24 results about "Cell stress" patented technology

Provided are a small protein UFD1s and use thereof in the prevention, treatment, and diagnosis of non-alcoholic steatohepatitis. The provided small protein UFD1s can regulate energy metabolism and combat cell stress, and UFD1s can be used as an auxiliary diagnostic marker for non-alcoholic steatohepatitis (NASH). Meanwhile, the deletion or low expression level of UFD1s is related to the development and progression of NASH. By expressing UFD1s in vivo by means of a constructed expression vector and a method, its effect in preventing and / or treating NASH can be exerted, thereby providing a new idea for the prevention and / or treatment of NASH.

Tissue-specific progenitor cells representing the endoderm, ectoderm, and mesoderm lineages are generated from pluripotent sources. Progenitor cell generation is accomplished by contacting pluripotent stem cells with tissue-specific exosomes, microRNAs, proteins, and peptides, obtained by stressing the tissue under in vitro conditions. In vitro-generated tissue organoids are used as a source of "differentiation factors" for generating individualized progenitor cells. For example, to generate lung progenitor cells, allogeneic stem cells are implanted into decellularized cadaveric lung tissue to form lung structures, which are then exposed to various cellular stressors and conditioned media to differentiate the pluripotent stem cells into progenitor cells. Extracellular matrix can be obtained from decellularized structures seeded with regenerating cells and used to generate organoids, which are then subjected to stress to produce differentiation factors.

The invention discloses an oscillation device for three-dimensional multiplication culture preparation of stem cells, and relates to the technical field of cell culture equipment.The oscillation device comprises a cylindrical culture chamber with a space for oscillation in the middle; the spiral main shaft assembly is arranged in the culture chamber; through cooperative operation of the main shaft driving mechanism, the bidirectional driving assembly and the twisting assembly, the spiral main shaft assembly is driven to generate composite three-dimensional motion of rotation, axial compression and radial twisting at the same time, and the problems that in the prior art, a mechanical stimulation mode is single, real-time sensing cannot be achieved, and cell stress is uneven are solved. The limitation that a traditional device can only provide a single oscillation mode is broken through, complex mechanical environments such as in-vivo vascular pulsation, skeleton bearing and muscle contraction can be highly simulated, and an intelligent culture platform capable of simulating the complex in-vivo mechanical microenvironment and achieving real-time sensing and accurate regulation and control is formed; and the quality and efficiency of stem cell amplification and the functional maturity of tissue engineering products are remarkably improved.

Disclosed herein are polynucleotides that can sustain payload expression and / or induce payload expression during cellular stress. Also provided herein are compositions comprising the disclosed polynucleotides, and methods of making and using the same.

Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g / L or at a greater concentration than 1.8 g / L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

The invention discloses a tannic acid molybdenum coordination coated DNase nanocapsule as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the preparation method, molybdenum and tannic acid are utilized to form an MPN nanocapsule, DNase II is carried, and the Mo-TA-coated DNase II nanocapsule is constructed; the positively charged metal phenolic network nanoparticle (MPN NPs) shell has remarkable advantages in the aspect of adsorbing circulating free DNA (cfDNA), the porous structure and rich surface functional groups provide high adsorption capacity and rapid adsorption kinetics, and the cfDNA can be efficiently captured. MPNs also has the characteristics of good biocompatibility, easy carrying and the like, and the MPNs has good PH responsiveness, so that DNase II can be accurately released in lysosome and effectively hydrolyzed in cells, the inflammatory reaction caused by cfDNA is blocked by using cfDNA, and meanwhile, the interburial effect of macrophages is recovered; mo and TA have oxidation-reduction ability, can effectively improve the stress state of cells and mitochondrial dysfunction, obviously inhibit the generation of cfDNA, and realize the full-period management from symptom treatment to root cause treatment.

The application discloses an application of a small molecule inhibitor of stress granules in preparation of a tumor treatment drug, and relates to the technical field of biological medicines.The small molecule inhibitor of stress granules, i.e., compound UA-0007373, is obtained by high-throughput screening of "ChemDiv Protein-Protein Interaction (PPI) Library". The small molecule inhibitor can effectively inhibit the formation of cell stress granules caused by G3BP1, thereby reducing the drug resistance of tumor cells. The small molecule inhibitor can effectively inhibit the formation of stress granules induced by an anticancer drug through cell experiments. Therefore, the application provides a new auxiliary compound for the chemical drug treatment of cancer, and can provide a new technical thought for the research and development of cancer drugs.

The invention provides a cell biomechanics and migration path detection method based on a 1S1R neuromorphic sensitive array, which comprises three parts of living cells, an extracellular matrix and a neuromorphic sensitive array, and a cell matrix and a unit neuromorphic sensitive device in a unit area form a 1S1R unit. Local current is changed through the mechanical action of the cells and the extracellular matrix, and the sensing of mechanical stress between the cells and the extracellular matrix in a unit area is realized by utilizing the specific memory effect of the neuromorphic sensitive device. Finally, cell stress changes of a plurality of areas are achieved through the neuromorphic sensitive array, and detection of cell migration paths and cell biomechanical behaviors induced by multi-modal signals and environments are achieved. By preparing the 1S1R-based neuromorphic sensitive array, a small-size and high-efficiency cell migration and biomechanical research scheme can be realized, so that a neuromorphic sensitive device shows huge application potential in perception and research of biological microscopic phenomena such as cell migration.

The invention discloses a mitochondrial function detection method and application thereof, and a mitochondrial function detection kit. According to the mitochondrial function detection method provided by the invention, the mitochondrial function is judged by detecting the release condition of mitochondrial DNA into cytoplasm under high-salt stimulation. According to the invention, the characteristic that the mitochondrial DNA is sensitive to external stimulation (high salt) is utilized, and the leakage condition of the mitochondrial DNA is directly detected, so that the mitochondrial function is judged. The detection method does not need large-scale equipment, is low in cost and is suitable for large-scale detection; the operation is simple and convenient, only high-salt treatment and a conventional DNA level detection method (such as qPCR) are needed, and the method is economical and efficient; the sensitivity is high, and the mitochondrial DNA release amount can be detected through qPCR and the like so as to detect the early reaction of cell stress; the release of the mitochondrial DNA can be continuously detected, and the time dynamic state and the continuity of the release of the mitochondrial DNA can be observed, so that the mitochondrial function can be dynamically evaluated and monitored.

The invention belongs to the technical field of biological medicines, and particularly relates to application of neratinib in preparation of a medicine for preventing and treating noise-induced deafness. According to the specific technical scheme, the application of the neratinib in preparation of the medicine for preventing and treating hearing impairment / deafness is provided. According to the invention, the fact that neratinib can play a hearing protection role by specifically regulating and controlling cochlea immune microenvironment is found for the first time, and the hearing protection role is specifically realized by the following mechanisms: regulating and controlling biological effects such as cell signal channels, inflammatory response and cell stress response, protecting hair cell structures, and reducing cochlea basilar membrane outer hair cell loss caused by noise exposure. Based on the new discovery of the invention, the neratinib can be used for preparing medicines for preventing and treating hearing impairment and deafness caused by noise.

The present invention provides a labelled cell stress marker for use in identifying or predicting angiogenesis in a subject. Also provided is a method of identifying or predicting angiogenesis in a subject, the subject having been administered a labelled cell stress marker. The method comprises the steps of (a) generating an image from the subject using a first imaging device and (b) identifying labelled cell stress marker positive cell(s) in the image. Angiogenesis is identified or predicted if labelled cell stress marker positive cell(s) are identified in the image.

A cell stressor device for use in analyzing a biomechanical response of cells or tissues may include a body including opposing ledges. A bottom of each ledge may be attached to a bar, and the pair of ledges and the bar may form a window having an opening facing away from a top of the body. The device may include a bender shaped and dimensioned to be inserted into the window. A length and height of the bender may be less than a length and height of the window. The device may include a beam shaped and dimensioned to be inserted into the window between the bender and the ledges to contact the ledges. The device may include means for moving the bender alternately away from and back toward the bar to cause a top of the bender to impinge upon a bottom of the beam.

The invention belongs to the technical field of cell mechanics, and particularly relates to a single cell dynamic mechanical loading system based on magnetic beads and a bidirectional static magnetic field and a force regulation and control method. The invention provides a single cell dynamic mechanical loading system based on magnetic beads and a bidirectional static magnetic field and a force regulation and control method. The system is composed of a magnetic field system module, a dynamic regulation and control module and a cell stress feedback module. Through the system, single-cell-level pulling / pressing two-way mechanical stimulation is achieved for the first time, magnetic bead concentration-magnetic field direction cooperative adjustment is achieved, and limitation of a traditional multi-cell loading mechanical mode is broken through; the system is used for carrying out mechanical regulation and control on single cells, can be used for single cell mechanical response research (such as stem cell differentiation and tumor cell migration), drug screening (detecting the influence of drugs on cell mechanical characteristics) and construction of a dynamic mechanical microenvironment in tissue engineering, and has a good application prospect.

This invention discloses a method for constructing a cell nucleus model based on Abaqus-Python, relating to the field of cell stress analysis technology. This method utilizes Abaqus finite element software to draw a cell nucleus model and analyze its mechanical properties. Simultaneously, it employs Python for secondary development, simplifying the model drawing process through scripting and improving the efficiency and accuracy of model creation. This model can be used to calculate the stress distribution of the cell nucleus's main structures—the nuclear membrane and nuclear lamina—during the process of nanoneedles penetrating the cell nucleus, and to optimize nanoneedle parameters by analyzing the stress concentration of the nuclear membrane during penetration.

The present application relates to the technical field of biological fermentation, in particular to an unattended AI intelligent biological fermentation tank and a control method, comprising: a key data acquisition module for acquiring a key metabolite concentration time sequence and a microbial microscopic morphology image sequence in the tank; wherein the microbial microscopic morphology image sequence contains branch degree and vacuolization characteristic data of mycelium / cells; a flow state feature extraction module for constructing a metabolic flow intensity curve based on the key metabolite concentration time sequence.The present application synchronously acquires the key metabolite concentration time sequence and the microbial microscopic morphology image sequence by constructing an in-vitro circulating sampling flow path, and performs time stamp alignment, and this dual perception mechanism can not only capture chemical changes of metabolites, but also can early warn cell stress response through microbial morphology, thereby improving the sensitivity and foresight of state perception, and solving the problems of state monitoring lag and one-sidedness.

The invention discloses a quinolyl fluorescent probe containing indometacin as well as a preparation method and application of the quinolyl fluorescent probe. The structural formula of the probe is shown in the specification. According to the probe constructed by the invention, a quinoline fluorophore is coupled with a COX-2 targeting group indometacin through a variable-length carbon chain connector, so that the fluorescent probe has relatively large Stokes shift gt; and the wavelength is 160 nm. Wherein the probe ICP-1 shows excellent environmental sensitivity due to limited intramolecular rotation, selectivity and open-type fluorescence detection on COX-2 can be realized, and the probe successfully visualizes RAW 264.7 macrophages induced by lipopolysaccharide, PC12 neuronal cells stressed by glutamic acid and COX-2 up-regulation in an AD zebra fish model. Besides, ICP-1 is effectively used for screening the COX-2 inhibitor to confirm the neuroprotective effect of hararine, and it is indicated that ICP-1 is a powerful tool for real-time neuroinflammation imaging and has application value in neurodegenerative disease research.

The invention belongs to the related technical field of micro-fluidic cell sorting, and discloses a micro-fluidic flow cell sorting system and method based on a cell deformation image, and the micro-fluidic flow cell sorting system comprises a micro-fluidic chip, the micro-fluidic chip comprises an inertia focusing structure, a cross-shaped cell stress deformation runner structure, a cross-shaped liquid drop generation structure, a liquid drop electrostatic separation structure and a liquid drop collection structure which are connected in sequence; the inertial focusing structure is used for focusing cells, so that the cells orderly enter the cross-shaped cell stress deformation runner structure; the cross-shaped cell stress deformation flow channel structure is used for enabling cells to deform through tensile shear force of fluid in a flow channel on the cells; the cross-shaped liquid drop generation structure is used for completely wrapping cells in liquid drops to form a completely closed liquid environment; the liquid drop electrostatic sorting structure is used for charging liquid drops as required, so that cell sorting is realized through a deflection electric field; and the liquid drop collecting structure is used for collecting the separated liquid drops. According to the invention, the integration level is improved.

The invention discloses a ubiquitin ligase TRIM15 inhibitor and application thereof, and belongs to the technical field of medicinal chemistry. Researches show that TRIM15 plays a role in various cell processes, especially in ubiquitination, immune regulation and cell stress reactions, the invention screens and identifies the compound shown in the following formula, or the use of the hydrate, solvate, pharmaceutically acceptable salt, pharmaceutically acceptable ester or prodrug thereof in preparation of drugs, the medicine is used as an ubiquitin ligase TRIM15 inhibitor for inhibiting the activity of the ubiquitin ligase TRIM15; researches confirm that the ubiquitin ligase TRIM15 inhibitor shows an obvious anti-tumor effect in vivo and in vitro, is expected to be applied to the fields of anti-tumor, immunoregulation and the like, and is combined with the existing clinical medicines to improve the treatment effect.

The application provides a kind of passive human cavity pressure measurement method and system based on multi-stable cell string, including receiving the deformation data of multi-stable cell string, wherein the multi-stable cell string generates deformation according to the pressure change of the cavity to be measured;Stress analysis is carried out on the deformation of the multi-stable cell string, and cell stress data is obtained;The mapping relationship between the deformation field and the pressure field is established based on the cell stress data;According to the mapping relationship, the deformation data of the multi-stable cell string is pressure inversion, and the pressure data of the cavity to be measured is obtained.The application can respond to the pressure change in the human cavity through the deformation of the multi-stable cell string, then through the mapping relationship between the deformation field and the pressure field, the pressure data and the deformation data are calibrated or converted, the pressure indicators after pressure measurement are quickly read and converted, and the pressure measurement efficiency of the human cavity is improved.

The invention relates to a cell stress pulse detection ultrafast fluorescent probe group and application thereof, belongs to the field of organic fluorescent probes, and provides specific micromolecular fluorescent probes C1 and T1 for detecting Cu < 2 + > and tyrosinase of CSP, the probe C1 is designed based on a specific recognition group of Cu < 2 + >, and the probe T1 is designed based on an active cavity structure of tyrosinase and a specific recognition group of the tyrosinase. All the compounds have ultrafast response speed, excellent light stability, good pH stability and anti-interference ability to other ions and amino acids in cells. After the probe C1 reacts with Cu < 2 + >, a lactam spiro ring is opened to emit strong near-infrared fluorescence, similarly, after the probe T1 reacts with tyrosinase, a spirolactone ring is opened, and a near-infrared fluorescence signal is remarkably enhanced. The probe C1 and the probe T1 can specifically and rapidly detect the Cu < 2 + > content and the tyrosinase activity in vitro, and CSP detection is achieved by monitoring the changes of Cu < 2 + > and tyrosinase in cells under the stress condition in real time.

The present invention provides a means or a system for screening a drug for treating or preventing age-related hearing loss. Specifically, the present invention provides a method for screening a drug for treating or preventing age-related hearing loss, said method comprising: a step for preparing a pluripotent stem cell that has a genetic variant correlating with age-related hearing loss; a step for inducing the differentiation of the pluripotent stem cell into an inner ear cell; a step for applying cell stress to the inner ear cell in the presence of a factor to be tested; and a step for evaluating the survival of the inner ear cell.

The application belongs to the technical field of animal models, and provides a method for constructing a mouse testis tissue stress granule animal model, which comprises the following steps: after the mouse is anesthetized, the testis and epididymis of one side of the mouse are taken out; a sodium arsenite solution containing fast green dye is injected into the testis of the mouse through the entrance of the rete testis, and the concentration of the sodium arsenite solution is 30-50 mM; the wound of the mouse is sutured, the mouse is killed after the mouse recovers for 3 hours, and the testis tissue is separated for an immunofluorescence experiment; when obvious stress granules are formed, it is indicated that the model construction is successful. The application also provides an application of the method for constructing an animal model in identifying the generation of stress granules of spermatocytes and in identifying the generation of RNA binding protein SERBP1. The application provides a method for exploring the role of SERBP1 protein in the stress granules of the mouse testis tissue, a related mechanism and a possible molecular pathway, and provides a new theoretical basis for guiding the generation and recovery of cell stress granules under special conditions.

The invention discloses an inversion analysis method applied to cell stress research, and relates to the technical field of microfluidics, and the method comprises the following steps: firstly, constructing a multi-stage bidirectional fluid-solid coupling kinetic model of a fluid-droplet-particle system, and realizing iterative analysis from suspension liquid, droplet deformation and droplet content particle stress; secondly, a high-speed microscopic imaging system is used for obtaining dynamic distribution of a flow field in the micro-flow tube, and an experimental observation database is constructed; thirdly, establishing a numerical model, and comparing a simulation result of the numerical model with experimental observation data; and finally, quantizing deviation between numerical simulation and experimental data through an inversion function, optimizing model parameters, and realizing accurate inversion of the size of the cell surface stress in a micro-droplet wrapping state. The method is high in detection precision and good in inversion reliability.

The invention relates to application of a compound for inhibiting SASP in preparation of an anti-aging product, and belongs to the technical field of new application of known medicines. The compound for inhibiting the SASP is pinocembrin, and the pinocembrin is used for inhibiting replicative senescence of cells and cell stress senescence induced by etoposide, adriamycin and H2O2. The invention researches that pinocembrin realizes an anti-aging effect by inhibiting characteristic senescence-related secretion phenotype factors of senescence cells, reducing expression of senescence-related cell cycle arrest marker genes and relieving the senescence condition of the cells, and does not have an effect of eliminating and damaging the cells.