71 results about "Procambarus" patented technology

The invention relates to preparation and application of umami peptide from procambarus clarkii, and belongs to the technical field of food biology. A preparation method of umami peptides from procambarus clarkia is characterized by comprising the following steps: firstly, separating and purifying polypeptides in a shrimp meat taste extract, predicting peptide activity through a database, carrying out molecular docking and molecular dynamics simulation method characterization, and finally identifying two potential umami peptides, namely LAEPLTFN and YGGEFPARPDN; the umami peptide is artificially synthesized, the fresh property of the umami peptide is verified through sensory evaluation and an electronic tongue method, and the synergistic effect of the umami peptide and other fresh substances is further evaluated. The umami peptide obtained by the method has a composite freshness increasing effect, enriches a fresh water source umami peptide library, and provides a new umami peptide raw material for development of composite freshness increasing seasonings.

The invention provides a method for analyzing interaction between procambarus clarkia arginine kinase and shark source nano antibody. The method comprises the following steps: performing three-dimensional structure prediction on a VNAR sequence, and screening a high-confidence VNAR model by using a Laplace map; docking an amino acid sequence corresponding to the VNAR model with an arginine kinase sequence to obtain an optimal compound model, and analyzing an interaction site of an arginine kinase epitope region and a nanometer antibody complementary determining region CDR3; performing molecular dynamics simulation on the compound model, calculating conformational change indexes and binding free energy through energy optimization, system balance and extended simulation sampling, and outputting a binding stability sequence of the nano antibody and arginine kinase; the method comprises the following steps: screening out a high-stability nano antibody, pre-incubating a fusion protein of the high-stability nano antibody and immobilized arginine kinase to form a compound, adding serum of an allergic patient for competitive binding, quantifying the binding activity of residual IgE through an immunodetection technology, and verifying the inhibition effect of the shark source nano antibody on the sensitization effect of arginine kinase.

The invention relates to a group of specific amplification primers for mitochondrial genes of procambarus clarkia and application thereof, and belongs to the technical field of molecular biology, and the mitochondrial genes of the procambarus clarkia comprise cytochrome c oxidase I subunit genes and cytochrome b genes. The specific amplification primer of the CO I gene comprises a CO I gene upstream primer and a CO I gene downstream primer, wherein the base sequences of the CO I gene upstream primer and the CO I gene downstream primer are respectively shown as SEQ NO.1 and SEQ NO.2; the specific amplification primer of the Cytb gene comprises a Cytb gene upstream primer and a Cytb gene downstream primer of which the base sequences are respectively as shown in SEQ NO.3 and SEQ NO.4. The invention further discloses a kit for detecting the Cytb gene. According to the amplification method of the mitochondrial gene of the procambarus clarkia, PCR (Polymerase Chain Reaction) amplification is carried out on a DNA (Deoxyribonucleic Acid) template solution of the procambarus clarkia by adopting amplification primers of a mitochondrial COI gene and a Cytb gene of the procambarus clarkia. The procambarus clarkia mitochondrial gene specific amplification primer has high specificity, a single target gene can be stably amplified, the length of the obtained procambarus clarkia mitochondrial COI gene segment reaches about 1000 bp, and the length of the obtained procambarus clarkia mitochondrial Cytb gene segment reaches about 800 bp.

The invention discloses a genetic typing chip for synergistically breeding fast-growing and high-temperature-resistant characters of procambarus clarkii and an application method of the genetic typing chip. Aiming at the technical bottlenecks that the traditional procambarus clarkia breeding period is long, the character selection efficiency is low and multi-character collaborative breeding is difficult to realize, a 50K cGPS liquid-phase breeding chip, namely 'Hailongcore No.1', is constructed by taking procambarus clarkia populations of different geographical populations as genetic variation sources; single nucleotide polymorphism molecular markers obviously associated with fast-growing and high-temperature-resistant characters of the procambarus clarkii are screened through whole genome association analysis, and a breeding model is constructed according to the single nucleotide polymorphism molecular markers and is applied to improvement of economic characters of the procambarus clarkii. According to the method, rapid typing of procambarus clarkii individuals can be achieved, the problems that traditional selective breeding is long in period, poor in accuracy, low in efficiency and free of theoretical basis are solved, an efficient molecular tool is provided for improved variety breeding of procambarus clarkii, the breeding efficiency of stress-resistant high-yield new varieties is remarkably improved, and the method has important industrial application value.

The invention discloses an Elodea nuttallii transplanting and maintaining method for breeding procambarus clarkia in a rice field in the technical field of aquaculture, and relates to six links of rice field transformation and treatment, shallow trench digging and base fertilizer application, Elodea nuttallii transplanting, Elodea nuttallii topdressing, Elodea nuttallii maintaining and water quality regulation and control. The method comprises the following steps: applying a fertilizer A according to the amount of 5-10 kg / mu for topdressing, applying a fertilizer B according to the amount of 4-8 kg / mu in March-April, putting duckweed on the water surface in a high-temperature season in May and in a rooting stage and a flowering stage of elodea nuttallii, controlling the putting area of the duckweed to be 50 + / -5% of the area of a culture water body, and applying a fertilizer C according to the amount of 4-8 kg / mu. The root growth and development of the elodea nuttallii are promoted. By means of the waterweed transplanting and maintaining method for breeding the procambarus clarkii in the rice field, the waterweed can still keep vigorous vitality in the middle ten days of July, and the requirement for the breeding environment of the procambarus clarkii in the high-temperature summer season is met.

The invention relates to a procambarus clarkii gonad identification method based on multi-dimensional feature fusion and enhancement, and belongs to the technical field of image identification processing. The invention relates to a procambarus clarkii gonad identification method based on multi-dimensional feature fusion and reinforcement. The method comprises the following steps: cascading a plurality of convolution blocks by adopting a Backbone part in DETR and performing residual connection extraction to form a feature map of procambarus clarkii; the method is characterized in that the feature map is processed through an SDM module and a CSFN module of a Head part in DETR, then results processed by the two modules are subjected to fusion processing, the feature map after fusion processing is input into DETR-Decoder, a detection frame is generated through a DETR-Decoder anchor frame mechanism, and accurate judgment of a target category is achieved by combining DETR-Decoder classification head output.

The invention discloses a virus infection risk assessment method based on procambarus clarkia TRI M23 analysis, which comprises the following steps: acquiring TRI M23 gene and protein expression data of procambarus clarkia in a culture cycle, performing correlation analysis through an expression sequence and a contrast sequence, assessing infection probability, and judging infection aggregation of each region based on a Moran's I index. The infection migration and the infection risk of each region are predicted, and meanwhile, a monitoring strategy and an isolation scheme are dynamically set. The method solves the problems that a traditional method depends on experience evaluation and risk analysis is high in hysteresis quality, the biological detection technology is combined, the risk prediction accuracy is remarkably improved, and a scientific basis is provided for aquaculture virus prevention and control.

The present application relates to the technical field of data processing, and more particularly to a method and system for monitoring the culture environment of procambarus clarkii water flower seedlings, which collects parameter values of various environmental parameters in a current period of time including the current time to form corresponding parameter value sequences; obtains risk values of the environmental parameters at the current time according to the safety range of the environmental parameters; obtains a health degree sequence of the water flower seedlings in the current period of time, and obtains the health impact of fluctuations in the various environmental parameters on the water flower seedlings according to the data fluctuation in the parameter value sequences of the environmental parameters and the data fluctuation difference between the parameter value sequences of the environmental parameters and the health degree sequence; and according to the health impact of the various environmental parameters, the risk values of the various environmental parameters at the current time are weighted and fused to obtain a risk fusion value, and the culture environment of the water flower seedlings is monitored according to the risk fusion value, thereby improving the accuracy of monitoring the culture environment of the water flower seedlings of procambarus clarkii.

The invention relates to the field of crayfish evaluation, and discloses a method and a system for evaluating the WSSV resistance of procambarus clarkii, and the method comprises the following steps: sampling the procambarus clarkii, extracting target tissues from a sample, and extracting total RNA (Ribonucleic Acid) for accurate quantification of concentration and synthesis of cDNA (Complementary Deoxyribonucleic Acid); then TRIM23 gene quantitative detection is carried out on procambarus clarkii through a probe method, the purpose of calculating the Ct value is achieved, and finally the WSSV resistance of the procambarus clarkii is evaluated in combination with a relative quantitative mathematical model. According to the method, high pathogenicity or virus operation is avoided, the safety of the operation process is improved, the monitoring process is greatly shortened, and the accuracy is improved.

The present application relates to crayfish processing technical field, disclose a kind of crayfish cleaning equipment, including cleaning machine and the conveyer belt being arranged below the cleaning machine, the brush washing subassembly is arranged in the cleaning machine, the brush washing subassembly includes several cleaning roller groups, rotation gear is coaxially arranged at the end of the cleaning roller group, arc-shaped rack is arranged in the cleaning machine, and rotation driving part for driving the rotation of the arc-shaped rack, the rotation gear is engaged with the arc-shaped rack. The present application can realize crayfish automatic cleaning and screening process.

The invention belongs to the technical field of molecular biology and genetic breeding, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker related to the individual meat percentage character of procambarus clarkii and application. The SNP marker disclosed by the invention is located at the 10593223rd basic group of No.73 chromosome of procambarus clarkii, the mutation type is T / C, and the preferable genotype is TT. The SNP is used for verifying in a procambarus clarkia family, and the SNP in the family is Chr73: 10593223 Tgt; the SNP marker has a significant difference in meat percentage characters, which proves that the SNP marker is suitable for procambarus clarkii individuals, and shows that the SNP marker has an application prospect in procambarus clarkii growth character molecular marker assisted breeding.

This utility model relates to the field of aquaculture technology, specifically to a quantitative, staged feed dispenser for red swamp crayfish (Procambarus clarkii). It includes a support frame, a storage bin fixedly installed inside the support frame, a spiral feeding pipe connected to the bottom of the storage bin, a water pump connected to the other end of the spiral feeding pipe, a flexible hose connected to the top of the water pump, a nozzle connected to the other end of the flexible hose, and a rearing trough located below the nozzle. By incorporating a feed dispensing mechanism, this utility model can flexibly adjust the feed particle size and spraying range according to the growth stage of the red swamp crayfish. This not only avoids the digestive problems caused by juvenile crayfish consuming large-particle feed, or the nutritional deficiencies of adult crayfish consuming small-particle feed, but also prevents excessive feed concentration, which can lead to significant waste in high-density aquaculture environments. This achieves the effect of avoiding growth stunts caused by unsuitable particle size while ensuring uniform feed distribution and preventing localized accumulation or food shortages.

The utility model discloses a crayfish cleaning mechanism which comprises a cleaning chamber and an elutriation chamber, a notch used for communication is formed between the cleaning chamber and the elutriation chamber, a gate is arranged at the notch in a sliding mode, a plurality of cleaning rollers are arranged in the cleaning chamber in a rotating mode, the bottom of the cleaning chamber is in a slope shape inclining towards the direction of the notch, and the gate is connected with the cleaning chamber. A first water inlet is formed in one side of the top of the cleaning chamber, a limiting plate is arranged in the washing chamber, a washing cage is arranged on the limiting plate, an opening is formed in the top of the washing cage, and the four walls and the bottom of the washing cage are each of a net-shaped structure. After washing is completed, the gate is opened, so that the crayfishes and sewage after washing slide into the washing chamber, the crayfishes are left in the washing cage, the sewage penetrates through the washing cage and is discharged from the water outlet, then water is injected into the washing chamber through the second water inlet, the crayfishes are rinsed for the second time, and sewage residues on the crayfishes are removed.

The invention provides a method for rapidly predicting the meat content of female procambarus clarkii. The method for rapidly predicting the meat content of the female procambarus clarkii comprises the following steps: S1, placing living female procambarus clarkii on a collection table, and applying broadband sound wave excitation to the female procambarus clarkii by using a micro vibration device to obtain a resonance response signal; s2, performing characteristic measurement on the female procambarus clarkii to obtain morphological parameters, and obtaining a meat content linear prediction model based on the morphological parameters; and S3, carrying out spectrum analysis on the resonance response signal and extracting resonance characteristics. Compared with a traditional method of weighing after slaughtering, the method for rapidly predicting the meat content of the female procambarus clarkia does not need to damage the procambarus clarkia bodies, keeps the integrity of samples, facilitates repeated screening and subsequent observation of genetic breeding, is simple and convenient to operate and short in time consumption, greatly improves the detection efficiency, and is suitable for large-scale high-throughput application.

The invention provides application of protocatechuic acid in regulating intestinal flora of procambarus clarkii and flavor amino acid composition in muscle of the procambarus clarkii, and belongs to the technical field of development of aquatic feed additives. It is found that the protocatechuic acid can reduce the abundance of Vibrionaceae in the intestinal tract level of the procambarus clarkii, improve the abundance of Rhodobacteroceae, Lactobacillus and Bacillus, reduce the abundance of Vibrio in the intestinal tract level of the procambarus clarkii, and improve the abundance of Halocynthiibacter and Ruegeria, so that the composition of flavor amino acids in the muscles of the procambarus clarkii is improved. The invention further provides a procambarus clarkii feed and application thereof in procambarus clarkii culture, the content of flavor amino acids in procambarus clarkii can be effectively increased, the muscle quality of procambarus clarkii is improved, the cost is low, and the culture method is simple.

The invention provides bacillus subtilis with high-valued procambarus clarkii processing by-products and application of the bacillus subtilis in aquaculture. The bacillus subtilis with the high-valued procambarus clarkii processing by-products and the application of the bacillus subtilis in aquaculture comprise the following steps: S1, pretreating the procambarus clarkii processing by-products to obtain fermentation raw materials; s2, inoculating the fermentation raw material with bacillus subtilis; and S3, carrying out fermentation under the conditions that the temperature is 30-40 DEG C and the pH value is 6.0-7.5. According to the bacillus subtilis with the high-valued procambarus clarkii processing by-products and the application of the bacillus subtilis in aquaculture, the procambarus clarkii processing by-products are converted into feed additives or functional products with high additional values through a fermentation process. The immunity and the growth speed of aquatic animals can be improved, and the disease resistance of the aquatic animals can be enhanced, so that the overall benefits of aquaculture are improved.

The utility model discloses underwater feed throwing equipment for procambarus clarkii, which relates to the technical field of feed throwing and comprises a culture pond, a first supporting strip, a second supporting strip and a special-shaped plate. The first supporting strip is arranged above the culture pond; the second supporting strip is arranged on the side of the first supporting strip; the special-shaped plate is connected into the second supporting strip in a sliding mode. A conveying assembly for conveying feed is arranged on the special-shaped plate, and a feeding assembly for feeding the feed is arranged at the top of the special-shaped plate; a driving assembly for driving the special-shaped plate is arranged on the second supporting strip, a moving assembly for moving the second supporting strip is arranged on the first supporting strip, a lifting assembly for lifting the first supporting strip is arranged on the first supporting strip, through the throwing assembly, the effect of throwing fodder underwater is achieved, the fodder is prevented from being directly scattered on the water surface, and the fodder throwing efficiency is improved. The problem that the procambarus clarkii floats away due to the action of wind blowing, flowing water or diffusion and cannot sink into the underwater area where the procambarus clarkii often moves is solved.

A crayfish orientation correction device is characterized by: a funnel-shaped conveyor belt, a rotating wheel, and a connecting rod. The funnel-shaped conveyor belt feeds the crayfish, and the frame-shaped rotating wheel feeds the crayfish. The inclined guide surface cooperates with the frame-shaped rotating wheel to complete the orientation correction and quantitative conveying of the crayfish, solving the problems of complex conveying structure and random crayfish posture in existing automatic aquatic product processing equipment.

The invention discloses a procambarus clarkii in-vitro incubation device and an in-vitro breeding method, and belongs to the technical field of shrimp in-vitro breeding. The device comprises a transparent bucket body, a 90-degree elbow joint, a three-way pipe, an aeration pipe, a water outlet pipe, an air valve, a water outlet valve, a mesh and a filter screen. The in-vitro cultivation method comprises the following steps of: placing an egg-carrying lobster in clean water for temporary cultivation, stripping an embryo from the abdominal limb of the lobster by using a Michi comb after a lobster egg develops to a protointestinal stage, cleaning the embryo, removing residues, transferring the embryo into the device for incubation, pouring a self-made culture solution into the transparent bucket body, opening an air valve, and aerating the bucket through an aeration pipe to suspend the embryo. The procambarus clarkii in-vitro hatching device has the advantages of being unique in design, low in cost, visual, large in capacity, high in efficiency and easy to operate, a large number of shrimp eggs can be contained at the same time, the requirement for large-scale hatching is met, the shrimp eggs obtain appropriate water flow, oxygen and nutrition supply in the hatching process, and therefore the hatching success rate and the fry quality are improved.

The invention discloses a procambarus clarkii breeding pond, and belongs to the technical field of breeding equipment, the procambarus clarkii breeding pond comprises a breeding pond module and a water pumping module, the breeding pond module is used for placing female shrimps carrying eggs, one end of the water pumping module is arranged on the pond bottom side of the breeding pond module, and the water pumping module is used for enabling aerated circulating water to flow back into a breeding pond so as to increase the dissolved oxygen amount of pond bottom water; according to the invention, the pump flow assembly and the flow release assembly for pump flow are arranged between the breeding pool and the backflow pool, so that the circulating water after aeration and oxygenation can flow back into the breeding pool, the circulating water flows into the breeding pool at a low speed, and the breeding pool is more convenient to use. The residual feed is uniformly distributed to the bottom of the pond while circulating water change and uniform increase of dissolved oxygen at the bottom of the pond are realized, and accumulation and decay of the feed are reduced.

The invention discloses an intensive breeding device of procambarus clarkii and an intensive artificial breeding method of the procambarus clarkii, and relates to the technical field of aquaculture. The intensive breeding device comprises a parent shrimp inhabiting and mating nest structure and an egg-carrying shrimp hatching and larva breeding nest structure which are arranged in a parent shrimp breeding pond and a hatching and larva breeding pond respectively. The parent shrimp inhabiting and mating nest structure comprises an inhabiting frame and an inner nest body, the inhabiting frame is a right trapezoid frame body, and multiple layers of partition plates are arranged in the middle of the right trapezoid frame body; the inner nest body is a right trapezoid body, and a plurality of inhabiting nests are formed in the inner nest body; an egg-carrying shrimp hatching and larva breeding nest structure comprises a hatching and breeding frame, hatching nest units and side nets. According to the device, the space utilization rate of the parent shrimps and the larvae in the parent shrimp breeding pond and the hatching and larva breeding pond is effectively increased, and the bearing number and the breeding density of the parent shrimps and the larvae in unit water are greatly increased. The invention further discloses an intensive artificial breeding method, intensive and batch production of egg-carrying shrimps and juvenile shrimps of the procambarus clarkii is achieved, and the productivity can be improved by more than 5 times.

The utility model belongs to the field of agriculture, and particularly relates to a crayfish feed feeding device capable of automatically adjusting the feeding amount, a plurality of buoyancy driving assemblies are arranged outside a bottom plate, a sonar detector is arranged on the lower end face of each buoyancy driving assembly, a material stirring barrel is arranged above the bottom plate, a plurality of stirring scrapers are rotationally arranged in the material stirring barrel, and the stirring scrapers are arranged in the bottom plate. A rotating plate is rotationally arranged on the outer side of the lower end face of each stirring scraping plate, a plurality of discharging holes are formed in the inner bottom face of the material stirring barrel, a second discharging baffle is rotationally arranged on the portion, on the lower end face of the material stirring barrel, in each discharging hole, and a limiting piece is arranged on the portion, on the upper end face of each second discharging baffle, in the material stirring barrel; and a feed storage assembly is arranged above the mixing barrel. Through the arrangement of the sonar detector and other structures, the device can detect the number of the crayfishes in a water area, quantitative feeding is conducted according to the number and density of the crayfishes, and therefore the low growth rate of part of the crayfishes caused by uneven feed feeding is effectively avoided.

The application discloses a SNP molecular marker related to growth performance of Procambarus clarkii, which is located in the second last intron region of an insulin-like growth factor receptor gene of the Procambarus clarkii, and specifically at the 42659th bp of the full-length genome, and the alleles are C and T, wherein the average body weight of an individual with a TT genotype is significantly greater than that of an individual with a CC genotype and an individual with a CT genotype. The application further discloses primers for amplifying the SNP molecular marker and a growth performance typing identification method of the Procambarus clarkii, and has important significance in improving the growth performance and breeding efficiency of offspring shrimps, increasing the breeding yield and income and the like.

This invention discloses an integrated automatic processing equipment for crayfish deheading and deveining, characterized by its relevance to the field of aquatic product processing technology. The equipment includes a frame, a feeding and conveying mechanism, a chain-type clamping and conveying mechanism, an orientation mechanism, a deheading mechanism, a deveining mechanism, and a diversion and collection mechanism. The feeding and conveying mechanism orderly conveys the crayfish to the crayfish clamps; the orientation mechanism uses visual recognition and mechanical correction to maintain a uniform orientation of the crayfish; the deheading mechanism removes the crayfish heads; the deveining mechanism uses a synchronous chain to drive a wire-stripping clamping and pulling assembly to extract the intestines; and the diversion and collection mechanism separately collects the crayfish heads and tails. This invention achieves continuous automated processing of crayfish from orderly feeding, head and tail orientation, deheading, deveining to diversion and collection, solving the problems of low manual efficiency and poor hygiene. It has the advantages of integrated operation, accurate orientation, reliable clamping, low damage, and hygiene friendliness.

This utility model discloses an intelligent monitoring and regulation device for water quality in red swamp crayfish farming, relating to the field of red swamp crayfish farming technology. It includes a base plate, with a farming pond located at one end of the upper surface of the base plate. A top plate is fixedly connected to the upper surface of the farming pond. A waste bin is fixedly connected to the end of the upper surface of the base plate away from the farming pond. A waste discharge and aeration mechanism is installed at the waste bin and the upper surface of the top plate. A water exchange tank is fixedly connected to the end of the upper surface of the base plate away from the waste bin, and a water exchange mechanism is installed at the top of the water exchange tank. A multi-parameter processor collects key water quality parameters such as pH, dissolved oxygen, and temperature in real time. Once the data exceeds a threshold, the central processor automatically triggers the servo motor, sludge suction pump, water exchange pump, and return pump to work collaboratively. This completely eliminates the limitations of manual inspection and adjustment, achieving full automation of water quality monitoring, analysis, and regulation, significantly improving the efficiency of aquaculture environment regulation and effectively reducing labor costs.

The present application relates to lobster product processing technical field, disclose a kind of crayfish processing cooking device, including cooking bin, independent temperature control, matching component, dispersing weighing assembly and control component;The bottom of cooking bin is provided with steam heating part, independent temperature control is located above steam heating part, to different weight specifications crayfish independent heating conveying is carried out.This application has the following advantages and effects: crayfish can be divided into three different specifications by weight, three different heating temperature conveying belts are provided in the cooking bin, different weight specifications crayfish can be sent into corresponding temperature conveying belt respectively, by controlling conveying speed, to realize the purpose of uniform heating for crayfish of different sizes;After crayfish blanking, it can be accurately sent into corresponding conveying belt according to weight by dispersing mechanism, in an automated manner, to ensure processing efficiency.

A method for cave cultivation of procambarus clarkia seedlings, comprising the following steps: rice field selection, rice planting, parent shrimp preparation before stocking, parent shrimp stocking, parent shrimp cultivation, stress cave digging and cave entering, field drying, rice harvesting, rice stubble green-up, cave cultivation, induced cave exiting and cage catching; wherein the rice field selection is to select a rice field with sufficient water source and convenient irrigation and drainage, the relative two sides of the field have height difference, the field ridge is reinforced and has a mouse, frog and snake prevention iron fence, and a polyethylene net is surrounded along the inner side of the field ridge; the parent shrimp stocking is to stock female parent shrimp with ovary maturity of stage III, and the female to male ratio is 1:1; the parent shrimp cultivation comprises feed feeding, water quality control and daily management; the stress cave digging and cave entering is to drain water; the rice harvesting is after the field drying; the rice stubble green-up is to keep shallow water to promote growth after the rice harvesting until new grass seedlings are generated; the cave cultivation is to regularly pour horse race water rich in food; and the induced cave exiting is to induce by water injection and feed feeding. The cave cultivation method can improve the survival rate of the seedlings and the parent shrimp.

The invention provides application of chlorogenic acid and resveratrol in improving the growth performance and / or disease resistance of procambarus clarkii, and belongs to the field of aquatic nutrition. It is found that the chlorogenic acid and the resveratrol are jointly used according to the mass ratio of (1-3): (1-3), the specific growth rate and the survival rate of the procambarus clarkia can be remarkably increased, and the white spot syndrome virus resisting capacity of the procambarus clarkia is improved. The invention further provides a feed additive for improving the growth performance and the disease resistance of the procambarus clarkii and a procambarus clarkii feed, and the health level and the economic benefits of the procambarus clarkii can be comprehensively improved.

The invention provides a procambarus clarkii breeding device and method, the procambarus clarkii breeding device comprises a main rack, a feeding disc, a feeding disc, a distributor mechanism and a presser mechanism, the feeding disc is slidably arranged on the floating main rack, the feeding disc is slidably arranged on the inner side of the feeding disc, and a cavity cylinder is fixedly assembled in the middle of the feeding disc; the feed distributor mechanism and the feed pressing mechanism are arranged in the cavity cylinder, the feed distributor mechanism is used for uniformly releasing feed of the procambarus clarkii into the culture pond, and the feed pressing mechanism is used for continuously filling the feed distributor mechanism with feed to be output. The procambarus clarkii feeding device is simple in structure, can separate procambarus clarkii of different body types in the feeding process by matching with the material distributor mechanism and the material pressing mechanism, reduces fighting in the food grabbing process, provides a safe feeding environment for small-body procambarus clarkii, and reduces energy loss caused by fighting of the procambarus clarkii groups.

The invention discloses an anti-lipopolysaccharide factor of procambarus clarkia and antibacterial application of a core antibacterial structural domain of the anti-lipopolysaccharide factor, and belongs to the technical field of genetic engineering and molecular immunology. According to the technical scheme, the gene sequence of the procambarus clarkia anti-lipopolysaccharide factor is shown as SEQ ID No.1 and SEQ ID No.4 in a sequence table. The invention further specifically discloses an expression method of prokaryotic protein of the procambarus clarkia anti-lipopolysaccharide factor and an identification and synthesis method of a core antibacterial structural domain. The aeromonas hydrophila and the procambarus clarkii can be used for remarkably killing the aeromonas hydrophila in vivo and in vitro, and the survival rate of the procambarus clarkii infected with the aeromonas hydrophila can be remarkably improved. The anti-lipopolysaccharide factor and the core antibacterial structural domain thereof are stable in structure, simple to prepare, definite in component, safe and stable, and can effectively prevent the procambarus clarkii from death caused by bacterial infection.