49 results about "Cell membrane permeability" patented technology

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

The present disclosure provides a carrier peptide fragment with excellent cell membrane permeability. The carrier peptide fragment disclosed herein includes the following amino acid sequence set forth in SEQ ID No.: 1: KKRTLRKSNRKKR, and a lysine residue at a 12th position from an N-terminus of the amino acid sequence is a D-form, and the other amino acid residues are L-forms.

The present invention provides a cyclic peptide compound optionally linked to a nucleic acid, the cyclic peptide compound having a cyclic moiety wherein: (1) the cyclic moiety is composed of 13 or 14 amino acid residues; (2) among the amino acid residues constituting the cyclic moiety, the number of N-substituted amino acid residues is 7 or more, the number of amino acid residues in a single side chain is 3 or fewer, and the number of native amino acid residues is 4 or fewer; and (3) the ClogP / total amide bond (ClogP / total AB) is 1.0 to 1.8.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Z34B3 and application thereof. The amino acid sequence of the antibacterial peptide Z34B3 provided by the invention is shown as SEQ ID NO: 1, the secondary structure is an alpha helix, the molecular weight is small, and the antibacterial peptide Z34B3 can change the cell membrane permeability of various gram-negative bacteria including pasteurella multocida, escherichia coli and acinetobacter baumannii, inhibit the generation of cell membranes and play antibacterial and bactericidal roles. Moreover, the antibacterial peptide Z34B3 provided by the invention has low hemolytic activity and no cytotoxicity, and can be applied to preparation of products with bacteriostatic and bactericidal effects and preparation of drugs for preventing and / or treating bacterial infectious diseases. Furthermore, the C tail end of the antibacterial peptide Z34B3 is subjected to amidation modification, and compared with non-modification treatment, the stability can be improved.

The application discloses a kind of strong electron donor triphenylamine modified coumarin dyes, its synthesis method and application;The dye has blue-green light excitation characteristics, the maximum absorption wavelength is 415-425 nm, the maximum emission wavelength is 495-505 nm, and excellent lysosome targeting and positioning ability is shown.It overcomes the problem of low efficiency of traditional coumarin as photosensitizer in molecular structure design, realizes the efficient I type photodynamic effect, can generate active oxygen under light efficiently.In addition, this dye has good cell membrane permeability and low dark toxicity, suitable for biomedical imaging, photodynamic therapy of tumor and other fields.The synthesis method is simple, raw materials are easy to obtain, and the yield is high, suitable for large-scale production.Experiments show that the dye can effectively enrich in cell lysosomes, realize precise killing of cancer cells, and show great potential in cancer diagnosis and treatment integration.

This invention discloses a method and apparatus for preserving fresh Chinese medicinal herbs using a combined electric and magnetic field, belonging to the field of post-harvest preservation technology. The method includes: placing cleaned and disinfected fresh Chinese medicinal herbs in a storage device, while simultaneously applying a low-frequency polarized electric field and a static magnetic field; wherein the output voltage of the low-frequency polarized electric field is 3000 V, the current is 1-5 mA, the frequency is 50-60 Hz, and the electric field strength is 25-35 kV / m; the strength of the static magnetic field is 350-450 Gs. This invention effectively regulates cell membrane permeability and free radical metabolism through the spatial synergistic effect of the low-frequency polarized electric field and the static magnetic field, significantly delaying post-harvest decay of Chinese medicinal herbs, inhibiting microbial growth, maintaining the stability of active ingredients, and achieving good preservation effects even at relatively high storage temperatures. It has advantages such as energy saving, being green, easy to operate, and highly applicable, and is suitable for the storage and preservation of various fresh Chinese medicinal herbs.

A photoredox molecular swimmer, structurally comprising a photoredox center, a redox shuttle, and a flexible PEG chain covalently linking the photoredox center and the redox shuttle. The photoredox molecular swimmer of the present invention enhances cell membrane permeability, promotes entry of extracellular chemicals into living cells, and induces on-demand apoptosis.

The application discloses a curcuminoid xanthene compound shown in formula I, a preparation method of the curcuminoid xanthene compound and application of the curcuminoid xanthene compound as a photodiagnosis and treatment agent. The compound has the characteristics of long absorption and emission wavelength, good stability, high phototoxicity, large Stokes shift, good biocompatibility and good cell membrane permeability. Compared with curcumin, the photosensitizer has a longer absorption wavelength, lower dark toxicity and can generate ROS under type-I and type-II synergistic action, and is expected to be applied to PDT in an anaerobic tumor microenvironment.

The present invention provides a cyclic peptide compound optionally linked to a nucleic acid, wherein the cyclic peptide compound contains a cyclic portion, (1) the cyclic portion is composed of 13 or 14 amino acid residues, (2) among the amino acid residues composing the cyclic portion, the number of N-substituted amino acid residues is 7 or more, the number of amino acid residues having the same side chain is 3 or less, and the number of natural amino acid residues is 4 or less, and (3) ClogP / total amide bond (ClogP / total AB) is 1.0 to 1.8.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to medicinal ink for accelerating lung tumor cell apoptosis and a preparation method and application thereof. Different raw materials are subjected to esterification modification, fermentation or enzymolysis, so that the fat solubility and cell membrane permeability of the composition are promoted, active ingredients can penetrate through a qi and blood barrier more easily to enter lung tissues, the action time is prolonged, the targeting property of the composition is improved, and the conversion of chemical components of traditional Chinese medicines is promoted; a new substance with higher anti-cancer activity is generated or obtained; the bioavailability of each raw material is improved, and a specific enzymolysis product is generated, so that the apoptosis of tumor cells is accelerated. Therefore, different raw materials are specifically prepared, release of active functions of the raw materials is guaranteed from multiple dimensions, and lung tumor cell apoptosis is remarkably accelerated.

The invention discloses a micromolecular fluorescent probe for specific imaging autophagy and a preparation method and application thereof, the fluorescent probe comprises a fluorophore, a linking group and an autophagy targeting group, the fluorophore is an intelligent fluorescence reporter group, the linking group adopts cysteine as a linking unit, and the autophagy targeting group targets LC3 protein; the probe can be used for specifically imaging an autophagy process of a biological sample and screening an autophagy regulator. The micromolecular fluorescent probe has the advantages of being good in cell membrane permeability, simple and convenient in dyeing process and easy to be compatible with other fluorescence labeling technologies, can perform specific recognition and selective imaging on an autophagy related structure, improves the targeting property and imaging credibility of the probe in a complex cell environment, and can be used for detecting autophagy related structures. The method can be used for visual tracing and detection of autophagy flow in living cells under physiological and pathological conditions, and is beneficial for promoting a small-molecule fluorescent probe to analyze an autophagy regulation mechanism and biological functions in real time.

The invention discloses a conductivity-paper roll method-based method for rapidly identifying the vigor of plum-leaf crab seeds, and relates to the technical field of plant cultivation, and the method comprises the following steps: carrying out seed sampling, cleaning and moisture content balance treatment; measuring the conductivity of the seed leachate and judging the integrity of a cell membrane; culturing germination by adopting a wet paper roll method, and calculating a comprehensive vitality index; and comprehensively judging a batch release result according to a threshold value and a rechecking mechanism. According to the method, the cell membrane permeability is represented through the conductivity, the germination potential is reflected through the germination rate, a quantitative threshold value is established by combining double indexes, rapid grading of the seed vigor is achieved, compared with a traditional germination method, the detection period is shortened from 28 days to 14 days or less, operation is easy and convenient, chemical dyes are not needed, the result is traceable, repeatability is high, and the method is suitable for large-scale popularization and application. The method can be widely applied to activity detection, batch release and quality control of the plum-leaf crab and other fruit tree seeds.

A microwave acarus killing instrument comprises a machine body, a microwave emitting device used for emitting microwaves to a cleaning face is arranged on the machine body, the emitting end of the microwave emitting device is located at the bottom of the machine body, a control device used for controlling the microwave emitting device to work is arranged on the machine body, and the control device is electrically connected with the microwave emitting device. The microwave emitting device used for emitting microwaves is arranged on the machine body, mites are removed through the microwaves, due to the dual effects of the heat effect and the non-heat effect generated by the microwaves, the two effects are synergistic, mites and eggs of the mites can be rapidly and thoroughly removed, and no chemical residues exist; the heat effect of microwaves is as follows: the microwaves enable polar molecules such as water molecules in acarid bodies to vibrate and rub at a high speed, high temperature is generated instantly, proteins of the acarid are denatured, biological activity is lost, and the acarid dies; according to the non-thermal effect of microwaves, a high-frequency electromagnetic field directly acts on cell membrane potentials and genetic materials of mites, normal physiological activities of the mites are interfered, cell membrane permeability is changed, and nutrient imbalance is caused.

The invention provides a cyclic peptide permeability prediction method and system based on multi-modal contrast learning, and relates to the technical field of biological information and computer-aided drug design, and the method comprises the steps: obtaining one-dimensional sequence information, two-dimensional topological structure information and three-dimensional space conformation information of a to-be-predicted cyclic peptide; parallel feature coding is carried out on the three modes, and sequence-level features, two-dimensional topological features and three-dimensional geometrical feature embedding are generated respectively; optimizing the sequence-level feature embedding and the two-dimensional topological feature embedding by adopting a double contrast learning strategy to obtain optimized sequence-level feature embedding and optimized two-dimensional topological feature embedding; and performing feature fusion on the optimized sequence-level feature embedding, the optimized two-dimensional topological feature embedding and the three-dimensional geometrical feature embedding to obtain a multi-modal feature vector, and inputting the multi-modal feature vector into a multi-layer perceptron classifier to obtain a binary classification prediction result of whether the cyclopeptide has the cell membrane permeability or not. According to the method, the accuracy, robustness and interpretability of predicting the permeability of the cyclopeptide are remarkably improved.

This invention provides a coumarin-chalcone hybrid compound, its preparation method, and its applications. The structure of the coumarin-chalcone hybrid compound is shown in Formula I. The coumarin-chalcone hybrid compound provided by this invention has a simple and easy synthesis process, readily available raw materials, and can significantly activate the transcriptional activity of Nrf2 at both the cellular and animal levels. It exhibits high safety, good cell membrane permeability, and can be taken orally. After oral administration, it primarily results in high exposure in the liver and can effectively alleviate liver damage caused by acetaminophen and alcohol.

The invention discloses a method for promoting recombinant corynebacterium glutamicum to secrete chondroitin oligosaccharide by changing cell membrane permeability, and belongs to the field of fermentation engineering. According to the invention, a high-copy double-plasmid system is used in corynebacterium glutamicum for coupling synthesis and cutting of chondroitin through a genetic engineering means, so that the yield of chondroitin oligosaccharide synthesized by corynebacterium glutamicum is increased. In order to promote the secretion of chondroitin oligosaccharide, the permeability of cell membranes is increased by quantitatively and regularly adding a surfactant tween-40 in the fermentation process. Through high-copy plasmid expression, culture medium optimization, surfactant treatment and other strategies, the yield of chondroitin oligosaccharide generated by fermentation of the constructed recombinant corynebacterium glutamicum is increased by 37.5%.

The invention discloses peptidoglycan hydrolase as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria, including bacteriostasis and sterilization. The invention discloses application of peptidoglycan hydrolase, and particularly discloses application of peptidoglycan hydrolase as well as pharmaceutical salt or derivative thereof. The application is any one of the following items: A1) application in preparation of bacterial lysis products; a2) is applied to preparation of peptidoglycan products from lysis bacteria; a3) application in preparation of a product for inhibiting bacterial growth; a4) in the preparation of a product for inhibiting the formation of a bacterial biofilm; a5) application in preparation of a product for enhancing the sensitivity of bacteria to antibiotics; and A6) application in preparation of a sterilization product combined with a cell membrane permeation accelerant. A7) application in preparation of a product combined with a cell membrane permeability accelerant to disintegrate a biofilm; and A8) application in preparation of sterilization products. The protein is any one of the following items: B1) a protein with an amino acid sequence being a sequence 2 or a sequence 4; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the peptidoglycan hydrolase in B1), has 80% or more of identity with the protein shown in B1) and has the same activity with the protein shown in B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The protein disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

The invention discloses a ratio type near-infrared fluorescent probe Dn-NIR-Sec of selenocysteine as well as a preparation method and application of the ratio type near-infrared fluorescent probe Dn-NIR-Sec. The chemical structural formula of the ratio type near-infrared fluorescent probe Dn-NIR-Sec is shown as a formula (I). The preparation method comprises the following steps: condensing a compound 1 shown as a structural formula (1) with cyclohexanone under an acidic condition to obtain a compound 2 shown as a structural formula (2); the preparation method comprises the following steps: firstly, preparing a compound 2, then condensing the compound 2 and a compound 3 with a structural formula as shown in a formula (3) to obtain a ratio-type near-infrared dye platform Dn-NIR-OH, and finally reacting with 2, 4-dinitrobenzenesulfonyl chloride to obtain the ratio-type near-infrared dye platform Dn-NIR-OH. Experimental results show that the ratio-type near-infrared fluorescent probe Dn-NIR-Sec can complete rapid fluorescent response to Sec within 5 minutes, real-time dynamic detection is facilitated, selective recognition performance on selenocysteine is remarkable, light stability is good, and meanwhile the ratio-type near-infrared fluorescent probe Dn-NIR-Sec has good cell membrane permeability and low cytotoxicity. Therefore, the probe can be widely applied to the fields of biological detection and the like as a fluorescence detection tool.

This invention discloses a synergistic anti-caries oral care composition and its application. The composition comprises: (a) a single-domain antibody targeting the Streptococcus mutans surface protein PAc; (b) a plant extract complex containing kudzu root extract and phellodendron bark extract; and (c) gallic acid liposomes. This invention also provides a toothpaste containing this composition and a method for its preparation. Experiments have shown that the single-domain antibody can specifically bind to and disturb the Streptococcus mutans biofilm, increasing cell membrane permeability and synergistically promoting the entry of plant extracts into the cell to exert a bactericidal effect, achieving a clearance rate of over 98% for mature biofilms. Through formulation and process optimization, this invention solves the stability problem of antibodies in toothpaste; the product retains more than 90% of its activity after two years of storage at 25°C. The composition and product provided by this invention have the advantages of highly efficient removal of mature biofilms, synergistic effect, safety with low damage, and excellent stability, and have broad application prospects in the field of oral care.

The invention discloses a method for increasing the yield of yeast terpenoids through trace isopentenol synergistic enhanced two-phase fermentation, and belongs to the fields of industrial microbial fermentation, synthetic biology and metabolic engineering. Through the synergistic effect, the total yield of the terpenoids has a qualitative leap compared with that of a traditional single two-phase fermentation system. According to the method, the cell membrane permeability can be actively increased by utilizing the isopentenol at low concentration for the first time. According to the method, only one step of simple operation is added on the basis of an existing mature two-phase fermentation process, complex gene modification or equipment investment is not needed, and the industrial application prospect is wide. The method can effectively relieve product feedback inhibition, significantly improve the yield and simplify downstream extraction, and is mainly used for microbial fermentation production of terpenoids. The invention creatively expands the isopentenol from a pure metabolic substrate to a permeable membrane regulating agent in a specific scene, and opens up a new field for the application of the substance.

The application discloses application of Angelica acutiloba Nakai lactone A in preparation of a product for resisting myocardial ischemic injury, and the myocardial ischemic injury is an increase in cell membrane permeability of myocardial cells or apoptosis, necrosis and fragmentation of the myocardial cells, and the product is a drug or a pharmaceutical preparation. In the application, the Angelica acutiloba Nakai lactone A has low toxicity to normal cells, is derived from traditional Chinese medicines of Angelica and Chuanxiong, is safe, has low preparation cost, and can be used as a promising drug for protecting myocardial cells from injury caused by ischemia.

The invention relates to an effective gravity and electric field control device for realizing zero-gravity, microgravity, low-gravity or aggravated gravity states in a module containing cells. In addition, the invention relates to an effective gravity and electric field control device combining a gravity change mode and an electric field application mode. An effective gravity and electric field control device according to an embodiment of the present invention comprises: an external module having a motion space for an internal module therein and controlling at least one physical quantity related to motion; and an internal module that independently moves with respect to the external module within the movement space by an external force transmitted through the external module, in which the internal module includes an internal space in which the target cell and the specific substance to be introduced into the target cell are loaded in a suspended state.

The invention discloses amino-functionalized Nile blue dye as well as a synthesis method and application thereof, and belongs to the field of organic synthesis. According to the method, by optimizing the catalyst type, the reaction temperature and the solvent system, the generation of isomer byproducts is effectively inhibited, and the reaction selectivity is improved. Compared with the prior art, the synthesis process disclosed by the invention has the advantages of simple reaction steps, mild and controllable conditions, high product yield and high purity; meanwhile, large-scale use of strong acid, strong alkali and expensive noble metal catalysts is avoided, and the production cost is remarkably reduced. The obtained amino-functionalized Nile blue dye has long wavelength absorption, excellent cell membrane permeability and wide pH tolerance range (pH 2-11), and can generate superoxide anion free radicals under red light excitation. Based on the characteristics, the compound can be widely applied to the fields of biological fluorescence labeling and functional material preparation.

The invention relates to the technical field of medicine, in particular to a pyrrole quinazoline diamine derivative and a preparation method and application thereof, the preparation method of the pyrrole quinazoline diamine derivative is mild in reaction condition, the reaction process is easy to operate, and used reagents are low in price and easy to obtain. The compound has excellent broad-spectrum antibacterial activity, bactericidal ability and drug resistance, and has good biological safety to cells. Cell membrane permeability experiments and transmission electron microscopes show that the compound can destroy bacterial cell membranes, so that intracellular substances leak to cause bacterial death; a dihydrofolate reductase inhibition experiment shows that the compound has high affinity with e-DHFR and can effectively inhibit enzyme activity. The pyrroloquinazoline diamine derivative compound provided by the invention effectively inhibits the growth of bacteria and resists the generation of drug resistance through the double-target action of bacterial DHFR activity inhibition and bacterial cell membrane destruction.

The invention discloses application of plant lactobacillus LWQ17 in preparation of a biological agent for inhibiting putrefying bacteria of steamed buns, and belongs to the technical field of microorganisms. The plant lactobacillus LWQ17 obtained through screening can effectively inhibit main putrefying bacteria, namely bacillus licheniformis and bacillus amyloliquefaciens, at the high temperature in summer of the steamed buns, and the plant lactobacillus LWQ17 can destroy the integrity of cell membranes of the bacillus and increase the permeability of the cell membranes of the bacillus. According to the method, the pretreatment conditions of the steamed buns prepared through fermentation of the plant lactobacillus LWQ17 are optimized, the steamed buns are stored for 12-24 hours under the pretreatment conditions that the temperature range is 30-50 DEG C and the humidity range is 40%-85%, and the shelf life of the steamed buns is prolonged. The invention provides the method for prolonging the shelf life of the steamed buns in the normal-temperature and / or summer high-temperature environment, the method has important significance on logistics transportation and storage of the steamed buns, a new direction is provided for a biological preparation for inhibiting bacillus of the plant lactobacillus LWQ17, and the application range of the strain is also widened.

The invention provides an external traditional Chinese medicine composition with a bacteriostatic effect and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition comprises 20-40 parts of pepper, 20-40 parts of fructus cnidii, 10-30 parts of ligusticum, 5-15 parts of pogostemon cablin, 20-40 parts of schizonepeta, 10-30 parts of senecio scandens and 20-40 parts of mint. A response surface experiment design is adopted to optimize an extraction process of traditional Chinese medicinal materials and medicine residues, and the content of effective components such as total flavonoids in the extract is increased; the oil-soluble matrix and the water-soluble matrix are selected, so that the water-soluble medicine components and the oil-soluble medicine components can be better mixed, and the stability is high. The traditional Chinese medicine composition disclosed by the invention can inhibit the formation of bacterial cell membranes and destroy the structures of the bacterial cell membranes, and the permeability of the cell membranes is improved, so that macromolecular substances and proteins are leaked; the intracellular ATP content is reduced, and the extracellular alkaline phosphatase is increased, so that the growth of bacteria is finally inhibited or the bacteria are killed. According to the extraction process, effective components of the medicine are extracted to the maximum extent, and efficient utilization of the medicinal materials is guaranteed.

The invention relates to the technical field of drugs for delaying senescence of lens cells, and particularly discloses a pharmaceutical composition for delaying senescence of lens cells and application thereof, the pharmaceutical composition comprises 0.05-0.1 wt% of cycloastragenol ester derivatives, 0.1-5 wt% of a solubilizer, 1.0-10.0 wt% of a cosolvent, 0.5-2.0 wt% of an osmotic pressure regulator, 0.01-0.5 wt% of a buffer salt, 0.001-0.05 wt% of a preservative, and the balance of water for injection; wherein the cycloastragenol ester derivative is cycloastragenol-3-acetate, and the structural formula of the cycloastragenol ester derivative is shown in Cycloastragenol-3-acetate is applied to delaying senescence of lens cells, compared with a parent compound cycloastragenol, the esterified derivative has significantly enhanced fat solubility and cell membrane permeability, and under the same concentration, the ability of activating the telomerase activity of lens epithelial cells is significantly superior to that of cycloastragenol; stronger biological activity potential is shown.