9 results about "Interaction site" patented technology

The invention discloses a wheat scab disease-resistant protein TaSnRK1alpha and fusarium graminearum secretory protein Osp24 interaction key site and application thereof, and relates to the technical field of bioengineering. According to the invention, TaSnRK1alpha protein structural domain distribution and sequence characteristics are analyzed, the TaSnRK1alpha protein is divided into multiple forms, and a yeast two-hybrid test is utilized to identify that the 479th valine of the TaSnRK1alpha protein is a key site for determining interaction with secretory protein Osp24. After the sites are mutated, the targeting of the fusarium graminearum secretory protein Osp24 is blocked. Therefore, an ideal candidate editing target spot is provided for accurately modifying TaSnRK1 alpha by utilizing a CRISPR-Cas9 technology, the targeting and inhibition of fusarium graminearum on TaSnRK1 alpha can be effectively avoided, the gibberellic disease resistance is improved, and a gibberellic disease resistant wheat material is created.

The invention provides a polypeptide for enhancing T cell function and application thereof, and belongs to the technical field of biological medicine. The amino acid sequence of the polypeptide disclosed by the invention is as shown in SEQ ID NO. 1. According to the invention, an interaction interface of CLDN18.2 and beta-catenin is targeted for the first time, the full D-type functional polypeptide is designed to realize high-stability and high-affinity protein-protein interaction blocking, and the problem that the interaction site is difficult to target by the traditional antibody drug or small molecule drug is broken through; the used polypeptide PC18.1 has good in-vitro stability and is suitable for subsequent clinical development; the polypeptide is easy to prepare on a large scale through a synthesis means, has the purity of more than or equal to 98%, is low in cost and convenient to modify, and can be flexibly applied to immune combined treatment schemes such as nano-carrier loading and local delivery.

A track arrangement (1) for self-driven carriages (2) in a storage rack arrangement (3) stores and accessed objects (4). A plurality of storage sites (5) are arranged in a rack extending over k storage levels (7). The track arrangement connects each storage level (7) with at least one interaction site (10) for processing stored objects and / or issuing objects to be stored and includes Z track levels, Z≥k, an entry point (27) on the mth track level for each interaction site (10), mϵ{1, . . . , Z}, and an exit point (28) on the nth track level for each interaction site, n∈{1, . . . , Z}. Each interaction site has: Z−m first one-way ramps (14) directed downward towards the entry point, m−1 second one-way ramps (16) directed upward towards the entry point, Z−n third one-way ramps (20) directed upward away from the exit point, and n−1 fourth one-way ramps (22) directed downward away from the exit point.

The present invention provides a method for detecting nucleic acid chemical modification sites and interaction sites based on nucleic acid grafting and nucleic acid reverse transcription template conversion technology. Before detecting the nucleic acid chemical modification sites and interaction sites, an RNA branch chain needs to be obtained; then, using a reverse transcription primer that is complementary to the RNA branch chain, reverse transcription is carried out starting from the RNA branch chain. When the reverse transcription proceeds to the junction of the RNA branch chain and the modification or the interaction junction, grafting template conversion is performed, and the main chain connected to the RNA branch chain is used as a template to continue reverse transcription to obtain cDNA containing modification site or interaction site information; finally, a high-throughput library is constructed using the cDNA containing modification site or interaction site information and high-throughput sequencing is carried out to determine the modification site or interaction site; or the cDNA containing modification site or interaction site information is detected by PCR and TA-cloning technology, so as to realize the identification of the modification site or interaction site. This method can be applied to a variety of gene sequencing methods.

The invention discloses an interpretable adversarial training-based circular RNA and RBP interaction site prediction method and an interpretable adversarial training-based circular RNA and RBP interaction site prediction system, relates to the technical field of biological information, and discloses the interpretable adversarial training-based circular RNA and RBP interaction site prediction method and the interpretable adversarial training-based circular RNA and RBP interaction site prediction system. Through multi-scale feature coding, context correlation feature extraction, an adversarial training mechanism and a motif analysis technology, the problems of insufficient feature integration, poor model robustness and lack of biological interpretability in an existing method are solved, and the method has the advantages of effectively improving the prediction precision, enhancing the anti-interference capability of the model and providing an interpretable result.

The invention discloses a transportation path planning method and system based on a weighted directed graph. The method comprises the steps of querying a process path bound with a to-be-transmitted object to determine a target interaction platform; an interaction platform where the to-be-transmitted object is located currently is obtained; according to the target interaction station and an interaction station where the to-be-transmitted object is currently located, in a pre-created weighted directed graph, a transportation path of the to-be-transmitted object is dynamically calculated and transmitted by using a shortest path algorithm until the to-be-transmitted object reaches a target station; and when the to-be-conveyed object arrives at the target station, the to-be-conveyed object is conveyed into the process equipment. By implementing the method provided by the invention, a system capable of dynamically planning the logistics path according to the actual production condition can be realized, and the path can be adjusted in real time to cope with the change of the process sequence, so that the flexibility, response speed and efficiency of the production process are improved.

The application belongs to the technical field of biotechnology and three-dimensional genomics, and discloses a capture probe for targeting a promoter long-range interaction site of a whole pig genome, a kit and application thereof. The technology can efficiently enrich and accurately detect the regulatory elements in the pig genome which have long-range interaction with the promoter, significantly improves the feasibility and reliability of the long-range interaction research of the pig genome, and provides important technical support for the functional research of the pig genome, the genetic mechanism analysis of complex traits, and the innovation of molecular breeding technology.

The invention refers to a computer implemented method for testing active ingredients with respect to an interaction strength with a target protein structure. For candidate active ingredients a digital twin is received. A digital twin associated with a molecular cut-out of a target protein structure is received. Conformations associated with the candidate active ingredients are determined based on the digital twin associated with the respective candidate active ingredients. An energy measure for the candidate active ingredients is determined based on the conformations and the digital twin associated with the molecular cut-out, wherein the energy measure is indicative of the interaction strength between an active ingredient interacting at the interaction site with the target protein structure. Based on the determined energy measure, digital twins associated with one or more of the candidate active ingredients for further testing with respect to one or more application goals.