563 results about "High sugar" patented technology

Various embodiments of freeze dryers and drying processes are disclosed that can be used to dry materials with low water content (no more than 20 wt % water) and / or high sugar content (at least 20 wt % sugar). Such materials include candy, confections, and the like. In one embodiment, a drying process includes drying the materials without freezing them. In another embodiment, a drying process includes warming the materials before drying them. In another embodiment, a drying process includes drying two batches of materials with low water content sequentially without defrosting the interior of freeze dryer.

The invention discloses a Pichia kluyveri yeast and its application. The Pichia kluyveri yeast is classified and named as Pichia kluyveri JLG30201, which has been deposited in the China Center for Type Culture Collection, with a deposit number of CCTCC NO: M 20242295 and a deposit date of October 22, 2024. The Pichia kluyveri JLG30201 has strong killer affinity and high sugar tolerance, unique aroma production, high production of extracellular superoxide dismutase (SOD), and low alcohol production; the strain is applied to the processing of fruit wine or edible plant enzymes, and has the effects of increasing ester and flavor, improving juice yield and product antioxidant activity.

The invention relates to application of fermentation lactobacillus mucus LFPerfectuus001 in preparation of a product for improving metabolic syndrome caused by high-fat and high-sugar diet, and belongs to the technical field of microorganisms. The invention provides application of fermented lactobacillus mucus LFPerfecus001 in preparation of a product for improving metabolic syndrome, the fermented lactobacillus mucus LFPerfecus001 is preserved in Guangdong Microbial Culture Collection Center on April 13, 2022, and the preservation number is GDMCC No: 62335. The invention further provides a preparation method of the fermented lactobacillus mucus LFPerfecus001. Animal experiments report that the fermented lactobacillus mucus LFPerfectus001 has the effects of reducing fat, increasing muscle, improving obesity, hyperglycemia, insulin resistance, dyslipidemia, hyperuricemia and fatty liver for the first time, and can well improve metabolic syndrome caused by high fat and high glucose.

The invention relates to a solid beverage containing mulberry leaf and emblic leafflower fruit components. The solid beverage comprises the following components: a mulberry leaf-emblic leafflower fruit fermentation extract, collagen tripeptide, fructo-oligosaccharide, vitamin C, zinc gluconate, vitamin E, vitamin B6 and vitamin B2. The solid beverage disclosed by the invention contains components such as mulberry leaf and phyllanthus emblica collagen tripeptide, and particularly contains a mulberry leaf and phyllanthus emblica mixed fermentation extract obtained by an original fermentation process, so that contained anti-sugar and anti-oxidation components are stimulated, the generation of free sugar can be greatly inhibited, and oxygen free radicals can be eliminated; the product can be used as a whitening food for alleviating the problems of dull yellow facial skin, coarse pores and the like caused by high-sugar diet, so that the skin is whitened and fine.

The invention relates to the field of microbial fermentation, in particular to an ethyl carbamate-reducing Caribik Yarrowia spp. And application of the Caribik Yarrowia spp. The invention discloses Meyerozyma caribba P3, the enzyme activity of ethyl carbamate hydrolase of the Meyerozyma caribba P3 is high and can reach 980.40 U, and when the Meyerozyma caribba P3 is applied to yellow wine fermentation, 49.5% of yellow wine EC can be degraded. The yeast strain for degrading the ethyl carbamate in the fermented food has the advantages of being high in fermentation capacity, capable of tolerating low-acid, high-sugar and 12% alcohol environments and the like, and a valuable strain source is provided for degrading the ethyl carbamate in the fermented food through a microbiological method.

The invention relates to application of sea cucumber-derived nano-vesicles in preparation of a medicine for promoting wound repair. The nano-vesicles (SC-EVs) extracted and prepared from sea cucumbers can effectively promote cell migration, scratch repair and tube forming capabilities of human skin epithelial cells (HACAT) and human skin microvascular endothelial cells (HM DEC) and inhibit cell apoptosis, so that the capability of repairing cells is achieved, and particularly the effect of repairing wound surfaces in a high glucose state or diabetes mellitus is achieved. The invention provides a new direction and thought for the application of the sea cucumber-sourced nano vesicles (SC-EVs), provides a brand new drug research and development and clinical treatment direction for wound repair, especially for solving the problem of difficult healing of wounds of patients with high glucose or diabetes, and has extremely high social value and market application prospect.

The invention belongs to the technical field of biological medicines, and particularly relates to an application of a Pucurbita pepo polypeptide Nacrein-LP20 in preparation of a medicine for promoting wound healing and a preparation method of the Pucurbita pepo polypeptide Nacrein-LP20. The amino acid sequence of the Hepu pearl polypeptide Nacrein-LP20 is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). According to the present invention, the Pucurbita pepo active polypeptide Nacrein-LP20 has characteristics of strong DPPH free radical and hydroxyl free radical removing ability and excellent antioxidant activity, can significantly promote HaCaT cell proliferation in the high glucose environment, and can promote migration and repair of HaCaT cells in the high glucose environment; the traditional Chinese medicine composition has a remarkable healing promoting effect on full-cortical resection wound surfaces of normal rats and diabetic rats.

The invention relates to the technical field of artificial intelligence, and discloses a blood glucose management system based on artificial intelligence. According to the system, blood glucose monitoring is reminded through the blood glucose monitoring terminal, a blood glucose curve is drawn every week, abnormal blood glucose conditions are judged for reminding and alarming, the diet terminal is used for guiding low-sugar diet recommendation, high-sugar food intake is avoided, the mastering degree of a patient and family members for food classification is improved, and the exercise terminal determines the exercise time and exercise amount of the patient according to the blood glucose value. Parameters are input into an intelligent system, the system converts and selects a motion mode, time and intensity, a drug treatment terminal calculates the insulin injection amount of a patient every day and every meal according to the blood glucose level and food intake, the insulin dosage is adjusted according to the calculated numerical values, blood glucose fluctuation is reduced, and the treatment effect is improved. The education and self-management terminal guides a patient to improve the cognitive degree of diabetes and provides personalized propaganda and education guidance for the patient, and the complication reminding terminal controls the blood sugar of the patient within a normal range, guides a good lifestyle of the patient and guides behavior specifications.

The invention relates to the technical field of food processing machinery, in particular to a double-shaft stirring dough kneading device for high-oil and high-sugar dough, which comprises a stirring tank main body, a tank cover is arranged at the top of the stirring tank main body, and a main stirring shaft and an auxiliary stirring shaft which are positioned in an inner cavity of the stirring tank main body are respectively arranged on two sides of the lower surface of the tank cover; wave-shaped blades are evenly distributed on the outer side of the main stirring shaft, fan-shaped blades are evenly distributed on the outer side of the auxiliary stirring shaft, synchronous driving mechanisms corresponding to the main stirring shaft and the auxiliary stirring shaft are arranged on the upper surface of the tank cover, and a temperature control mechanism is arranged at the bottom of the stirring tank body. The double-shaft staggered reverse differential stirring device has the beneficial effects that through double-shaft staggered reverse differential stirring, a grease wrapping layer can be quickly and effectively broken, sugar oil is promoted to be uniformly dispersed and uniformly distributed and fully blended into dough, and then grease and sugar are fully blended into the dough, so that the dough is compact in structure, and the stirring and dough kneading effects are relatively good.

The invention relates to the technical field of food nutrition and health care and biological medicines, in particular to application of beta-1, 3 / alpha-1, 3-glucan in preparation of medicines for treating and / or preventing obesity and / or body weight super-severe illness and digestion-aiding health food. According to the experimental results, high-glucose and high-fat rats are continuously fed in the stomach for 8 weeks, and the food intake can be effectively reduced and the body weight can be reduced through preventive administration; the conclusion that beta-1, 3 / alpha-1, 3-glucan has the digestion-aiding health-care food function can be obtained according to the requirements of'health-care food function inspection and evaluation method (2023 edition) by combining the research results that beta-1, 3 / alpha-1, 3-glucan has the stimulation effect on small intestine peristalsis, promotes the growth of intestinal beneficial bacteria and regulates intestinal health in the existing research. The invention provides a new direction for preparing the medicine for treating and / or preventing obesity and / or body weight super-severe illness and health-care food beneficial to digestion.

The invention discloses a fruit and vegetable extract extraction method combined with ultrasonic enhancement, and particularly relates to the field of fruit and vegetable extract extraction.The fruit and vegetable extract extraction method comprises the steps that a composite micelle structure formed by saccharides and functional factors in fruit and vegetable raw materials is recognized as a structure obstacle before extraction, and a directional unbinding path is constructed according to the binding configuration and structural responsiveness of the structure obstacle; to generate a group of ultrasonically extracted target components; and constructing an ultrasonic cooperative transmission path with continuous penetrability and cavitation energy focusing capability by taking the sound response condition and the foam interference characteristic of the liquid phase environment in which the target component group is located as dynamic parameters. By constructing a multi-dimensional synergistic extraction process of a sugar-phenol deconstruction path, a cavitation energy penetration path and a migration enrichment feedback path, the problems of low functional component release efficiency and difficult purity control caused by superposition of a wrapping effect and an acoustic resistance barrier in a high-sugar system are solved.

The invention discloses a functional feed formula for improving endurance and reproductive performance of donkeys, and relates to the technical field of feed formulae, the functional feed formula comprises selection and pretreatment of raw medicinal materials, nutrient element proportioning, treatment of functional reinforcing materials and treatment after molding, the functional reinforcing materials comprise a functional formula A and a functional formula B, the method has the advantages that the mixed leaves are subjected to low-temperature freeze-drying treatment and then crushed into powder, bioactive components such as protein and flavonoids in the leaves are reserved, the leaves can be used as a high-quality plant protein source and a functional component source, carbon dioxide gas is introduced into the compound protein leaf powder for activation, and the compound protein leaf powder can be used as a high-quality plant protein source and a functional component source. The method has the advantages that bioactive components in the leaf powder can be activated, the bioavailability of the leaf powder can be increased, polysaccharide in the high-sugar Chinese velvet herb can be further decomposed into monosaccharide and oligosaccharide by saccharifying the high-sugar Chinese velvet herb, and the energy release speed can be increased.

The invention discloses a composition for assisting in reducing blood sugar and blood fat as well as a preparation method and application thereof, and relates to the technical field of functional compositions. The composition comprises highland barley powder and modified inulin, the preparation method of the highland barley powder comprises the following steps: soaking highland barley grains in a weakly alkaline aqueous solution, and sequentially carrying out first pulse microwave treatment, enzyme deactivation treatment and segmented baking treatment; the segmented baking treatment comprises first-stage baking treatment and second-stage intermittent infrared irradiation treatment; the temperature of the first-stage baking treatment is 40-60 DEG C; the temperature of the second-stage intermittent infrared irradiation treatment is 60-80 DEG C, and the emission wavelength is 2.5-4 microns; the preparation method of the modified inulin comprises the following steps: performing acidolysis treatment on inulin in a weakly acidic aqueous solution, and then drying. The highland barley powder and the modified inulin in the embodiment can synergistically improve glycometabolism and lipid metabolism, effectively reduce the body weight, blood sugar, blood fat and the like under high-glucose and / or high-fat diet, and protect and repair the pancreas islet function.

The invention discloses a non-invasive hyperglycemia detection method based on a multi-modal physiological signal, and the method comprises the steps: obtaining the multi-modal physiological signal of a person to be detected, the multi-modal physiological signal comprising a BVP signal, an HR signal and an EDA signal; extracting time domain features and frequency domain features of the BVP signal, the HR signal and the EDA signal through three-stage discrete wavelet transform, extracting DNN features of the BVP signal, the HR signal and the EDA signal through a preset CNN-LSTM network, and fusing the time domain features, the frequency domain features and the DNN features by using a deep neural network to obtain a mixed feature vector; and inputting the time domain features, the frequency domain features, the DNN features and the mixed feature vectors of the BVP signal, the HR signal and the EDA signal into a pre-trained integrated learning model, and predicting whether the to-be-detected person is in a high glucose state. The method has the advantages of noninvasiveness, high precision, real-time performance and individuation, is suitable for continuous monitoring and early screening of prediabetic people, and has good clinical prospects and practical application values.

The invention provides a mulberry leaf extract rich in sugar inhibition components and a preparation method and application thereof, and the method comprises the following steps: S1, after mulberry leaves are subjected to water extraction, collecting a first water extract and water extraction residues; s2, fermenting the water extraction residues, performing water extraction, and collecting a second water extraction solution; s3, mixing the first water extract and the second water extract, and then performing flocculation, microfiltration, ultrafiltration, membrane concentration and drying; a flocculating agent in the flocculation process is modified chitosan; the modified chitosan is prepared from the following raw materials in parts by weight: 10 parts of chitosan, 1-2 parts of aldehyde sucrose and 3-4 parts of amino polyethylene glycol. According to the preparation method, the steps of the preparation process are controlled, so that the mulberry leaf extract with high sugar inhibition component content is prepared.

This invention discloses a method for preparing a pH / glucose dual-responsive chronic wound dressing containing frankincense essential oil, comprising: (1) using 4-formaldehyde phenylboronic acid as a crosslinking agent to form Schiff base and borate ester bonds with carboxymethyl chitosan and two different molecular weight hyaluronic acids, respectively, to prepare a hydrogel capable of filling skin surface wounds and performing dual dynamic covalent crosslinking adhesion, and freeze-drying for later use; (2) preparing frankincense essential oil into microcapsules by emulsification coagulation method, and freeze-drying for later use; (3) mixing the hydrogel with the freeze-dried powder of frankincense essential oil microcapsules, adding deionized water or PBS and stirring to reconstitute to form a pH / glucose dual-responsive hybrid hydrogel dressing coated with frankincense essential oil microcapsules. The composite dressing prepared by this invention has anti-inflammatory, antioxidant, antibacterial, and healing-promoting effects, is quick and easy to prepare, and has the ability to respond to the acidic / high-glucose microenvironment of diabetic wounds, and has important practical application value.

The invention relates to the technical field of molecular biology, gene therapy and drug development, and provides application of TRIM44 as a target in preparation of a drug for treating non-alcoholic fatty liver. The medicine prevents, relieves and / or treats the non-alcoholic fatty liver disease by inhibiting the expression level of TRIM44. According to the application disclosed by the invention, the TRIM44 is taken as a target, and the non-alcoholic fatty liver is prevented, relieved and treated by reducing the expression level of the TRIM44. The TRIM44 inhibitor enables the liver weight and the proportion of the liver in the body weight of a patient to be remarkably reduced, lipid accumulation to be reduced, fat deposition and vacuolation degree of liver tissue to be reduced and liver texture to be improved under poor diet conditions of high fat, high sugar and the like, so that the non-alcoholic fatty liver is prevented, relieved and treated, and the health state of the patient is improved.

The present invention discloses the application of ginseng exosomes in the preparation of nanomedicines for treating diabetic ulcer vascular lesions, and relates to the field of biomedicine. The present invention verifies through experiments that ginseng exosomes can reprogram the glycolysis process of vascular endothelial cells under high sugar conditions, and inhibit the expression of acetyl coenzyme A in cells, reduce the production of reactive oxygen species, and then promote the expression of nitric oxide synthase, thereby reducing or reversing cell apoptosis induced by high sugar. Ginseng exosomes are used as nanomedicines to prevent, treat or improve diabetic ulcer microangiopathy. The ginseng exosomes in the present invention have a simple preparation process, low cytotoxicity, high delivery efficiency, and good stability. They can be used as a new type of nanomedicine for the prevention, treatment or improvement of diseases related to diabetic vascular lesions.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention relates to the technical field of biological pharmacy, in particular to application of a recombinant protein DEL-1 in preparation of a medicine for resisting pancreatic beta cell inflammation. The recombinant protein DEL-1 has a remarkable inhibition effect on pancreatic beta cell inflammatory response induced by high glucose and high fat. Experiments show that in an islet beta cell inflammation model constructed by combining high glucose with palmitic acid, DEL-1 can significantly reduce the expression level of inflammatory factors such as IL-1beta, IL-6, TNF-alpha and the like. The DEL-1 is proved to have a definite effect of resisting pancreatic beta cell inflammation.

The present invention discloses a low - serum medium and its application in the preparation of cultured meat from Larimichthys crocea cells, which is also applicable to other marine or freshwater fish, belonging to the field of bioengineering technology. The low - serum medium comprises a basal medium, fetal bovine serum accounting for 30 - 80 μL / mL, and exogenous additives. The exogenous additives include human serum albumin, ascorbic acid, basic fibroblast growth factor polypeptide, human holo - transferrin, lysophosphatidic acid, and human recombinant IGF - 1. Based on the DMEM / F12 medium, the low - serum medium provided by the present invention adds six substances that promote cell growth, reduces the dependence of satellite cells on fetal bovine serum, effectively reduces the usage amount of fetal bovine serum, and saves the culture cost. Compared with the general - type medium (DMEM high - glucose, adding 10% FBS), the low - serum medium provided by the present invention has an equivalent effect on the maintenance and proliferation ability of cell stem cells.

The present invention belongs to the technical field of organic synthesis, and specifically relates to loganin derivatives and preparation methods thereof and applications in the preparation of drugs for preventing or treating diabetes and diabetic complications. The loganin derivatives provided by the present invention are derived from the natural product loganin, and have a structure shown in formula I, formula II-1 or formula II-2. Compared with loganin with great polarity, it has low polarity and good fat solubility, which is conducive to its absorption in the body. The loganin derivatives provided by the present invention have a significant inhibitory effect on the abnormal proliferation of HBZY-1 cells induced by high sugar at different concentrations; the SOD activity in HBZY-1 cells can be significantly increased and the content of MDA can be reduced. The loganin derivatives provided by the present invention have the characteristics of high efficiency and low toxicity. Therefore, the loganin derivatives with a structure shown in formula I or formula II provided by the present invention can be used in the preparation of drugs for preventing or treating diabetes and diabetic complications.

The invention discloses a construction method of a metabolism-related fatty liver cancer (MAFLD liver cancer) mouse model, and relates to the technical field of biology. The specific construction method comprises the following steps: feeding a 7-9-week-old mouse with a high-fat and high-sugar diet for 24 weeks, inducing the mouse to form fatty liver, and causing metabolic dysfunction; injecting carbon tetrachloride into the abdominal cavity of the mouse once a week while feeding the mouse with the high-fat and high-sugar diet, and inducing the liver of the mouse to form liver cancer continuously for 24 weeks, so as to construct the MAFLD liver cancer mouse. The mouse liver constructed by the method has metabolic dysfunction, fatty liver and liver cancer lesions, and has extremely high application value in research and development of drugs for researching MAFLD liver cancer pathogenesis and fatty liver related liver cancer in the future.

The invention provides a bismuth-based heterogeneous material for promoting healing of diabetic wounds and a preparation method and application thereof. The bismuth-based heterogeneous material comprises a bismuth-based porphyrin metal organic framework, and the surface of the bismuth-based porphyrin metal organic framework is modified with Au-Pt nanoparticles. According to the bismuth-based heterogeneous material disclosed by the invention, microenvironments of bacterial infection, high glucose, active oxygen damage, inflammatory disorder, pH rise and oxygen deficit of diabetic wounds are improved through multiple antibacterial effects and enzyme catalysis effects, so that the healing of the diabetic wounds is promoted.

The invention discloses a preparation method of a pH / glucose double-response chronic wound dressing containing frankincense essential oil, which comprises the following steps: (1) respectively forming Schiff base and boric acid ester bonds with carboxymethyl chitosan and two hyaluronic acids with different molecular weights by using 4-formylphenylboronic acid as a cross-linking agent; preparing adhesive hydrogel which can be used for skin surface wound surface filling and double-dynamic covalent cross-linking, and freeze-drying for later use; (2) preparing the frankincense essential oil into microcapsules by an emulsification and condensation method, and freeze-drying for later use; and (3) mixing the hydrogel with the freeze-dried powder of the frankincense essential oil microcapsules, adding deionized water or PBS, stirring and redissolving to form the pH / glucose double-response hybrid hydrogel dressing coated with the frankincense essential oil microcapsules. The composite dressing prepared by the invention has the effects of resisting inflammation, resisting oxidation, resisting bacteria and promoting healing, is quick and simple to prepare, has stimulation response capability on acid / high-glucose microenvironment of diabetic wound surfaces, and has important practical application value.

The invention discloses a construction method and application of a metabolic disorder related fatty liver disease damp-heat syndrome mouse model, and relates to the technical field of biological model construction.The construction method is technically characterized in that the damp-heat environment is simulated through a simple and convenient method of combining a silica gel heating plate with wet padding, and high-fat and high-sugar feed feeding is combined, so that the metabolic disorder related fatty liver disease damp-heat syndrome mouse model is obtained. The MASLD damp-heat syndrome mouse model is stably induced, and the equipment acquisition difficulty and the modeling cost in the research process are reduced; the mouse model is used for research on construction of MASLD of various symptom types mainly including damp-heat.

The invention relates to saccharomyces cerevisiae and application thereof in wine brewing. The saccharomyces cerevisiae strain is saccharomyces cerevisiae Y24 and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on March 10, 2025, and the preservation number of the saccharomyces cerevisiae strain is CGMCC (China General Microbiological Culture Collection Center) NO. 33773. The saccharomyces cerevisiae strain is a saccharomyces cerevisiae strain. The saccharomyces cerevisiae Y24 strain can grow under the conditions that the alcoholic strength is 18% vol, the pH value is 2, the sugar content is 60% and the temperature is 4 DEG C, has good high-alcoholic strength, high-acid and high-sugar endurance capacity, and meets the low-temperature fermentation requirement of wine. Meanwhile, in wine fermented by the saccharomyces cerevisiae Y24 strain, the content of dry extracts capable of enriching wine bodies is higher, the content of aroma components is higher, and the content of undesirable component methanol is lower.

The invention discloses a low-temperature hot air drying preparation process of low-sugar preserved mangoes. The preparation process comprises the following steps: S1, pretreating mangoes; s2, blanching is conducted; s3, hardening and color protection; s4, rinsing is carried out; s5, performing sugaring; s6, sugar draining; s7, drying; and S8, cooling. The sensory score of the product prepared by the process is 86.96, and the product is golden in color, rich in fragrance and palatable in sour and sweet taste. The total sugar content is 35.88% and is obviously lower than that of the traditional high-sugar preserved fruit. And the water content is 21.78%. The hardness is 3216.35 + / -g, the cohesiveness is 0.88, the chewiness is 3155.35, the resilience is 0.56, and the texture property is good. The total bacterial colony number 1t; 10 CFU / g, and coliform bacteria are not detected.

The invention belongs to the technical field of biological medicine, and particularly relates to application of CNPY2 as a new target in diagnosis and treatment of diabetic nephropathy. The invention provides a biomarker CNPY2 gene or CNPY2 protein for auxiliary diagnosis of diabetic nephropathy for the first time. The expression of the CNPY2 in the renal tubule of a DN patient is up-regulated, the expression of the CNPY2 in the renal tubule of a db / db mouse is up-regulated, and the expression of the CNPY2 in an HK-2 cell subjected to in-vitro high-glucose culture is up-regulated; therefore, the CNPY2 can be used as an auxiliary diagnosis index of the diabetic nephropathy for auxiliary diagnosis of the diabetic nephropathy. The invention also provides a kit for auxiliary diagnosis of diabetic nephropathy. The kit contains a detection reagent of the CNPY2 gene or the coded protein. The invention also finds that by knocking down the adeno-associated virus through the CNPY2 and inhibiting the expression or activity of the CNPY2 through the specific small interfering RNA of the adeno-associated virus, the pathological injury of the renal tubules of db / db mice can be obviously improved, the integrity of MAM in the renal tubule epithelial cells is improved, and the ferroptosis, ERS and mitochondrial injury of the renal tubule epithelial cells are alleviated; therefore, the CNPY2 can be used as a drug, a drug target or a target gene in gene therapy, and is applied to prevention, alleviation or / and treatment of diabetic nephropathy.

The invention discloses an application of a Solyc02g060560 gene in regulation and control of sugar content and size of tomato fruits, wherein a CDS (Coding Sequence) sequence of the Solyc02g060560 gene is as shown in SEQ ID NO. 1. A tomato transcription factor mutant library is constructed through a CRISPR (clustered regularly interspaced short palindromic repeats) technology, and a mutant TOM6-126 with unknown functions at present in a C2H2 family is screened. Based on observation of the phenotype of the mutant, the inventor determines that the gene is related to the sugar content and the fruit size of tomato fruits. Compared with a wild type, the soluble solid content (brix) of tomato fruits of the mutant is increased, and the fruits become larger. It is indicated that the Solyc02g060560 gene plays an important role in negative regulation of tomato fruit growth and development and sugar content. According to the invention, a good gene genetic resource foundation is laid for the cultivation of a new variety of tomatoes with high sugar content.