435 results about "Nitrobenzoic acid" patented technology

The invention relates to a method for performing supersensitive detection on fungaltoxin of a DTNB mark-based gold@silver core-shell nanorod, and belongs to the technical fields of food safety, environment monitoring and the like. The gold nanorod is prepared by a seed growing method, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) is marked on the gold nanorod, meanwhile, the gold nanorod is coated with a silver shell layer to prepare a gold@DTNB@silver surface Raman enhanced substrate, a superparamagnetic magnetic material chitosan ferroferric oxide is prepared, a fungaltoxin aptamer complementary chain is coupled on the prepared Raman enhanced substrate, a fungaltoxin aptamer chain is coupled on the magnetic material, an enhancer and the magnetic material are combined and a Raman signal of a system is strongest when the fungaltoxin does not exist in the detection system, and the aptamer modified magnetic material is specifically and preferentially combined with the fungaltoxin and the Raman signal of the system is changed after an external magnetic field is separated when the fungaltoxin exists, so that the aim of quantitatively detecting the fungaltoxin is fulfilled.

The invention relates to a method for preparing 4-(3-chlorine-4-fluorophenylalanine)-7-methoxy-6-[3-(4-morpholinyl) propoxy] quinazoline (I). The method takes 4-methoxy-2-nitrobenzoic acid as initial material which is subjected to reduction and nitration to obtain 4-methoxy-5-nitryl-2-aminobenzoic acid, is then subjected to cyclization to obtain 7-methoxy-6-nitryl quinazoline-4(3H)-ketone, is again subjected to reduction and diazo reaction hydrolysis to obtain 7-methoxy-6-hydroxy quinazoline-4(3H)-ketone, after etherification reaction is carried out, phosphorus oxychloride is used for preparing 7-methoxy-6-[3-(4-morphjolinyl) propoxy]-4-chloroquinazoline, which is subjected to amination to obtain the compound in the formula (I). The initial material adopted by the preparation method is convenient and accessible, the cost is low, the process route is simple and reasonable, the three wastes produced in the process of preparation generates less pollution, therefore the preparation method can prepare final product with high quality and yield in mass production, and is suitable for industrialized production.

The invention relates to the chemical field and in particular to an enzyme inhibition method for detecting carbamates. Ethyl carbamate is oxidized by using an oxidant in the presence of an acetylcholine iodide substrate, a 5,5'-dithio bis-(2-nitrobenzoic acid) (DTNB) developer and an acetylcholin esterase with the activity of more than 200U/g, and then the absorbance variation value of the ethyl carbamate along with time at 3min is detected by spectrophotometry to obtain the inhibition condition of the ethyl carbamate on the acetylcholin esterase so as to obtain the content of the ethyl carbamate. The substrate and the developer used in the invention are low in cost; the inhibition rate is greatly increased since the ethyl carbamate is oxidized by using the oxidant; and the method is rapid, simple, high in sensitivity, mild and easily controlled in reaction conditions, simple in steps and easy to operate and needs less equipment. According to the principle of the method, a rapid kit is developed for the rapid detection of ethyl carbamates in alcoholic beverages and fermented foods.

A toxic material detection apparatus includes a sample collection portion including a sample inlet and a sample concentrator adapted to concentrate an environmental sample on a substrate. A sample distributing system transfers portions of the substrate to a color sensor and an ion mobility spectrometer for simultaneously analysis and toxin detection, particularly cholinesterase inhibitor detection. Optionally, a portion of the substrate may be directed to an archive for possible analysis at a later time. Reagents utilized include the enzyme acetylcholinesterase (AChE), and the reactants acetylthiocholine iodide (ATCI) and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTB). A data management unit provides for near-real-time analysis of the samples in under 5 minutes. Simultaneous “hits” by both analysis methods indicate the presence of a cholinesterase inhibitor.

The invention relates to a method for growing a silver nano-film on copper material, which belongs to the field of inorganic nano-material preparation. The method uses the replacement reaction between elemental copper and silver ions, takes nitrobenzoic acid as a control agent, and covers a layer of silver nano-film on the surface of the copper material. The method has the advantages of reasonable design, simple operation, easily controllable reaction, good repeatability and easy large-scale production. The silver nano-film has the advantages of uniform size, high distribution density, and very high conductivity and inoxidability. The method can be used in SERS, electronics industry and other fields.

The invention provides a surface-enhanced Raman detection method for mycotoxin based on a silica-coated gold nanotriangle. According to the method, a three-step seed induction method is employed for preparation of a gold nanotriangle, and the gold nanotriangle is labeled with a 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) signal molecule and coated with silica so as to prepare a gold@DTNB@silica surface Raman enhancing agent; a chitosan ferriferrous oxide (CS-Fe3O4) magnetic material is prepared at the same time; an enhanced substrate and the magnetic material are modified with mycotoxin aptamer so as to construct a mycotoxin detection system; when mycotoxin exists, gold@DTNB@silica and CS-Fe3O4 are bonded together via the effect of the specific recognition of the aptamer so as to form a CS-Fe3O4-aptamer-mycotoxin-gold@DTNB@silica detection system; with changes of the concentration of mycotoxin, the Raman signal of the system is not changed after magnetic separation; and thus, quantitative ultra-sensitive detection of mycotoxin in food is realized. The method is applicable to the technical fields of food safety, material chemistry and the like.

The invention relates to low silicon content coolant liquid applicable to an aluminum radiator of a heavy-duty commercial vehicle engine, and belongs to coolant liquid. The coolant liquid comprises the following raw materials in portion by weight: 36 to 52 portions of terylene grade glycol, 1.0 to 1.5 portions of mannite, 0.3 to 0.7 portion of phenylformic acid, 0.7 to 0.9 portion of adipate, 0.1 to 0.2 portion of methylbenzotriazole, 0.05 to 0.1 portion of p-nitrobenzoic acid, 0.001 to 0.004 portion of polyether, 0.3 to 0.7 portion of potassium phosphate, 0.2 to 0.25 portion of sodium nitrite, 0.25 to 0.3 portion of sodium nitrate, 0.5 to 1.0 portion of borax, 0.007 to 0.02 portion of sodium silicate, 0.45 to 0.68 portion of sodium hydroxide, and distilled water for fixing the volume to 100 portions. The coolant liquid contributes to improvement of heat exchange and cooling performance of a water jacket of the engine, is more adapted to an aluminum water tank radiator, is adapted to sedans and light vehicles, and is more adapted to heavy-duty commercial vehicles.

The invention discloses a polymethylmethacrylate composite material for 3D printing and a preparation method thereof. The preparation method comprises the following steps: dissolving acrylic acid in ethyl acetate, adding zaodiisobutyronitrile, placing at room temperature, then sequentially adding paranitrobenzoic acid and isobutyl cyanacetate, stirring at room temperature, subsequently adding polymethylmethacrylate particles and continuously stirring at room temperature to obtain the polymethylmethacrylate composite material for 3D printing. The polymethylmethacrylate composite material comprises 30-60 percent of polymethylmethacrylate, 10-30 percent of isobutyl cyanoacetate, 1-10 percent of crylic acid, 20-40 percent of ethyl acetate, 1-2 percent of zaodiisobutyronitrile, and 1-10 percent of paranitrobenzoic acid. The composite material prepared according to the preparation method can be used for 3D printing within the temperature range of 10-35 DEG C, and the polymethylmethacrylate composite material for 3D printing and the preparation method thereof are easily popularized and applied due to simple preparation process, easy availability of the raw materials and low production cost.

The invention provides a method for synthesizing 2-chloro-5-nitrobenzoic acid through a microchannel reactor. The method comprises the steps of dissolving a raw material o-chlorobenzoic acid into concentrated sulfuric acid to obtain a material 1, and entering a preheating module; adopting fuming nitric acid and the concentrated sulfuric acid as a material 2 and a material 3, and entering another preheating module; preheating the material 1, the material 2 and the material 3, entering a reaction module group for reacting, collecting effluent reaction liquid, processing to obtain a crude product, and refining to obtain the product 2-chloro-5-nitrobenzoic acid. According to the method provided by the invention, the reaction time is shortened to a few minutes to a few seconds, the production energy consumption is reduced, and the reaction efficiency is remarkably improved. High-efficient mass and heat transfer efficiency ensures the reaction temperature to maintain within a setting range, the possibilities of temperature out of control, temperature runaway and even sharp reaction overflow or explosion caused by over-high local concentration do not exist, the intrinsic safety problem of the nitration reaction is solved fundamentally, and the reaction yield and the product purity are remarkably improved.

The invention belongs to the technical field of polymer drugs, and particularly relates to a long-circulating target photosensitive antitumor drug conjugate and a preparation method of the long-circulating target photosensitive antitumor drug conjugate. The preparation method comprises the following steps: firstly, carrying out amidation reaction to a single-end amino polyethylene glycol amino with ligand functionalization and 4-(1-ethoxy)-2- methoxy group-5-nitrobenzoic acid containing a photosensitive group; then carrying out esterification reaction with an esterifying agent; and finally coupling with the antitumor drug containing the amino to obtain the long-circulating target photosensitive antitumor drug conjugate. According to the conjugate prepared by the preparation method, the circulating time of the drug in the body can be prolonged, tumor tissues are accelerated to adsorb drug, and the conjugate has the target to quicken the enrichment speed of the antitumor drug in the tumor tissues. The conjugate is fractured by the photostimulation of specific wavelength to release original drug and quickly achieve treatment concentration, and 'time/space' controllable effective treatment is obtained. Meanwhile, the preparation method provides a simple and effective path for preparing the target controllable photosensitive biological polymer drug.

The present invention discloses a preparation method of photoresponsibility azobenzene graft carbon nano tube material, belonging to preparation technology of optical, electric and energy-transducing material. Said preparation process includes the following steps: adding multiwall or single-wall carbon nano tube into mixed acid formed from nitric acid and sulfuric acid to make acidification, dispersing acidified carbon nano tube in the SOCl2 solution to make reaction to obtain carbon nano tube with acyl chloride function group; mixing nitrodracylic acid and zinc powder in sodium hydroxide to obtain para-dihydroxyazobenzene, then mixing it and sulphoxide dichloride in chloroform to obtain p-4,4'-amide linear alkylamine azobenzene, adding p-4,4'-amide linear alkylamine azobenzene and nano tube with acyl chloride function group into tetrahydrofuran, heating and reacting for above 96h, dewatering, evaporating to obtain paste-like material, dispersing it in chloroform, filtering and drying so as to obtain the invented product.

The invention discloses a method for preparing acetovanillone and acetosyringone (AS) through oxidation of lignin by using an oxidizing agent. According to the method, an oxidizing agent reacts with the lignin in an alkaline solution; the resulting reaction solution is subjected to acidification, extraction and concentration to obtain the crude product after completing the reaction; the treatments of recrystallization and rectification are performed to obtain the acetovanillone and the AS. The oxidizing agent is the one selected from p-nitrobenzoic acid, 3,5-dinitrobenzoic acid, 3-nitrosalicylic acid, 5-nitrosalicylic acid or 3,5-dinitrosalicylic acid. According to the present invention, the oxidizing agent has low toxicity, such that the harm to the environment can be reduced; the post-treatment steps are simplified, and the uses of the organic solvents are reduced, such that the secondary pollution to the environment is reduced; the yield is relatively high, the purities of the products are respectively 97.3% and 98.2% through the gas chromatography analysis; the two important chemical raw materials of guaiacol and lilac alcohol can be synchronously obtained when preparing the acetovanillone and the AS.

The invention relates to a preparation method and applications of a hyperbranched azo polymer, belonging to the organic synthesis field. The preparation method comprises the steps: firstly, performing nitroreduction and carboxyl acylation by taking p-nitrobenzoic acid as a raw material, to obtain an A2 type monomer azo-bi(benzoyl chloride) with an azobenzene structure, and then reacting the A2 type monomer azo phthaloyl dichloride with a B3 type monomer glycerol according to certain mole ratio to obtain the hyperbranched azo polymer. The materials of the obtained hyperbranched azo polymer has higher thermo-optical coefficient which is more than ten times of that of inorganic materials such as borosilicate glass, zinc silicate glass, silica glass and the like, and the material provides a possibility for developing a novel digital thermo-optical switch with low driving power.

The invention discloses a preparation method for 4-chloro-6,7-bis(2-methoxyethoxy)quinazoline. The process flow of the method is as follows: A, 3,4-bis(2-methoxyethoxy)benzaldehyde undergoes nitration to produce 4,5-bis(2-methoxyethoxy)-2-nitrobenzaldehyde; B, oxidation is carried out so as to obtain 4,5-bis(2-methoxyethoxy)-2-nitrobenzoic acid; C, esterification is carried out so as to obtain 4,5-bis(2-methoxyethoxy)-2-nitrobenzoate; D, reduction is carried out so as to obtain 4,5-bis(2-methoxyethoxy)-2-aminobenzoate; E, cyclization is carried out so as to obtain 6,7-bis(2-methoxyethoxy)-4(3H)-quinazolinone; and F, chlorination is carried out so as to obtain 4-chloro-6,7-bis(2-methoxyethoxy)quinazoline. The preparation method provided in the invention has the advantages of a concise and practical synthetic route, high yield and a good industrial application prospect.

The invention discloses a preparation method of 3-methyl-2-nitrobenzoic acid. 3-methylbenzoic acid alkyl ester is used as raw material in nitrification, two-grade selectivity and high yield are realized, and the amount of waste acid was reduced. In nitrification product, only 3-methyl 2-nitrobenzoic acid alkyl ester and 3-methyl-4-nitrobenzoic acid alkyl ester are hydrolyzed after separation to obtain 3-methyl 2-Nitrobenzoic acid and 3-methyl-4-nitrobenzoic acid. The process is simple, and suitable for industrial production.

The invention relates to the field of production of chemical engineering products, in particular to a method for synthesizing p-nitrobenzoic acid. The method comprises the following steps of using p-nitrotoluene as the raw material, using diluted nitric acid as a reaction medium and a catalyst, using oxygen as an oxidant, and oxidizing in a high-pressure kettle, so as to prepare the p-nitrobenzoicacid; cooling, separating crystal form solid in the reaction liquid, sucking and filtering, so as to obtain a mother liquid and filter cake; spraying the filter cake by water, and drying, so as to obtain a light yellow product, namely p-nitrobenzoic acid. The method for synthesizing the p-nitrobenzoic acid has the advantages that the production purity is high, the preparation is simple, the costis low, the pollution is avoided, and the green effect is realized.

The invention discloses a clean production method to oxidize 3-nitro-o-xylene into 2-methyl-3-nitrobenzoic acid. The clean production method comprises the steps of adding 3-nitro-o-xylene with an acidinto an organic solvent under the action of a catalyst, adding an oxidant, and stirring to allow reacting to obtain 2-methyl-3-nitrobenzoic acid, wherein the oxidant is hydrogen peroxide, and the catalyst is a transition metal compound. The hydrogen peroxide is used herein as a clean oxidant, so that the oxidants, such as potassium permanganate, causing high environmental pollution are avoided. The reaction system is mild; post-reaction treatment is simple and practical; reaction selectivity is good; few byproducts are produced; the target product is easy to purify; both the yield and purityare high; the cost is low; the clean production method has a good industrial application prospect.

The invention relates to an electroreduction preparation method of 2-amino-5-methoxybenzoic acid derivatives represented by a formula I shown in the specification. A preparation reaction is shown in the specification, wherein n is selected from 1, 2 or 3; R is selected from hydrogen, methyl, ethyl or benzyl; Y is selected from C6H5, HO, Cl, Br, mesyloxy, p-toluenesulfonyloxy or M is selected fromCH or N; and W is selected from CH2, O, S, NH, HOCH, BocN, MeN, EtN, C6H5N, 4-ClC6H4N or 4-HOC6H4N. The electroreduction preparation method for the 2-amino-5-methoxybenzoic acid derivatives represented by the formula provided by the invention is characterized by comprising the step of performing constant-current or constant-voltage electrolysis in a diaphragm electrolytic cell by using an acid solution of a 5-methoxy-2-nitrobenzoic acid derivative as a catholyte and an acidic solution as an anolyte, wherein the voltage of a cathode working electrode is 1.00-2.50 V relative to that of a reference electrode, the current density of constant-current electrolysis is 25.0-250.0 mA/cm<2>, and the electrolysis temperature is 15-80 DEG C.

The invention discloses a copper complex ferroelectric functional material and a preparation method thereof. A molecular formula of a copper complex is [Cu(phen)(H2O)2L]L, wherein L is nitrobenzoic acid; phen is phenanthroline; the ferroelectric functional material is dark blue monoclinic powder crystals and belongs to a P21 space group crystal structure, and the purity is not less than 99 percent; a cell parameter of the ferroelectric functional material is that: beta is 97.75(3) degrees; and ferroelectric characteristic parameters of the ferroelectric functional material are respectively that: Ps is 0.218mu C/cm<2>, 2Pr is 0.189mu C/cm<2>, and 2Ec is 5.06kV/cm. The saturated polarization Ps of the ferroelectric functional material is 0.218mu C.cm<-2>. The preparation method for the copper complex has the advantages that the flow and the process are simple, equipment requirements are low, cheap copper salt, phenanthroline and nitrobenzoic acid are used as raw materials, the pollution is avoided, the cost is low, the industrialization is easy to realize, and the like.

The invention discloses an SERS-based newcastle disease virus detection kit and a detection method thereof, and belongs to the field of spectroscopy and molecular diagnosis. The SERS-based newcastle disease virus detection kit comprises a raman probe solution and a nitrocellulose film; the raman probe solution is a solution obtained by coupling nano gold, 3,3'-dithio di(6-nitrobenzoic acid) di-succinic acid imido ester complex and a newcastle disease antibody. The detection method comprises the following steps: dropping a newcastle disease virus on the nitrocellulose film, incubating the nitrocellulose film and the raman probe coupled with the antibody after closing and washing to form an immune complex with a 'solid phase antigen-labeled antibody' half sandwich structure on the nitrocellulose film, and finally performing raman spectrum detection on the immune complex so as to identify an enhanced raman molecular signal of a material to be identified with high specificity. The detection kit and the detection method which are disclosed by the invention are high in specificity, and the detection limit of the newcastle disease virus is 10<3>PFU/mL, and the sensitivity is high.

The invention discloses a formula of metal machining liquid, comprising the following components in percentage by weight: 40-50% of pentaerythritol oleate, 5-10% of lauryl sodium sulfate, 3-8% of dodecyl polyoxyethylene ether sodium sulfate, 1-3% of emulsified silicon oil, 0.5-2% of sodium silicate, 0.5-1% of nitrobenzoic acid and the balance of water. The metal machining liquid has the characteristics of good cooling property, good lubricating property, antirust property, oil removing and cleaning function and anti-corrosion function and is easy to dilute.