7 results about "Animal origin" patented technology

This invention belongs to the field of biotechnology and discloses a monoclonal antibody that specifically binds to the recombinant protein of Clostridium perfringens (Cp) β2 toxin, a hybridoma cell line, and its applications. This invention provides a monoclonal antibody that specifically binds to the β2 toxin protein and a hybridoma cell line CPB2-2A4 (accession number CCTCC NO: C2024312) that secretes this monoclonal antibody. The monoclonal antibody reacts only with the Cp β2 toxin protein and does not cross-react with other pathogens infecting cattle and sheep, exhibiting extremely high specificity. The kit utilizes competitive ELISA technology, using a mouse monoclonal antibody that specifically binds to the Cp β2 toxin recombinant protein as a competing antibody, enabling the detection of Cp β2 toxin antibodies in serum from various animal sources, avoiding species limitations of sample sources. Furthermore, the kit exhibits good reproducibility. The kit has relatively relaxed technical requirements during operation and can be widely used in production for applications such as Cp epidemiological surveys and vaccine immunization level monitoring.

Wound care is the third most costly medical challenge globally, following cancer and cardiovascular diseases. With the growing prevalence of various types of wounds, there is a continuous and increasing need for rapid and effective treatments, particularly those based on natural compounds. Beyond the support of the World Health Organization (WHO) for such approaches, the use of natural products (of both plant and animal origin) that are well tolerated by patients and free of significant side effects is now a clear priority. Iran, with its diverse ecological and climatic conditions, is uniquely positioned as a major producer of medicinal plants and various natural products. The primary goal of this invention is to utilize natural ingredients—including sheep tail fat, beeswax, wild pistachio resin, castor oil, coconut oil, olive oil, turmeric, and honey—to develop an ointment formulation designed to promote the healing of various skin wounds, prevent keloid formation, and provide antibacterial and antifungal effects at the site of application. Key features and innovations of this ointment formulation compared to similar domestic and international products include its carefully selected natural ingredients, lack of side effects associated with conventional chemical drugs, the ready availability and local sourcing of most components, and its very low production cost.

The utility model provides a bottleneck pipettor for detecting animal origin traditional Chinese veterinary medicine residue, and relates to the technical field of bottleneck pipettors, the bottleneck pipettor comprises a shell, a first round tube fixedly penetrates through the bottom of the shell, an adjusting mechanism is arranged on the shell, the adjusting mechanism comprises a round rod, a limiting ring, a first cylindrical hole and two rubber rings, and the round rod is fixedly connected with the limiting ring. A first auxiliary block is fixed to the top of the round rod. According to the bottle opening pipettor for detecting the animal-derived traditional Chinese veterinary medicine residues, the adjusting mechanism is arranged, so that the bottle opening pipettor for detecting the animal-derived traditional Chinese veterinary medicine residues can be butted with reagent bottles with the same caliber and different heights for use, and the universality of the bottle opening pipettor for detecting the animal-derived traditional Chinese veterinary medicine residues is improved; the use effect of the bottleneck pipettor for detecting the animal-derived traditional Chinese veterinary medicine residues is improved, and meanwhile, the use efficiency of the bottleneck pipettor for detecting the animal-derived traditional Chinese veterinary medicine residues is also improved.

The present disclosure provides an animal origin-free (for example, serum-free) cell sheet, such as a mesenchymal stem cell sheet, and a preparation method for the animal origin-free cell sheet. The present disclosure further provides use of the animal origin-free (e.g., serum-free) cell sheet, such as the mesenchymal stem cell sheet, in damage repair of damaged tissue.

This invention relates to a method for evaluating the spectra of biological material of animal, plant, or mixture thereof, the method comprising the steps of: a) detecting spectrometers in a network formed by at least one spectrometer and an input / output device; b) requesting the respective status of each spectrometer in the network of step a), and displaying the detected spectrometers and their status on the input / output device, wherein the status reflects whether the spectrometer is available for recording spectra; c) receiving a selection from spectrometers available for recording spectra on the input / output device; d) recording the spectrum of the sample material of animal, plant, or mixture thereof on the spectrometer selected in step c); e) predicting at least one parameter from the spectrum of step d) using at least one calibration function and / or calibration plot suitable for predicting the value of the parameter. The values ​​of the parameters, wherein the at least one parameter is selected from the content of at least one amino acid, crude protein content, ammonia content, content of all amino acids containing ammonia, content of all amino acids not containing ammonia, crude fat content, dry matter content, crude ash content, energy content, content of at least one biogenic amine, content of at least one anti-nutritional factor, content of at least one sugar, starch content, crude fiber content, neutral detergent fiber content, acid detergent fiber content, total phosphorus content, phytic acid phosphorus content, reactive lysine content, total lysine content, ratio of reactive lysine content to total lysine content, protein dispersibility index, protein solubility, trypsin inhibitor activity, urease activity, and processing condition index (PCI); and f) displaying the predicted results of step e) on the input / output device.

The invention provides an anti-human CD8 rabbit recombinant monoclonal antibody and application thereof. The antibody comprises a heavy chain variable region VH and a light chain variable region VL, a rabbit is immunized with a KLH-coupled CD8 polypeptide antigen, spleen lymphocytes are separated after titer detection is qualified, and single B cell sorting is carried out through a biotin-labeled antigen; positive clone is obtained by a rabbit single B cell culture technology, RNA (Ribonucleic Acid) is extracted and is reversely transcribed into cDNA (Complementary Deoxyribonucleic Acid), and light and heavy chain variable region genes are amplified and cloned into an expression vector for recombinant expression; and finally, the rabbit recombinant monoclonal antibody with high affinity is obtained. The antibody has the capacity of specifically recognizing CD8 + cells in human peripheral blood, is high in affinity and low in animal origin, is suitable for immunodetection such as flow cytometry and ELISA (enzyme-linked immunosorbent assay), and can be used in the fields of immune cell typing, immune function evaluation, immunotherapy monitoring and the like. The invention also provides a light and heavy chain variable region amino acid sequence of the antibody, and a basis is provided for intellectual property protection and further development of the antibody.

Present invention provides a method of producing clarified virus pool of virus to obtain a lyophilized / freeze-dried live attenuated virus immunogenic composition / formulation comprising atleast one or more than one antigens / immunogens. Present invention provides a method of producing a clarified virus pool of viruses such as measles, mumps, rubella. It provides the improved large scale affordable / safe manufacturing processes (encompassing cultivation, purification & formulation stages) that utilize minimum animal origin components, provide high virus yield, ensure virus integrity / stability preservation across manufacturing & storage.