84 results about "Cell membrane permeability" patented technology

The invention discloses a device and system for conducting electric field treatment on the digestive tract, the device for conducting electric field treatment on the digestive tract comprises a slender catheter, a supporting body located at the far end of the catheter, an electrode array film and a pulse generator, the electrode array film and the pulse generator are arranged on the supporting body, and the output end of the pulse generator is coupled with an electrode pair on the electrode array film. A sequence pulse wave output by the pulse generator comprises a plurality of groups of nanosecond pulse strings, and the time interval between the adjacent nanosecond pulse strings is 1us-10000 us; the nanosecond pulse string is composed of a plurality of nanosecond pulse pairs, and the time interval between every two adjacent nanosecond pulse pairs ranges from 50 ns to 10000 ns; the nanosecond pulse pair comprises positive pulses and negative pulses which appear alternately, the pulse width of the positive pulses and the pulse width of the negative pulses are 10-1000 ns, and the time interval is 10-1000 ns. The cell membrane permeability and the cell death efficiency can be remarkably improved, and the limitation of the traditional IRE technology is broken through.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

The present disclosure provides a carrier peptide fragment with excellent cell membrane permeability. The carrier peptide fragment disclosed herein includes the following amino acid sequence set forth in SEQ ID No.: 1: KKRTLRKSNRKKR, and a lysine residue at a 12th position from an N-terminus of the amino acid sequence is a D-form, and the other amino acid residues are L-forms.

An object of the present invention is to provide a method for predicting cell membrane permeability of a cyclic peptide, which enables versatile design of a cyclic peptide with cell membrane permeability. According to the present invention, there is provided a method for predicting cell membrane permeability of a cyclic peptide, the method including a first step of acquiring a structure of the cyclic peptide; a second step of calculating a molecular shape factor r which is calculated by Expression (1) after a step of carrying out an ellipsoidal approximation for obtaining each of axis lengths a, b, and c in a case where an axis length in a longest axis direction of a main chain structure is denoted by a, and axis lengths in two other directions which are orthogonal to a and are orthogonal to each other are denoted by b and c in the structure acquired in the first step; and a third step of determining that the cyclic peptide having the molecular shape factor r in a range of 0.4 to 0.6 has cell membrane permeability. r=2b2+c2a2+b2+c2+a2

The invention relates to a method for increasing folic acid content of wheat malt seedlings through ultrasonic field and amino acid solution treatment, which belongs to the technical field of agricultural food biology and is characterized in that wheat grains with high germination rate are used as raw materials, and the wheat malt seedlings are cultivated through soaking disinfection, amino acid solution dipping and ultrasonic field treatment and regulation and control of illumination, temperature, humidity and CO2 concentration. And drying and crushing to obtain the wheat malt seedling powder rich in folic acid. According to the method disclosed by the invention, the permeability of cell membranes is enhanced by utilizing an ultrasonic cavitation effect, folic acid precursor absorption and synthetase activation are promoted, the folic acid content in the wheat malt seedlings is effectively increased, and the wheat malt seedling powder with the folic acid content of 450-550mu g / 100g is obtained.

The present invention provides a cyclic peptide compound optionally linked to a nucleic acid, the cyclic peptide compound having a cyclic moiety wherein: (1) the cyclic moiety is composed of 13 or 14 amino acid residues; (2) among the amino acid residues constituting the cyclic moiety, the number of N-substituted amino acid residues is 7 or more, the number of amino acid residues in a single side chain is 3 or fewer, and the number of native amino acid residues is 4 or fewer; and (3) the ClogP / total amide bond (ClogP / total AB) is 1.0 to 1.8.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Z34B3 and application thereof. The amino acid sequence of the antibacterial peptide Z34B3 provided by the invention is shown as SEQ ID NO: 1, the secondary structure is an alpha helix, the molecular weight is small, and the antibacterial peptide Z34B3 can change the cell membrane permeability of various gram-negative bacteria including pasteurella multocida, escherichia coli and acinetobacter baumannii, inhibit the generation of cell membranes and play antibacterial and bactericidal roles. Moreover, the antibacterial peptide Z34B3 provided by the invention has low hemolytic activity and no cytotoxicity, and can be applied to preparation of products with bacteriostatic and bactericidal effects and preparation of drugs for preventing and / or treating bacterial infectious diseases. Furthermore, the C tail end of the antibacterial peptide Z34B3 is subjected to amidation modification, and compared with non-modification treatment, the stability can be improved.

The application discloses a kind of strong electron donor triphenylamine modified coumarin dyes, its synthesis method and application;The dye has blue-green light excitation characteristics, the maximum absorption wavelength is 415-425 nm, the maximum emission wavelength is 495-505 nm, and excellent lysosome targeting and positioning ability is shown.It overcomes the problem of low efficiency of traditional coumarin as photosensitizer in molecular structure design, realizes the efficient I type photodynamic effect, can generate active oxygen under light efficiently.In addition, this dye has good cell membrane permeability and low dark toxicity, suitable for biomedical imaging, photodynamic therapy of tumor and other fields.The synthesis method is simple, raw materials are easy to obtain, and the yield is high, suitable for large-scale production.Experiments show that the dye can effectively enrich in cell lysosomes, realize precise killing of cancer cells, and show great potential in cancer diagnosis and treatment integration.

This invention discloses a method and apparatus for preserving fresh Chinese medicinal herbs using a combined electric and magnetic field, belonging to the field of post-harvest preservation technology. The method includes: placing cleaned and disinfected fresh Chinese medicinal herbs in a storage device, while simultaneously applying a low-frequency polarized electric field and a static magnetic field; wherein the output voltage of the low-frequency polarized electric field is 3000 V, the current is 1-5 mA, the frequency is 50-60 Hz, and the electric field strength is 25-35 kV / m; the strength of the static magnetic field is 350-450 Gs. This invention effectively regulates cell membrane permeability and free radical metabolism through the spatial synergistic effect of the low-frequency polarized electric field and the static magnetic field, significantly delaying post-harvest decay of Chinese medicinal herbs, inhibiting microbial growth, maintaining the stability of active ingredients, and achieving good preservation effects even at relatively high storage temperatures. It has advantages such as energy saving, being green, easy to operate, and highly applicable, and is suitable for the storage and preservation of various fresh Chinese medicinal herbs.

A photoredox molecular swimmer, structurally comprising a photoredox center, a redox shuttle, and a flexible PEG chain covalently linking the photoredox center and the redox shuttle. The photoredox molecular swimmer of the present invention enhances cell membrane permeability, promotes entry of extracellular chemicals into living cells, and induces on-demand apoptosis.

This invention discloses a two-photon fluorescent dye, prepared as follows: 6-hydroxy-2-naphthaldehyde reacts with a dihaloalkane under alkaline conditions to obtain a monohaloalkane-based aryl ether, which is then reacted with an aqueous trimethylamine solution to prepare S1; diethyl 4-(4-tolyl)pyridine-2,6-dicarboxylate reacts with N-bromosuccinimide to obtain a brominated product, which is then reacted with triphenylphosphine to prepare S2. After intermediates S1 and S2 react under strongly alkaline conditions, the product is then hydrolyzed to prepare the target product. This dye exhibits good cell membrane permeability, good dispersion in the cytoplasm, preferentially targets organelles in the cytoplasm, is water-soluble, has very low toxicity, and a high two-photon absorption interface, making it suitable for two-photon fluorescence confocal microscopy imaging.

The application discloses a curcuminoid xanthene compound shown in formula I, a preparation method of the curcuminoid xanthene compound and application of the curcuminoid xanthene compound as a photodiagnosis and treatment agent. The compound has the characteristics of long absorption and emission wavelength, good stability, high phototoxicity, large Stokes shift, good biocompatibility and good cell membrane permeability. Compared with curcumin, the photosensitizer has a longer absorption wavelength, lower dark toxicity and can generate ROS under type-I and type-II synergistic action, and is expected to be applied to PDT in an anaerobic tumor microenvironment.

The present invention provides a cyclic peptide compound optionally linked to a nucleic acid, wherein the cyclic peptide compound contains a cyclic portion, (1) the cyclic portion is composed of 13 or 14 amino acid residues, (2) among the amino acid residues composing the cyclic portion, the number of N-substituted amino acid residues is 7 or more, the number of amino acid residues having the same side chain is 3 or less, and the number of natural amino acid residues is 4 or less, and (3) ClogP / total amide bond (ClogP / total AB) is 1.0 to 1.8.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to medicinal ink for accelerating lung tumor cell apoptosis and a preparation method and application thereof. Different raw materials are subjected to esterification modification, fermentation or enzymolysis, so that the fat solubility and cell membrane permeability of the composition are promoted, active ingredients can penetrate through a qi and blood barrier more easily to enter lung tissues, the action time is prolonged, the targeting property of the composition is improved, and the conversion of chemical components of traditional Chinese medicines is promoted; a new substance with higher anti-cancer activity is generated or obtained; the bioavailability of each raw material is improved, and a specific enzymolysis product is generated, so that the apoptosis of tumor cells is accelerated. Therefore, different raw materials are specifically prepared, release of active functions of the raw materials is guaranteed from multiple dimensions, and lung tumor cell apoptosis is remarkably accelerated.

The application discloses a method for preventing low-temperature freezing injury of sugarcane seedlings and relates to the technical field of sugarcane planting. The method for preventing freezing injury is that Bacillus amyloliquefaciens is uniformly mixed with base fertilizer and is applied together in the process of sugarcane planting, and a freezing prevention agent is sprayed in the sugarcane seedling stage, wherein the freezing prevention agent comprises 1-5 parts of sodium chloride, 100-200 parts of wheat extract and 2-10 parts of sodium caseinate according to weight fractions. The method for preventing freezing injury can increase the proline content in the cells of the sugarcane, reduce the cell membrane permeability, increase the osmotic pressure in the cells, prevent protoplasm from dehydrating and coagulating, and also increase the CAT, SOD and POD enzyme activities, so that the oxidation damage of oxygen negative ions to the cells and tissues is prevented, and the purpose of relieving the freezing injury of leaf cells is achieved.

The invention discloses a micromolecular fluorescent probe for specific imaging autophagy and a preparation method and application thereof, the fluorescent probe comprises a fluorophore, a linking group and an autophagy targeting group, the fluorophore is an intelligent fluorescence reporter group, the linking group adopts cysteine as a linking unit, and the autophagy targeting group targets LC3 protein; the probe can be used for specifically imaging an autophagy process of a biological sample and screening an autophagy regulator. The micromolecular fluorescent probe has the advantages of being good in cell membrane permeability, simple and convenient in dyeing process and easy to be compatible with other fluorescence labeling technologies, can perform specific recognition and selective imaging on an autophagy related structure, improves the targeting property and imaging credibility of the probe in a complex cell environment, and can be used for detecting autophagy related structures. The method can be used for visual tracing and detection of autophagy flow in living cells under physiological and pathological conditions, and is beneficial for promoting a small-molecule fluorescent probe to analyze an autophagy regulation mechanism and biological functions in real time.

The invention provides a real-time monitoring method and system for the sterilization effect of an aldehyde disinfectant, and relates to the technical field of process monitoring, and the method comprises the steps: collecting a microbial electrochemical response signal, and obtaining a time domain impedance module value, a phase angle and an environmental parameter time sequence. Wavelet packet decomposition is carried out on the impedance module value time sequence, cell membrane permeability characteristic frequency band signals are extracted, and a frequency domain envelope spectrum time sequence is generated. Four types of time sequences are aligned to form a three-dimensional feature tensor, and a sterilization efficiency feature vector is generated through gating attention fusion. Constructing a frequency domain relation graph, calculating an edge weight based on spectrum similarity, and obtaining a frequency domain enhancement matrix through double-layer graph volume accumulation; and by taking the time domain feature as a query and the frequency domain enhancement feature as a key value, injecting a biological constraint matrix to correct the attention weight, and fusing the time-frequency feature. And through bidirectional LSTM coding, the sterilization efficiency, the inactivation logarithm and the microorganism load prediction value are output in real time. The method combines electrochemical analysis and a deep learning technology, realizes rapid and accurate evaluation of the disinfection effect, and is suitable for medical disinfection monitoring scenes.

The invention discloses a conductivity-paper roll method-based method for rapidly identifying the vigor of plum-leaf crab seeds, and relates to the technical field of plant cultivation, and the method comprises the following steps: carrying out seed sampling, cleaning and moisture content balance treatment; measuring the conductivity of the seed leachate and judging the integrity of a cell membrane; culturing germination by adopting a wet paper roll method, and calculating a comprehensive vitality index; and comprehensively judging a batch release result according to a threshold value and a rechecking mechanism. According to the method, the cell membrane permeability is represented through the conductivity, the germination potential is reflected through the germination rate, a quantitative threshold value is established by combining double indexes, rapid grading of the seed vigor is achieved, compared with a traditional germination method, the detection period is shortened from 28 days to 14 days or less, operation is easy and convenient, chemical dyes are not needed, the result is traceable, repeatability is high, and the method is suitable for large-scale popularization and application. The method can be widely applied to activity detection, batch release and quality control of the plum-leaf crab and other fruit tree seeds.

A microwave acarus killing instrument comprises a machine body, a microwave emitting device used for emitting microwaves to a cleaning face is arranged on the machine body, the emitting end of the microwave emitting device is located at the bottom of the machine body, a control device used for controlling the microwave emitting device to work is arranged on the machine body, and the control device is electrically connected with the microwave emitting device. The microwave emitting device used for emitting microwaves is arranged on the machine body, mites are removed through the microwaves, due to the dual effects of the heat effect and the non-heat effect generated by the microwaves, the two effects are synergistic, mites and eggs of the mites can be rapidly and thoroughly removed, and no chemical residues exist; the heat effect of microwaves is as follows: the microwaves enable polar molecules such as water molecules in acarid bodies to vibrate and rub at a high speed, high temperature is generated instantly, proteins of the acarid are denatured, biological activity is lost, and the acarid dies; according to the non-thermal effect of microwaves, a high-frequency electromagnetic field directly acts on cell membrane potentials and genetic materials of mites, normal physiological activities of the mites are interfered, cell membrane permeability is changed, and nutrient imbalance is caused.

The invention belongs to the technical field of antibacterial active peptides, and particularly relates to an antibacterial peptide and application thereof in preparation of antibacterial drugs. The antibacterial peptide is not easy to generate drug resistance and is the most promising antibiotic substitute. The invention provides antibacterial peptide-1-antibacterial peptide-20 and a derivative sequence thereof, the sequence of the antibacterial peptide-1-antibacterial peptide-20 is shown as SEQ ID NO.1-19, and the antibacterial peptide-1-antibacterial peptide-20 can show better antibacterial (bacteria or fungi) activity and can be applied to treatment of infectious diseases caused by bacteria or fungi. The factors such as hemolytic activity, cytotoxicity, cell membrane permeability and cell drug resistance are combined for investigation, and the antibacterial peptides such as the derivatives of the antibacterial peptide-2 and the antibacterial peptide-20 have better antibacterial activity on bacteria and fungi and are expected to be applied to development of clinical antibacterial drugs.

The invention provides a cyclic peptide permeability prediction method and system based on multi-modal contrast learning, and relates to the technical field of biological information and computer-aided drug design, and the method comprises the steps: obtaining one-dimensional sequence information, two-dimensional topological structure information and three-dimensional space conformation information of a to-be-predicted cyclic peptide; parallel feature coding is carried out on the three modes, and sequence-level features, two-dimensional topological features and three-dimensional geometrical feature embedding are generated respectively; optimizing the sequence-level feature embedding and the two-dimensional topological feature embedding by adopting a double contrast learning strategy to obtain optimized sequence-level feature embedding and optimized two-dimensional topological feature embedding; and performing feature fusion on the optimized sequence-level feature embedding, the optimized two-dimensional topological feature embedding and the three-dimensional geometrical feature embedding to obtain a multi-modal feature vector, and inputting the multi-modal feature vector into a multi-layer perceptron classifier to obtain a binary classification prediction result of whether the cyclopeptide has the cell membrane permeability or not. According to the method, the accuracy, robustness and interpretability of predicting the permeability of the cyclopeptide are remarkably improved.

This invention provides a coumarin-chalcone hybrid compound, its preparation method, and its applications. The structure of the coumarin-chalcone hybrid compound is shown in Formula I. The coumarin-chalcone hybrid compound provided by this invention has a simple and easy synthesis process, readily available raw materials, and can significantly activate the transcriptional activity of Nrf2 at both the cellular and animal levels. It exhibits high safety, good cell membrane permeability, and can be taken orally. After oral administration, it primarily results in high exposure in the liver and can effectively alleviate liver damage caused by acetaminophen and alcohol.

The invention relates to the technical field of nano materials, and discloses a self-driven low-oxygen responsive Z-DNA targeted Janus nano motor and a preparation method thereof.The preparation method comprises the steps that firstly, an ammonia water solution and absolute ethyl alcohol are mixed and react with tetraethoxysilane to obtain a first solution, and the first solution is freeze-dried to obtain spherical MSN nano particles; preparing a chitosan solution; a second solution is obtained from the spherical MSN nano particles and gamma-glycidyl ether oxypropyl trimethoxy silane; adding a chitosan solution, chloroquine phosphate, anhydrous copper chloride and a hydrogen peroxide solution to obtain a third solution; adding an azo compound, freeze-drying, dispersing the obtained particles in absolute ethyl alcohol, dropwise adding on the surface of a silicon wafer to form a uniform film layer, and forming a platinum thin layer by using an ion sputtering instrument to obtain a nano motor CP-Chl (at) MPt; the problems that in the prior art, a targeting mechanism is lacked, a biological membrane cannot be actively penetrated, targeted intervention Z-DNA structure is lacked, the synergistic effect of ROS on cell membrane permeability and drug internalization is neglected, and the sterilization efficiency of an infected part is not high are solved.

The invention discloses a method for promoting recombinant corynebacterium glutamicum to secrete chondroitin oligosaccharide by changing cell membrane permeability, and belongs to the field of fermentation engineering. According to the invention, a high-copy double-plasmid system is used in corynebacterium glutamicum for coupling synthesis and cutting of chondroitin through a genetic engineering means, so that the yield of chondroitin oligosaccharide synthesized by corynebacterium glutamicum is increased. In order to promote the secretion of chondroitin oligosaccharide, the permeability of cell membranes is increased by quantitatively and regularly adding a surfactant tween-40 in the fermentation process. Through high-copy plasmid expression, culture medium optimization, surfactant treatment and other strategies, the yield of chondroitin oligosaccharide generated by fermentation of the constructed recombinant corynebacterium glutamicum is increased by 37.5%.

This invention relates to the field of silicone rubber technology and discloses a preparation process for an antibacterial modified silicone rubber composite material. The invention involves mixing methyl vinyl silicone rubber, organosilicon quaternary ammonium salt polyimide, reinforcing agents, accelerators, antioxidants, crosslinking agents, and vulcanizing agents in an open mill, followed by two-stage vulcanization in a flat vulcanizing mill to obtain the antibacterial modified silicone rubber composite material. The organosilicon quaternary ammonium salt polyimide of this invention exhibits strong high-temperature resistance and resistance to thermal decomposition, which can increase the thermal decomposition mass loss temperature of silicone rubber materials and improve their high-temperature resistance. Simultaneously, the organosilicon quaternary ammonium salt polyimide contains quaternary ammonium salt antibacterial groups, which can interact electrostatically with the negatively charged cell membranes of bacteria, altering cell membrane permeability and allowing substances within the cell membrane to flow out, thereby inhibiting and killing bacteria and significantly improving the antibacterial properties of the silicone rubber material.

The invention discloses peptidoglycan hydrolase as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria, including bacteriostasis and sterilization. The invention discloses application of peptidoglycan hydrolase, and particularly discloses application of peptidoglycan hydrolase as well as pharmaceutical salt or derivative thereof. The application is any one of the following items: A1) application in preparation of bacterial lysis products; a2) is applied to preparation of peptidoglycan products from lysis bacteria; a3) application in preparation of a product for inhibiting bacterial growth; a4) in the preparation of a product for inhibiting the formation of a bacterial biofilm; a5) application in preparation of a product for enhancing the sensitivity of bacteria to antibiotics; and A6) application in preparation of a sterilization product combined with a cell membrane permeation accelerant. A7) application in preparation of a product combined with a cell membrane permeability accelerant to disintegrate a biofilm; and A8) application in preparation of sterilization products. The protein is any one of the following items: B1) a protein with an amino acid sequence being a sequence 2 or a sequence 4; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the peptidoglycan hydrolase in B1), has 80% or more of identity with the protein shown in B1) and has the same activity with the protein shown in B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The protein disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

The invention discloses a ratio type near-infrared fluorescent probe Dn-NIR-Sec of selenocysteine as well as a preparation method and application of the ratio type near-infrared fluorescent probe Dn-NIR-Sec. The chemical structural formula of the ratio type near-infrared fluorescent probe Dn-NIR-Sec is shown as a formula (I). The preparation method comprises the following steps: condensing a compound 1 shown as a structural formula (1) with cyclohexanone under an acidic condition to obtain a compound 2 shown as a structural formula (2); the preparation method comprises the following steps: firstly, preparing a compound 2, then condensing the compound 2 and a compound 3 with a structural formula as shown in a formula (3) to obtain a ratio-type near-infrared dye platform Dn-NIR-OH, and finally reacting with 2, 4-dinitrobenzenesulfonyl chloride to obtain the ratio-type near-infrared dye platform Dn-NIR-OH. Experimental results show that the ratio-type near-infrared fluorescent probe Dn-NIR-Sec can complete rapid fluorescent response to Sec within 5 minutes, real-time dynamic detection is facilitated, selective recognition performance on selenocysteine is remarkable, light stability is good, and meanwhile the ratio-type near-infrared fluorescent probe Dn-NIR-Sec has good cell membrane permeability and low cytotoxicity. Therefore, the probe can be widely applied to the fields of biological detection and the like as a fluorescence detection tool.

The present application is a kind of aldehyde disinfectant sterilization effect real-time monitoring method and system, relating to process monitoring technical field, including collecting microorganism electrochemical response signal, obtaining time domain impedance modulus, phase angle and environment parameter time sequence.Wavelet packet decomposition is carried out on impedance modulus time sequence, cell membrane permeability characteristic frequency band signal is extracted, and frequency domain envelope spectrum time sequence is generated.Four types of time sequence are aligned to form three-dimensional feature tensor, and sterilization efficiency feature vector is generated through gated attention fusion.Frequency domain relationship diagram is constructed, edge weight is calculated based on frequency spectrum similarity, and frequency domain enhancement matrix is aggregated through double-layer graph convolution.The time domain features are used as queries, and the frequency domain enhancement features are used as key values, and the biological constraint matrix is injected to modify the attention weight, and the time-frequency features are fused.The sterilization efficiency, inactivation logarithm and microorganism load prediction value are output in real time through bidirectional LSTM coding.The method combines electrochemical analysis and deep learning technology to realize fast and accurate evaluation of disinfection effect, and is suitable for medical disinfection monitoring scene.