Method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat

A chilled and chicken technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of no standard method, inaccurate results, and ATP bioluminescence technology has not yet been found, and achieve the effect of small coefficient of variation

Inactive Publication Date: 2016-06-22
YANGZHOU UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, this method has been used to detect the number of microorganisms on the surface, and there are problems such as large coefficient of variation and inaccurate results.
So far, no ATP bioluminescent technology has been found for the detection of ATP content in meat tissue, let alone a unified standard method

Method used

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  • Method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat
  • Method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat
  • Method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Material: chilled chicken.

[0020] Reagents: ATP disodium salt hydrate, phosphate buffer (1X), sterile water, ATP detection swab.

[0021] Instruments: handheld ATP fluorescence detector, Precelly24 homogenizer.

[0022] (1) ATP standard curve

[0023] ATP standard sample dilution: Dilute the ATP standard sample solution step by step to 10 with sterilized water -6 g / ml, 10 -7 g / ml, 10 -8 g / ml, 10 -9 g / ml, 10 -10 g / ml. Take a sample of 10 μl, then the moles of ATP are 0.2×10 -11 mol, 0.2×10 -12 mol, 0.2×10 -13 mol, 0.2×10 -14 mol, 0.2×10 -15 mol.

[0024] 1.2.2 Determination of ATP standard dilution sample Take 10 μl of ATP dilution solution with different gradients, put it on the ATP swab cotton swab, put on the coat, squeeze the reaction solution on it, react for 10 seconds, then put it into the portable ATP detector, and read after 10 seconds. Each sample was measured 3 times.

[0025] Make a standard curve according to the assay results of ATP standard...

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Abstract

The invention belongs to the field of food, and relates to a method for rapidly detecting adenosine triphosphate (ATP) in iced chicken meat. The method comprises the steps of carrying out a full homogenate treatment on a sample, then centrifuging the obtained homogenate and taking supernatant; reading by using a handheld ATP fluorescence detector; converting the reading number into a mole number of n ATP according to a standard curve fitted in advance, and further calculating the n ATP. Compared with other methods for measuring the content of ATP in the meat, the method provided by the invention is simpler, rapider, more reliable and less in variable coefficient. The method can be used for evaluating the freshness and shelf life of the commercial available iced chicken meat products.

Description

technical field [0001] The invention is applicable to the field of food. It involves a new method for rapid detection of ATP in chilled chicken. Background technique [0002] ATP (adenosinetriphosphate), adenosine triphosphate, is considered to be a universal energy source essential in cellular synthesis for the survival and reproduction of all organisms, and it is a universal energy "currency". Since Lohmann first discovered and extracted ATP from muscle with high sugar catabolism in 1929, it has been confirmed to play an extremely important role in the metabolism of living cells and the energy supply of various biochemical reactions. However, after the animal body dies, ATP mainly comes from glycolysis, with less synthesis and more metabolic consumption, resulting in continuous degradation of ATP until it disappears. By using the ATP content in muscle tissue at different times after chicken slaughter, the time of initial slaughter and processing can be judged, so as to i...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 常国斌鲁伟仇玲玲张扬陈国宏徐琪袁青妍张康宁许盛海
Owner YANGZHOU UNIV
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