50 results about "Cellular viability" patented technology

The invention discloses a single cell activity evaluation method based on cell nucleus geometric parameters and a Bayesian framework, which comprises the following steps: establishing a HeLa cell activity gradient model through adriamycin induction, carrying out cell nucleus segmentation by adopting Cell pose 3.0 and extracting 26 geometric features, screening a high-confidence sample in combination with a Bayesian deep learning framework, and evaluating the activity of a single cell according to the high-confidence sample. And constructing a multi-modal feature fusion model to integrate geometric features and deep semantic features, and finally realizing unmarked and high-precision single cell activity evaluation. The method overcomes the limitation that a traditional technology depends on biochemical markers, has the advantages of being easy and convenient to operate, high in flux and high in interpretability, and provides an innovative technical means for tumor liquid biopsy and precise medical treatment.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

Disclosed herein include methods, compositions, and kits suitable for use in preservation (e.g., cryopreservation) of samples. The composition (e.g., cryopreservation reagent) can comprise: one or more cryoprotective agent(s), one or more culture media component(s), one or more carbohydrate(s), and / or one or more amino acid(s). The composition can be capable of maintaining one or more cellular properties and / or cell viability of a plurality of cells in a sample for a period of time under a storage condition. Also provided are methods of sample preservation and sample analysis. The method can comprise contacting a sample with the cryopreservation reagent, thereby generating a shielded sample. The method can comprise exposing the shielded sample to a freezing storage condition, thereby generating a cryopreserved sample.

A method of inhibiting a cancer cell growth includes contacting the cancer cell with a Cu2(OH)3NO3 / CaSiO3@g-C3N4 nanocomposite material containing graphitic carbon nitride (g-C3N4), copper hydroxide nitrate (Cu2(OH)3NO3) and calcium silicate (CaSiO3), achieving an inhibition efficiency on human breast carcinoma (MCF-7) and human hepatocellular carcinoma (HepG-2) cell growth of greater than 95% in an in-vitro cellular viability assay.

The process provided in this invention transfects primary cells with gene editing reagents using a high-volume gas-permeable cell culture device and a flow-through electroporation device under conditions that improve gene editing performance, cell yield, and drug (DP) quality characteristics for cell therapy applications. As demonstrated in the examples, primary cells edited according to the process provided herein achieved improved double-strand break (DSB) formation rates, increased frequency of homology-directed repair (HR) and non-homologous end joining (NHEJ) combinations, increased frequency of bi-allelic and mono-allelic HR events, improved cell viability, proliferative capacity, and cell fitness after gene editing, and reduced manufacturing time.

The invention relates to the technical field of biomedical processing, and particularly provides a cryopreservation method of cord blood mononuclear cells, which comprises six steps of whole blood separation, plasma inactivation, CBMC cell separation, cryopreservation liquid preparation, cell cryopreservation and detection after cryopreservation recovery. The CBMC cell separation comprises three sub-steps; performing cell cryopreservation, namely averagely dividing the separated CBMC into a plurality of parts, filling the CBMC into a cryopreservation tube according to a certain density, putting the cryopreservation tube into a programmed cooling instrument, and performing cryopreservation according to a plurality of programs; and detecting after cryopreservation and resuscitation, wherein the detection comprises three sub-steps of cell viability detection, cell recovery rate detection and cell function detection. The problems that in the cryopreservation process of a traditional method, cell viability is reduced, the number of cells is greatly reduced, cell adhesion and caking are caused, cell functions are abnormal, cell phenotypes are changed after recovery, functional activity is damaged and the like are solved, the utilization rate of the CBMC is increased, and the technical scheme is obviously superior to the traditional cell cryopreservation method; the viability, the recovery rate and the function of the recovered cells are obviously improved.

The invention provides a fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of biological analysis and detection. The fluorescent probe disclosed by the invention has a structure as shown in a formula I; the invention also provides a preparation method of the fluorescent probe and application of the fluorescent probe in cell activity detection or preparation of a cell activity detection kit. The fluorescent probe provided by the invention can reflect the cell state by migrating organelles, realizes dual-channel imaging, and can meet the actual requirements of cell activity detection.

1. The name of the design product: multifunctional enzyme label instrument. 2. The use of the design product: for nucleic acid, protein concentration, enzyme activity analysis and other conventional molecular detection; signal transduction research, cell signal event active oxygen, modification detection; cell viability, apoptosis, killing and other overall level analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

Method for determining cell viability in a cell culture of biological cells for the biotechnological production of proteins, in particular antibodies, characterized in that a fluorescent dye selected from 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonate (Bis-ANS) or 8-anilinonaphthalene-1-sulfonate (ANS) is used as a detection agent, and wherein the fluorescent dye is present in the cell culture at a concentration of 0.1 µM to 10 µM, the fluorescent dye selectively penetrates cells with damaged cell membranes and binds to intracellular proteins, the labeled cells are detected by excitation in the UV range and detection of the fluorescence in the green wavelength range, and the determination of cell viability is carried out using an automated imaging fluorescence microscope.

A method of treating cancer cells includes contacting the cancer cells with a CoO / CaSiO3@g-C3N4 nanocomposite material. The CoO / CaSiO3@g-C3N4 nanocomposite material includes hexagonal metal oxide nanoparticles comprising a CoO phase and a CaSiO3 phase dispersed on a matrix of g-C3N4 nanosheets. The hexagonal metal oxide nanoparticles have an average particle diameter in a range from 340 to 440 nm. The CoO / CaSiO3@g-C3N4 nanocomposite material has a percent inhibition for Human Breast Carcinoma (MCF-7) cells at least 55% inhibition in an in-vitro cellular viability assay.

The invention relates to an application of kunbutton alcohol as an active ingredient in preparation of a medicine or a medical beauty product for inhibiting melanin synthesis. Cell activity detection and melanin content determination show that the content of melanin in cells can be remarkably reduced under the condition of not influencing the cell activity. Tyrosinase activity detection shows that tolvaptan does not affect the catalytic activity of TYR. Protein immunoblotting experiments show that tolvaptan can down-regulate the expression levels of TYR family proteins, ommatidium-associated transcription factors (MITF), cyclic adenylate reaction element binding proteins (CREB) and melanocortin-1 receptors (MC1R).

The present disclosure provides, among other things, a method of cryopreserving and thawing cells that results in the thawed cells having high cellular viability and functionality post-thawing. In some embodiments, a large-scale method of cryopreserving cells is provided, the method comprising: (a) contacting the cells with a cryopreservation medium; (b) cooling the cells to −80° C. at a controlled rate to minimize latent heat of fusion; and (c) storing the cells in liquid nitrogen vapor phase, thereby cryopreserving the immune cells.

The present invention provides methods for collecting desired cells from a sample using countercurrent centrifugal elutriation. The method of the present invention provides a continuous elutriation workflow with optimized conditions, which improves cell purity and retention during the elutriation process. In particular, the methods of the invention use a continuous counter-current centrifugal elutriation process which does not require harvesting cells between elutriation cycles or removal of cells from the separation chamber. The method of the present invention increases the purity, cell number yield, cell recovery, and cell viability of a desired cell population to improve downstream processing. The method of the invention also reduces the volume of buffers and reagents required for the treatment and accelerates the elutriation process, thereby achieving a cost-effective and more efficient cell manufacturing workflow.

The invention relates to the field of application of radix aconiti carmichaeli polysaccharide, and provides novel application of radix aconiti carmichaeli polysaccharide, namely application of radix aconiti carmichaeli polysaccharide in preparation of medicine for treating rheumatoid arthritis. The method comprises the following steps: firstly, culturing MH7A cells in vitro, inducing the MH7A cells through monkshood polysaccharides with different concentrations, and systematically evaluating the regulation effect of the monkshood polysaccharides on the proliferation kinetics and apoptosis rate of the MH7A cells by adopting a cell activity detection kit and combining a flow cytometry analysis technology; it is proved that monkshood polysaccharides with different concentrations can inhibit MH7A cell proliferation and trigger a programmed cell death mechanism, and the monkshood polysaccharides are concentration-dependent. By dynamically monitoring the content of ROS in cells, the monkshood polysaccharide is found to be capable of reducing accumulation of ROS in MH7A cells. In addition, the phosphorylation modification level of NF-kB / P65 protein in MH7A cells and the expression level of TNF-alpha are measured through methods such as a Western Blot method and an ELISA method, and it is also proved that the monkshood polysaccharide can down-regulate the expression of the NF-kB / P65 protein and the TNF-alpha in the MH7A cells. In general, experimental results show that the monkshood polysaccharide has a strong anti-inflammatory effect on MH7A cells.

A method includes placing a sample of cells, tissue, or an organ into an oxygen imaging system, circulating a humidified gas mixture around the tissue or organ, circulating conditioned air through the oxygen imaging system to maintain a temperature around the sample, and acquiring a three-dimensional oxygen map of the sample. The oxygen map provides a quantitative measure of cell viability and functionality.

The invention discloses a method and a system for detecting cell viability and purity in chimeric antigen receptor T cell therapy. The cell viability and purity detection method in the chimeric antigen receptor T cell therapy comprises the following steps: obtaining a to-be-detected CAR-T cell sample; dyeing a CAR-T cell sample to be detected so as to obtain a dyed cell suspension; detecting the dyed cell suspension by using a flow cytometer so as to obtain a fluorescence signal; and carrying out cell activity detection and cell purity analysis according to the fluorescence signal so as to obtain cell activity information and cell purity information. The cell activity and purity detection method in the chimeric antigen receptor T cell therapy comprehensively considers multiple aspects of a cell metabolic network, including metabolic flux, metabolic pathways, metabolic network structures and the like, and can more comprehensively reflect the cell activity and purity.

Disclosed herein include methods, compositions, and kits suitable for use in preservation (e.g., cryopreservation) of samples. The composition (e.g., cryopreservation reagent) can comprise: one or more cryoprotective agent(s), one or more culture media component(s), one or more carbohydrate(s), and / or one or more amino acid(s). The composition can be capable of maintaining one or more cellular properties and / or cell viability of a plurality of cells in a sample for a period of time under a storage condition. Also provided are methods of sample preservation and sample analysis. The method can comprise contacting a sample with the cryopreservation reagent, thereby generating a shielded sample. The method can comprise exposing the shielded sample to a freezing storage condition, thereby generating a cryopreserved sample.

The present invention relates to a frozen cell formulation that can significantly improve the cell viability of cryopreserved cells, preferably stem cells. According to the present invention, the cell viability can be improved when thawing cells after freezing, thereby preventing a decrease in therapeutic efficiency that may occur during the storage and transportation of cell therapy agents.

A system and method for analyzing a sputum sample from a subject suspected of having lung cancer comprising obtaining a plurality of cells from the sputum sample from the subject, marking the plurality of cells with i) a plurality of cell lineage specific marker compositions, ii) a cell viability composition and iii) a tetra (4-carboxyphenyl) porphyrin (TCPP) composition; analyzing with the flow cytometer the plurality of cells marked with i-iii to obtain a subpopulation selected for cell size from the plurality of cells based upon an automatically selected bead size exclusion gate; from the cell size selected subpopulation, selecting a viable singlet population of cells using an automated non-debris gate and an automated singlets gate; from the viable singlet population of cells, obtaining flow cytometer values based upon the plurality of cell lineage specific marker compositions, the viability marker and the TCPP marker; applying a trained classifier to meta data from the subject and the flow cytometric values obtained; and generating, based upon the application of the trained classifier, a classification for the sputum sample wherein the classification is selected from a plurality of classification options comprising cancer and non-cancer.

The application provides an organoid viability evaluation system based on a CBAM-YOLOv3 integrated algorithm and an application, the system comprises an input display module and a detection module, the CBAM-YOLOv3 integrated algorithm is used to obtain the average diameter, the number and the product of the number and the average diameter of the organoid, meanwhile, the number of the organoids with good viability and the number of the aging organoids are counted based on the machine learning result, and the respective proportions are calculated.The application also provides built-in electronic equipment in a microscope observation device in an organoid culture process and a non-transient computer readable storage medium.The results show that the No.xDia.parameter shows good evaluation accuracy, which is highly correlated with the SA-beta-Gal positive rate and the number of passages, can accurately reflect the cell viability of the organoid, meanwhile, based on the CBAM-YOLOv3 integrated algorithm in the detection of the viability of the gastric organoid, the proportion of the organoids with good activity and the aging organoids can be output, which provides valuable reference for downstream experiments.

The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

The invention discloses a cell tracking probe as well as a preparation method and application thereof. The retention time of the existing dye is relatively short (generally only several hours); the dye is not tolerant to fixation treatment, so that the dye leaks from cells, and the problem of efflux also exists; more seriously, the novel cell tracking and cell viability fluorescent dye has fluorescence, and aims to provide the novel cell tracking and cell viability fluorescent dye which is longer in retention time, does not need an additional cleaning step and is smaller in toxicity aiming at the technical problems existing in the existing main calcein orange red dye.

The invention relates to the technical field of biology, in particular to a preparation method of mesenchymal-like stromal cells derived on the basis of cat embryonic cell spheres. According to the preparation method of the mesenchymal-like stromal cells derived on the basis of the cat embryonic cell spheres, disclosed by the invention, the cell spherical aggregate is firstly cultured, and then adherent culture and induction are performed, so that the prepared cat mesenchymal-like stromal cells are higher in quality. In addition, by optimizing the formula of the induction culture solution, the concentration of the bFGF is improved, and the cell activity and the cell quality are effectively improved.

This invention relates to the field of human cell data analysis technology, and discloses a human cell viability data analysis system based on big data, comprising: a data acquisition module for acquiring raw experimental data and transcriptome data of human cell samples to be evaluated; a preprocessing and standardization module for preprocessing the raw experimental data and outputting a three-dimensional viability tensor; a multimodal fusion module for coupling and decomposing the three-dimensional viability tensor and transcriptome data to extract a shared sample coefficient matrix; an influence feature generation module for calculating dose-influence features, time-influence features, and viability-influence features based on the shared sample coefficient matrix and the three-dimensional viability tensor; and a prediction and interpretation module for establishing a linear regression model based on the viability-influence features and preset efficacy labels, and outputting the corresponding efficacy score for the sample to be evaluated. This invention achieves standardized integration and efficient analysis of multi-source heterogeneous human cell viability data.

Disclosed herein is a 2D or 3D bioreactor, comprising a vessel forming an interior volume, wherein at least one interior surface of the vessel comprises an epoxy coating in contact with the contents of the bioreactor, wherein said epoxy coating is biocompatible by way of suitable adhesion, physical stability, absence of leaching into the contents of the bioreactor, and allows for the maintenance of cell viability. Also described are methods of manufacturing such bioreactors.

This invention belongs to the field of biomedical technology, specifically relating to a method for preparing a renal-clearable optical diagnostic probe-cell conjugate and its application. The invention involves fusing a renal-clearable optical diagnostic probe with a maleimide-modified polyethylene glycol chain, followed by incubation with Traut's-pretreated cells to obtain the renal-clearable optical diagnostic probe-cell conjugate. This method is simple, time-saving, and efficient, applicable to various renal-clearable optical diagnostic probes and cell types. Furthermore, this method has minimal impact on cell viability, disease targeting ability, and ability to cross physiological barriers. In addition, the renal-clearable optical diagnostic probe-cell conjugate constructed using this method can release renal-clearable fragments in the disease microenvironment, thereby enabling early diagnosis of various diseases through urine analysis, exhibiting high sensitivity and specificity.

A small-size electron paramagnetic resonance imaging (EPRI) instrument includes an environment-controlled resonator configured to excite and detect electron spins at a resonance frequency. The instrument enables oxygen imaging of cell-seeded wells of standard 96-wells of multi-well plate manipulated by a mechanical stage. One example is configured for long multiple-well strips. In combination with an oxygen-reporting molecular, one example provides partial oxygen pressure (pO2) maps of cells in wells that can be used for cell viability measurements and analysis of drug efficacy when used with drugs and cells.

The invention provides a method for detecting the biological activity of GDF-11, which comprises the following steps: detecting the number of MPC-11 cells through a cell activity kit, analyzing the relationship between the dosage concentration of GDF-11 and the number of the MPC-11 cells, and calculating the half inhibitory concentration IC50, thereby evaluating the biological activity of GDF-11. The method has the advantages of large detection window, simple detection method, easily available raw materials, low cost and good result stability.

The present disclosure provides, among other things, a method of cryopreserving and thawing cells that results in the thawed cells having high cellular viability and functionality post-thawing. In some embodiments, a large-scale method of cryopreserving cells is provided, the method comprising: (a) contacting the cells with a cryopreservation medium; (b) cooling the cells to −80° C. at a controlled rate to minimize latent heat of fusion; and (c) storing the cells in liquid nitrogen vapor phase, thereby cryopreserving the immune cells.