing agent, and the platinum crucible is placed in a muffle furnace to completealloy sample pre-oxidation; (4) the platinum crucible subject to pre-oxidation is placed in a melting furnace for melting, a release agent is added for rotary shaking before release, and then coolingrelease is performed to prepare a glass sheet for analysis; (5) an X-ray fluorescence spectrometer is adopted to test the strength of the major elements in the standard sample, and tested strength values and standard values are made into a primary work curve; and (6) a to-be-detected sample of the blocky ferrotitanium is ground to have the same granularity as the standard sample and is melted into a glass sheet according to the steps (2) to (5), and the X-ray fluorescence spectrometer is used to analyze component values of the to-be-detected sample. The method can be used for determining theelements in the ferrotitanium with high titanium content, the life of the platinum crucible is prolonged, and result accuracy and precision are guaranteed.

The invention discloses a method for determining sulfate radicals in organic amine absorption liquid in the process of renewable wet flue gas desulphurization, which belongs to the field of flue gas desulfurization. For solving the problem that existing detection methods are long in time consuming, cumbersome in operation, high in sample requirements, and largely affected by alkali metal, the invention provides a method for determining sulfate radicals in organic amine absorption liquid, which is simple and rapid in process, and accurate and reliable in measurement results. The method is implemented by taking alizarin red as an indicator through determining p(SO2-4) in a desulfurization absorbent by using BaCl2 in a direct titration mode. According to the method, the process is simple and rapid and the measurement results are accurate and reliable.

The invention discloses a corn seed vitality determining method based on Ca<2+> flow rate. According to the method, the Ca<2+> flow rate at the embryonic root ends of corn seed embryos is determined by utilizing a non-damage micro-measurement system, the vitality of corn seeds is evaluated according to the Ca<2+> flow rate, and the flow rate is in extremely significant positive correlation with a seedling dry-weight vitality index. The method can be used for accurately determining the vitality of corn seeds, and compared with an existing detection method, the method needs shorter time and has higher efficiency.

The invention discloses a method for analyzing the content of hydroxypropyl-beta-cyclodextrin in a butylphthalide sodium chloride injection, which adopts a high performance liquid chromatography, and the chromatographic conditions are as follows, a cyano bonded silica gel chromatographic column is used as a chromatographic column, 5mmol / L ammonium acetate is used as a mobile phase A, acetonitrile is used as a mobile phase B, the ratio of the mobile phase A to the mobile phase B is (50: 50), isocratic elution is performed, the flow velocity is 1.0 ml per minute, the column temperature is 20 DEG C, a detector is an evaporative light scattering detector, and parameters of the detector are as follows, carrier gas pressure is 0.5 Mpa, drift tube temperature is 70 DEG C, carrier gas flow velocity is 2.0 ml / min, and a shunting mode is adopted. The method can be used for accurately, simply, conveniently and efficiently measuring the content of the hydroxypropyl-beta-cyclodextrin in the butylphthalide sodium chloride injection.

The invention discloses a real-time dynamic light scattering quantitative PCR instrument including: a thermal circulation system used for controlling heating status of a PCR sample test tube; a dynamic light scattering detection unit, which includes an irradiation part that irradiates a light from a light source onto the PCR sample test tube, a spectrum intensity acquiring part and a measuring part, wherein the spectrum intensity acquiring part splits a reflecting light from a reference surface and a scattered light which transmits through the reference surface and comes from the sample, thusobtaining the spectrum intensity of the reflecting light and an interference light thereof, then the measuring part, on the basis of the spectrum intensity, performs detection on real-time dynamic light scattering change to the PCR sample; a control system, which includes a control unit, and an upper computer that analyzes and processes a detection data coming from the measuring part. The method can eliminate interference on the detection due to nonspecific PCR products, and can be used for observing the increase status of a PCR amplified product in the reaction tube in real time, is high in automatic degree and is more reliable in detection result.

The invention discloses a method for simultaneously measuring iodine content and bromine content in soil. According to the method comprises the following steps that: a sample and sodium carbonate areuniformly mixed, and then are covered with sodium carbonate again; the sample and the sodium carbonate are subjected to high temperature sample melting; a white substance in a content obtained throughsample melting is dissolved with water; water is added; so that the volume of an obtained mixture can be constant and reaches a certain volume; the mixture stands still, a supernatant is taken; an antioxidant can be added or not added; positive ion exchange resin is added, an obtained mixed solution is shaken up; water is added to dilute the solution; an obtained mixed solution is shaken up; thesolution is adopted as a to-be-measured solution; a blank solution and the to-be-measured solution are measured through ICP-MS, the mass concentrations of iodine and bromine in the blank solution andthe to-be-measured solution are obtained according to the working curves of iodine and bromine; and the mass concentrations are converted into the contents of iodine and bromine in the sample according to a formula. According to the method, a single sample melting reagent is adopted, so that reagent blank is controllable, and therefore, a determination result is more effective and reliable. The method provided by the invention has the advantages of high precision, high accuracy, lower detection limit and wide linear range, and meets the requirement of the simultaneous detection of iodine and bromine in soil samples in batches.

The invention discloses a rapid identification method for cotton verticillium wilt resistance in seedling stage. The rapid identification method comprises the following steps: inoculating greensickness bacteria to cotton seedlings, measuring SPAD value of inoculated cotton leaves and calculating greensickness stress index, and then judging the cotton verticillium wilt resistance according to the greensickness stress index. According to the method, the cotton leaves are not damaged, the method is not disturbed by man-made subjective judgment factors, the measurement result is more reliable compared with a disease index method, the method is simpler and more convenient compared with a method for measuring the activity of cotton seedling greensickness related enzyme, and the measured target is unique. The detection method can rapidly identify the disease resistance of cotton verticillium wilt in each growth stage, the breeding process of varieties with cotton verticillium wilt is quickened, and the detection method can be directly popularized and applied to production.