3034results about How to "Easy to separate and purify" patented technology

The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2 / 3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2 / 0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2 / 0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.

The invention provides a method for preparing fluorine-containing sulphonyl (phosphoryl) imine and fluorine-containing sulphonyl (phosphoryl) imine alkali metal salt. The method comprises the following steps: taking one or two or more than two of Lewis acid, perfluoro-alkyl sulfuryl fluoride and perfluoro-alkyl acyl fluoride as a catalyst, catalyzing anhydrous hydrogen fluoride to react with chlorine-containing sulphonyl (phosphoryl) imine in a high-pressure kettle and then obtaining the fluorine-containing sulphonyl (phosphoryl) imine by depressurization distilling; taking Lewis base as a catalyst, catalyzing the anhydrous hydrogen fluoride to react with the chlorine-containing sulphonyl (phosphoryl) imine, then acting with different alkali metal carbonate and obtaining the correspondingfluorine-containing sulphonyl (phosphoryl) imine alkali metal salt. Compared with the prior art, the method has the characteristics of simple operation steps, mild reaction conditions, low cost, easyseparation and purification of the product, high purity and yield and the like and is suitable for industrialized mass production.

The invention relates a kind of preparation method of bio-diesel oil under the condition of solid ac and alk catalyzing. The character is that under the condition of solid ac and alk catalyzing, gain the bio-diesel oil using the craft of exchange reaction of soja oil with carbinol. Afer the reaction, apart catalyser from reaction liquor by centrifugal machine till delimination in quiet placing, the above is rough output, the below is admixture of glycerol and carbinol. Distil the of above to gain bio-diesel oil and to gain the pure glycerin in the same way. The distilled carbinol can be used again and again. The invention has many advantages, for example, its raw material can be rebirth, its craft is easy and only need normal equipment. Even treat the used oil, the whole process is easier compared with the method in solid ac and alk catalyzing, besides, the process will not produce foul water so it is environmental. Every plant of the bio-diesel accords with standards compared with foreign similar competitive grade and the main plants are close to its of 0# (GB252-1994, superior product)diesel of China.

The invention relates to the field of biology, and discloses a serum-free culture medium which essentially comprises an IMDM (Iscove Modified Dulbecco Medium), L-glutamine, sodium bicarbonate, Hepes, recombinant human insulin, recombinant human transferrin, recombinant human albumin, 2-mercaptoethanol, protocatechuic acid, lipid, amino acid, vitamins, trace elements, Pluronic F-68, hydrocortisone, vitamin C, bonding amine or recombinant human fibronectin, progesterone, putrescine, heparin, serotonin, epidermal growth factors (EGFs), b-fibroblast growth factors (FGF), platelet derive growth factor (PDGF)-BB and insulin-like growth factor (IGF)-I. The serum-free culture medium is clear in chemical components, free from animal sources and serum and safe and ideal in cell cultivation and avoids the doped animal components and unstable batches, and the results of the cultured mesenchymal stem cells show that the total cellular score, the cell phenotype and the secretory cell factors are normal, so that the serum-free culture medium has good industrial application prospect.

The invention provides an ionic liquid consisting of the anions of diimine (vikane) and (perfluoroalkglsulfonyl fluorosulfonyl group) imine and the cation of sulfonium salt, ammonium salt or phosphor salt, as well as method for preparing the ionic liquid through diimine (vikane) and (perfluoroalkglsulfonyl fluorosulfonyl group) imine alkali salt. The ionic liquid can be used as an electrolyte material and applied on the fields like secondary lithium ion batteries, super capacitors, etc.

The invention provides a compound shown in the formula I and a preparation method thereof. The invention also provides a use of the compound shown in the formula I in brivaracetam synthesis and a synthesis method of brivaracetam. The synthesis method utilizes cheap and easily available raw materials and can produce high optical purity brivaracetam.

The invention relates to fusion protein used to prevent and cure I type and II type diabetes and its preparation method and application. It supplies the fusion protein which is formed by glucagon analogy peptide GLP-1, GLP-1 mutant and IgG-Fc, its analogy factor lizard salivary gland polypeptide extendin-4 and IgG-Fc, and the application of the above fusion protein and its DNA used in diabetes prevention and therapy. The fusion protein can increase not only GLP-1 effect, but also the affinity and immunological tolerance of ligand, which is excreted with the form of homogeneity dimmer to improve polypeptide drug effect, overcomes the defect of the GLP-1 for short half-life, and simplifies purification process.

The invention provides a novel Lesinurad midbody and provides a synthesis process of Lesinurad, which is economic, efficient, safe, environment-friendly and applicable to large-scale industrial production. The invention further provides a method for preparing the conventional Lesinurad midbody and Lesinurad by using the novel Lesinurad midbody. When synthesized from the novel Lesinurad midbody provided by the invention, the Lesinurad has advantages that the price of necessary raw materials is low, the raw materials are easy to obtain, heavy metal and solvents which are harmful to the environment are not used, the midbody and a product can be easily separated and purified, the operation is easy, application of thiophosgene which is high in toxicity and not easy to operate is avoided, and the total reaction yield is approximate to or higher than that in the prior art.

The invention provides a tissue engineering nerve graft based on a biological printing technology and a preparation method thereof. The tissue engineering nerve graft comprises an outer tube and a tube inside scaffolds, wherein trophic factors and / or cells can cover the inner surface and the outer surface. A high-fidelity printing operation is performed according to the actual nerve shape demand by utilizing the biological printing technology, a polymer solution is printed into the specified nerve graft in a three-dimensional way by using an ink-jet printer by adjusting the sizes and the quantity of the jet nozzles of the ink-jet printer, the distances from the jet nozzles to a bottom layer and the pulse frequency of a supercharger and writing a control program of specific printing, and the specified nerve graft is applied to treatment of peripheral nerve defect and spinal cord injury.

A process for preparing hydrofluoroether features the reaction between one of trifluoroethene, tetrafluoroethene, hexafluoro propene, etc and one of trifluoroethanol, trifluoropropanol, metanol, etc in organic solvent (DMF or DMSO). Its advantages are high output rate and easy purifying and separating.

The invention relates to an animal origin-free low-protein culture medium suitable for animal cell product production, comprising 24 basic metabolism nutrients, 11 vitamins, 3 transferrin substitute compounds, 5 lipid compounds, 2 nucleic acid compounds, 4 hormones and growth factors, 3 antioxidants, 1 shear-resistant protective agent, 1 pH indicator, 2 pH buffers, 9 other inorganic salts, soy hydrolysates adopted to substitute animal origin component, and composition of ferrous sulfate, ferric nitrate and EDTA-2Na adopted to substitute transferrin. The culture medium can be made by dissolving the aforementioned components in triply distilled water. The positive effects of the culture medium are as follows: the culture medium contains no animal origin component, the total protein content is lower than 10mg / L, which helps separate and purify products and is suitable for production of recombinant protein medicaments; the culture medium supports normal growth and long-term subculturing of animal cells; the culture medium can be used without adaptation, is easily prepared and is suitable for massive production of animal cell products.

The invention relates to a method of preparing three-dimensionally ordered macroporous chelate resin which has a hydrophilic-structure framework and can absorb precious metal irons in aqueous solution, belonging to the field of the chelate resin. The method of preparing the three-dimensionally ordered macroporous chelate resin comprises the following steps: (1) preparing 80-1000nm silica colloidal crystal template; (2) preparing three-dimensionally ordered Poly(N-vinylformamide) or poly(n-vinylacetamide) macroporous material; and (3) preparing hydrophilic three-dimensionally ordered macroporous chelate resin. Compared with the traditional macroporous or gel-type polyvinylamine resin, the novel three-dimensionally ordered macroporous chelate resin prepared with the method has the advantages that the regularly-arranged porous channel system has small diffusion resistance, which is good for the metal irons enter the absorption center from all directions. In addition, the novel three-dimensionally ordered macroporous chelate resin is easy to synthesize, has stable performance, good hydrophilic performance and high selectivity, contains many functional groups, can absorb a large number of metal irons and has better development and utilization values in the aspect of avoiding heavy metal pollution and protecting the environmental water.

The invention provides a method for green synthesis of fluorescent chiral carbon dots. The method comprises the steps of 1, ultrasonically dispersing a carbon precursor and amino acid in deionized water to prepare a transparent aqueous solution or latex, wherein the mass ratio of the carbon precursor to the amino acid is 200:1 to 5:1; 2, putting the mixed solution obtained in the step 1 into a microwave heating device for a microwave heating reaction so as to obtain yellow or brown-yellow liquid; 3, dialyzing the carbon quantum dot solution obtained through the reaction in the step 2 by a dialysis bag of which the molecular weight cut-off is 1,000-50,000 to remove unreacted carbon precursor and amino acid, thereby obtaining fluorescent carbon dots, of which the particle size distribution is narrow, without further purification. The method disclosed by the invention adopts a microwave synthetic method to obtain the chiral carbon dots with the relatively high fluorescent quantum yield through one step, the synthetic method is simple, the required equipment is simple, the repeatability is good, and the method is suitable for preparation of chiral carbon dots on a large scale.

The invention discloses a method for preparing a graphene oxide. The graphene oxide is prepared from a graphite raw material in a mode that mechanical action and oxidation reaction are combined. The method comprises the following steps of: mixing an oxidant with the graphite raw material, subsequently preparing the graphene oxide by employing mechanical action, adding the oxidant after the graphite raw material is subjected to mechanical action so as to prepare the graphene oxide, and adding the oxidant after the graphite raw material is subjected to mechanical action so as to carry out oxidation reaction and prepare the graphene oxide. According to the method, strong acids and strong oxidants required in a chemical oxidation method are abandoned, so that the introduction of byproducts and impurities is greatly reduced, the separation and the purification are convenient, the process procedures are reduced, the treatment time of a mechanical milling method is shortened, the energy consumption is reduced, and the yield is greatly increased. With the combination of the two methods, the graphene oxide can be prepared at one step within the shortest time, the yield is greater than 20%, the graphite oxide can be stably suspended under a centrifugal condition of 16,000rpm without ultrasound, and the method has the characteristics of rapidness, simplicity, convenience, environmental-protection and efficiency.

The invention discloses hyper-branched silicone resin containing active functional group and preparation method thereof. The preparation method comprises following steps : based on mol part, adding 100 parts of alkoxy silanes, 100-150 parts of de-ionized water and 0-0.35 part of catalysts to a reaction container when stirring; reflowing for 2-8h at constant temperature of 40-90 DEG C to obtain solution A; stirring, and dissolving 100-150 parts of blocking agents to a mixed solvent consisting of alcohols and heterocyclic compounds with the volume ratio of 1:2-2:1 to obtain solution B, wherein the volume ratio of the blocking agent to the mixed solvent is 4:6-6:4; stirring, and pouring the solution B to the solution A at 60-90 DEG C, then dissolving a crude product obtained through constant-temperature circumfluence and reduced pressure distillation to aromatics or haloforms solvents, and filtering, carrying out reduced pressure distillation and vacuum drying to obtain the hyper-branchedsilicone resin containing active functional group. The invention has favorable storage stability property, excellent solubility property in low or nonpolar solvents and excellent compatibility with the thermosetting resin, such as epoxy resin, and the like.

The invention discloses a method for hydro-thermally synthesizing a MoS<2> nanoflower with a sulfur-containing biological reagent as a sulfur source. The method comprises the following steps of adding molybdate and L-cysteine or glutathione to deionized water separately for forming a uniform solution, wherein the concentration of the molybdate is 0.002-0.03 M, the mole ratio of S to Mo in raw materials is 4 to 1-2 to 1, and adjusting the pH value of the solution to be 1-7 with hydrochloric acid or ammonia water; shifting the compounded solution to a hydro-thermal reaction kettle with the volume being 100 ml for seal, and conducting a hydro-thermal reaction for 18-36 hours at the temperature of 180-220 DEG C; after the solution is cooled naturally, conducting suction filtration, washing and drying, and obtaining the MoS<2> nanoflower with slice layers accumulated and the surface rough. A MoS<2> product synthesized through the method is at the nanoscale in dimension, is good in dispersion property and has wide application on the aspects of electrode materials, solid lubricant and particularly oil product hydrogenation catalysis.

The invention discloses an NAD analogue as well as the synthesis and the application thereof. The structural formula I of the NAD analogue is a di-phosphate ester compound generated by reacting nicotinamide mononucleotide and corresponding alcohol. An R is a C4-C15 saturated or unsaturated alkyl or saturated or unsaturated alkyl A containing C2-C10 of a heteroatom; and (the R details as the formula II). The NAD analogue can promote the growth of the microorganisms such as colibacillus, saccharomyces cerevisiae, and the like; and the NAD analogue can be also taken as the dehydrogenase cofactor used for catalytic oxidation and reduction reaction.

Provided is a preparation process of trisilylamine capable of preparing high-purity trisilylamine more easily at a lower cost. More specifically, provided is a preparation process of trisilylamine, comprising a step of thermally decomposing perhydropolysilazane under an oxygen-free or low oxygen atmosphere.

The invention discloses an Actinoplanessp. strain and its use in preparation of fidaxomicin. The Actinoplanessp. strain is entophytic Actinoplanessp. N12W0304, is preserved in the China general microbiological culture collection center (CGMCC) on December 26, 2012 and has a preservation number of CGMCC No.7043. A fidaxomicin production process comprises the following steps of preparing an Actinoplanessp. N12W0304 seed solution and fermentation broth, carrying out centrifugation of the fermentation broth to obtain mycelia, carrying out extraction, condensation, dissolution, filtration, resin adsorption and drying of the mycelia to obtain a fidaxomicin crystal crude product, dissolving the fidaxomicin crystal crude product, and carrying out liquid chromatographic separation and pressure-reduction drying of the solution to obtain a fidaxomicin refined product. Under the fermentation conditions, the Actinoplanessp. strain has a fidaxomicin fermentation unit which is more than 1400mg / L and is higher than the fidaxomicin fermentation yield reported at present. The fidaxomicin fermentation process provided by the invention is simple, is suitable for industrial production and realizes a total product yield of 60%.

Production of gama-polyglutamic acid by fermentation is carried out by: culturing bacillus subtills zju-7 strain, i.e., CGMCC No. 1250, in medium with carbon and nitrogen sources, glutamic acid, MgSO4, NaCl and K2HPO4 to obtain ferment liquid, and making it to pass organic solvent settlement and dialysis. The process is easy, yields highly, and costs low with quality products.

The invention belongs to the field of biomedical engineering materials and discloses a nitric oxide loaded cationic polymer, a preparation method therefor and an application of the nitric oxide loaded cationic polymer in the field of biomedicine. A cationic polymer which is used for loading nitric oxide and containing a dendritic polyamide element specifically is a polyethyleneimine grafted dendritic polyamide polymer and has a molecular formula shown in the description. The invention further provides the nitric oxide loaded cationic polymer based on the polymer. The nitric oxide loaded cationic polymer disclosed by the invention can be applied to the field of biomedicine as a nitric oxide donor material through loading NO, is used as a biomedical material and particularly is applied to the preparation of antibacterials; and the nitric oxide loaded cationic polymer has the effects of effectively inhibiting the growth and reproduction of bacteria and fungi, has remarkable inhibitory effects on common oral cavity pathogenic bacteria, dermatophyte, wound infection bacteria and the like, has the functions of promoting wound healing, diminishing inflammation and the like and provides a support for the application of the nitric oxide loaded cationic polymer in preparation of the biomedical engineering materials.

The invention discloses a expansion-type charring agent for a flame-retardant polyolefine material and a synthesis method thereof. The synthesis method comprises the following steps: by using cyanuric chloride as an initial raw material, dropwisely adding amine containing benzene ring, phenol or thiophenol and an acid binding agent into an ice bath to obtain a monosubstituted compound; dropwisely adding aliphatic diamine or aliphatic dibasic alcohol and an acid binding agent, heating to 40-60 DEG C to react, thereby obtaining a disubstituted compound; and finally, dropwisely adding the aliphatic diamine or aliphatic dibasic alcohol and the acid binding agent, heating to 80-110 DEG C, refluxing with a condenser, cooling, washing, and drying to obtain the expansion-type charring agent containing benzene ring, triazine ring and diamino or dialkoxy group. The reaction process adopts a one-pot method, and thus, the invention has the advantages of simple technique, short reaction time and environmental protection in the preparation process; the product has the advantages of high thermal stability, favorable charring effect, low water absorptivity and favorable compatibility with alkene polymers; and after being compounded with ammonium polyphosphate (APP), the product is applicable to polyolefin materials, and has favorable flame-retardant effect.

The invention belongs to the technical field of organic and inorganic composite materials, in particular to a preparation method of organic and inorganic composite fibrous materials with supercritical carbon dioxide. The method includes pre-treatment and activation treatment of cellulose, doping of inorganic sol to the cellulose, etc. The invention makes use of the strong permeability of the carbon dioxide under supercritical conditions, can activate the cellulose with cosolvent, or lead nano particles in the inorganic sol to be absorbed and settled on the surface of the cellulose to form an even, continuous and stable coating layer; meanwhile, some sol particles penetrate into the fiber, so as to dope plenty of cellulose. The supercritical carbon dioxide adopted by the invention is safe, reliable, non-toxic, no pollutant and recyclable, and is environment friendly green solvent. The method of the invention is simple and easy to be operated, and the hybrid material has broad application prospect.

The invention opened non-serum mediums which can proper to culture many kinds of the animal cells. The character of it is to use the DMEM / F12 as the base medium, then add the growth factor, hormone, the essential amino acid and the microelement. It has the functions: (1) it can support many cells and clones; (2) it is same as the serum medium in the growth of the cell and the expression of the product; (3) it support the long heritable culture; (4) it is benefit for the isolation of the product because it contains less protein; (5) it is cheap. We can get the high density of the cells and the product concentration using the medium.

The invention discloses a method for preparing binary or ternary fluorine-containing sulfimide alkali metal salts, a method for preparing ionic liquid by the binary or ternary fluorine-containing sulfimide alkali metal salts, and applications of the alkali metal salts and ionic liquid as electrolytes in carbon-based super capacitors, secondary lithium (ion) batteries, and the like. The method for preparing the binary or ternary fluorine-containing sulfimide alkali metal salts provided by the invention is short in operation steps, easy for product separation and purification, and high in product yield and purity; the binary or ternary fluorine-containing sulfimide lithium provided by the invention has good thermal stability and hydrolysis resistance; a nonaqueous electrolytic solution of the binary or ternary fluorine-containing sulfimide lithium has high conductivity and lithium ion transference number, and also exhibits good oxidation resistance and good compatibility with widely-used electrode materials; meanwhile, the ionic liquid containing the binary or ternary fluorine-containing sulfimide anions exhibits the properties of low viscosity and high conductivity, and has a wide electrochemical window.

The invention discloses a long-chain recombination human bone pattern generating protein-2 and preparing method and application, which is characterized by the following: utilizing gene project technique to grow the protein in the expressing system of escherichia coli and bacillus subtilis; adopting human bone sarcoma cell mRNA to do reverse transcription to obtain the cDNA as form; augmenting nucleotide sequence of entire 114 amino acids naturally from carboxyl end; adding a segment of nucleotide in front of the first codon of primer 5' end; increasing a segment of polypeptide at N end corresponding to amino acid sequence; obtaining long-chain rhBMP-2 gene with molecular weight at 30KD and purity over 95%.

The invention discloses a method for preparing steviol by carrying out catalytic hydrolysis on stevioside by beta-glucosidase, belonging to the field of biosynthesis technology of organic compounds. The method takes the beta-glucosidase derived from aspergillus niger as a catalyst, and can be used for preparing the steviol by carrying out catalytic hydrolysis on the stevioside in single stevioside or stevia rebaudiana extract through temperature programming; the method has the advantages of being high in conversion rate and selectivity, simple in separation and the like; and the enzyme activity loss can be reduced by the temperature programming reaction, so that the utilization rate of enzyme can be improved. The invention provides a new method for preparing the steviol, which is efficient and environment-friendly.

The invention provides a method for synthesizing brivaracetam. Raw materials used in the method are available and inexpensive, a preparation process avoids appearance of chiral isomers difficult to separate, purification means such as column chromatography purification and the like adverse to industrial amplification production is avoided, a synthesis process is not involved with expensive and toxic heavy metal and chiral ligands, and a product brivaracetam with high quality and high optical purity can be obtained.