22 results about "Dermatophyte" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a preparation method of lipopolysaccharide-beta-1, 3-glucan binding protein and application of the lipopolysaccharide-beta-1, 3-glucan binding protein in preparation of a broad-spectrum colloidal gold detection product for dermatophytes. The prokaryotic expression system induces the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein to be efficiently expressed in an inclusion body form, the optimized inclusion body purification-renaturation process is combined to realize batch preparation, and the colloidal gold chromatography technology is integrated, so that the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein is obtained. The bottlenecks of the traditional detection method in sensitivity, broad spectrum and field applicability are broken through.

The invention discloses a dermatophyte nucleic acid rapid detection kit, which comprises: a CRISPR / Cas12a reaction system, which comprises a Cas12a protein and a corresponding crRNA; the fluorescent reporter probe is provided with a 5 '-end FAM label and a 3'-end biotin label; an anti-FAM antibody-fluorescent quantum dot conjugate; the quantum dot immunochromatography test strip comprises a sample pad, a nitrocellulose membrane and an absorption pad, and the nitrocellulose membrane is provided with a test line and a control line; the control line is coated with streptavidin, and the test line is coated with goat anti-mouse IgG. The invention further discloses a corresponding detection method. Detection can be completed within 1 h, operation is easy and convenient, detection sensitivity and specificity are high, and the method is suitable for rapid detection and field application of dermatophyte.

The formulation is an antibacterial and antifungal ointment which comprises of oil of Raphanus sativa seeds, white wax and white petroleum jelly. The ointment acts as a potent antibacterial and antifungal agent and is especially effective against dermatophytes. In clinical trials the ointment was found to be effective, well tolerated and free from irritative and allergic reactions.

This invention relates to the field of traditional Chinese medicine technology, and more particularly to a traditional Chinese medicine composition and its application in the preparation of antifungal drugs. The composition provided by this invention comprises Dioscorea hypoglauca, Cnidium monnieri, Bistorta officinalis, Dioscorea nipponica, Murraya paniculata, Dioscorea opposita, Polygonum multiflorum, and Terminalia chebula. This composition has the effect of inhibiting the growth of superficial dermatophytes (such as Trichophyton rubrum and Candida albicans), which helps to reduce the occurrence of superficial dermatophyte infections and has the potential to be developed into a drug for treating superficial dermatophyte infections.

This invention discloses a deep learning-based method for identifying dermatophyte fluorescence. The method comprises obtaining an input image of dermatophyte fluorescence; encoding the input image using a Transformer model; and decoding and outputting the encoded image using a sequence pooling module to obtain a dermatophyte category prediction result. This method can efficiently and quickly identify dermatophyte species.

The invention provides a skin fungus microscopic examination image identification method and system, and the method comprises the steps: determining a high-frequency component and a low-frequency component of a fungus microscopic examination image, and reconstructing the fungus microscopic examination image into a denoised clear image; synchronously extracting optical features of spore edges during optical diffraction and texture features of spore surface textures for multi-channel feature fusion to obtain an enhanced texture feature map during fungal microscopic examination; according to a spatial topological relation of different feature units in the enhanced textural feature map in a preset neighborhood, generating discrimination features of a spore configuration, and further determining a confidence level of a spore target in the different feature units in the enhanced textural feature map through the discrimination features; and performing adaptive threshold segmentation on the enhanced texture feature map based on all confidence levels, and generating a confidence detection report of the fungal spores on the skin of the patient through a segmentation result. By adopting the scheme of the invention, the key identification features of the spores can be completely reserved while the image noise is inhibited.

The invention relates to the technical field of physical analysis, in particular to a rapid detection method and system for dermatophyte. Obtaining longitudinal offset estimation values and transverse offset estimation values of all target molecule types of the upper envelope line and the lower envelope line of the infrared absorption spectrum, and judging a relative content maximum estimation value of the target molecule types after infrared absorption peak section calibration; the method comprises the following steps: acquiring an adaptive decomposition scale of each similar fluctuation region, performing signal decomposition on the similar fluctuation regions, acquiring a contribution ratio mean value of component signals with similar signal expressions, acquiring the actual relative content of a molecular type in combination with a maximum estimated value of the relative content, and judging whether fungal infection exists in a sample or not. According to the invention, signal overlapping and noise pollution caused by the fact that a skin sample is often suffered from interferon of bacteria and skin components are avoided, and the detection accuracy and the sensitivity to low-component-content fungi or early infection are improved.

A method for treating onychomycosis uses a treatment protocol that atraumatically and subungually administers an antifungal agent dissolved in a liquid pharmaceutical composition to a subungual space to so achieve a minimum inhibitory concentration of the antifungal agent with respect to a dermatophyte in the subungual space. The treatment protocol typically includes a series of administrations, typically at least 4 weeks spaced apart, which achieves cure rates well above 70% after no more than 12 months of administration.

The present application belongs to the technical field of detection reagents, and particularly relates to a dermatophyte detection kit based on a recombinant LGBP-FITC fluorescent probe and application thereof. The present application obtains LGBP-Cys protein by performing C-terminal cysteine site-directed mutation on LGBP protein from Procambarus clarkii; then, the LGBP-Cys protein is directionally coupled with maleimide-activated FITC under mild neutral conditions, so that the problem of protein activity damage caused by traditional random coupling is solved; finally, the coupling product is placed in a composite stabilizing solution containing an anti-quenching agent, so that the stability of the probe and the detection signal-to-noise ratio are significantly improved. The kit is simple to operate, and detection can be completed within 15 minutes. The kit has high sensitivity, high specificity and visualization advantages for common clinical dermatophytes, and is suitable for rapid bedside diagnosis.

The present invention discloses a culture medium for dermatophytes, a preparation method and a method for culturing dermatophytes, and belongs to the field of biotechnology. The culture medium for dermatophytes comprises components including starch, peptone, chloramphenicol, agar, a soluble magnesium salt, a soluble cesium salt, cycloheximide and gentamicin. The preparation method of the culture medium for dermatophytes comprises: stirring and dissolving starch, peptone, agar, a soluble magnesium salt and a soluble cesium salt according to a concentration ratio, sterilizing the mixture, and then adding cycloheximide, gentamicin and chloramphenicol to obtain the culture medium. The present invention also proposes a culture medium for dermatophytes or a method for culturing dermatophytes using the culture medium for dermatophytes, comprising: inoculating dermatophytes into a culture medium and culturing at 28-30°C for more than 3 days; further, the dermatophytes are preferably Microsporum canis. The culture medium can accelerate the colony growth rate of dermatophytes.

The present application relates to the technical field of pet skin care, and specifically discloses a preparation for preventing or treating pet skin fungal infection and a preparation method thereof, which adopts an aerobic-anaerobic ladder type composite fermentation process, takes coix seed, mugwort leaf and purslane as substrates, inoculates bacillus amyloliquefaciens for aerobic fermentation, utilizes enzyme production of the bacillus amyloliquefaciens to break the wall of the herbal components and enriches lipopeptide substances, then inoculates lactobacillus rhamnosus and propionibacterium freudenreichii for anaerobic fermentation, releases free glycoside bases through mutual metabolism, synthesizes short-chain fatty acids such as propionic acid, and obtains the preparation after centrifugal sterilization. The present application realizes the organic integration of the herbal efficacy and the microecological metabolism, can effectively inhibit pathogenic fungi such as microsporum canis, has multiple effects of anti-inflammatory, antipruritic and repairing pet skin barrier, is safe and mild, and has a stable and controllable process.

The invention belongs to the technical field of biological medicines, and particularly discloses a polypeptide with malassezia resisting activity and application of the polypeptide in improvement of seborrheic dermatitis. The polypeptide provided by the invention comprises an amino acid sequence as shown in SEQ ID NO: 1. The polypeptide has a remarkable inhibiting effect on the key pathogenic bacterium-malassezia causing seborrheic dermatitis, experimental data shows that the polypeptide can remarkably inhibit the growth and constant value of the malassezia at a low concentration, and the effect of the polypeptide can be close to that of a positive control ketoconazole. The polypeptide shows an excellent dermatophyte removing effect in a malassezia induced mouse seborrheic dermatitis model, experimental data proves that the fungal load of the skin tissue of the affected part of a mouse is remarkably reduced after administration, and the polypeptide still can keep high biological activity and stability in a complex skin physiological microenvironment, and has a good application prospect. The malassezia can effectively penetrate through a sebum layer to act on thalli, so that the decomposition effect of the malassezia on sebum is effectively restrained.

The invention provides a dermatophyte fluorescence recognition method based on deep learning, and relates to the technical field of image recognition, and the method comprises the steps: carrying out the multi-modal image collection of a target recognition region of dermatophyte, and obtaining a multi-modal image; analyzing a recognition rate of a second mode in the virtual first mode based on the multi-mode image to obtain an image recognition rate; and constructing a fluorescence recognition system based on the image recognition rate, and carrying out fluorescence recognition. According to the application, the technical problem of low recognition rate of skin fungus fluorescence recognition on rare strains in the prior art can be solved, and multi-modal information is virtually reconstructed through the generative adversarial network under the condition of only using single fluorescence image input and is cooperated with the discriminant fusion network; the technical target of accurate, stable and explainable automatic identification of the dermatophyte is completed, and the technical effects of improving the generalization ability of dyeing differences and rare forms and improving the identification rate of rare strains are achieved.

The invention belongs to the technical field of biological detection, and particularly relates to a skin tinea fungus colloidal gold detection kit based on chicken IgY as well as a preparation method and application of the skin tinea fungus colloidal gold detection kit. According to the present invention, the skin tinea fungus rapid detection kit with characteristics of extremely high sensitivity, extremely strong specificity, excellent stability and low cost is successfully prepared mainly through the organic combination of three technical innovation points such as sulfydryl PEG surface modification, composite sealing and chicken IgY antibody system. The clinical performance is excellent, the sensitivity is 93.3%, the specificity is 96.7%, a high-quality scheme is provided for skin tinea diagnosis, and a new high-performance colloidal gold detection path based on chicken IgY is created.

The invention relates to the field of veterinary drugs, in particular to a pharmaceutical composition for preventing and treating rabbit dermatomycosis as well as a preparation method and application thereof. The pharmaceutical composition is prepared from the following raw materials in percentage by weight: 0.5%-2% of clotrimazole, 1%-5% of 5% gentamicin sulfate powder, 20%-40% of sulfur powder, 50%-75% of talcum powder, 2%-6% of 32.5% neomycin sulfate powder and the balance of water. According to the composition, clotrimazole is introduced as a core antifungal component, a targeting action mechanism of specific inhibition of fungal cytochrome P-450 of clotrimazole is utilized, and a non-specific bactericidal effect of sulfur powder is combined, so that synergistic interaction is realized.

Methods and compositions for treating, preventing and controlling fungal infections, including superficial infections, using compounds of the β-carboline class are disclosed. Pharmaceutical compositions comprising β-carbolines alone, or in combination with therapeutically effective amounts of an antimycotic, are administered to a subject to treat or prevent a superficial fungus infection such as those caused by fungi selected from the Candida or Trichophyton genus, infections such as dermatomycosis, diaper rash (yeast dermatitis), athletes' foot (tinea pedis), jock itch (tinea cruris), ringworm (tinea capitis) and cutaneous candidiasis. Methods of treating, preventing or controlling fungal infections in a plant are also disclosed, wherein a composition comprising an agriculturally acceptable amount of a β-carboline and a fungicide is administered to the plant and / or the locus of the plant, β-carbolines having the following formula are preferred. (Formula I).

The present invention discloses a nucleic acid aptamer and a detection kit for detecting Trichophyton rubrum and Microsporum canis, wherein the nucleic acid aptamer includes a nucleic acid aptamer Seq1-LR, and the nucleotide sequence of the nucleic acid aptamer Seq1-LR is a DNA fragment shown in SEQ ID NO.1. The detection kit is a kit comprising nucleic acid aptamers for detecting Trichophyton rubrum and Microsporum canis. The nucleic acid aptamer and the detection kit of the present invention can sensitively, simply, efficiently, specifically and broadly identify and detect Trichophyton rubrum and Microsporum canis, and the nucleic acid aptamer has the advantages of higher affinity and specificity than protein antibodies, no immunogenicity, chemical synthesis, small molecular weight, stability, easy storage and labeling. The nucleic acid aptamer provides a new idea and method for the detection of skin fungal infections, and is expected to achieve rapid and accurate detection of skin fungal infections in the original site clinically.

The invention discloses a primer group for simultaneously detecting eight pet dermatophytes and application of the primer group. The primer group comprises a forward PCR (Polymerase Chain Reaction) amplification primer, a reverse PCR amplification primer and a control primer sequence and a template of eight pet skin fungi, wherein the eight pet skin fungi comprise microsporum canine, microsporum gypsiformis, malassezia, megasporum, trichophyton mentagrophytes, trichophyton fibraureae, histoplasma capsulatum and globularia; the sequences of the primers are respectively shown as SEQ ID NO.1-SEQ ID NO.19. The primers can detect eight pet dermatomycosis, have very high sensitivity and specificity, have certain development, utilization and popularization values, and can provide technical support for clinical diagnosis of pet dermatomycosis.

The present invention relates to a pharmaceutical preparation based on the use of an active ingredient from the family of phosphonium salts derived from Michael acceptors. These four molecules have dermatophyte inhibitory activity of up to 99.9% inhibition of the fungal population intended for use in the treatment of nail fungus, dermatophytes. The CPS molecule inhibits Trichophyton ruhrum at a concentration of 0.031 mg / mL, and also Microsporum canis at a concentration of 0.062 mg / mL. The other compounds CP1, CP3, and CP4 inhibit Trichophyton ruhrum and Microsporum canis at a concentration of 0.25 mg / mL. Compound CP1 inhibits Candida albicans at a concentration of 0.25 mg / mL. Furthermore, these molecules have no lytic or toxic effect on dermatophytes, particularly Trichophyton rubrum, Microsporum canis, and Candida albicans. These synthetic molecules are used as a remedy and treatment for nail diseases caused by dermatophytes, specifically Trichophyton rubrum, Microsporum canis, and Candida albicans.

The invention discloses a primer, a probe, an implementation method and a detection system for qPCR detection of SQLE drug-resistant mutation of dermatophytes, and the method comprises the following steps: identifying a plurality of key mutation sites related to drug resistance by comparing an obtained dermatophytes SQLE gene sequence with a reference sequence in an NCBI database; designing a specific primer, a TaqMan-MGB fluorescent probe and a wild type closed probe modified by the MGB probe based on the mutation site, and verifying the sensitivity and the specificity through the strain level; the method comprises the following steps: constructing a mixed DNA sample model containing drug-resistant strain DNA, wild type strain DNA and human DNA, optimizing a reaction system of single and multiple qPCR detection, and determining an optimal reaction system of qPCR detection. According to the specific primer and probe for qPCR detection of dermatophyte SQLE drug-resistant mutation with specificity meeting requirements and an optimized reaction system for qPCR detection, whether dermatophyte SQLE drug-resistant mutation exists or not can be quickly verified, the sensitivity and specificity of dermatophyte drug-resistant detection are improved, early precise treatment is facilitated, and prognosis of patients is improved.

The present application relates to a method, system, device and storage medium for detecting skin fungi, and relates to the field of fungus detection. The method includes: receiving a number of skin sample images and recording fungus information; dividing the skin sample images into a number of refined areas, randomly combining the several refined areas into a number of groups of sample areas and recording the combination information; processing the several refined areas to obtain a number of feature point descriptors, and obtaining a number of groups of training data based on the several feature point descriptors and the combination information; training a first neural network based on the several groups of training data and fungus information to obtain a prediction model; training a second neural network based on the several skin sample images, the prediction model and the fungus information to obtain a target detection model; when a skin sample to be detected is received, performing skin fungus detection on the skin sample through the prediction model and the target detection model. The technical effect of the present application is to improve the efficiency of skin fungus detection.

The invention discloses a portable fluorescence detection device for skin fungal infection diagnosis, which comprises a first cylinder and a second cylinder, the inner cavity of the first cylinder is inserted into the inner cavity of the second cylinder; the annular cover is fixedly arranged between the bottom end of the first barrel and the bottom end of the second barrel; the ultraviolet irradiation assembly is arranged on one side of the second barrel; the observation assembly is arranged at the top end of the second barrel; and the fixing mechanism is arranged on the other side of the second barrel. According to the portable fluorescence detection device for skin fungal infection diagnosis, by arranging the ultraviolet irradiation assembly, ultraviolet light can irradiate an annular cover through a first reflective mirror, a second reflective mirror and an annular reflective mirror, and then irradiate the skin of a patient in a conical protective cover through the annular cover; doctors can diagnose skin disease conditions according to the fluorescence state reflected by the ultraviolet rays so as to make rapid diagnosis, and operation is convenient and fast.