418 results about "Protoplasm" patented technology

A total nutrient liquid fertilizer containing amino acids comprises, by weights, 6-12 parts of N, 1-6 parts of P, 3-10 parts of K, 1-3 parts of Ca, 1-3 parts of Mg, 9-13 parts of Zn, 2-10 parts of Mn, 1-2 parts of B, 0.5-2 parts of Cu, 0.2-0.5 parts of Mo, 3-10 parts of Fe, 10-15 parts of amino acids, 1-10 parts of a plant growth regulator, 2-5 parts of an organic chelating agent and 30-40 parts of water. The liquid fertilizer provides comprehensive nutrition for plants, enhances the freeze, drought, salt and disease resistance of the plants, enhances the synthesis and translocation of substances in the plants, promotes the formation of adenosine triphosphate (ATP), promotes the photosynthesis and protein synthesis, induces the formation of chlorophyll, promotes the cell protoplasm flow, improves the cell vitality and accelerates the growth of the plants.

The invention discloses cold process soap added with fig protoplasm, and the cold process soap is prepared by taking fresh fig protoplasm, oil, sodium hydroxide and distilled water as principal raw materials, adding essential oil as auxiliary raw materials according to requirements, and preparing at low temperature. According to the invention, protoplasm of squashy fresh figs is added into the formula, wherein the protoplasm is enriched in substances having the effects of repairing, anti-oxidizing and the like on human cells, such as polysaccharide substance, superoxide dismutase and anthocyanin, and nutritional components, such as vitamin and amino acid; the cold process soap disclosed by the invention is prepared at normal temperature, so that effective components and structures thereof of the substances cannot be destroyed; useful effects on human bodies of the cold process soap can be played better; the cold process soap disclosed by the invention does not contain chemical additive products harmful on the human bodies and is purely natural; the cold process soap not only has the effects of daily cleaning, caring skin and hairdressing but also has the health-care effect, and conforms to high-quality living requirements of modern people; and furthermore, the preparation method is simple and safe.

According to the invention, the culture time of paddy rice is shortened by adjusting the growing conditions of paddy rice seedlings; a paddy rice green protoplast is prepared by an enzyme method; the preparation method is optimized, so vacuum pumping and the usage of a highly toxic reagent are avoided; and thus the preparation method of the paddy rice green protoplast is simpler and safer. The protoplast prepared by the method of the invention has a high cell survival rate, and a high transformation rate, is applicable to various analysis such as subcellular localization of target gene codingprotein, protein interaction, western blotting and the like, and is especially applicable to instantaneous expression research of light/chloroplast related gene.

The invention discloses a non-formaldehyde discharging plasmogen and a preparing method and application of the plasmogen. Modified plant seeds adhesive paste by quality portion and thiourea dioxide are put into a mixer with a proportion of 1-3:1, 10-40 water is added and stirred for 1-2 hours; then the non-formaldehyde discharging plasmogen can be made. Then the non-formaldehyde discharging plasmogen is made into discharge printing white discharge pulp used for discharging white printing or into discharge printing colored discharge pulp used for discharging colored printing. The invention solves the problems of free formaldehyde releasing, bad stability, large consumption and high cost caused by the existing discharge agent, provides the non-formaldehyde discharging plasmogen and the preparing method and application of the plasmogen, assures a high white discharge degree when discharging white printing and assures bright color when discharging colored printing and outstandingly displays the unique style and character of the fine pattern of discharge printing.

The invention provides a biological sporoderm-breaking technology and an effective antioxidation method for ganoderma lucidum spore powder. Firstly, full mature ganoderma lucidum spores are screened and put into water to be cleaned, then the ganoderma lucidum spores are heated after being frozen, spore enzymolysis and sporoderm breaking are carried out with one of expansin, cellulose, lysozyme, helicase and the like or the mixture of a plurality of enzymes for 3-24 hours at the temperature of 37-45 DEG C, pH (Potential Of Hydrogen) is regulated to 5.5-6.8 by citric acid, and then a vitamin C is used as an antioxidant of the ganoderma lucidum spore powder. Finally, 1% of sweetener-stevioside is used for removing bitter, and treatment with an instantaneous ultra-high temperature sterilization machine and bagging are carried out. The method disclosed by the invention has a good sterilization effect and high sporoderm breaking efficiency, is low in cost, does not break protoplasm of the ganoderma lucidum spore powder and keeps the original characteristics of the ganoderma lucidum powder.

The invention discloses application of a gene FoPDCD5 (Program Cell Death Protein 5) to regulation of pathogenicity of fusarium oxysporum, and belongs to the field of plant genetic engineering. According to the application of the gene FoPDCD5 to regulation of the pathogenicity of the fusarium oxysporum, the gene is knocked out from the fusarium oxysporum by a homologous recombination method, and aknockout mutant [delta]Fopdcd5 is obtained; by constructing a gene complement vector, the gene complement vector is introduced into a [delta]Fopdcd5 protoplast; and by using a method of random insertion, the gene is completed into the knockout mutant, a complement mutant [delta]Fopdcd5-com is obtained. A pathogenicity test shows that pathogenicity of the knockout mutant [delta]Fopdcd5 is significantly reduced; and pathogenicity of the complement mutant [delta]Fopdcd5-com is restored to a wild-type level. The application of the gene FoPDCD5 to regulation of the pathogenicity of the fusarium oxysporum proves that the FoPDCD5 is necessary for production of conidia of the fusarium oxysporum and response to oxidative stress and pathogenicity. The application is helpful to elucidate a pathopoiesia molecular mechanism of the fusarium oxysporum thoroughly, and target genes are provided for development of effective fungicides.

The invention relates to a novel fruit expander for pears and a use method thereof, and the novel fruit expander can be used for overcoming the defects of high labour of traditionally applying the expander and multiple side effects. The novel fruit expander is formed by scientifically proportioning compound sodium nitrophenolate, gibberellin, pimacol, additives, auxiliaries and a plurality of nutrient elements. The action mechanisms of the novel fruit expander comprise promoting new roots, and increasing absorption area and leaves to increase photosynthetic efficiency, promoting the general circulation of the nutrient substances of tree bodies, and the microcirculation of nutrient substances among cells and in cells, exciting cell activity, enhancing cell division and expansion, improving cell wall permeability, promoting protoplasmic streaming, urging photosynthetic products to be accumulated in fruits, and ensuring robust tree bodies, high yield, early harvest, large fruit size and high sugar content of pear trees. Moreover, the novel fruit expander is free from any side effect and low in cost, and can be easily accepted by pear farmers.

The invention discloses a simple, convenient and rapid preparation method of a cellulose nanofiber. The method comprises the steps that surface carboxylation treatment is performed on microcrystalline cellulose by a chemical reagent, so that the surface of the microcrystalline cellulose is covered with a certain amount of carboxylate radical ions; an aqueous suspension of the microcrystalline cellulose after carboxylation is stirred at a high speed; then the cellulose nanofiber with a high length-diameter ratio is obtained. The method is suitable for carboxylation and nanocrystallization processes of cellulose protoplasm prepared from various natural plants, and facilitates large-scale popularization; all reagents used in the method are common reagents and are low in price; a preparation process is simple, convenient and rapid; the obtained cellulose nanofiber has an ultrahigh length-diameter ratio; the problems of complicated preparation process, high cost and the like of the current cellulose nanofiber are solved.

The invention discloses a ganoderma lucidum fermentation protoplasm cosmetic and a preparation method and application thereof. The ganoderma lucidum fermentation protoplasm cosmetic is prepared through the following steps that dry ganoderma lucidum powder, water and ferment bacteria are mixed to obtain an initial system, and ferment cultivation is performed on the initial system to obtain ganoderma lucidum fermentation protoplasm; wine yeasts are adopted as the ferment bacteria. The invention further discloses the application of the ganoderma lucidum fermentation protoplasm cosmetic in preparing a cosmetic with the whitening and/or aging resisting functions, wherein the cosmetic specifically adopts any one of a facial mask, essence and toner. The prepared ganoderma lucidum fermentation protoplasm cosmetic does not contain any chemical ingredients, can be directly used as finished products of the facial mask or the essence or the toner, is more natural than other existing cosmetics in the market and cannot cause any negative effect on the skins; in addition, active ingredients with small molecular weight can be obtained, and therefore the ganoderma lucidum fermentation protoplasm cosmetic can be more easily absorbed by the skins.

The invention provides a method for producing Ganoderma lucidum mycelium protoplasm, in particular to a method for producing Ganoderma lucidum mycelium protoplasm by adopting liquid fermentation. Based on the total weight of a liquid medium, the liquid medium comprises the following components by weight percent: 1.6-2.5 percent of corn flour, 1-2 percent of soya bean meal powder, 1-2.5 percent of glucose, 0.3-2 percent of collagen, 0.1-0.5 percent of yeast extract, 0.1-0.5 percent of monopotassium phosphate, 0.01-0.08 percent of magnesium sulfate, 0.1-0.3 percent of soybean oil, 0.05-0.1 percent of vitamin E and 0.5-1.5 percent of potato flour. The method has the characteristics of large cubage, high yield, short production period, small occupied area, stable quality, no pollution and the like, and can be used as an important mode for producing raw materials, such as medicines, health care products or cosmetics and the like.

The invention relates to a preparation method of catalpa fargesii leaf blade protoplasts. The catalpa fargesii leaf blade protoplasts are obtained by controlling the ratios and the concentrations of cellulase Onozuka RS and Macerozyme R-10, the concentration of mannitol in enzymatic hydrolysate, enzymolysis temperature, enzymolysis time and other factors. Free protoplasts obtained by the method disclosed by the invention are high in quantity and vitality, and can lay a foundation for researching catalpa fargesii protoplast culture, plant regeneration, genetic transformation, somatic hybridization, protoplast fusion breeding and the like.

The invention discloses a separation and purification method for a Salvia Miltiorrhiza protoplast. The method includes the steps of: induction of a callus and subculture, protoplast separation and protoplast purification. The extraction conditions for a Salvia Miltiorrhiza suspension culture cell's protoplast include that: an enzyme solution suitable for enzymolysis of the suspension culture cell is composed of 1.5% of cellulase, 0.3% of pectinase and 0.5% of macerozyme; a suitable mannitol concentration is 0.4M; the extraction time is 12h; centrifugation is carried out at 600r/min for 5min, then collection and purification are conducted so as to obtain the protoplast with a yield of 1.1*10<6>/gFW, FDA detection shows that the vitality of the protoplast is over 95%, and the calcium ion fluorescence probe fluo-3/AM can be successfully loaded into protoplast. The protoplast extracted under the conditions of the method completely satisfies the follow-up series of study taking a single cell as the study object.

The invention relates to a method for the introduction of one or more molecules of interest in a plant cell protoplast by providing plant cell protoplasts, performing a first transfection of the plant cell protoplast with a composition that is capable of altering the regulation of one or more pathways selected from the group consisting of Mismatch Repair System and Non-Homologous End Joining and/or a composition that is capable of introducing DSBs, performing a second transfection of the plant cell protoplast with one or more molecules of interest such as mutagenic oligonucleotides and allowing the cell wall to form.

The present invention provides a method for separating out rice root xylem parenchyma cell protoplasts. The method chooses the part of the extended and the mature sections of a rice Oryza sativa L. seeding root, which is 2cm to 3cm long and does not has lateral roots grown, uses hydrolytic enzyme liquids with different ingredients and finds the optimal separation conditions to successfully separate out the intact stele xylem parenchyma cell protoplasts. At present, the related report is not published yet at home and abroad.

A cross and tissue culture technology for chinaberry includes such steps as fusing the organs, tissue, cells, embryo and protoplasm of chinaberry, margosa and Melia toosendan, artificial inducing and under lighting and temp to realize cross protoplasm fusion, generating the cell wall of cross chinaberry, splitting to become cell factor, secondary culture and induced growth.

A method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties belongs to the technical field of biological husbandry. The method selects different ganodermas to produce strains, the ganoderma cultivation is carried out, the ganoderma strains with high ganoderma sporocarp yield and good product agronomic trait are selected as the high-yield fusion original strains; and the selenium-resistant experiment is utilized to screen the ganoderma strains with strong rich-selenium capability as the rich-selenium fusion original strains. The method determines one parent strain as the inactivation strain through the hyphal growth speed and form, pigmental secretion, protoplast regeneration rate, protoplast inactivation time and the like; then the fusant screening is carried out through the hyphal form and antagonism, so the operation simplicity and practicability are obtained. The method is adopted to fuse one ganoderma fusion strain which has the sporocarp selenium content and yield higher than those of the original strain, so a good economical benefit is created.

The invention relates to the field of comprehensive utilization of tylosin residues and waste water of vitamin B12, and provides a method which is to use the tylosin residues and the waste water of the vitamin B12 as raw materials, separate and extract protoplasm substances in a mycelium to prepare a thallus extract through microbial fermentation treatment, and apply the thallus extract in a biological medium. The thallus extract and the preparation method thereof open a new path for utilization of the tylosin residues and the waste water of the vitamin B12, and provide possibility of realizing clean production which is a goal of sustainable development for the fermentation industry of the tylosin and the vitamin B12. Furthermore, the method solves the problem that the tylosin residues and the waste water of the vitamin B12 pollute environment and are harmful to human health.

The invention discloses a preparing method of protoplast of edible mushrooms and the protoplast, and relates to the technical field of preparation of engineering cells of filamentous fungi. The preparing method of the protoplast of the edible mushrooms comprises the steps of inoculating edible mushroom strains into a container containing a solid culture medium to be subjected to mycelium culture so that bacterial colonies in which hyphal is grown can be obtained; conducting cell wall enzymolysis on the bacterial colonies to obtain a protoplast coarse solution; filtering, centrifugating and re-suspending the protoplast coarse solution to obtain a protoplast solution. According to the preparing method, a large amount of protoplast can be quickly obtained. Compared with a conventional method, the preparing method of the protoplast of the edible mushrooms is greatly improved in the aspect of speeds, simple in step, and more convenient to operate.

The invention discloses a method for efficiently separating and instantaneously converting a protoplast of artemisia annua L., comprising the following steps: 1) selecting leaves of young and tender artemisia annua and removing lower epidermis, to obtain the leaves of the artemisia annua L. with the lower epidermis removed; 2) placing the leaves of the artemisia annua L. with the lower epidermis removed in an enzymatic hydrolysate for enzymatic hydrolysis to obtain a mixed solution of enzymatic hydrolysis; 3) filtering and centrifuging the mixed solution of enzymatic hydrolysis to obtain a protoplast precipitate, and resuspending to obtain a protoplast; and 4) instantaneously converting the protoplast. The method for separating and converting the protoplast is constructed in the artemisia annua L. for the first time, to obtain the protoplast with yield of 2.749 is multiplied by 10<5>/g FW, viability of 96%, and conversion efficiency of green fluorescent protein of 80%, and the obtained protoplast has large yield and high viability. The protoplast of the artemisia annua L. is utilized as a receptor for conversion, and green fluorescent protein GFP, luciferase LUC and sea cucumberluciferase REN can be successfully expressed.

The invention discloses a paint discharge printing technology with a digital printing effect. The technology comprises the following steps: (a) pre-treating the gray cloth; (b) carrying out ground color dyeing by adopting a dischargeable active dye; (c) drawing through a discharge print technology by adopting an 80%-color color separation drawing soft, manufacturing a net with a silk screen with a size of 100 to 200 mesh; (d) adopting discharge printing protoplasm, adding paint into the protoplasm, evenly mixing, then adding discharge printing powder, modulating discharge printing mill base, on the basis of the four color separation principle, further separating four colors into two sets of colors with different shades, namely 8 sets of colors, wherein one more set, namely discharge printing white slurry is also included, carrying out a printing treatment with printing equipment after the slurry mixing process; (e) baking the processed gray cloth for 3 to 5 minutes at the temperature of 150 to 160 DEG C after the printing process; (f) cleaning the cloth with clean water once with a water washing machine, carrying out a centrifugation treatment so as to remove the water, then carrying out a softening and shaping treatment, and finally rolling the finished product for transportation. The paint discharge printing technology is capable of rapidly producing discharge printing crafts with a digital printing effect, and the products produced by the technology are not easy to duplicate and counterfeit in the market.

The invention discloses a method for forming a full pulp by processing bobbin paper waste pulp residues and producing protoplasm by using the formed full pulp. The method is characterized in that heating is carried out before pulping during the treatment of waste pulp residues, so it is in favor of fibrillation of filers in the waste pulp residues during pulping, a waste paper pulp is processed in grading to respectively prepare a core pulp, a surface pulp and a bottom pulp, the above three pulps are mixed to form a full pulp, and the waste paper pulp grading treatment is in favor of the fiber length equalization; and the full pulp obtained after processing the waste pulp residues is mixed with the full pulp prepared from waste paper boards in proportion to produce the bobbin paper protoplasm. The method changes the waste pulp residues into valuables and reduces the production cost of bobbin paper, and the bobbin paper prepared by using the protoplasm has high strength, and large interlayer bonding force and circumferential pressure. The method is suitable for producing the bobbin paper protoplasm.

The invention discloses a purslane fermentation protoplasm cosmetic and a preparation method and application thereof. The preparation method of the purslane fermentation protoplasm cosmetic comprises the following steps that 20-30 g of dry purslane powder, 250-300 ml of water and 5-15 ml of ferment bacteria with the concentration of 105-108 CFU/mL are mixed to obtain an initial system, and ferment cultivation is performed on the initial system to obtain purslane fermentation protoplasm. Lactobacillus, specifically lactobacillus delbrueckii, is adopted as the ferment bacteria. According to the purslane fermentation protoplasm cosmetic and the preparation method and application thereof, a fermentation engineering technology is adopted, and the active ingredients in the purslane are kept to the maximum extent; compared with a traditional water extraction method, organic solvent is prevented from being introduced, and therefore safety and environment protection are achieved; foreign substances such as enzymes do not need to be added, not only the production cost is reduced, but also the production steps are maximumly simplified, therefore, large-scale production and industrial production can be achieved through the fermentation technique, and the stability of the cosmetic quality can be fully guaranteed.

The invention relates to a healthy wine for preventing and treating cardiovascular and cerebrovascular diseases, which is a low-alcohol beverage brewed with main materials, glutinous rice, water, glucoamylase immersion liquid, microzyme and distillery yeast as raw materials, wherein the main materials are composed of tartary buckwheat, pueraria lobata, rehmannia root and eucommia bark, and the glucoamylase immersion liquid is composed of glucoamylase, 50 DEG protoplasm liquor and water. The wine can be prepared by using solid and semisolid state distillation method, purification-distillation-mixing method, and immersion-brewing double extraction method. In the formula of the invention, rehmannia root invigorates blood, pueraria lobata boosts qi, eucommia bark warms and supplements kidney qi, and tartary buckwheat clears viscera and bowels, in addition, rehmannia root, pueraria lobata, eucommia bark, and tartary buckwheat all have the functions of lowering three highs (hypertension, hyperglycemia, and hyperlipidemia) and nourishing body, and can be used in combination with glutinous rice to enrich and nourish the spleen and stomach and thus to unlock and regulate blood vessel of brain, provide nutrient for body, and prevent and treat cardiovascular and cerebrovascular diseases.

The invention discloses an alga lysing/algal toxin degradation double-effect engineering strain Y1 and a construction method thereof, belonging to the field of microbiology for treatment of blue-green algae. Fusion is performed on parent strains with alga lysing and algal toxin degradation functions by utilizing a protoplast fusion technology to construct the double-effect engineering strain with excellent traits of parents, namely Bacillussphaericus with the collection number of CGMCCNO. 7519. The alga lysing and the derived algal toxin pollution problem are synchronously solved. The protoplast fusion rate can achieve 31.97% under optimal conditions, and the double-effect engineering strain has certain guide effects on solving the problems of blue-green alga bloom and the derived MC-LR (microcystin-LR) pollution and constructing the engineering strain integrating the MC-LR degradation function and the alga lysing property into a whole by performing a protoplast fusion technology, thereby providing reference for achieving the effect on treating both the root course and the symptoms for solving the large-area blue-green alga bloom problem.