The invention discloses a method for producing a nano-MV-MOF-based fluorescent enhanced test paper for biomarker detection. The method comprises the following steps: preparing a suspension from a nano-molecular tweezer-metal organic framework material and a metal ion coordination bonding material, uniformly coating a chromatography paper with the suspension, and drying the coated chromatography paper to obtain the test paper. The nano-MV-MOF material used in the test paper has an ultrahigh specific surface, uniformly-distributed host-guest interaction sites and a well-regulated host-guest interaction hole chemical environment, has excellent detection performances and can be repeatedly used. The test paper has the advantages of simplicity in production, easiness in implementation, and specific detection property and very low detection limit for a biomarker mercaptoamino-acid, can be reused after being simply processed, and can be widely applied to the field of health detection.