Separation method of human peripheral blood mononuclear cells

A separation method, the technology of human peripheral blood, applied in the field of cell separation, can solve the problems of high operation, influence on cell quantity and purity, etc., and achieve the effect of good differentiation ability, high expansion multiple in vitro, high cell viability and purity
CN104480070AInactive Publication Date: 2015-04-01GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Publication Date
2015-04-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a separation method of human peripheral blood mononuclear cells. The separation method combines the advantages of a hydroxyethyl starch precipitation method and a Ficoll density gradient centrifugation method. The PBMC with a purity of greater than 95% and a vitality of greater than 85% is obtained by removing most of red blood cells by the hydroxyethyl starch precipitation method, then naturally settling in air, and then further purifying the mononuclear cells by the Ficoll density gradient centrifugation method, aiming at the separation characteristic of a high-capacity blood sample. Compared with a method without natural setting in air, the vitality and the purity of the cells obtained by the separation method are higher.
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Description

technical field

[0001] The invention relates to a method for separating cells, in particular to a method for separating human peripheral blood mononuclear cells. Background technique

[0002] Cell therapy is a new technology for disease treatment that has emerged in recent years. It refers to the use of the characteristics of certain cells with specific functions, which are obtained by bioengineering methods and / or processed by in vitro expansion and special culture to enhance the ability of these cells. Immunity, killing pathogens and tumor cells, promoting tissue and organ regeneration and body recovery and other therapeutic effects, so as to achieve the purpose of treating diseases.

[0003] With its good curative effect, less side effects, more individualized and individualized unique advantages, cell therapy provides a choice, sometimes even the last choice, for the treatment of some refractory diseases. Cell therapy will play an important role in clinical treatment bo...

Claims

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