56 results about "Cell stress" patented technology

Disclosed are a phase change memory with improved retention characteristic of a phase change device, and a method for refreshing the phase change memory. The fact that a memory is a DRAM interface compatible memory is exploited. There are provided dummy cells stressed in accordance with the number of times of read and write operations. Changes in the resistance value of the dummy cells are detected by comparator circuits. If the resistance value have been changed beyond a predetermined reference value (that is, changed to a low resistance), a refresh request circuit requests an internal circuit, not shown, to effect refreshing. The memory cells and the dummy cells are transitorily refreshed and correction is made for variations in the programmed resistance value of the phase change devices to assure the margin as well as to improve retention characteristic.

The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time; quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.

The invention provides a method for estimating a secondary cell SOH value and testing the residual service life and relates to a method for predicting the residual service life of a secondary cell. The predicting method comprises the following steps of acquiring performance degradation concomitant variable data of the secondary cell in real time, establishing a secondary cell degradation concomitant variable state process model and estimating model parameters in real time, calculating a degradation quantity value of the secondary cell, calculating a result and cell ex-factory data in real time, estimating a current SOH value of the secondary cell in real time, establishing a secondary cell stress-degradation united model and estimating model parameters, and calculating the residual service life of the secondary cell under different using conditions according to preset using standards of the secondary cell in real time. By means of the method for estimating the secondary cell SOH value and testing the residual service life, the accuracy and the reliability of health status SOH estimation and residual service life prediction of the secondary cell can be improved, the using and maintenance efficiency of the cell is improved, and the service life of the cell is prolonged.

A novel class of agents has been identified to serve as cell-guard agents and/or target-specific supplements to increase cell quality and yield, as well as select for target cell populations. Laboratory experiments have demonstrated the use of cell-guard agents and/or target-specific supplements in the bioprocessing of cells as well as in selecting out a desired cell population. Several potential additive agents (both natural and synthetic) have been identified during these studies, including Vitamin D₃, NAC, resveratrol, salubrinal, AKT, and tunicamycin (among others) that hold promise for application in cell models. In one embodiment, hypothermic stress regimes are utilized. In another embodiment, normothermic conditions are utilized while other stressors are tested in the processing. The methods of maintaining mass cell cultures and/or selecting out particular cell populations for further research and clinical use represents an important step in therapeutic discovery. The cell-guard agents are process-matched and cell-matched to protect and/or select particular cells during laboratory and clinical manipulation. The components modulate cell stress and survival pathways.

The present invention relates to compositions and methods for the treatment of Alzheimer's disease and related disorders. More particularly, the invention relates to combined therapies that modulate cell stress response for treating said disease.

The present invention discloses a method of isolation, pooling and further culturing of Mesenchymal Stem cells (MSC) for clinical application. Present invention also discloses the method of establishing Master Cell bank, followed by Working Cell Bank from which the final therapeutic composition referred to as Investigational Product/Investigational Medicinal Product comprising of allogenic bone marrow-derived MSC is formulated for clinical applications. Present disclosure also discloses a robust manufacturing process for consistent production of clinical grade Mesenchymal Stromal cells (MSCs). The process enables production of highly viable potent cells. The process steps relating to preparation of media, cell seeding, harvesting are fine tuned to achieve consistency in cell yield, superior cell viability, purity, improved cell proliferation, high cell recovery, low HLA-DR expression, reduction in culture duration. The viability and purity of cells are further improved by optimized wash process without cell loss/cell stress. The disclosure further provides a method of cyrostoring MSCs at high cell density without affecting the viability of cells. It further provides economical means to store and transport at −80° C.

The invention discloses a novel cucumber SNP molecular marker. The mark number of the novel cucumber SNP molecular marker is SLAF7695. According to the novel cucumber SNP molecular marker, the simplified genome depth sequencing technology is utilized, 73100 SLAF labels are obtained totally, 5355 polymorphic markers of three types including SNP, EPSNP and INDEL are obtained through polymorphic analysis, and 140 different markers closely related to cucumber powdery mildew resistance are obtained and mainly concentrated on the first chromosome and the sixth chromosome. Two candidate areas related to powdery mildew resistance characters are successfully located, seven special markers closely linked with cucumber powdery mildew resistance are obtained, 28 genes and related annotation information are obtained through gene annotation, and five genes are related to the functions of defense response, harm response, toxin catabolism, cell stress response and the like. The novel cucumber SNP molecular marker provides a theoretical foundation for exploration of cucumber powdery mildew resisting mechanism and molecular marker assisted selective breeding.

The invention discloses a microarray chip for detecting protein expression in a cell stress reaction and its preparation method, kit and detection method. The microarray chip comprises seven stress reaction markers, can ensure inner cell protein expression change corresponding to a cell stress reaction under different stimulation conditions and realizes fast screening and evaluation. A hydroformylation-modified glass sheet is used as a solid carrier so that specific antibody bonding is stable and activity is high. The microarray chip can effectively eliminate the false positive and false negative results of the spectral chip test. The cross-reaction test result shows that the microarray chip has high index detection sensitivity and good specificity. The preparation method has simple processes, realizes fast detection, finishes the whole process in 2d, has high sensitivity and good specificity, realizes a low production cost and has a wide industrialization prospect.

The invention relates to an ocean ecologic toxicology research platform based on a microfluidic chip. The invention provides a microfluidic chemotactic chip. The microfluidic chemotactic chip is formed by the following units: a chip cell culture unit, a chip composite pollution generation unit, a chip pollutant concentration gradient generation unit, a chip control unit and a chip detection system. By integrating various pollutant composite units, the pollutant concentration gradient generation unit, and the array cell culture, cell stress, marking and corresponding detection units on the chip, the multi-index, multi-parameter and multi-response-event analysis for the damage action process of the environmental pollution for the marine organisms can be realized, the toxicity effect of the pollutants can be determined, and a great amount of important toxicology information can be acquired in a single experiment. The ocean ecologic toxicology research platform is significant in functionalcharacteristics in integration and flux compared with the traditional ecologic toxicology toxicity experimental method, and is simple in operation and low in cost; and meanwhile, the platform also has certain universality and can be extensively used for other research fields of the marine environmental sciences.

The invention discloses a protein related to cell stress, and a coding gene and application thereof. The protein is 1) a protein which has an amino acid sequence shown as a sequence 2 in a sequence table, or 2) a protein which is related to the cell stress and derived from the protein 1) with the amino acid sequence shown as the sequence 2 in the sequence table by substituting and/or losing and/or adding one or more amino acid residues. Cells which overexpress an OCT4B-190 protein are constructed; results prove that the apoptosis of the cells which overexpress the OCT4B-190 protein under the stress condition is obviously reduced, namely the OCT4B-190 is the protein related to the cell stress; and the OCT4B-190 protein and the coding gene thereof can be used for inhibiting cell apoptosis under the stress condition.

The present invention relates to compositions and methods for the treatment of Alzheimer's disease and related disorders. More specifically, the present invention relates to novel combinatorial therapies of Alzheimer's disease and related disorders. In particular, the invention concerns compounds which, alone or in combination(s), can effectively modulate synapse function and/or angiogenesis and/or cell stress response. The invention also relates to methods of producing a drug or a drug combination for treating Alzheimer's disease and to methods of treating Alzheimer's disease or a related disorder.

The invention discloses a natural scorpion venom polypeptide. The natural scorpion venom polypeptide is a novel protein separated from scorpion venom of Buthus martensii Karsch and has a novel medicinal purpose on the aspect of bone marrow haematopoietic functional protection. A preparation method of the natural scorpion venom polypeptide comprises the following steps of (1) collecting the scorpion venom of Buthus martensii Karsch; (2) separating SVPIV; (3) purifying BmKpp; (4) measuring the molecular weight and amino acid sequence of of BmKpp; and (5) measuring the bone marrow haematopoietic functional protection effect of BmKpp. BmKpp is a novel polypeptide from scorpion venom of Buthus martensii Karsch and has a remarkable bone marrow haematopoietic functional protection effect. BmKpp is single in component, precise in molecular weight, clear in total amino acid sequence, remarkable in cell proliferation promoting effect as well as standard and controllable in quality in the purification process so as to be beneficial to large-scale production. BmKpp has remarkable advantages and prospects on the aspects of bone marrow haematopoietic functional protection and development of drugs for preventing/protecting cell stress injury (radiotherapy, chemotherapy and the like).

The invention provides compositions and methods for diagnosing tumors and augmentic therapeutic intervention and measuring response to cell stress using sphingomyelin containing liposomes. The liposomes can include radiotracers, contrast agents, chromophores, dyes, enzyme substrates, therapeutic agents, chemotherapeutic agents or DNA segments. The indicators enable measurement of the extent of cellular release of Acid SMase at a localized site of cell stress. The nanoparticles have the capacity to locally release their contents, be it imaging (for diagnosis) or therapeutic agents (to augment therapy).

The embodiment of the invention discloses a micro-fluidic chip and a manufacturing method thereof. The micro-fluidic chip comprises a pressurization layer comprising a vent hole and a vent groove, wherein the vent hole is used for introducing gas into the vent groove, and the vent groove generates a gas pressure based on the introduced gas; a capture layer comprising a capture channel that is usedfor introducing a plurality of samples to be pressurized; and a deformation layer arranged between the pressurization layer and the capture layer and used for generating deformation according to theair pressure so as to apply mechanical force to the plurality of samples to be pressurized in the capture layer. According to the micro-fluidic chip provided by the embodiment of the invention, cell spheres are extruded through the capture layer, the deformation layer and the pressurization layer, and the extrusion force on cells can be controlled by controlling the pressure of gas introduced by the pressurization layer, so that the controllability of cell stress is realized.