462 results about "Near infrared fluorescence" patented technology

A near infrared fluorescent contrast agent comprising a compound having three or more sulfonic acid groups in a molecule, and a method of fluorescence imaging comprising introducing the near infrared fluorescent contrast agent of the present invention into a living body, exposing the body to an excitation light, and detecting near infrared fluorescence from the contrast agent. The near infrared fluorescent contrast agent of the present invention is excited by an excitation light and emits near infrared fluorescence. This infrared fluorescence is superior in transmission through biological tissues. Thus, detection of lesions in the deep part of a living body has been made possible. In addition, the inventive contrast agent is superior in water solubility and low toxic, and therefore, it can be used safely.

According to one embodiment of the invention, a method for biomedical imaging includes directing time-varying excitation light at a surface area of a light scattering material, the material comprising a fluorescent target. Time-varying emission light from the fluorescent target is detected, substantially at a two-dimensional sensor surface, in response to the time-varying excitation light stimulating the fluorescent target. The time-varying emission light is filtered to reject excitation light re-emitted from the material. A three-dimensional image of the fluorescent target is generated based on the detection substantially at the sensor surface.

Disclosed are nanostructured gold films which may be produced by solution-phase depositions of gold ions onto a variety of surfaces. The resulting plasmonic gold films are used for enhanced spectroscopic-based immunoassays in multiplexed microarray format with detection mechanisms based on either surface-enhanced Raman scattering or near-infrared fluorescence enhancement. The preparation of the films and subsequent modifications of the gold film surfaces afford increased sensitivity for various microarrays. The films are discontinuous, forming gold “islands.” Sensitivity, size, shape, and density of the nanoscopic gold islands comprising the discontinuous nanostructured gold film are controlled to enhance the intensity of Raman scattering and fluorescence in the near-infrared, allowing for improved measurements in clinical diagnostic or biomedical research applications.

The invention relates to a method based on near infrared fluorescence molecules and nano particle marks as well as an immunochromatography quantitative detection reagent based on near infrared fluorescence nanoparticles. According to the invention, infrared fluorescence is connected with nanoparticles to prepare an immunochromatography test strip based on the near infrared fluorescence nanoparticles. During the detection, an infrared light scanner is utilized, and a quality control line and a sample line are respectively scanned by utilizing near infrared light; and after the fluorescence intensity of a detection line is rectified by utilizing the fluorescence intensity of a quality control line, and a standard curve in a fluorescence analyzer is taken in, the concentration of a to-be-detected matter in a sample can be analyzed and detected. Compared with the direct connection of the near infrared fluorescence molecules and detecting molecules, the near infrared fluorescence nanoparticle immunochromatography improves the detection flexibility obviously, and the lowers the background fluorescence intensity. The marking method and the reagent can be applied to microorganism detection, food safety detection, poison detection as well as rapid dangerous chemical product detection.

An intramolecularly-quenched, near infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or both. Also disclosed are methods of using the intramolecularly-quenched, near infrared fluorescence probes for in vivo optical imaging.

The invention discloses a flow cytometry. The flow cytometry comprises a light source irradiation system, a liquid flow system, a light split system, a signal detection, analysis and processing system, and a personal computer (PC) display control system; the liquid flow system comprises a flowing chamber; linearly flowing cells are accommodated in the flowing chamber; a solid laser is used as an excitation light source of the light source irradiation system, and laser passes through a reflecting mirror and a plano-convex lens to be converged and irradiated on the flowing chamber, is integrated and then is further converged on the optical part of the flowing chamber to be irradiated on the linearly flowing cells; the signal detection, analysis and processing system comprises a lateral scattering detector, a yellow fluorescence channel detector, a near-infrared fluorescence channel detector, a forward scattering detector and a signal processing module; and all detectors are multi-pixel photon counters (MPPC). The flow cytometry has the characteristics of large dynamic detection range, high sensitivity, small size and compactness and convenience in maintenance.

The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a pteroyl ligand that binds to a target receptor protein, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid or derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.

The invention provided medical apparatuses incorporating dyes to facilitate proper positioning in patients, and methods for their use. The apparatuses can be, for example, tubes, catheters, or needles. In some embodiments, the dye is a near infrared fluorescent dye.

An intramolecularly-quenched, near-infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near-infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or bothours. Also disclosed are methods of using the intramolecularly-quenched, near-infrared fluorescence probes for in vivo optical imaging.

The invention relates to a fluorescence nanometer probe, comprising an inner core formed by polyglycolide lactide, a middle layer formed by phospholipid surrounding on the surface of the inner core and a shell formed by distearoyl phosphatidyl ethanolamine-polyethylene glycol which contains amino or carboxyl and partially penetrates through the middle layer, wherein indocyanine green is dispersed in the inner core. According to the invention, a core-shell structure is formed to ensure that the indocyanine green is wrapped in the polyglycolide lactide, therefore, the indocyanine green is effectively avoided from being aggregated and decomposed and the stability is increased; and the wrapped indocyanine green has a near-infrared fluorescence characteristic to ensure that the background fluorescence penetrating tissues is small and then can be relatively accurately applied to bioluminescence labeling.

Disclosed are methods for imaging lumen-forming structures such as blood vessels using near-infrared fluorescence in the NIR-II region of 1000-1700 nm. The fluorescence is created by excitation of solubilized nano-structures that are delivered to the structures, such as carbon nanotubes, quantum dots or organic molecular fluorophores attached to hydrophilic polymers. These nanostructures fluoresce in the NIR-II region when illuminated through the skin and tissues. Fine anatomical vessel resolution down to −30 μm and high temporal resolution up to 5-10 frames per second is obtained for small-vessel imaging with up to 1 cm penetration depth in mouse hind limb, which compares favorably to tomographic imaging modalities such as CT and MRI with much higher spatial and temporal resolution, and compares favorably to scanning microscopic imaging techniques with much deeper penetration.

The invention belongs to the technical field of optical nano probe, and in particular, relates to a photoluminescence-photothermal nano composite structural material and a preparation method and an application thereof. The composite structural material takes a rare earth fluoride as a core and is a core-shell structural nano material with the exterior wrapped with an organic photothermal substance layer; the material generates 800-1200 nm near infrared fluorescence emission under excitation by a 700-1000 nm near infrared laser, the photo-thermal substance layer can convert optical energy into heat energy to achieve photothermal therapy of tumor. During use, the probe temperature is monitored while the probe performs photothermal therapy; furthermore, the probe is co-incubated with tumor cells or is injected into tumor mice through veins, and high-resolution cancer photothermal therapy and simultaneous optical temperature monitoring can be achieved. The invention provides an effective temperature monitoring tool for photothermal therapy, the damage to normal tissues in tumor thermotherapy can be reduced, and significant application prospects in the field of biomedicine are achieved.

The invention belongs to the field of chemistry and relates to an organic near-infrared two-photon fluorescent dye with a structure in a formula I in the specification. The organic near-infrared two-photon fluorescent dye has the beneficial effects that after being excited, the organic near-infrared two-photon fluorescent dye simultaneously shows stronger luminous efficiency of single-photon near-infrared fluorescence and two-photon fluorescence; the efficiency of two-photon fluorescence is obviously improved compared with that of other carbocyanine dyes; living cell fluorescence microscope experiments show that probes prepared from the fluorescent dye can enter tumor cell lysosome through endocytosis and can be imaged and simultaneously monitored by single-photon fluorescence and two-photon fluorescence microscopes; the dye can achieve synchronous single-photon near-infrared fluorescence and two-photon fluorescence imaging in the in-vivo and in-vitro states; and the fluorescent dye has the characteristics o good water solubility and stable chemical properties, can be used or bio-macromolecular markers and in the field of medical image diagnosis and especially has strong application value in optical image guided tumor excision.

The present invention provides a tumor-targeted nuclear magnetic resonance/fluorescence dual-mode imaging contrast agent, preparation and applications thereof. According to the present invention, the contrast agent is nanometer micro-particles formed by self-assembling a nuclear magnetic resonance amphiphilic molecule, a near-infrared fluorescent dye amphiphilic molecule and a tumor targeting amphiphilic molecule in a solution, can stably exists under a physiological pH value condition, can be specifically accumulated at a tumor site in a targeting manner, can significantly enhance the intensity of the MRI signal and the near-infrared fluorescence signal at a tumor lesion site, can improve the specificity and the sensitivity of nuclear magnetic resonance imaging and near infrared fluorescence imaging scanning, and is expected to be adopted as the contrast agent for fluorescence guidance during tumor resection surgeries and postoperative nuclear magnetic resonance monitoring.

The invention discloses a nano probe material for imaging, which is capable of efficiently breaching blood-brain barriers. Firstly, a high temperature pyrolysis method is adopted for preparing inner core NaYF4:Yb/TM/Gd hydrophobic nano-particles, then an NaGdF4 shell is formed through epitaxial growth so as to form core/casing structured NaYF4: Yb/Tm/Gd and NaGdF4 nano-particles, then hydrochloric acid hydrophilic modification and sulfhydryl PEG modification are performed, and finally, the nano probe material is prepared through Angiopep-2 grafting, and is marked as ANG/PEG-UCNPs. The material can be used for efficient blood-brain barrier breaching, magnetic resonance imaging diagnosis and positioning before an cranial cavity glioma operation, near infrared fluorescence imaging in the operation and image mediating, the imaging effect is good, the sensitivity is high, the physiological system toxicity is low, and the material plays an important significance in successful clinic glioma removing operations and the development and the application of medical image diagnostic techniques.

A hydroxy apatite marked by the nanoparticles of near-infrared fluorescent quantum point for researching the growth of bone and diagnosing and treating the diseases, such as coronary sclerosis, bone cancer, etc is prepared by linking the nanoparticles of near-infrared fluorescent quantum point with the hydroxy opatite via adsorption, ion exchange, or covalent bonding.

The invention relates to a preparation method and applications of a photo-thermal and photodynamic co-used antitumor drug delivery system taking gold nano star mediated by folic acid as the carrier. In the prior art, the antitumor drugs have adverse side and toxic effects, moreover, the biocompatibility and targeting performance of the antitumor drugs are bad, and the provided system solves the problems mentioned above. In the preparation method, reaction active amino and carboxylic groups are grafted onto the surface of gold nano stars through covalent bonds, then targeting molecules are connected to the gold nano stars through amidation reactions to obtain a targeting gold nano star solution; then the amino groups on the end of the targeting compounds carry out chemical reactions with near-infrared fluorescence probes so as to obtain a gold nano star solution with functions of folic acid targeting and near-infrared fluorescence imaging; and finally an antitumor drug (adriamycin) is directly loaded on the surfaces of gold nano stars through an electrostatic adsorption effect. The chemical stability and physical stability of the drug delivery system are both very good, the preparation technology is simple, the resources of the raw materials are abundant, the cost is low, the side and toxic effect is little, and the tumor cell proliferation can be effectively inhibited.

The invention relates to a near-infrared fluorescence imaging surgery guide device and an application thereof. The device comprises a near-infrared laser light source, a light collection optical system, a visual light filter, a near-infrared light filter, a color video camera, a near-infrared video camera and a terminal display. The device can conveniently perform position guide and morphic display functions in the process of a lymph excision or inactivation surgery, and meanwhile can be used for analyzing and evaluating the effect of the lymph excision or inactivation surgery. The device is favorable to improving surgery efficiency and accuracy and accumulating surgery treatment experience.

The invention discloses preparation of near-infrared fluorescence nanoparticles and an application method of the near-infrared fluorescence nanoparticles in an imaging technology. A loaded near-infrared fluorescent dye is taken as a luminescence center, and chitosan and polylysine are taken as basic skeletons, so as to prepare the near-infrared fluorescence nanoparticles by self-assembling package of sodium alginate into a shell. A transmission electron microscope displays the mean grain sizes of the near-infrared fluorescence nanoparticles are about 15nm; a dynamic scattering test indicates that the mean dynamic hydration radius is less than 160nm; the photostability of the near-infrared fluorescence nanoparticles is significantly enhanced in comparison with fluorescent dye molecules; the near-infrared fluorescence nanoparticles can stably exist in 0-1.5% NaCl aqueous solution.

The invention belongs to the technical field of organic chemistry and discloses a conjugated chain functional benzoindole hematocyanine dye and an application. The stability of the novel hematocyaninedye is obviously superior to that of an existing cyanine fluorescent probe, and the novel hematocyanine dye has the multiple functional activity such as near infrared fluorescence and tumor mitochondria targeted accumulation and photo-thermal and photo-power synergic treatment of killing tumor cells. Applied as the near infrared fluorescence probe in living cell imaging research and living body infrared optical molecular imaging, a precise and efficient photo-thermal and photo-power antitumor effect is achieved synchronously. The dye can be applied to near infrared in vivo optical imaging andphoto-sound imaging of tumors, is applied to optical assisted operation navigation, and has an efficient antitumor function. The dye not only can develop a target tissue in real time, but also kill tumor cells specifically. The preparation method of the benzoindole hematocyanine dye is mild in reaction condition, simple in step and easy to operate and low in raw material cost, and is easy to purify and relatively high in yield.