Method for producing nano-MV-MOF-based fluorescent enhanced test paper for biomarker detection

A technology of test paper and nano-molecules, applied in the field of preparation of fluorescence-enhanced test paper, achieving low detection limit, easy preparation, and wide application

Active Publication Date: 2018-09-28
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecular tweezers metal-organic framework materials have a specific surface area up to 2000m 2 / g, while the traditional small molecule materials and metal nanoparticle materials have no specific surface area, even the traditional porous material activated carbon is only 800m 2 / g

Method used

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  • Method for producing nano-MV-MOF-based fluorescent enhanced test paper for biomarker detection
  • Method for producing nano-MV-MOF-based fluorescent enhanced test paper for biomarker detection
  • Method for producing nano-MV-MOF-based fluorescent enhanced test paper for biomarker detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Nanoscale molecular pincer metal-organic frameworks (MV-MOFs) and metal ions (Cu 2+ ) preparation of coordination binding materials

[0023] (1) Preparation of nanoscale MV-MOFs

[0024] 1) Add 27mg ZrCl to a 10mL test tube 4 , 30mg TBPA, 20mg MTBC, 123.0mg isonicotinic acid (or other regulatory ligands in equimolar amounts) and 3.4mL N,N-diethylformamide. Plug the test tube with a rubber stopper and insert it into a sand bath, stir at a speed of 200r / min, heat at 120°C for 24 hours, cool to room temperature, and centrifuge to obtain a powdery solid, that is, MV-MOFs. figure 1 , scanning electron microscopy showed that the size of MV-MOFs is about 500nm.

[0025] The aforementioned equimolar amounts of regulatory ligands can also be 54.7 μL formic acid, 107.7 μL trifluoroacetic acid, 175.0 mg benzoic acid, 225.5 mg p-chlorobenzoic acid and 220.2 mg p-nitrobenzoic acid. The regulatory ligand used in the nanoscale MV-MOFs in the following experiments and examples is i...

Embodiment 2

[0031] Preparation of fluorescence-enhanced test paper for biomarker detection based on nanoscale MV-MOFs

[0032] (1) Get activated nanoscale MV-MOFs and Cu in Example 1 2+ 0.36 g of the coordination binding material was dispersed in 10 mL of deionized water to prepare a 15 mM suspension of the coordination binding material.

[0033] (2) Spread the aluminum oxide chromatography paper on the cardboard with a glass rod with ferrules at both ends, control the thickness to 1mm, then take 25 μL of the suspension of the coordination binding material, and drop it evenly on the 2cm× On a 3 cm chromatographic paper, the evenly coated chromatographic paper was dried at 60°C for 24 hours to obtain a fluorescence-enhanced test paper for biomarker detection based on nanoscale MV-MOFs.

Embodiment 3

[0035] Detection of thiol amino acids

[0036] (1) Using nanoscale MV-MOFs materials for the detection of thiol amino acids

[0037] Use fluorescence titration to make a standard working curve: the instrument model used in the fluorescence titration experiment is: Agilent CaryEclipse. First, 1.0 mg of activated nanoscale MV-MOFs material was dispersed in 2 mL of deionized water by ultrasound. Next, select an excitation light source of 415 nm, under the conditions of this excitation light source, drop 1 mM copper nitrate solution into the original solution with 10 μL per drop, and record the fluorescence intensity change curve until the intensity no longer decreases. Then, 1 mM cysteine ​​solution was dropped into the original solution with 10 μL per drop, and the fluorescence intensity change curve was recorded until the intensity no longer decreased. Take the cysteine ​​concentration as the abscissa and the intensity as the ordinate to make a standard working curve. For ob...

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Abstract

The invention discloses a method for producing a nano-MV-MOF-based fluorescent enhanced test paper for biomarker detection. The method comprises the following steps: preparing a suspension from a nano-molecular tweezer-metal organic framework material and a metal ion coordination bonding material, uniformly coating a chromatography paper with the suspension, and drying the coated chromatography paper to obtain the test paper. The nano-MV-MOF material used in the test paper has an ultrahigh specific surface, uniformly-distributed host-guest interaction sites and a well-regulated host-guest interaction hole chemical environment, has excellent detection performances and can be repeatedly used. The test paper has the advantages of simplicity in production, easiness in implementation, and specific detection property and very low detection limit for a biomarker mercaptoamino-acid, can be reused after being simply processed, and can be widely applied to the field of health detection.

Description

technical field [0001] The invention relates to the field of detection test paper, in particular to the preparation of a fluorescence-enhanced test paper for biomarker detection based on a nano-molecular pincer metal-organic framework material (MV-MOF). Background technique [0002] Test paper is widely used in various fields due to its unique characteristics of low detection cost and simple operation, such as pH test paper and starch potassium iodide test paper used in laboratories; temperature-sensitive test paper used in industry and waste water detection test paper; blood sugar test paper used in daily life Wait. Due to the different materials and methods of making the test paper, the detection field and detection sensitivity of the test paper are also different. Existing detection test paper is mostly made of organic small molecules and nanoparticles with almost no specific surface area, for example, in 2006, Bai et al. (Z.Lin, S. Ou, C.Duan, B.Zhang, Z.Bai.Chem.Commun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 邓鹤翔汪洋刘琦张勤彭博思
Owner WUHAN UNIV
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