209results about How to "Small coefficient of variation" patented technology

The invention relates to functional nanometer particle composite non-crosslinking microsphere powder, and a preparation method and application of the functional nanometer particle composite non-crosslinking microsphere powder. The functional nanometer particle composite non-crosslinking microsphere powder contains functional nanometer particle composite non-crosslinking microspheres; the functional nanometer particle composite non-crosslinking microspheres contain functional nanometer particles and polymers, average particle size is 0.1micron to 20micron, and particle size distribution variable coefficient is less than or equal to 9.1%. The preparation method is a combination of a membrane emulsification technique and a solvent volatilization method. The invention also relates to a biometric probe and application of the biometric probe based on the above composite non-crosslinking microsphere powder. The preparation method has the advantages that the functional nanometer composite non-crosslinking microsphere powder which is uniform in particle size can be prepared; the prepared functional nanometer composite non-crosslinking microspheres belong to micron order, the particle size variable coefficient is small, monodispersity is good, and performance is excellent; and the prepared functional nanometer composite non-crosslinking microspheres have broad application prospect in biometric and biomedical fields and the like.

The invention relates to qualitative and quantitative detection dual-purpose HCG test paper for colloidal gold immunochromatography assay. The test paper comprises a combined pad and an analysis membrane, wherein HCG monoclonal antibody complex labeled by colloidal gold is distributed on the combined pad; and a qualitative line containing HCG monoclonal antibodies or goat-anti-mouse antibodies, a quality control line and a quantitative line are sequentially distributed on the analysis membrane at fixed points according to a chromatographic flow direction. The invention has the advantages that the qualitative detection can be realized, the sensitivity reaches 25IU/L, the quantitative function is obtained, the situation of barbs caused by too high HCG content of the existing detection test paper is overcome, the linearity range can reach 100-15000IU/L through providing the corresponding quantitative detector for detection under the situation that samples to be detected are not diluted, the inconvenience and the error caused by sample pre-dilution can be effectively avoided, the detection precision is high, the accuracy is high and the requirements on clinical examination are satisfied.

The invention discloses a novel SERS substrate-based method for quantitatively testing pathogenic bacteria, and belongs to the technical field of biological detection. Silicon dioxide treated by an immunospectral marker is coated with gold nanoparticles for quantitative test of the pathogenic bacteria. Raman spectra signal monitoring data are more stable and reliable and can be greatly repeated. The method is good in test stability, high in sensitivity, high in specificity, the detection process is simple, fast, accurate and quantitative, and a wide detection interval and a low detection limit are obtained. The method is simple in operation, an SERS immune signal probe and a capture probe can be prepared in advance, the capture probe and the signal probe only need to be sequentially added to a to-be-detected sample for reaction during operation and the test is carried out at once through magnetic separation after the reaction is completed: the collection time is 30s, and then the concentration is immediately read from a standard curve, thereby achieving fast quantitatively testing. The method can meet the requirements of food safety and environment monitoring departments, and has wide practicability.

The invention relates to a measuring method of smoldering speed of cigarette paper, which comprises a, preparing a cigarette hollow paper drum sample, b, setting relative marks, c, setting fixing method, d, setting test conditions, e, calculating out the cigarette paper smoldering speed via formula. Compared with prior three test methods, the invention has the advantages that 1, the test result has good repeatability, 2, the test result has good stability, 3, the test result can actually represent product quality, 4, the test result can represent the application character of product, 5, and the method has good operability. The test result indicates that since the cigarette paper has single-layer drum structure, is suspended and horizontally arranged and smoldered, the sample is in one small space, to make smoldering line regular, obtain the test result with good repeatability and reduce dispersion as small variation factor, thereby truly representing the quality and application character of product.

The invention provides a production method of carbon fiber filaments. The method includes the steps that firstly, a pre-oxidization furnace is adopted for performing pre-oxidization treatment on carbon fiber precursors according to a four-temperature-zone pre-oxidization process; secondly, a low-temperature carbonization furnace is adopted for performing low-temperature carbonization treatment according to a six-temperature-zone low-temperature carbonization process; thirdly, a high-temperature carbonization furnace is adopted for performing high-temperature carbonization treatment according to a four-temperature-zone high-temperature carbonization process; fourthly, sizing treatment is performed, and then the product is placed in a drying oven to be dried to obtain the carbon fiber filaments. By adjusting matching between the temperatures of pre-oxidization, low-temperature carbonization and high-temperature carbonization and the draft multiplication factor, the tension of the fibers can meet expected requirements in the operating process, the orientation degree of the fibers is high, molecular arrangement is more compact, and accordingly the strength of the carbon fibers is significantly improved.

The present invention is the method of detecting starch content in ash tree flower polysaccharide product. The detection includes the steps of eliminating liposoluble component, eliminating soluble saccharide as interference matter, enzyme decomposing starch, detecting starch, processing enzyme hydrolyzed starch liquid, Fehling's process of determining reducing sugar content, etc. The method ofthe present invention is suitable for operation, has stable and reliable determined result, small variation coefficient of determined result, less error, high repeatability and other advantages.

The invention provides a 6k polyacrylonitrile-based carbon fibre manufacturing method, which employs a routine method for obtaining a protofilament, and the carbon fiber is obtained by pre-oxidation, low temperature carbonization and high temperature carbonization of the protofilament. A spinning stock solution of the present invention employs a four-element formula, thereby improving the homogenization degree of the spinning stock solution in the solidification moulding process, and minimizing the performance variation coefficient of the protofilament. A copolymerization monomer is added during pre-oxidation, and reaction speeds of pre-oxidation and carbonization are increased, and the loop strength and modulus of carbon fiber are increased with smaller coefficient of variation by employing a low-carbon low-temperature and high draft ratio, and the tensile strength of the carbon fiber is substantially improved. In the low temperature and high temperature carbonization process, the matching of temperature and draft ratio is adjusted, thereby the tension force of the fiber in the operation process reaches the expected requirements that the low temperature carbonization tension force is 600CN and the high temperature carbonization tension force is 640CN, and the tension force of fiber in the operation process is increased, and the fiber orientation degree is improved, and the molecular arrangement is more dense, and the carbon fiber intensity is increased.

The invention relates to a newcastle disease virus/avian influenza virus H9 subtype/infectious bronchitis virus triplex fluorescence quantification detection reagent and a detection method and belongs to the technical field of animal quarantine. A newcastle disease virus M gene coding region specific sequence, an avian influenza virus H9 subtype H gene coding region specific sequence and a chicken infectious bronchitis virus M gene coding region specific sequence are selected as target regions, and on the basis of multi-sequence comparison, primer and probe design is conducted. The length of primers is about 20 basic groups, the GC content is 50-60%, a two-stage structure and repeatability do not exist in the primers, no complementary sequence exists between the primers or in the primers, and the melting temperature (Tm value) difference between the primers is smaller than 5 DEG C. In order to guarantee universal use of a newcastle disease virus probe, the length of the probe is only 13 basic groups, the probe is modified by LAN, and the Tm value of the probe is increased. The lengths of the other two virus probes are both about 25 basic groups, and the Tm values are about 5 DEG C higher than those of the primers.

The invention relates to a kit for rapidly diagnosing lipoprotein-associated phospholipase A2 and a use method of the kit. The kit comprises a standard substance, a quality control substance, a liposome complex, a magnetic separation reagent, an assay buffer solution and a cleaning solution, wherein the inner part of the liposome complex is coated with N-hydroxysuccinimide ester of tris (bipyridine) ruthenium; an antibody for resisting the lipoprotein-associated phospholipase A2 is connected to the surface of the liposome complex by interaction of streptavidin and biotin; the magnetic separation reagent takes magnetic particulates containing amino terminals as a carrier and is connected with the antibody for resisting the lipoprotein-associated phospholipase A2 through the interaction of the streptavidin and the biotin. Compared with an enzyme linked immunosorbent assay kit produced in a foreign Diadexus company, the kit prepared by the invention has the advantages that sensitivity and precision for analyzing the lipoprotein-associated phospholipase A2 are greatly improved; in addition, the kit disclosed by the invention is simple and convenient to operate, quick and easier to popularize.

The invention belongs to the technical field of biology and relates to an imidaclothiz antibody specifically-bound polypeptide and use of the polypeptide in detection of imidaclothiz. According to the use, screening of three times is carried out on a random cyclo-heptapeptide library, a cyclo-octopeptide library and a linear-dodecapeptide library for phage display by using a protein A column purified imidaclothiz antibody by adopting a phage display technology, so as to screen out phage clones bound to the imidaclothiz antibody; and amplification and plasmid extraction are carried out on a plurality of randomly-selected phage clones, positive phage clones are selected by phage ELISA (Enzyme-Linked Immunosorbent Assay), and sequencing is carried out on the positive clones so as to obtain a polypeptide sequence. The invention further relates to the use of the phage display polypeptide in the detection of the imidaclothiz. The phage ELISA established by using the phage display polypeptide can be used for detecting imidaclothiz residues in surroundings and agricultural products in a rapid, sensitive, simple, convenient and cheap manner.

The invention is applicable to the field of pharmaceutic preparations, and provides an S-pantoprazole sodium enteric-coated tablet and a preparation method thereof. The enteric-coated tablet is prepared by wrapping an S-pantoprazole sodium tablet core with an isolated layer and an enteric layer; the tablet core comprises the following components by weight percent: 15-20% of S-pantoprazole sodium, 60-75% of filler, 0.2-1% of binder, 2-8% of disintegrant, 4-10% of stabilizer and 0.2-1% of lubricating agent, wherein the weight gain of the isolated layer is 1-10% of weight of the tablet core; the weight gain of the enteric layer is 8-18% of the weight of the tablet core. By adopting the S-pantoprazole sodium enteric-coated tablet provided by the invention, not only can the problem of an unstable storage process be solved, but also the drug disintegration time can be shortened, and the problem of a large digestion variable coefficient is improved.

The invention relates to a multi-level tobacco leaf module threshing and re-baking feeding processing method, and belongs to the technical field of threshing and re-baking production. The object of the method is to solve the problems that after the processing of a threshing and re-baking conventional feeding processing mode of a multi-level tobacco leaf module formula, the variation coefficient offinished strip nicotine is large, and the processing mode of overhead storage feeding is limited by the number of tobacco leaf levels in the multi-level tobacco leaf module formula; the method is applicable to the multi-level tobacco leaf module formula composed of multi-level tobacco leaves with a number of more than 20 levels, according to chemical compositions and the module formula of single-level tobacco leaves in a formula module, and secondary compounding is conducted on the multi-level tobacco leaf module formula, so that the content of nicotine in each layer of tobacco leaves entering a pre-matching cabinet is even and stable; the method can be cooperated with threshing re-baking conventional feeding and overhead storage feeding modes to achieve the fine processing of the nicotine in the threshing and re-baking feeding tobacco leaves, the nicotine variation coefficient of the threshing and re-baking finished strips is greatly reduced, and the homogenization processing of thenicotine of the feeding tobacco leaves can be achieved.

The invention discloses a set of sulfamethazine specifically-bound aptamers and a screening method and applications thereof. The aptamer for identifying sulfamethazine, disclosed by the invention, is a single-stranded DNA molecule shown in any one of formulas (1)-(4): (1) a single-stranded DNA molecule shown in a sequence 3 in a sequence table; (2) a single-stranded DNA molecule shown in a sequence 1 in the sequence table; (3) a single-stranded DNA molecule shown in a sequence 2 in the sequence table; and (4) a single-stranded DNA molecule shown in a sequence 4 in the sequence table. Experiments show that a directly competitive chemiluminescence analysis method established based on biotinylated aptamers and used for detecting sulfamethazine provides a basis for the practical application of the aptamers of sulfamethazine, and provides technical support for more efficient and extensive monitoring of antimicrobial drug residues.

The invention discloses a method for detecting olaquindox by a directly-competing TRFIA (Time Resolved Fluorescence Immunoassay). The method comprises the following steps of: respectively adding standard olaquindox solution or treated sample solution and europium-labeled monoclonal antibodies into each micropore of an antigen precoating strip, mixing to be uniform, incubating for 1 hour at the temperature of 37 DEG C, and washing the micropores for 3-5 times by using washing liquid; adding 200muL of enhancement solution into each micropore, and carrying out light-proof oscillating incubation for 10 minutes at the temperature of 37 DEG C; determining the fluorescence intensity value cps by using a time resolution meter; drawing a standard curve; calculating out the corresponding concentration of the olaquindox from the standard curve according to the cpsx/cps0 value of each sample, multiplying by the corresponding dilution ratio and calculating out the actual concentration of the olaquindox in the sample. The method disclosed by the invention has the advantages that the operation is simple and convenient, the sensitivity is high, the stability is good and the lowest detection limit can reach 0.83ng.mL<-1>.

The invention discloses a medical ultrasound image speckle removing method through quantum inspiration. The method comprises the steps that a medical ultrasound image containing speckle noise is input; logarithm transformation is carried out to convert the multiplicative noise image into the additive noise image; complex wavelet transformation is carried out to convert a grey value of the image into a wavelet coefficient; noise variance, and variance and smoothing parameters of a probability density function of an ideal image signal are estimated to obtain a noise statistic model and a statistic model of the ideal image signal; an adaptive adjustment threshold value is calculated according to the theory of quantum inspiration, and soft threshold processing is carried out on the wavelet coefficient to obtain a wavelet coefficient estimation value of the ideal image signal; the wavelet coefficient estimation value of the ideal image signal is used for wavelet reconstruction to obtain the image; exponential transformation is carried out on the image to compensate the logarithm transformation in the first step, and the image with speckles removed is obtained. The method can keep organization details in the image well on the basis of effectively removing the speckle noise of the medical ultrasound image, and play a good role in assisting in medical treatment.

The invention discloses a method for detecting the residual quantity of ivermectin in sheep muscle tissues by using a liquid chromatograph/mass spectrometer with doramectin as an internal standard substance. The method comprises the following steps: extracting ivermectin residual in the sheep muscle tissues with acetonitrile as an extraction solvent, adopting an impurity adsorption and solid phase extraction mode, adopting an alkaline alumina column as a solid phase extraction column, introducing acetonitrile, eluting, and collecting all obtained eluate. The method solves the problems of complex extraction and purification processes and high detection limit of traditional detection methods, realizes enrichment and purification of ivermectin residual in the sheep muscle tissues, improves the detection sensitivity, repeatability and accuracy, and is suitable for batch sample detection.

The invention relates to cannabidiol compositions and application thereof. Specifically, the invention relates to a cannabidiol composition containing cannabidiol and a surfactant. The composition cansignificantly improve the water solubility of cannabidiol to make cannabidiol to be better applied in the fields of medicine, daily chemical, food, health products and the like.

The invention discloses a preparation method of heat-not-burn dry reconstituted tobacco. The preparation method comprises the steps of substrate preparation, coating and drying, and further comprisesthe step of drying and calendering treatment. By adjusting the proper calendering pressure and calendering temperature, the physical properties of the heat-not-burn dry reconstituted tobacco are improved, and the processing requirements of the forming process are met; the deformation coefficient of a formed material rod is small, a smoke producing agent is formed by adding coating liquid to be combined with added glycerol, and the drying temperature is adjusted so that it can be guaranteed that the content of glycerol in a product can reach the standard, and processing adaptability indexes such as tensile strength of the heat-not-burn dry reconstituted tobacco can also be met. The smoke producing agent content of the prepared dry reconstituted tobacco can reach 19.53%, the tensile strengthof reconstituted tobacco before calendering is 0.315 KN/m, the tensile elongation of reconstituted tobacco before calendering is 6.05%, the tensile strength of the dry reconstituted tobacco after calendering reaches 0.5337 KN/m, and the tensile elongation of the dry reconstituted tobacco after calendering is 11.13%.

The invention belongs to the field of food, and relates to a method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat. The method comprises the steps of carrying out a full homogenate treatment on a sample, then centrifuging the obtained homogenate and taking supernatant; reading by using a handheld ATP fluorescence detector; converting the reading number into a mole number of n ATP according to a standard curve fitted in advance, and further calculating the n ATP. Compared with other methods for measuring the content of ATP in the meat, the method provided by the invention is simpler, rapider, more reliable and less in variable coefficient. The method can be used for evaluating the freshness and shelf life of the commercial available iced chicken meat products.

The invention relates to a method for detecting phytase activity in a feed and belongs to the technical field of chemical composition analysis of feeds. In the method, phytase in the feed is completely leached through novel extract and diluent and a detection flow, the interference of inorganic phosphorus present in the feed on the detection is eliminated, the coefficient of variation of the detected result is small, and the method has the advantages of short detection period, high sensitivity, high repeatability and high accuracy.

The invention discloses an in vitro biomimetic digestion method for rapidly measuring the pig feedstuff protein digestibility, comprising the following steps: pulverizing feedstuff samples and the pulverized feedstuff samples passing through a standard sieve; preparing gastric buffer liquid and intestinal buffer liquid; preparing simulated gastric juice, concentrated simulated intestinal juice, concentrated supplementing intestinal juice; filling the gastric buffer liquid and the intestinal buffer liquid into an insulating chamber for the biomimetic digestion of monogastric animals, and connecting a pipeline communicating with a simulated digester; loading the pulverized feedstuff samples into the simulated digester, and then adding the simulated gastric juice, and putting into the biomimetic digestive system of monogastric animals. The in vitro biomimetic digestion process of the pig feedstuff protein is automatically completed by setting up a control software of the biomimetic digestive system for biomimetic digestion parameters at each stage of the stomach and the intestine; after obtaining the undigested residue, the crude protein content of the residue and the feedstuff samples is determined after drying, and the protein digestibility is calculated. The method is simple, high in precision and low in implementation cost, and can accurately measure the protein digestibilityof pig feedstuff within 48 hours.