The invention discloses an immunological
animal origin detection kit based on lateral
chromatography and a detection method thereof, and relates to the technical field of
animal origin detection.The immunological
animal origin detection kit comprises a marking pad working
mother liquor, latex is taken and sequentially mixed with 19 # and 20 # diluents,
centrifugation, ultrasonic treatment and ice bath treatment are conducted, 1% of EDAC and 1% of NHS solutions are added for activation according to the specific proportion, and the marking pad working
mother liquor is obtained; mixing a marking
raw material, treating with a confining liquid and a reconstitution fluid, fixing the volume, testing an OD value, and storing at 2-8 DEG C; marking pad working liquid: diluting marking pad working mother liquid to 120OD value by using 7 # reconstitution fluid; marking a pad: spraying
metal according to a specific spraying amount, and
drying at 45 + / -2 DEG C for 12-24 hours; a reaction film: pasting the NC film ss12-15 according to set parameters,
scratching the film, diluting BIO
antibody with a specific
diluent, and making C and T lines with a swine-derived
antibody; a sample pad; according to the invention, based on lateral chromatographic
immunology, an immune technology is adopted, the PCR defects are solved, the operation is simple, the consumed time is short, and the meat source is efficiently detected.