7 results about "Cytoprotection" patented technology

The invention discloses a peony polyphenol extract as well as a preparation method and application thereof, and relates to the technical field of biological medicines. Dried flowers of paeonia ostii are used as raw materials, peony polyphenol powder is obtained through an ethanol extraction-pore resin purification method, and the polyphenol content of the peony polyphenol powder is larger than 80% through determination of a folin-phenol method. Paeonia suffruticosa polyphenol is rich in more than 30 kinds of main active ingredients, and comprises flavonoids, phenolic acids and polyphenol derivatives, and flavonoids and phenolic acid compounds account for 81.25% of the total number of identified ingredients. The peony polyphenol plays a role in resisting cervical cancer through double ways of inducing HeLa cell apoptosis and activating HeLa cell autophagy, and the problem of drug resistance of a single-target drug can be effectively solved; the compound has a good application value in anti-cancer drugs. Meanwhile, the peony polyphenol shows excellent antioxidant activity in various free radical models, remarkably inhibits ROS (reactive oxygen species) generation at a cellular level, relieves oxidative stress, has a cell protection effect, and can be used as a natural antioxidant and a cell protection agent.

The invention discloses a method for improving the stability of glycyrrhizic acid by using zein and astragalus polysaccharide and application of the glycyrrhizic acid in preparation of a liver protection medicine. The method comprises the following steps: (1) dissolving raw materials; (2) mixing the raw materials; and (3) preparing and freeze-drying the nanoparticles. According to the zein / astragalus polysaccharide / glycyrrhizic acid nano-particles prepared by the method disclosed by the invention, in an in-vitro simulated digestion experiment, the biological accessibility reaches 72.84% and is obviously improved compared with that of free glycyrrhizic acid (30.70%). The free radical scavenging rate of the nano-composite is 66.23-73.34%, and the nano-composite can still keep more than 40% of activity after being stored for two days, and shows good pH, ionic strength, temperature and storage stability. Cell experiments show that under the concentration of 100 [mu] g / mL, the nano-composite can increase the survival rate of AML-12 cells to 95.50%, which is significantly superior to that of free glycyrrhizic acid (83.77% when the concentration is 20 [mu] g / mL), and the nano-composite has stronger stability and cell protection effect.

This invention relates to a drug for treating ischemia-reperfusion injury and its application. The drug activates the CCL2-chemokine receptor 2 signaling axis, triggering the downstream G protein αi2 subunit-mediated phosphatidylinositol 3-kinase / protein kinase B signaling pathway. This, in turn, synergistically regulates the antioxidant defense system driven by nuclear factor erythroid 2-related factor 2 and the B-cell lymphoma 2-mediated anti-apoptotic pathway, thereby treating the ischemia-reperfusion injury. The results of this study establish a novel therapeutic strategy for testicular ischemia-reperfusion injury based on engineered exosomes, and simultaneously reveal the previously undiscovered cytoprotective role of the CCL2 / CCR2 signaling axis in non-immune cells.

The invention provides application of UM729 in preparation of a medicine for preventing and / or treating myocardial cell hypoxia injury, and belongs to the technical field of biological medicine. In-vitro experiments prove that in a safe concentration range of 0.01 mu M to 1 mu M, the pyrimidine indole derivative UM729 can significantly improve the survival rate of myocardial cells under an anoxic condition, and exerts definite antioxidant and cell protection effects by inhibiting the release of lactic dehydrogenase (LDH), reducing the content of malondialdehyde (MDA) and enhancing the activity of superoxide dismutase (SOD). The discovery provides a new medical application for the UM729, and provides a new candidate compound and scheme for developing medicines for preventing and treating myocardial hypoxia injury related diseases such as myocardial infarction and plateau hypoxia.

Lysosomal membrane permeabilization is an evolutionarily conserved hallmark of stress-induced cell death. Here the inventors show that the major stress-inducible heat shock protein 70 (Hsp70) enhances cell survival by stabilizing lysosomes through a pH-dependent high affinity binding to an endo-lysosomal anionic phospholipid bis(monoacylglycero)phosphate (BMP; also referred to as lysobisphosphatidic acid). The positively charged ATPase domain of Hsp70 is responsible for the binding but the substrate-binding domain is also required for effective stabilization of lysosomes. Importantly, the cytoprotective effect can be obtained by endocytic delivery of recombinant Hsp70 and specifically reverted by extra cellular administration of BMP antibodies or Hsp70 inhibitors. Thus, this protein-lipid interaction opens exciting possibilities for the development of cytoprotective and cytotoxic lysosome-specific therapies for the treatment of degenerative diseases and cancer, respectively.

The present invention relates to a composition comprising TES trioleate and oleamide serinol as active ingredients for alleviating and preventing cellular senescence. According to the present invention, the composition comprising TES trioleate and oleamide serinol exhibits cytoprotective effects on skin cells damaged by UV and inhibitory effects on generation of reactive oxygen species. According to the present invention, the composition comprising TES trioleate and oleamide serinol exhibits the effects of protecting skin cells damaged by UV, suppressing generation of senescence-associated marker proteins (SA-β-gal), and inhibiting formation of a senescence-associated secretory phenotype (SASP).

Orally administered low-dose formulations for medium chain di-carboxylic acids have unexpectedly a numerous of beneficial cytoprotective effects in a variety of cells. In particular, contemplated formulations compositions and methods reduce, reverse, and / or prevent liver inflammation, hepatic steatosis, and / or liver fibrosis in NASH, improve blood glucose control, and provide additional cytoprotective benefits such as stimulation of mitochondrial biogenesis, increased SIRT and NAMPT levels, increased NAD+, antioxidant capacity, and / or reduction of DNA damage.