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Method for detecting active polysaccharide content in ash tree flower polysaccharide

A technology of Grifola frondosa polysaccharide and active polysaccharide, which is applied in the direction of chemical analysis by titration, and achieves the effects of high reproducibility, small error and small coefficient of variation

Inactive Publication Date: 2004-02-11
LAIYANG AGRI COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the deficiencies in the existing method "GB / T 15672-1995 Determination of Total Sugar Content of Edible Fungi" and how to further improve and perfect it have become the most important method for the quantitative determination of the active ingredient polysaccharide content in the current Grifola frondosa polysaccharide products. Problems to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Removal of fat-soluble components: Take the dried and ground active polysaccharide samples (No. 1) to be tested, with masses of 1.0727 grams, 1.1028 grams, 1.1509 grams, 0.948 grams, 1.1813 grams, and 1.0481 grams, respectively, and put them in 6 Add 30ml of ether to each centrifuge cup, shake fully for 12 minutes, centrifuge at 4500 rpm for 8 minutes, discard the supernatant, then add 30ml of ether, and repeat the centrifugation for 3 times;

[0018] 2) Remove soluble carbohydrate interfering substances: add 30 ml of 80% ethanol at 58° C. to the centrifuge cup after discarding the supernatant, shake fully for 15 minutes, centrifuge at 5000 rpm for 10 minutes, discard the supernatant, Repeat this centrifugation for 3 times; then add 30ml of 50°C distilled water to the centrifuge cup after discarding the supernatant, shake in a constant temperature water bath at 50°C for 60 minutes, centrifuge at 5000 rpm for 5 minutes, collect the supernatant, and then Repeat centrif...

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Abstract

The present invention discloses the method of detecting water soluble active polysaccharide content in ash tree flower polysaccharide product. The detection includes the steps of eliminating liposoluble component, eliminating soluble saccharide as interference matter, sulfuric acid-phenol process of determining active polysaccharide content, etc. The method of the present invention is suitable for operation, has stable and reliable determined result, small variation coefficient of determined result, less error, high repeatability and other advantages.

Description

(1) Technical field [0001] The invention relates to a method for determining the polysaccharide extract of Grifola frondosa frondosa, in particular to a method for quantitatively determining the content of water-soluble polysaccharides with biological activity in the polysaccharide extract of the deep layer liquid fermentation broth of Grifola frondosa. (2) Background technology [0002] The polysaccharide extract of Grifola frondosa deep liquid fermentation broth mainly includes active ingredient polysaccharides and inactive ingredient starch; wherein, the quantitative determination of the active ingredient polysaccharide content is important for the quality control of the active polysaccharide products of Grifola frondosa and the determination of the production process of polysaccharide products. very necessary. However, the control of product quality standards is difficult to implement due to the existence of inactive ingredient starch in the biopolysaccharide extract. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16
Inventor 隋方功宋爱荣张玉娜吕银燕
Owner LAIYANG AGRI COLLEGE
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