165 results about "Procambarus" patented technology

The invention put forwards an excised cultivation technology of procambarus clakii eggs according to developmental biology principle. The key points of the technology are the separation between parent prawns and eggs of berried prawns, the classification of the eggs, and respectively artificial hatching of the eggs. The steps of the invention are as follows: firstly, the berried prawns are sterilized; secondly, the parent prawns and the eggs are separated; thirdly, the eggs in different development stages are classified; fourthly, nidation of the eggs; fifthly, the hatching of the eggs. The invention has a high egg hatching rate, uniform sized young hatching prawns and a high commodity value. The cultivation technology can effectively prevent the young prawns from eating each other; therefore the survival rate of the young prawns is improved. Because the berried prawns do not need to be reared, cultivation cost is reduced and the transportation of prawn eggs is more convenient.

The invention discloses an earthen pond breeding method of procambarus clarki. The method comprises the following technical steps: (1) pond reconstructing, cleaning and sterilizing; (2) preparing before parent shrimp breeding; (3) parent shrimp breeding; (4) fodder feeding; (5) daily management; (6) wintering management; (7) seedling cultivation; and (8) seedling catching. After the testing of themethod, a large number of seedlings are produced in a base of about 5,000 mu (1 mu is about 666.7 square meters), more than 50,000 shrimps can be bred per mu, and specifications are 140 to 160 high-quality shrimp seedlings per kilogram, thus offering safeguard for the breeding of grown shrimp. Though the breeding amount of lobster individuals is small, survival rate is relatively high. A large amount of parent shrimps ensures the amount of seedlings. The breeding of procambarus clarki is not synchronous and seedling specifications in a pond are inconsistent, but seedlings required by production can be bred and demand for future breeding can be fulfilled. With batches of seedlings, future production and development can be guaranteed.

The invention provides a procambarus clarkia deveining machine which comprises a shrimp tail conveying mechanism, a shrimp intestine removing mechanism, a main stand and a subordinate stand, wherein the shrimp tail conveying mechanism is fixed on the main stand; the shrimp intestine removing mechanism is fixed on the subordinate stand; the shrimp tail conveying mechanism is used for conveying and clamping shrimp tails; and the shrimp intestine removing mechanism is used for shearing tail fans and pulling out shrimp intestines. The deveining machine disclosed by the invention is skillful in structure, and since the shrimp tail conveying mechanism and the shrimp intestine removing mechanism are matched in use, the deveining machine disclosed by the invention can be used for removing the shrimp intestines without damaging the shrimp tails. The deveining machine is high in production efficiency and mechanical degree, and can be used for completely replacing manual labor to remove the shrimp intestines, saving expensive labor cost and greatly reducing the production cost of the shrimp tails; and the deveining machine disclosed by the invention is suitable for the large-scale processing of the procambarus clarkia tails.

The invention discloses a Chinese herbal medicine immunopotentiator for resisting white spot syndrome virus (WSSV) of procambarus clarkia, as well as a preparation method and an application thereof. The preparation method comprises the following steps: milling rheum officinale, epimedium, astragalus membranaceus and isatis root and fully mixing the ingredients in proportion to prepare the powdery immunopotentiator. The Chinese herbal medicine immunopotentiator disclosed by the invention is stable and wide in raw material resources, simple to prepare, and low in cost, is based on the immunomechanism of shellfish to provide Chinese herbal medicines for the shellfish, and can be used for improving the immunity and oxidation resistance of a body during application, improving the capacity of the procambarus clarkia for resisting WSSV infection, and promoting the growth of the procambarus clarkia.

The present invention discloses a feed for procambarus clarkia. The feed is prepared from the following components in parts by weight: 30-35 parts of corn flour, 25-30 parts of puffed soybean meal, 5-10 parts of distillers' grains, 2-3.5 parts of yeast powder, 20-25 parts of flour, 10-15 parts of spirulina platensis powder, 5-10 parts of green vegetable leaves, 2-3 parts of chicken intestine powder, 5-10 parts of earthworm powder, 2-3 parts of dried small ctenopharyngodon idellus powder, 5-10 parts of sunflower seed oil residues, 0.2-0.4 part of garlicin, 1-3 parts of Chinese yams, 2-3 parts of wild chrysanthemum, 0.5-1.2 parts of licorice, 2-4 parts of leonurus artemisia, 2-3 parts of honeysuckle, 1-1.5 parts of compound vitamins, and 1-1.5 parts of trace elements. Advantages are as follows: the feed contains various nutrients, is scientific in formula and comprehensive in nutrition, and satisfies basic nutrition of the procambarus clarkia. At the same time, the vitamins and trace elements are added, so that the feed ensures the procambarus clarkia to grow rapidly, improves immunity, and is free of toxic and side effects, and also harmful substance residues.

The invention discloses a procambarus-clarkii environment-friendly sinking puffing feed and a preparing method. The feed is prepared from protein source-fat source-sugar source raw materials, composite vitamins, composite mineral substances, antioxidants, phagostimulants and adhesives in a certain proportion. The preparing method includes the steps that A, the feed raw materials are respectively smashed and screened, the obtained mixture and the adhesives are mixed to be even, the fat source raw materials are added while stirring, water is adding while stirring after even mixing, and feed base materials are prepared; B, the feed base materials are input into a double-shaft extrusion puffing machine, a slicing motor is started, and the extrusion puffing machine operates in the modes that a switch is switched on, a main motor is started, the feed base materials are matured and extruded through a mold hole for extrusion puffing, semi-particle feed is obtained and matured, and the puffing particle feed is obtained. The formula is reasonable, use is convenient, the culturing cost and the incidence rate of procambarus clarkia are reduced, the disease resistance of procambarus clarkia is improved, and the sustainable development of the procambarus clarkia culturing industry is achieved.

The invention relates to procambarus clarkia compound feed, which belongs to shrimp feed. The invention is characterized in that the procambarus clarkia compound feed comprises the following ingredients in percentage by weight: 19 to 22 parts of soybean cake powder, 16 to 19 parts of full wheat powder, 7 to 9 parts of fish meal, 9 to 11 parts of colza cake powder, 9 to 11 parts of cottonseed cake powder, 8 to 10 parts of rice spermoderm, 8 to 10 parts of corn powder, 6 to 8 parts of animal harslet dry powder, 1.5 to 2.5 parts of fish oil, 1.5 to 2 parts of zeolite powder, 1 to 1.5 parts of ground caustic, 1.5 to 2.5 parts of composite mineral substance powder, 1 to 2 parts of premix compound (earthworm, beetle and beer yeast) dry powder, 1 to 1.5 parts of slough powder, 0.1 to 0.3 part of vitamin B complex, 0.05 to 0.15 part of common salt, 0.03 part of antioxidants and 0.05 part of mildew inhibitors. The trial use shows that the invention has the advantages that: 1. the procambarus clarkia compound feed has overall nutritive elements and reasonable mixture ratio, the growth speed of the fed procambarus clarkia is improved by 20 to 30 percent than that of the procambarus clarkia fed with existing randomly prepared feed; and 2. because the palatability is good, the digestive absorption utilization rate is high, and compared with that when feeding the existing randomly prepared feed, the practical culture production cost is reduced by 15 to 25 percent.

The invention discloses a construction method for a micro-ecosystem. The construction method comprises the steps of constructing a micro ecological pond, wherein the depth of the pond is 0.4-0.6m, a mud pile with height of 15-20cm is arranged in one eighth to three eighth area of the bottom of the pond and a sand pile is additionally arranged above the surface of water in the pond; arranging a water supply and drainage system and controlling the depth of water in the micro ecological pond to be 12-14cm; collecting and fermenting human manure; cultivating earthworms and fly maggots: introducing the earthworms and the fly maggots into the micro ecological pond; planting leaf vegetables on the mud pile in spring and summer and planting watercress in autumn and winter; putting loaches, rice field eels, tortoises and Procambarus clarkii respectively according to standards of 20 loaches per square meter, 4 rice field eels per square meter, 4 tortoises per square meter and 1 pair of Procambarus clarkii per 10 square meters. The construction method for the micro-ecosystem is suitable for cultivating the fly maggots and the earthworms by using domestic wastes, the biological wastes are enabled to become green foods of people by cultivating aquatic animals and planting aquatic vegetables, and for the nation, not only can the eutrophication of natural water bodies be prevented, but also the commodity price can be stabilized.

The invention discloses environment-friendly type compound feed of young procambarus clarkii and a feeding method of the environment-friendly type compound feed. In the feed, (imported) fish meal, soybean meal, (imported) rapeseed meal, peanut meal and the like are used as protein raw materials, so that the requirements on amino acids of the procambarus clarkii are met, the utilization rate of the protein is improved and the emission of nitrogen is reduced; the efficiency of the feed is improved and the cost is reduced; inkfish cream, allicin and the like are added so that the feed has a certain food calling effect; the utilization rate of baits by the procambarus clarkii is improved and wastes are reduced; and additives including bacillus, phytase and the like are added so that the feed has the effects of regulating intestinal microorganism flora, improving digestion and absorption of phosphorus and the like. The selected raw materials have sufficient sources, a production process is simple and the feed is not easy to go bad and is convenient to transport and preserve.

The invention relates to a quick-acting environment-friendly pool cleaning method for procambarus clarkia. The quick-acting environment-friendly pool cleaning method comprises the following steps that derris roots are cut into thin slices and are crushed into fine powder after being dried, the sieving is carried out by a bolting silk screen, and derris root powder is prepared; during the procambarus clarkia pool cleaning, about 1-1.5kg of derris root powder is used for 667m<3> of water bodies and is added into a pool to be soaked by clean water, then, the water and the slag are simultaneously and uniformly spread into the pool, and wild trash fishes can be fast killed in 2-4 hours; and after the pool cleaning and sterilization through the derris root powder, fermented organic manure of pigeon manure or rabbit manure can be spread into the water bodies, and shrimp seeds can be placed into the pool after 5-7 days after the fermentation. The natural Chinese herbal medicine plant of the derris is adopted to be prepared into the pool cleaning medicine, the process is simple, the operation is convenient, and compared with the conventional pool cleaning medicine, the prepared derris root powder has the advantages that the effect taking speed is high, the efficiency is high, the medicine dosage is low, the production cost is low, green and environment-friendly effects are realized, the degradation in the water bodies is easy, no medicine residue is left, the derris root powder is safe on pool remaining propagating parent procambarus clarkia, shrimp seeds and waterweeds, and the like. The method provided by the invention is particularly suitable for being used for pool cleaning during the procambarus clarkia culture.

The invention discloses a procambarus clarkia feed additive as well as a preparation method and use thereof, belonging to the technical field of aquatic product feed additives. The procambarus clarkia feed additive comprises the following raw materials in parts by weight: 1 part of honeysuckle, 0.8-1.2 parts of radix astragali, 0.6-1.0 part of poria cocos and 0.7-1.1 parts of houttuynia cordata. The preparation method comprises the steps of weighing 1 part of honeysuckle, 0.8-1.2 parts of radix astragali, 0.6-1.0 part of poria cocos and 0.7-1.1 parts of houttuynia cordata according to parts by weight, respectively soaking, poaching and filtering the raw materials to obtain filtrates, respectively concentrating the filtrates to obtain honeysuckle concentrated solution, radix astragali concentrated solution, poria cocos concentrated solution and houttuynia cordata concentrated solution, mixing the concentrated solutions to obtain the procambarus clarkia feed additive. The procambarus clarkia feed additive has the advantage of remarkably improving the sum of blood corpuscles, the lysozyme, the superoxide dismutase, the acid phosphatase and the alkaline phosphatase of the procambarus clarkia to ensure the healthy growth and reduce the death rate by using the honeysuckle, the radix astragali, the poria cocos and the houttuynia cordata to form the additive.

The present invention discloses a feed for procambarus clarkia. The feed comprises the following raw materials in parts by weight: 85-130 parts of fishmeal, 375-450 parts of soybean meal, 40-70 parts of fermented soybean meal, 90-120 parts of puffed soybeans, 220-260 parts of puffed wheat, 40-60 parts of rice bran, 22-28 parts of calcium dihydrogen phosphate, 4-7 parts of choline chloride, 8-12 parts of compound minerals, 2-5 parts of complex vitamins, 1-1.5 parts of a shelling agent, and 4-6 parts of edible salt. All the components in the feed recipe are natural substances, ensure basic nutrition of the feed for the procambarus clarkia, and at the same time increase effective ingredients of proteins, carbohydrates, phosphorus, calcium, vitamins, minerals, etc., so that the feed for the procambarus clarkia is comprehensive and balanced in nutritional ingredients, effectively enhances immunity, disease resistance, a variety of stress resistance ability of shrimps, strengthens growth of the shrimps, improves survival rate, and are comprehensive and balanced in the nutritional ingredients.

The invention relates to a method for preparing a seasoning by utilizing the zymolysis of the heads of procambarus ckarkii, which pertains to the technical field of aquatic product processing. The method takes the heads of procambarus ckarkii as a raw material and the seasoning product is acquired by removing impurities, concentration and drying after the completion of zymolysis and hydrolysis. The method utilizes the heads of procambarus ckarkii as the raw material, is beneficial to promoting the processing level of the wastes of agricultural products, livestock products and aquatic products of China and can produce great economic benefit.

The invention discloses a preparation method for a procambarus clarkia simple sequence repeats (SSR) primer. The preparation method comprises the steps: (1), extracting procambarus clarkia genome DNAs: extracting the genome DNAs from procambarus clarkia abdominal muscles; (2), hybridizing a probe and a target segment: hybridizing the biotin labeled probe and the target segment to obtain a hybridization product; (3), enriching magnetic beads: enriching the hybridization product by a magnetic bead enrichment method to obtain an enrichment product; (4), performing positive clone sequencing, and designing a primer amplification gene DNA according to a microsatellite flanking sequence; (5), performing SSR primer detection. The preparation method for the procambarus clarkia SSR primer has the advantages that a microsatellite sequence of the procambarus clarkia whole genome can be simply and quickly screened; a large number of SSR primers can be developed at a short time; technical measures are provided for germ plasma conservation and genetic improvement of the procambarus clarkia.

The invention discloses a method for different-position mixed lobster breeding in the same river adult crab aquaculture pond. The mixing breeding method comprises the steps of pond design, juvenile crab capture and stocking, juvenile lobster stocking and capture, lobster density control, feeding management, daily management and river crab capture and marketing. The method is characterized in pond design, juvenile procambarus clarkia density control, lobster larva supplementing at regular intervals and removal of lobsters in a river crab breeding area. By using the ecological breeding method, the yield of lobsters in deep ditches around the pond in per mu of pond is 35-50 Kg/mu, the adult crab yield of the river crab breeding area is about 70 Kg/mu, and the procambarus clarkia yield is about 10 Kg/mu. On the premise that the adult crab breeding cost is not increased basically, the net profit per mu of the river adult crab aquaculture pond is increased by 500-1000 yuan, the culture benefit is increased, and the risk of river crab single variety breeding is reduced.

The invention discloses a method of quickly analyzing group and number of blood lymphocytes of various common crustaceans according to the principles that with the particle structure in the blood lymphocytes of crustaceans, such as litopenaeus vannamei, procambarus clarkia, eriocheir sinensis and the like, as classification reference, the blood lymphocytes, in different groups, can be discriminated and statistic analysis is automatically completed at the same time with a region, where sample points are located, and auxiliary images as evidences by means of a multi-dimensional panorama analyzer having an image auxiliary function. The method is free of dyeing and avoids influence due to manual steps such as normal dyeing, observation under a microscope and the like; by means of automatic signal analysis of a high-sensitivity detector, the method has good repeatability. The multi-dimensional panorama analyzer has high-throughput detection performance, so that the reliability of a result from tens of thousands cell sample points is far higher than that of manual counting. The method is very low in usage amount of reagents and consumables and is low in cost due to trace amount detection. The method not only can be used for scientific analysis and daily monitoring, but also can supply technical support to classifying collection of the blood lymphocytes, so that the method has great application value.

The present invention belongs to the technical field of genetic engineering and discloses a procambarus clarkii C-type lectin gLecB gene and an encoded gLecB protein thereof. A sequence of the procambarus clarkii C-type lectin gLecB gene is shown in SEQ ID NO:1; an amino acid sequence of the gLecB protein is shown in SEQ ID NO:2; and an expression vector is pGEX-5X-1-B-Lectin. The new C-type lectin gLecB is isolated from procambarus clarkii and has an antiviral effect.

The invention discloses a feeding attractant for juvenile procambarus clarkii as well as a preparation method and application of the feed attractant. The feeding attractant comprises the following components in parts by weight: 30-40 parts of lycine, 15-25 parts of kanirin, 15-20 parts of dimethyl-beta-propiothetin and 20-30 of allicin. The preparation method of the feeding attractant comprises the following steps: weighing the components according to the weight parts and then mixing uniformly. The raw materials of the feeding attractant is stable in source and can be taken from a wide range, the production process is simple, the cost is low, and the effect is obvious; from the food intake and digestion mechanism of crustacean, the feeding attractant improves the food intake rate of procambarus clarkia bodies and reduces the waste of baits, simultaneously, the activity of digestive enzymes of the intestines and pancreases of the juvenile procambarus clarkii is improved to promote the growth of the procambarus clarkii.

The invention discloses a tor-brevifilis and procambarus-clarkii rice field mixed aquaculture method. The tor-brevifilis and procambarus-clarkii rice field mixed aquaculture method includes the steps of arranging a rice field, digging annular furrows and field furrows, building field ridges, and arranging protective devices; in February, planting water plants in a layered mode, and putting snails; carrying out disinfection and fertilizing after the mixed aquaculture rice field is cleared, and breeding young procambarus clarkiis at the time one week after fertilizing, wherein the breeding density ranges from 15,000 per mu to 20,000 per mu, and the breeding time is early May; in late-May, breeding tor brevifilises, wherein the density ranges from 1,300 per mu to 1,700 per mu; respectively feeding the tor brevifilises and the procambarus clarkiis, wherein the feeding time of the procambarus clarkiis is sixteen o'clock to eighteen o'clock, the everyday feeding amount is 2% to 3% of the weight of all the procambarus clarkiis, and the everyday feeding amount of the tor brevifilises is 4% to 7% of the weight of all the tor brevifilises; managing the water quality. The tor-brevifilis and procambarus-clarkii rice field mixed aquaculture method is easy to operate and control, the attack rate of the tor brevifilises and the attack rate of the procambarus clarkiis are reduced, the survival rate of the tor brevifilises and the survival rate of the procambarus clarkiis are increased, growing is rapid, and the economic benefits of farmers are high.

The invention discloses a preparation method of Chinese herbal medicine compound fermented feed for procambarus clarkia. The feed is prepared from a fermentation substrate, a bacterial agent and a Chinese herbal medicine compound extract by a multiple fermentation process; the mass ratio of the bacterial agent, the Chinese herbal medicine compound extract and the fermentation substrate is (0.2-5):(0.2-4):50000, and the fermentation substrate comprises, in parts by mass, 5-15 parts of soybean meal, 30-40 parts of wheat, 30-40 parts of corn, 10-15 parts of bran, 1-5 parts of molasses, 1-3 partsof compound premix and 10-20 parts of water; and the Chinese herbal medicine compound extract comprises, in parts by mass, 15-20 parts of yucca, 15-20 parts of cortex eucommiae, 15-20 parts of radix astragali, 10-15 parts of garlic, 10-15 parts of common fenugreek seeds and 10-15 parts of folium mori. The number, activity and survival time of intestinal probiotics in the product are guaranteed, the Chinese herbal medicine compound extract and the compound premix with specific composition have characteristics of micro ecological preparation and functions of antibiotic-like growth factors, the immunity and disease resistance of the procambarus clarkia can be significantly enhanced, the disease incidence can be decreased, and the feeding quality can be improved.

The invention discloses a Procambarus clarkii astacidin antibacterial peptide gene and coded antibacterial peptide thereof, and also discloses application of the Procambarus clarkii astacidin antibacterial peptide gene in preparing recombinant protein with antibacterial activity. The antibacterial peptide of recombinant Procambarus clarkii astacidin obtained through gene expression can be used in antibacterial feed additives, food preservation, animal-plant gene transformation, drug development and other fields.