123 results about "Cell membrane permeability" patented technology

The invention relates to the field of computer-aided drug design (CADD) and molecular informatics, in particular to a multi-modal representation learning method based on cyclopeptide molecules, which is used for predicting cell membrane permeability of cyclopeptide. The method mainly comprises the following steps: (1) data collection: integrating cyclic peptide permeability data from a ChEMBL database, a CycPeptMPDB database and a CyclicPepeda database and patent literatures; (2) multi-modal learning: for different modal data, a deep learning model is adopted to extract feature representations of the data; the method comprises the following steps of: encoding an SMILES sequence by using ChemBERTa (ChemBERTa); using Vision Transform to extract molecular image features, and learning a molecular image structure and 3D coordinate information based on GNN; (3) multi-modal feature fusion: adopting a self-adaptive extensible fusion mechanism, integrating SMILES feature information into image, graph and 3D coordinate features through a cross-modal feature fusion mechanism, and splicing all modal features to obtain multi-modal molecular representation; and (4) permeability prediction: sending the multi-modal molecular representation into a full connection layer for regression prediction so as to evaluate the permeability of the cyclopeptide.

The invention discloses a device and system for conducting electric field treatment on the digestive tract, the device for conducting electric field treatment on the digestive tract comprises a slender catheter, a supporting body located at the far end of the catheter, an electrode array film and a pulse generator, the electrode array film and the pulse generator are arranged on the supporting body, and the output end of the pulse generator is coupled with an electrode pair on the electrode array film. A sequence pulse wave output by the pulse generator comprises a plurality of groups of nanosecond pulse strings, and the time interval between the adjacent nanosecond pulse strings is 1us-10000 us; the nanosecond pulse string is composed of a plurality of nanosecond pulse pairs, and the time interval between every two adjacent nanosecond pulse pairs ranges from 50 ns to 10000 ns; the nanosecond pulse pair comprises positive pulses and negative pulses which appear alternately, the pulse width of the positive pulses and the pulse width of the negative pulses are 10-1000 ns, and the time interval is 10-1000 ns. The cell membrane permeability and the cell death efficiency can be remarkably improved, and the limitation of the traditional IRE technology is broken through.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a 2, 4-disubstituted quinazoline derivative as well as a preparation method and application thereof. According to the invention, a series of 2, 4-disubstituted quinazoline derivative micromolecular ligands capable of acting on telomere G4 are developed on the basis of a quinazoline skeleton. The 2, 4-disubstituted quinazoline derivative disclosed by the invention has certain telomere G4 stability and telomere G4 binding capacity, better anti-tumor cell proliferation activity, low toxicity, higher cell membrane permeability and certain oral bioavailability, in addition, the 2, 4-disubstituted quinazoline derivative disclosed by the invention can activate congenital immune response of tumor cells, and can be used for preparing anti-tumor drugs. The compound has a wide prospect when being applied to preparation of anti-tumor drugs.

The invention relates to the technical field of medical biology, and discloses a composition for promoting interaction of collagen and hyaluronic acid, which comprises collagen, hyaluronic acid and a chemical cross-linking agent. According to the preparation method of the composition for promoting interaction of the collagen and the hyaluronic acid and the application of the chemical cross-linking agent N (beta maleimido) propionate, a stable chemical bond is formed between the collagen and the hyaluronic acid, and the molecular structure is optimized, so that the composition is easier to penetrate through the cuticle of the skin, and the interaction of the collagen and the hyaluronic acid is promoted. Physical treatment of ultrasonic waves and a high-voltage pulse electric field accelerates formation of a compound, the skin barrier is reduced through mechanical and electroporation effects, conditions are provided for deep penetration of the compound, particles are refined through the cavitation effect of the ultrasonic waves, and uniformity and fluidity are improved; the electroporation effect of the high-voltage pulsed electric field increases the permeability of cell membranes, an additional permeation path is provided for the compound, and the comprehensive measures effectively improve the transdermal absorption efficiency and the biological activity of the compound.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

The invention relates to a B cell specific light chain detection kit, and belongs to the technical field of biology. The B cell specific light chain detection kit comprises an antibody composition, a fixing agent and a permeabilized buffer solution, the antibody composition comprises an A group antibody and a B group antibody, wherein the A group antibody comprises an anti-Kappa antibody and an anti-Lambda antibody; the group B antibody comprises an anti-CD19 antibody, an anti-CD38 antibody, an anti-CD5 antibody, an anti-CD10 antibody, an anti-CD20 antibody and an anti-CD45 antibody; the permeabilization buffer solution comprises amino acid modified saponin. And the contained permeabilized buffer solution is modified by amino acid, so that the permeability of cell membranes can be ensured, the cell morphology cannot be excessively damaged, and the accuracy of B cell specific light chain detection is improved.

Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

The present disclosure provides a carrier peptide fragment with excellent cell membrane permeability. The carrier peptide fragment disclosed herein includes the following amino acid sequence set forth in SEQ ID No.: 1: KKRTLRKSNRKKR, and a lysine residue at a 12th position from an N-terminus of the amino acid sequence is a D-form, and the other amino acid residues are L-forms.

The present invention discloses a polymer for target protein hydrolysis, a preparation method and a use thereof. The polymer of the present invention is combined with a target protein ligand and an E3 ligase ligand, and the polymer can solve the delivery problems existing in the existing delivery system in terms of water solubility, pharmacokinetic properties and cell membrane permeability. At the same time, the polymer prepared by the present invention can be assembled to form nanoparticles, which have natural targeting in vivo. In addition, the polymer of the present invention is a "multi-linker" type molecule, and the number and ratio of the side chain ligand molecules that bind to the target protein and the ligand molecules that recruit the E3 ligase can be flexibly adjusted, which is conducive to the construction of a combinatorial library for optimization, thereby obtaining the best protein degradation efficiency.

The present invention addresses the problem of providing a novel synthetic peptide having cell membrane permeability. [Solution] A synthetic peptide disclosed by the present invention comprises any one of the following amino acid sequences: (1) an amino acid sequence in which two or more minimum structural units are continuously bonded in series, said minimum structural units having GD (glycine residue-aspartic acid residue) or GE (glycine residue-glutamic acid residue) as the minimum structural units; and (2) an amino acid sequence obtained by binding 1-3 glycine residues to the C-terminal side of the amino acid sequence of (1).

An object of the present invention is to provide a method for predicting cell membrane permeability of a cyclic peptide, which enables versatile design of a cyclic peptide with cell membrane permeability. According to the present invention, there is provided a method for predicting cell membrane permeability of a cyclic peptide, the method including a first step of acquiring a structure of the cyclic peptide; a second step of calculating a molecular shape factor r which is calculated by Expression (1) after a step of carrying out an ellipsoidal approximation for obtaining each of axis lengths a, b, and c in a case where an axis length in a longest axis direction of a main chain structure is denoted by a, and axis lengths in two other directions which are orthogonal to a and are orthogonal to each other are denoted by b and c in the structure acquired in the first step; and a third step of determining that the cyclic peptide having the molecular shape factor r in a range of 0.4 to 0.6 has cell membrane permeability. r=2b2+c2a2+b2+c2+a2

Provided are: a nucleic acid for transfection having excellent cell membrane permeability; a method for producing the nucleic acid for transfection; and a transfection method. The present invention is a nucleic acid for transfection in which a target nucleic acid introduced into a cell and a cell membrane-permeable group are linked, the cell membrane-permeable group containing a perfluoroalkyl group having 2-10 carbon atoms, the perfluoroalkyl group optionally having 1-5 ether-linkable oxygen atoms between carbon atoms, and a method for transfecting a nucleic acid, the method for transfecting the nucleic acid involves bringing the nucleic acid for transfection into contact with a cell and introducing the nucleic acid into the cell.

The invention relates to a method for increasing folic acid content of wheat malt seedlings through ultrasonic field and amino acid solution treatment, which belongs to the technical field of agricultural food biology and is characterized in that wheat grains with high germination rate are used as raw materials, and the wheat malt seedlings are cultivated through soaking disinfection, amino acid solution dipping and ultrasonic field treatment and regulation and control of illumination, temperature, humidity and CO2 concentration. And drying and crushing to obtain the wheat malt seedling powder rich in folic acid. According to the method disclosed by the invention, the permeability of cell membranes is enhanced by utilizing an ultrasonic cavitation effect, folic acid precursor absorption and synthetase activation are promoted, the folic acid content in the wheat malt seedlings is effectively increased, and the wheat malt seedling powder with the folic acid content of 450-550mu g / 100g is obtained.

The present invention provides a cyclic peptide compound optionally linked to a nucleic acid, the cyclic peptide compound having a cyclic moiety wherein: (1) the cyclic moiety is composed of 13 or 14 amino acid residues; (2) among the amino acid residues constituting the cyclic moiety, the number of N-substituted amino acid residues is 7 or more, the number of amino acid residues in a single side chain is 3 or fewer, and the number of native amino acid residues is 4 or fewer; and (3) the ClogP / total amide bond (ClogP / total AB) is 1.0 to 1.8.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Z34B3 and application thereof. The amino acid sequence of the antibacterial peptide Z34B3 provided by the invention is shown as SEQ ID NO: 1, the secondary structure is an alpha helix, the molecular weight is small, and the antibacterial peptide Z34B3 can change the cell membrane permeability of various gram-negative bacteria including pasteurella multocida, escherichia coli and acinetobacter baumannii, inhibit the generation of cell membranes and play antibacterial and bactericidal roles. Moreover, the antibacterial peptide Z34B3 provided by the invention has low hemolytic activity and no cytotoxicity, and can be applied to preparation of products with bacteriostatic and bactericidal effects and preparation of drugs for preventing and / or treating bacterial infectious diseases. Furthermore, the C tail end of the antibacterial peptide Z34B3 is subjected to amidation modification, and compared with non-modification treatment, the stability can be improved.

The application discloses a kind of strong electron donor triphenylamine modified coumarin dyes, its synthesis method and application;The dye has blue-green light excitation characteristics, the maximum absorption wavelength is 415-425 nm, the maximum emission wavelength is 495-505 nm, and excellent lysosome targeting and positioning ability is shown.It overcomes the problem of low efficiency of traditional coumarin as photosensitizer in molecular structure design, realizes the efficient I type photodynamic effect, can generate active oxygen under light efficiently.In addition, this dye has good cell membrane permeability and low dark toxicity, suitable for biomedical imaging, photodynamic therapy of tumor and other fields.The synthesis method is simple, raw materials are easy to obtain, and the yield is high, suitable for large-scale production.Experiments show that the dye can effectively enrich in cell lysosomes, realize precise killing of cancer cells, and show great potential in cancer diagnosis and treatment integration.

This invention discloses a method and apparatus for preserving fresh Chinese medicinal herbs using a combined electric and magnetic field, belonging to the field of post-harvest preservation technology. The method includes: placing cleaned and disinfected fresh Chinese medicinal herbs in a storage device, while simultaneously applying a low-frequency polarized electric field and a static magnetic field; wherein the output voltage of the low-frequency polarized electric field is 3000 V, the current is 1-5 mA, the frequency is 50-60 Hz, and the electric field strength is 25-35 kV / m; the strength of the static magnetic field is 350-450 Gs. This invention effectively regulates cell membrane permeability and free radical metabolism through the spatial synergistic effect of the low-frequency polarized electric field and the static magnetic field, significantly delaying post-harvest decay of Chinese medicinal herbs, inhibiting microbial growth, maintaining the stability of active ingredients, and achieving good preservation effects even at relatively high storage temperatures. It has advantages such as energy saving, being green, easy to operate, and highly applicable, and is suitable for the storage and preservation of various fresh Chinese medicinal herbs.

A photoredox molecular swimmer, structurally comprising a photoredox center, a redox shuttle, and a flexible PEG chain covalently linking the photoredox center and the redox shuttle. The photoredox molecular swimmer of the present invention enhances cell membrane permeability, promotes entry of extracellular chemicals into living cells, and induces on-demand apoptosis.

A cyclic peptide or a salt thereof having excellent cell membrane permeability, and an MIDMX inhibitor are provided. The cyclic peptide or a salt thereof is represented by Formula (1) in the specification and has characteristics (a) to (c): (a) In a structure of the cyclic peptide, where an axis length in a longest axis direction of a main chain structure is denoted by a, and axis lengths in two other directions which are orthogonal to a and are orthogonal to each other are denoted by b and c, a molecular shape factor r calculated by Formula (2) after a step of carrying out an ellipsoidal approximation for obtaining each of the axis lengths a, b, and c is within a range of 0.4 to 0.6; (b) the main chain structure of the peptide contains a sulfur atom; and (c) the peptide is non-ionic in a physiological environment:r=2⁢b2+c2a2+b2+c2+a2.(2)

A cyclic peptide or a salt thereof having excellent cell membrane permeability and target binding ability to MDMX, and an MDMX inhibitor are provided. The peptide is represented by Formula (1) in the specification and has characteristics (a) to (d): (a) In a structure of the peptide, where an axis length in a longest axis direction of a main chain structure is denoted by a, and axis lengths in two other directions which are orthogonal to a and to each other are denoted by b and c, a molecular shape factor r calculated by Formula (2) after a step of carrying out an ellipsoidal approximation for obtaining each of the axis lengths a, b, and c is within a range of 0.4 to 0.6; (b) the peptide is non-ionic in a physiological environment; (c) a proportion of the number of N-substituted amino acids with respect to the total number of amino acids of the peptide is 25% or more; and (d) the peptide contains 1 to 3 amino acid residues and amino acid analog residues each having, in a side chain, an aromatic carbocyclic group or an aromatic heterocyclic group:r=2⁢b2+c2a2+b2+c2+a2.(2)

This invention discloses a two-photon fluorescent dye, prepared as follows: 6-hydroxy-2-naphthaldehyde reacts with a dihaloalkane under alkaline conditions to obtain a monohaloalkane-based aryl ether, which is then reacted with an aqueous trimethylamine solution to prepare S1; diethyl 4-(4-tolyl)pyridine-2,6-dicarboxylate reacts with N-bromosuccinimide to obtain a brominated product, which is then reacted with triphenylphosphine to prepare S2. After intermediates S1 and S2 react under strongly alkaline conditions, the product is then hydrolyzed to prepare the target product. This dye exhibits good cell membrane permeability, good dispersion in the cytoplasm, preferentially targets organelles in the cytoplasm, is water-soluble, has very low toxicity, and a high two-photon absorption interface, making it suitable for two-photon fluorescence confocal microscopy imaging.

The invention belongs to the technical field of antibacterial materials, and particularly relates to a composite nano material as well as a preparation method and application thereof. The composite nano material comprises modified mesoporous polydopamine coated with a hyaluronic acid membrane and gold modified on the surface of the hyaluronic acid membrane, the modified mesoporous polydopamine is prepared from copper ion chelated mesoporous polydopamine and BNN6 loaded on the copper ion chelated mesoporous polydopamine. Gold and mesoporous polydopamine in the composite nano material can absorb near-infrared light and convert the near-infrared light into heat under the irradiation of the near-infrared light, BNN6 is activated and can release NO gas harmful to fungi or bacteria along with temperature rise, and the NO gas can interfere the metabolic process of microorganisms, change the permeability of cell membranes, damage the cell membranes and inhibit the growth of the fungi or the bacteria, so that the growth of the fungi or the bacteria is inhibited. And even death is caused. According to the invention, NO gas and an inorganic antibacterial agent are jointly applied to microorganism resistance, and the antibacterial effect is good.

The application discloses a curcuminoid xanthene compound shown in formula I, a preparation method of the curcuminoid xanthene compound and application of the curcuminoid xanthene compound as a photodiagnosis and treatment agent. The compound has the characteristics of long absorption and emission wavelength, good stability, high phototoxicity, large Stokes shift, good biocompatibility and good cell membrane permeability. Compared with curcumin, the photosensitizer has a longer absorption wavelength, lower dark toxicity and can generate ROS under type-I and type-II synergistic action, and is expected to be applied to PDT in an anaerobic tumor microenvironment.

The present invention provides a cyclic peptide compound optionally linked to a nucleic acid, wherein the cyclic peptide compound contains a cyclic portion, (1) the cyclic portion is composed of 13 or 14 amino acid residues, (2) among the amino acid residues composing the cyclic portion, the number of N-substituted amino acid residues is 7 or more, the number of amino acid residues having the same side chain is 3 or less, and the number of natural amino acid residues is 4 or less, and (3) ClogP / total amide bond (ClogP / total AB) is 1.0 to 1.8.

The invention relates to an application of a plant secondary metabolite in inhibiting pathogenicity of fusarium oxysporum. The application comprises the step of realizing an antibacterial effect through at least one mechanism of inhibiting hypha growth, spore germination, cell membrane permeability change, defensive enzyme activity reduction, cell wall degrading enzyme activity inhibition or toxin synthesis reduction of pathogenic bacteria. The antibacterial action mode of the 2, 4-di-tert-butylphenol is that the 2, 4-di-tert-butylphenol can destroy the completeness of cell membranes of fusarium oxysporum of cucumbers and disturb the functions of the cell membranes to cause leakage of cell contents and oxidative damage, so that normal physiological activities of cells are inhibited. Meanwhile, 2, 4-di-tert-butylphenol can inhibit defensive enzyme activity and cell wall degrading enzyme activity of fusarium oxysporum and has a remarkable inhibition effect on toxin synthesis, so that the purpose of inhibiting fusarium oxysporum hyphae from infecting host plants is achieved, and a basis is provided for subsequent deeper research on a fusarium oxysporum inhibition mechanism.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to medicinal ink for accelerating lung tumor cell apoptosis and a preparation method and application thereof. Different raw materials are subjected to esterification modification, fermentation or enzymolysis, so that the fat solubility and cell membrane permeability of the composition are promoted, active ingredients can penetrate through a qi and blood barrier more easily to enter lung tissues, the action time is prolonged, the targeting property of the composition is improved, and the conversion of chemical components of traditional Chinese medicines is promoted; a new substance with higher anti-cancer activity is generated or obtained; the bioavailability of each raw material is improved, and a specific enzymolysis product is generated, so that the apoptosis of tumor cells is accelerated. Therefore, different raw materials are specifically prepared, release of active functions of the raw materials is guaranteed from multiple dimensions, and lung tumor cell apoptosis is remarkably accelerated.

The application discloses a method for preventing low-temperature freezing injury of sugarcane seedlings and relates to the technical field of sugarcane planting. The method for preventing freezing injury is that Bacillus amyloliquefaciens is uniformly mixed with base fertilizer and is applied together in the process of sugarcane planting, and a freezing prevention agent is sprayed in the sugarcane seedling stage, wherein the freezing prevention agent comprises 1-5 parts of sodium chloride, 100-200 parts of wheat extract and 2-10 parts of sodium caseinate according to weight fractions. The method for preventing freezing injury can increase the proline content in the cells of the sugarcane, reduce the cell membrane permeability, increase the osmotic pressure in the cells, prevent protoplasm from dehydrating and coagulating, and also increase the CAT, SOD and POD enzyme activities, so that the oxidation damage of oxygen negative ions to the cells and tissues is prevented, and the purpose of relieving the freezing injury of leaf cells is achieved.

The present disclosure provides a carrier peptide fragment with the cell membrane permeability that can transfer a target foreign substance from outside a eukaryotic cell into at least a cytoplasm of the cell. The carrier peptide fragment disclosed herein includes an amino acid sequence: RKKRNSKRLTRKK.

The invention discloses a micromolecular fluorescent probe for specific imaging autophagy and a preparation method and application thereof, the fluorescent probe comprises a fluorophore, a linking group and an autophagy targeting group, the fluorophore is an intelligent fluorescence reporter group, the linking group adopts cysteine as a linking unit, and the autophagy targeting group targets LC3 protein; the probe can be used for specifically imaging an autophagy process of a biological sample and screening an autophagy regulator. The micromolecular fluorescent probe has the advantages of being good in cell membrane permeability, simple and convenient in dyeing process and easy to be compatible with other fluorescence labeling technologies, can perform specific recognition and selective imaging on an autophagy related structure, improves the targeting property and imaging credibility of the probe in a complex cell environment, and can be used for detecting autophagy related structures. The method can be used for visual tracing and detection of autophagy flow in living cells under physiological and pathological conditions, and is beneficial for promoting a small-molecule fluorescent probe to analyze an autophagy regulation mechanism and biological functions in real time.