Animal origin-free low-protein culture medium suitable for animal cell product production

Abstract

The invention relates to an animal origin-free low-protein culture medium suitable for animal cell product production, comprising 24 basic metabolism nutrients, 11 vitamins, 3 transferrin substitute compounds, 5 lipid compounds, 2 nucleic acid compounds, 4 hormones and growth factors, 3 antioxidants, 1 shear-resistant protective agent, 1 pH indicator, 2 pH buffers, 9 other inorganic salts, soy hydrolysates adopted to substitute animal origin component, and composition of ferrous sulfate, ferric nitrate and EDTA-2Na adopted to substitute transferrin. The culture medium can be made by dissolving the aforementioned components in triply distilled water. The positive effects of the culture medium are as follows: the culture medium contains no animal origin component, the total protein content is lower than 10mg/L, which helps separate and purify products and is suitable for production of recombinant protein medicaments; the culture medium supports normal growth and long-term subculturing of animal cells; the culture medium can be used without adaptation, is easily prepared and is suitable for massive production of animal cell products.

Description

【Technical field】 [0001] The invention relates to the technical field of culture medium research and development of modern biotechnology, in particular, it is an animal-free low-protein culture medium suitable for large-scale cultivation of animal cells and production of recombinant protein pharmaceutical products such as antibodies and fusion proteins. 【technical background】 [0002] Animal cell culture technology is one of the frontier topics in the field of bioengineering today, and has been valued by the biotechnology community in both basic research and production practice. Since 2000, among all the innovative biotechnology drugs approved by the FDA, only 2 were expressed in yeast, only 6 were expressed in Escherichia coli, and 55 were expressed or produced in mammalian cells, accounting for more than 70%. From June 2003 to June 2004, among the top 10 biotechnological drugs with the highest global sales, 8 products expressed in mammalian cells accounted for 8, and the t...

AI Technical Summary

Problems solved by technology

In the existing serum-free medium, some cannot achieve high-density culture process; although some can support cell growth well, it may lead to the reduction or even loss of the ability of cells to express products; in addition, the formula of commercial serum-free medium is often It is a commercial secret, and its components are complex and expensive, which is not conducive to the research of specific cell line culture technology, nor can it be directly used in the research, development and optimization of cell culture process

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