10 results about "Ficoll" patented technology

The invention relates to the field of blood detection reagents, in particular to a blood free DNA stabilizer and application thereof. Every 100 ml of the blood free DNA stabilizer comprises 0.5-5 g of a buffer agent, 2-20 g of an aldehyde fixing agent, 15-20 g of an anticoagulant, 10-50 g of a cell stabilizer, 2.5-12.5 g of a nuclease inhibitor, and the balance of ultrapure water. The pH value of the blood free DNA stabilizer is 6.8 to 7.8; the cell stabilizer is selected from one or two or more than two of polysucrose-50, polysucrose-70, polysucrose-400, polyethylene glycol-4000, polyethylene glycol-6000, polyethylene glycol-8000 and glycine betaine. Preferably, the cell stabilizer is selected from polysucrose-70, and every 100 ml of the blood free DNA stabilizer contains 10 g of polysucrose-70. The stabilizer provided by the invention is low in toxicity and low in cost, and can maintain free DNA stability and inhibit blood cell genome release at normal temperature for 10 days, so that the storage cost and the transportation cost of a blood sample are effectively reduced, and the detection accuracy of the blood free DNA is improved.

Provided is an aqueous solution for separating blood cells, the aqueous solution being capable of suppressing a change in properties even when sterilized by radiation.　This aqueous solution for separating blood cells comprises: polysucrose (A); diatrizoic acid and / or a metal salt thereof (B); and carboxylic acid different from diatrizoic acid and / or a metal salt thereof (C).

The application discloses a double-frequency ultrasonic-assisted extraction method of fig polysaccharide and application, and belongs to the technical field of natural product extraction. The method comprises the following steps: S1, preparing an ethanol solution of fig powder; S2, placing the solution in a double-frequency ultrasonic device for ultrasonic-assisted extraction, and the ultrasonic conditions comprise the following: an ultrasonic frequency of 40 kHz and 25 kHz, an ultrasonic power of 345-525 W, and an ultrasonic time of 4-12 min; and S3, obtaining fig polysaccharide extract after filtration. The method is an efficient, rapid and simple fig polysaccharide extraction method, and is particularly suitable for an industrialized production scene with strict requirements on extraction time, and the extracted fig polysaccharide has good in-vitro antioxidant activity, can be used for preparing antioxidant-related functional food, medicine and cosmetics, and has a good development prospect in the field of health products.

The invention discloses a method for separating and extracting PBMC (peripheral blood mononuclear cells), which comprises the following steps: on the premise of retaining PBMC in a sample, carrying out volume reduction treatment on a sample solution, namely, carrying out volume reduction on the sample solution to discharge a certain amount of plasma and red blood cells, and then carrying out ficoll solution centrifugation, so as to carry out one-time PBMC separation operation on a large-volume sample; the process flow time of PBMC separation and extraction is greatly shortened, the cell yield is high, the activity is high, and an effective cell acquisition way is provided for clinical and basic cytology research so as to facilitate subsequent immune cell experiments; according to the separation and extraction method, less separation liquid, washing liquid (replacement liquid) and disposable consumables are used, so that the time, consumables and reagent cost are saved.

The invention relates to a Taq enzyme antibody blocking stabilizer, which is prepared from 0.01 to 1 percent of N, N-dihydroxyglycine, 0.01 to 0.5 percent of polysucrose 70, 0.01 to 1 percent of glucan and 0.01 to 2 percent of polyvinylpyrrolidone, and the pH (Potential of Hydrogen) of the Taq enzyme antibody blocking stabilizer is 7 to 9. The Taq enzyme antibody blocking stabilizer provided by the invention solves the problems of non-specificity and performance reduction in a thermal acceleration process and / or a long-term storage process of a fully premixed reaction system of high-sensitivity qPCR containing primers, so that the debugging difficulty of the fully premixed reaction system is greatly reduced. The Taq enzyme antibody blocking stabilizer disclosed by the invention is suitable for different Taq enzymes.

The invention discloses a method for separating mononuclear cells from alpaca peripheral blood, a preparation and a preparation method, and relates to the technical field of biology, the preparation comprises a reagent A and a reagent B, the reagent A comprises 1.0 mg / mL to 4.0 mg / mL of hydroxypropyl methylcellulose, 2.0 mg / mL to 6.0 mg / mL of chitosan quaternary ammonium salt, 48.0 mg / mL to 54.0 mg / mL of polysucrose 400 and 80.0 mg / mL to 10.0 mg / mL of meglumine diatrizoate; the reagent B comprises a Percoll separating medium, a diluent of the Percoll separating medium is normal saline or PBS, and the density of the Percoll separating medium is 1.079 g / mL-1. 081 g / mL. The problems that in the prior art, the density of neutrophils and the density of mononuclear cells in alpaca peripheral blood are close, the mononuclear cells are separated and extracted by using a single or discontinuous gradient separating medium, and the separation efficiency of the neutrophils and the mononuclear cells is low can be solved. And the effect is poor.

The utility model relates to the technical field of cell separation, and discloses a centrifugal tube for quickly separating peripheral blood bone marrow mononuclear cells, which comprises a centrifugal tube, an upper internal thread is arranged on the inner wall of the centrifugal tube, and a lower internal thread is arranged on the inner wall of the centrifugal tube. Blood and ficoll liquid are isolated through the filter membrane sheet and are prevented from being mixed too much to affect the separation effect, pure mononuclear cells can be simply, conveniently and rapidly collected in the centrifugal process due to the isolation effect of the inert separation gel and the blocking function of the filter membrane sheet, and after centrifugation is completed, the mononuclear cells are collected in a direct and rapid pouring mode, so that the separation effect is greatly improved. The inert separation gel is used for isolating centrifuged red blood cells, preventing the red blood cells from being mixed into separated mononuclear cells when supernatant is poured out, improving the separation method of the mononuclear cells, improving the separation efficiency of the mononuclear cells, reducing the experiment difficulty, saving the experiment time and guaranteeing the reliability of experiment results.

The invention relates to a use method and application of a mitochondrial membrane potential probe. The use method comprises the following steps: (a) separating human peripheral blood mononuclear cells by adopting a Ficoll method; (b) carrying out flow antibody staining; (c) centrifugally collecting the cells, cleaning with a PBS buffer solution, and adding a cell culture medium to resuspend the cells; (d) adding a fluorescent probe JC-1 into the culture medium, incubating in a 5% CO2 cell incubator at 37 DEG C for 15-30 minutes, centrifuging, precipitating cells, and discarding the supernatant; adding resuspended cells, centrifuging, precipitating the cells, and discarding the supernatant; and (e) resuspending the cells in a precooled PBS buffer solution, and measuring and analyzing the membrane potential in a flow cytometer. The progress of the liver disease can be analyzed according to the significant difference, the accuracy and specificity of diagnosis are significantly improved, and a reliable basis is provided for clinical diagnosis.

This invention provides an aqueous solution for blood cell separation that can suppress changes in properties even after sterilization by radiation. The aqueous solution for blood cell separation according to the present invention comprises polysucrose (A), diatrizoic acid and / or its metal salt (B), and a carboxylic acid and / or its metal salt different from diatrizoic acid (C).

The invention relates to the technical field of molecular diagnosis, in particular to a multiple reverse transcription polymerase chain reaction detection reagent buffer solution and a kit. According to the invention, multi-factor comprehensive research is carried out on multiple reverse transcription polymerase chain reactions, especially on the performance of a one-step RT-PCR reaction system constructed by taking Z05 enzyme as a bifunctional enzyme raw material, so that the influence of a polyhydroxy compound as an additive on various performance indexes of the reaction system is fully analyzed; a specific polyhydroxy compound (Ficoll 400) is creatively selected to be compounded with pinotriose to serve as a reaction buffer reagent additive, a synergistic interaction effect is generated, and especially under a buffer reagent system preferably constructed by a Tricine buffer solution, the final fluorescence value of an RT-PCR fluorescent quantitative reaction system is more efficiently increased, the signal-to-noise ratio is increased, and the detection accuracy is improved.