Tissue sample preservative solution and preparation method thereof

A tissue sample and preservation solution technology, applied in the field of preservation solution, can solve the problems of long storage time and complex influencing factors of tissue samples, and achieve the effect of low cost, convenient use and maintaining activity

Inactive Publication Date: 2015-03-11
上海鑫曙医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, tissue samples need to be collected in different places in many cases, and the three steps of transportation, handover, and testing take longer. The tissue samples need to be stored for a longer period of time, and the influencing factors are more complicated.

Method used

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  • Tissue sample preservative solution and preparation method thereof
  • Tissue sample preservative solution and preparation method thereof
  • Tissue sample preservative solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] refer to Figure 1-Figure 2 , a tissue sample preservation solution of the present embodiment and its preparation method, in 1L tissue sample preservation solution contains: high sugar DMEM dry powder medium 13.4g, sodium bicarbonate 2.438g, DMSO 10mg, dexamethasone 100ug, insulin 8mg , penicillin 200mg, streptomycin 200mg, amphotericin 5mg, heparin sodium injection 1g, and the balance is water for injection.

[0021] The preparation method of the tissue sample preservation solution is as follows:

[0022] (a) Pour 13.4 g of high-sugar DMEM dry powder medium (brand: GIBCO, product number 12100-046) into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0023] (b) add 2.438g sodium bicarbonate;

[0024] (c) Gently stir to dissolve, add water for injection to 1L;

[0025] (d) adjust the pH value to neutrality with 1mol / L sodium hyd...

Embodiment 2

[0029] A tissue sample preservation solution, which contains in 1L tissue sample preservation solution: 13.4g high-sugar DMEM dry powder medium, 2.438g sodium bicarbonate, DMSO 5mg, dexamethasone 40ug, insulin 2mg, penicillin 100mg, streptomycin 100mg, Amphotericin 2.5 mg, heparin sodium injection 0.5 g, and the balance is water for injection.

[0030] The preparation method of the tissue sample preservation solution is as follows:

[0031] (a) Pour 13.4 g of high-sugar DMEM dry powder medium into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0032] (b) add 2.438g sodium bicarbonate;

[0033] (c) Gently stir to dissolve, add water for injection to 1L;

[0034] (d) adjust the pH value to neutrality with 1mol / L sodium hydroxide solution or 1mol / L hydrochloric acid solution;

[0035] (e) Add 5 mg of DMSO, 40 μg of dexamethasone, 2 mg o...

Embodiment 3

[0038] A tissue sample preservation solution, which contains in 1L tissue sample preservation solution: 13.4g of high-sugar DMEM dry powder medium, 2.438g of sodium bicarbonate, 20mg of DMSO, 40ug of dexamethasone, 8mg of insulin, 200mg of penicillin, 200mg of streptomycin, Amphotericin 2.5mg, heparin sodium injection 1.5g, and the balance is water for injection.

[0039] The preparation method of the tissue sample preservation solution is as follows:

[0040] (a) Pour 13.4 g of high-sugar DMEM dry powder medium into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0041] (b) add 2.438g sodium bicarbonate;

[0042] (c) Gently stir to dissolve, add water for injection to 1L;

[0043] (d) adjust the pH value to neutrality with 1mol / L sodium hydroxide solution or 1mol / L hydrochloric acid solution;

[0044] (e) Add 20 mg of DMSO, 40 μg of ...

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Abstract

The invention discloses a tissue sample preservative solution used for preserving tissue samples, such as fat, umbilical cords, placentas and the like, after collection and before separation. The tissue sample preservative solution mainly comprises following components: high-glucose DMEM dry-powder culture medium, sodium bicarbonate, DMSO, dexamethasone, insulin, penicillin, streptomycin, amphotericin and a heparin sodium injection. The high-glucose DMEM dry-powder culture medium and the sodium bicarbonate are used for maintaining osmotic equilibrium among cells inside and outside tissue, maintaining a pH value, maintaining a moistening situation of the tissue and providing nutritional components. The DMSO is a freeze-storage protective agent and can prevent freeze-injuries on the tissue samples. The dexamethasone can inhibit immunization and protect activity of stem cells in the tissue sample; the insulin can promote absorption and utilization of the tissue sample to glucose. The penicillin, the streptomycin and the amphotericin can prevent pollution from bacteria and moulds and can eliminate pollution which has occurred. The heparin sodium injection can prevent blood solidification in the tissue samples and increase a yield of the stem cells. The tissue sample preservative solution is simple in components, is low in cost, is convenient to use, can maintain activities of the stem cells in the tissue samples, such as fat, umbilical cords, placentas and the like, and can greatly reduce a time limit from collection to preparation of the tissue samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preservation solution for preserving collected tissue samples such as fat, umbilical cord and placenta. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of stem cells with multi-lineage differentiation potential, derived from mesoderm during embryonic development, and then distributed in almost all organs and tissues of the body. MSCs have the potential to differentiate into a variety of mesoderm cell lineages, including osteoblasts, adipocytes, chondrocytes, stromal cells, fibroblasts, tenocytes, etc. In the normal injury repair process of the body, MSCs can be recruited to the injury site under the induction of chemokines, proliferate and differentiate locally, and participate in injury repair and tissue regeneration through paracrine effects. MSCs have a wide range of sources and no ethical restrictions, are easy to isolate and expand in vitro,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 张炳强
Owner 上海鑫曙医疗科技有限公司
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