Wine brewing yeast strain and method for producing ethanol by efficient sugarcane juice fermentation

A technology of Saccharomyces cerevisiae and sugarcane juice, which is applied in the field of Saccharomyces cerevisiae biological fermentation, can solve the problems of slow fermentation speed of ethanol strains, high residual sugar, cumbersome production process, etc., and achieve easy process control, improved alcohol yield and simplified process Effect

Inactive Publication Date: 2009-05-20
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a kind of excellent Saccharomyces cerevisiae strain suitable for sugarcane juice fermentation, and to provide the strain to produce ethanol in sugarcane juice in view of the deficiencies such as slow fermentation speed, high residual sugar and cumbersome production process of sugarcane juice direct fermentation to produce ethanol The single-concentration continuous fed-batch fermentation process method in order to fill the gap in this production technology field in our country

Method used

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  • Wine brewing yeast strain and method for producing ethanol by efficient sugarcane juice fermentation
  • Wine brewing yeast strain and method for producing ethanol by efficient sugarcane juice fermentation
  • Wine brewing yeast strain and method for producing ethanol by efficient sugarcane juice fermentation

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Embodiment 1

[0035] Embodiment one: 20L triple fermentation tank sugarcane juice fermentation ethanol small test

[0036] 1. Small amount of yeast culture in liquid medium

[0037] Put the CCTCC NO: M208110 single colony preserved on the YPD plate into the liquid YPD medium, culture at 28°C, and culture overnight.

[0038] 2. Liquid medium to expand yeast strain culture

[0039] Take the YPD culture solution in step 1 as the seed solution, insert the processed sugarcane juice (adding concentrated sulfuric acid to adjust the pH to 3.5~4.0, adding mass ratio is 0.1% urea, mass ratio is 0.02% by 1% inoculum size) Phosphoric acid) to prepare 500ml of sugarcane juice culture fluid.

[0040] 3. Sugarcane juice fermentation in 20L triple fermenter

[0041] With the sugarcane juice nutrient solution in the step 2 as seed liquid, insert in No. 1 fermenter (volume is 20L) containing 10L to process sugarcane juice, carry out fermentation, in No. 1 fermenter, control ventilation is 100L / h, The fer...

Embodiment 2

[0042] Embodiment two: sugarcane juice fermentation daily production 10 tons of ethanol pilot test

[0043] 1. The cultivation of a small amount of yeast strains in liquid medium and the cultivation of expanded yeast strains in liquid medium are the same as in Example 1.

[0044] 2. Using sugarcane juice as raw material to ferment, the daily production of 10 tons of ethanol is pilot-tested.

[0045] Refer to attached figure 2 , after CCTCC NO: M208110 bacterial strain is expanded and cultivated to 50L, the fresh seed liquid of gain is inserted in the fermentor (volume is 120 cubic meters) containing 10 tons of sugarcane juice that has been treated to carry out fermentation. The sugarcane juice treatment method is as follows: adding concentrated sulfuric acid to the sugarcane juice to adjust the pH to 3.0-4.0, adding 0.1% urea in mass ratio, and 0.02% phosphoric acid in mass ratio. Control the ventilation in No. 1 tank, and the fermentation temperature is 26°C. When the numb...

Embodiment 3

[0046] Embodiment three: sugarcane juice fermentation daily production 10 tons of ethanol pilot test

[0047] 1. The cultivation of a small amount of yeast strains in liquid medium and the cultivation of expanded yeast strains in liquid medium are the same as in Example 1.

[0048] 2. Using sugarcane juice as raw material to ferment, the daily production of 10 tons of ethanol is pilot-tested.

[0049] Refer to attached figure 2 , after CCTCC NO: M208110 bacterial strain is expanded and cultivated to 50L, the fresh seed liquid of gain is inserted in the fermentor (volume is 120 cubic meters) containing 10 tons of sugarcane juice that has been treated to carry out fermentation. The sugarcane juice treatment method is as follows: adding concentrated sulfuric acid to the sugarcane juice to adjust the pH to 3.0-4.0, adding 0.1% urea in mass ratio, and 0.02% phosphoric acid in mass ratio. Control the ventilation in No. 1 tank, and the fermentation temperature is 29°C. When the nu...

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Abstract

The invention relates to an excellent Saccharomyces cerevisiae gxas02 and a method for carrying out scale production of ethanol by utilizing the Saccharomyces cerevisiae gxas02 as a production strain and adopting sugar cane juice as a raw material. The strain is vacuum freeze-dried and collected in China Center for Type Culture Collection with the collection No. of CCTCC NO: M208110, the collection date is July 19, 2008, the strain is the Saccharomyces cerevisiae by identification, the strain is applicable to fermenting the ethanol by using the sugar cane juice, with fast speed, low residual sugar content, high temperature resistance and alcohol resistance. A production technology adopts a single concentration continuous flow processing technology, the sugar cane juice which is pressed but not concentrated or diluted is directly fermented under the conditions without using yeast reuse and fixed yeast technology, the continuous operation is carried out on a production line with daily production capacity of 10 tons of the ethanol, the effect is good, the fermentation time is 12-16h, alcohol degree reaches 8.6-9.4(V / V) and the residual sugar is 0.2-0.3g / 100ml. The invention is characterized by convenient implementation, high equipment utilization rate, high ethanol concentration of fermented mash, high sugar utilization rate which is close to the theoretical value, and the like, thereby reducing the costs for producing the ethanol.

Description

technical field [0001] The invention belongs to the field of Saccharomyces cerevisiae biological fermentation, specifically the field of ethanol fermentation industry, and particularly relates to an excellent Saccharomyces cerevisiae strain and a method for producing ethanol by efficiently fermenting sugarcane juice. Background technique [0002] The production of fuel ethanol from sugary raw materials has always been neglected due to the high cost of raw materials. Rising crude oil prices, falling sugar prices, and the great success of Brazil's sugarcane fuel ethanol have shown the world the good market potential of sugarcane ethanol. The southern part of my country is located in the subtropical zone, with high temperature, abundant sunshine, rainy and dry autumn. It has a long history of sugarcane cultivation and has formed a large scale. It not only has the natural conditions for the development of sugarcane fuel ethanol industry, but also has formed a good raw material i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 黄日波杨登峰关妮米慧芝陆琦肖代俊黄志民黎贞崇
Owner GUANGXI ACAD OF SCI
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