65results about How to "Achieve markup" patented technology

The invention discloses a fluorescent probe for detecting pH value and a preparation method thereof, and a special detection kit. The structure of the fluorescent probe for detecting pH value is disclosed as Formula I. The invention also discloses a preparation method of the compound disclosed as Formula I, which comprises the following steps: (1) carrying out reduction reaction on dihydroxybenzaldehyde disclosed as Formula IV and morpholine to obtain substituted morpholinyl methoxyphenyl diol disclosed as Formula V; and (2) in an inert atmosphere, carrying out substitution decomposition rearrangement reaction on the substituted morpholinyl methoxyphenyl diol disclosed as Formula V and tricarbocyanine dye disclosed as Formula VI in the presence of alkali to obtain the compound disclosed as Formula I. The kit for detecting pH value comprises the compound disclosed as Formula I and a solvent. The fluorescent probe and special detection kit thereof have favorable responsivity for pH value of solutions, can implement marking of lysosome and detection of pH value of the cytolysosome, is simple to operate, has the advantages of low cost, high speed, high sensitivity and the like, and can easily implement popularization and application.

The invention belongs to the technical field of biological detection, and particularly relates to an up-conversion luminescence nano material based on triplet state-triplet state annihilation, a preparation method thereof and an application thereof in biological imaging. The invention provides an up-conversion luminescence material based on triplet state-triplet state annihilation, which can be applied to biological imaging. The up-conversion luminescence material based on triplet state-triplet state annihilation for biological imaging comprises a sensitizer and an annihilation agent, wherein the sensitizer is classified into three types; the first type of sensitizer is a porphyrin and phthalocyanine metal complex, and the metals in the complex include palladium, platinum, iridium and zinc; the second type of sensitizer is an iodo- or bromo-boron fluorine compound; and the third type of sensitizer is a complex of ruthenium, iridium and platinum. According to the material provided by the invention, the annihilation agent is a biphenyl and acene compound and a derivative thereof, or a bromo-boron fluorine compound or an imine luminous compound. The material has the advantages of high sensitivity, photobleaching resistance and the like, and has great application values in terms of biological marking and imaging.

The invention relates to a micro-nanofluidic chip for achieving rapid fluorescent labeling of proteins, which is designed according to different sizes of the proteins and a fluorescent probe. A nano-structure is led in a micro-nanofluidic system to construct a micro-nanofluidic chip system. The proteins are gathered through a nano channel, and the labeled proteins are gathered through a micro-channel. The invention further provides a method for achieving teh rapid fluorescent labeling of the proteins by using the micro-nanofluidic chip. The micro-nanofluidic chip and the method for achieving the rapid fluorescent labeling of proteins can achieve the rapid fluorescent labeling of the proteins, are particularly suitable for microminiaturization labeling of microgram-level and nanogram-level proteins, nucleic acid and other precious bio-molecules, do not need additional purification processes and chromatographic separation to remove excess unreacted dye molecules, and are simple and convenient to operate.

The invention relates to an application of nanoparticles with fluoride lutecium as a main component in biological detection and preparation of a reagent for biological detection. Furthermore, the invention also relates to the nanoparticles used in the above application and a preparation method thereof. The dimension of the material is less than 200nm. The material has an excellent luminescence property and can be used in bioimaging and biodetection as a luminescent probe.

The invention discloses a method for calculating a ring content of IPRAN network access layer equipment, which comprises the following steps of: 1, acquiring IPRAN network resource data, wherein the acquired IPRAN network resource data comprises IPRAN network element information, PRAN relay information and tunnel information; 2, according to an equipment type and network structure information, carrying out layering on the acquired IPRAN network equipment; 3, according to the acquired network element information and relay information, carrying out network connection on a network element, and generating a topological graph of a local IPRAN network; 4, firstly, defining equipment ring formation, and then calculating the ring content of the access layer equipment by the following steps; and 5, automatically carrying out statistics to generate related reports of IPRAN network evaluation. According to the method disclosed by the invention, automatic acquisition of IPRAN resources, automatic generation of a topology and automatic calculation of a link attribute of the network equipment are implemented, and by automatic linkage of the IPRAN equipment and base station equipment, evaluation and analysis of a base station protection attribute can be further implemented.

Embodiments of the invention disclose a data monitoring processing method and apparatus. The method comprises the steps of performing total monitoring on a current system to obtain corresponding current total log information, and monitoring an I / O operation event in the current system to obtain corresponding current I / O operation log information, wherein the current total log information is stored in a first memory, and the current I / O operation log information is stored in a second memory; performing comparative analysis on the current I / O operation log information and multiple pieces of historical I / O operation log information in the second memory to determine an operation type corresponding to the current I / O operation log information; and if the operation type corresponding to the current I / O operation log information is an operation type required to be optimized, transferring and storing all the total log information in the first memory into an ETL file, and performing optimization marking in the ETL file according to a comparative analysis result. With the adoption of the method and apparatus, the manpower cost can be reduced and the analytic efficiency and accuracy of I / O operation event information are improved.

The invention relates to a preparation method and application of an environment estrogen sensor marked by nitrogen-doped graphene and belongs to the technical field of novel functional materials and biological sensing detection. The sensor takes an Au@Ag heterogeneous alloy as a substrate material and the nitrogen-doped graphene as a detection antibody decorative material; and by utilizing a layer-by-layer nano self-assembling technology, a sandwiched immunosensor is constructed. By using the synergistic catalysis effect of the Au@Ag heterogeneous alloy and the nitrogen-doped graphene, the sensitivity of the environment estrogen sensor is remarkably improved and the linear range is expanded, so that the preparation method and application of the environment estrogen sensor marked by the nitrogen-doped graphene have very important meanings on detection and analysis of environment estrogens.

The invention relates to a method for marking enveloped virus nucleocapsid by using a quantum dot. The method is characterized in that a capsid protein sequence blended with a biotin receptor peptide sequence and a catalytic receptor peptide biotinylation enzyme sequence are simultaneously inserted in a virus whole-genome so as to obtain a recombinant virus plasmid, the biotinylation of the nucleocapsid is realized by virtue of the reproduction process of the virus plasmid in a host cell, and then the quantum dot coupled with streptavidin is used for realizing the marking of the enveloped virus nucleocapsid. The method does not needs the violent chemical reaction transformation and the marking of a virus and is beneficial to the maintenance of infection activity of the virus self; and the marking of the nucleocapsid in the enveloped virus does not affect the surface of the enveloped virus and the indentation of the surface. The method can be applied to the virus infection mechanism study and the virus-related disease treatment research.

The invention relates to an encoding detection method based on polymer microsphere change. The encoding detection method comprises the following steps of: identifying antibody, antigen, DNA or RNA (Ribonucleic Acid) segments in samples to be detected through immunization or gene hybridization specificity by adopting a series of polymer microspheres of the antibody, the antigen, the DNA or the RNA segments with surface coupling specificity, wherein the polymer microspheres have different grain diameters and internal structures; carrying out specific coupling on a molecular probe with the surface carrying fluorescent substances and the antibody, the antigen, the DNA or the RNA segments; detecting fluorescence signals through a detection device, comparing the samples with negative and positive control samples or the samples with known concentration so as to judge whether the samples to be detected are negative or positive; characterizing the grain diameters and the internal structures of the microspheres in the detection device, and accurately judging the substances to be detected according to the corresponding relation between the grain diameters and the internal structures of the microspheres as well as the detection information. The invention is based on immunization and gene hybridization specificity, inaugurates an encoding technology, can realize the detection on a plurality of samples or indexes to be detected simultaneously, and is simple, fast and reliable.

The invention discloses a detection method of a porous silicon photonic crystal biochip. The detection method comprises the following steps: 1, depositing Si3N4 on P-type monocrystalline silicon through a plasma enhanced chemical vapor deposition technology, and preparing a 8*8 micro-array device by using a standard photoetching technology; 2, electrochemically corroding the micro-array device in dark and normal temperature environment, wherein each of circular units in the micro-array device is a porous silicon micro-cavity; 3, connecting the porous micro-cavities to the detection optical path of the optical characteristics of the surface of the micro-array device; and 4, measuring the reflectivity of the surface of the micro-array device by using the detection optical path, converting the change of the reflectivity into the brightness change of a corresponding area in a digital image, analyzing the gray scale grade of the digital image corresponding to the brightness change of the corresponding area, and detecting the refractive index with high sensitivity.

The invention relates to an automatic collecting and marking device for cage-rearing meat duck breeding information and belongs to the technical field of livestock breeding. The automatic collecting and marking device comprises a duck frame cage, an automatic control system, guide rail and traveling systems, an automatic feeding system, a feed intake measurement mechanism, an egg weight weighing and identifying mechanism, a duck weight weighing device and a spraying device, wherein the spraying device is positioned above the duck frame cage, and the duck weight weighing device is connected with the duck frame cage. The automatic collecting and marking device has the advantages that production performance parameters such as meat duck feed intake, laying time and egg quality can be monitored and subjected to code spraying in real time, and meat duck weight weighing and timely spraying can be achieved; the automatic collecting and marking device is of special practical significance to recording various parameters involving line breeding, feed, nutrition and the like of poultry precisely, stably and continuously and reducing workload.

The invention relates to a travel product operation log recording method and system. The log recording method comprises the steps that when interaction between a client and a server starts, a corresponding tracking ID is created; when the client or the server completes data processing, log information of data processing of the client or the server and the tracking ID are correspondingly stored; and when the client sends data to the server or the server sends data to the client, the tracking ID is set in the data transmission mode of the client and the server. According to the travel product operation log recording method and system, the tracking ID is created when interaction is initiated between the client and the server, the process of interaction between the client and the server is tracked by means of the tracking ID, the tracking ID and the log information of interaction are correspondingly stored, therefore, marking and storing of the log information of interaction are achieved,the coupling among the log information is improved, and the log information is conveniently extracted.

The invention belongs to the technical field of nondestructive testing of marked neutron beams, in particular to a movable D-T neutron generator for the nondestructive testing of marked neutron beams.The movable D-T neutron generator comprises a high-frequency ion source system (5) and a vacuum system and measuring unit (7) which are connected through an accelerating tube (6), and an associated beam target system (8) arranged on the vacuum system and measurement unit (7). The D+ ion beams generated and led out by the high-frequency ion source system (5) bombard a tritium target (68) in the associated beam target system (8) after being accelerated by an accelerating tube (6), the 14MeV neutrons are generated through D-T reaction, and the associated beam target system (8) measures the generation time and position of the neutrons at the same time, so that the calibration of the emitting time and direction of the neutrons is achieved. The movable D-T neutron generator also comprises a control system used for controlling the high-frequency ion source system (5) and the accelerating tube (6). The present invention is the portable D-T neutron generator that can be adapted for the photography application of the associated beam neutrons.

The invention discloses an amide-substituted azaindole-pentamethine cyanine dye as well as a synthesis method and application thereof, and mainly relates to synthesis of the amide-substituted azaindole-pentamethine cyanine dye and application of the amide-substituted azaindole-pentamethine cyanine dye in the aspects of biological recognition imaging, nucleic acid labeling, DNA sequencing and the like. The dye has an active group and a water-soluble group for further amidation reaction. The dye has near-infrared absorption and high fluorescence quantum yield, can be used for biological recognition and detection of amino compounds, and is applied to the fields of protein labeling, nucleic acid detection, DNA sequencing and the like.

The invention relates to a single-cage pure-line laying hen breeding information acquisition and marking automation system, belonging to the technical field of livestock breeding. The single-cage pure-line laying hen breeding information acquisition and marking automation system comprises a chicken frame cage body, an automatic control system, a guiderail and walking system, an automatic charging system, a food consumption measurement mechanism, as well as an egg weighing and marking mechanism, wherein the egg weighing and marking mechanism and is positioned on the lower part of the guiderail and walking system, an egg outlet guide plate is lapped together with the egg weighing and marking mechanism, and the egg weighing and marking mechanism and an egg collection device are connected; an automatic manure cleaning mechanism is arranged at the bottom of the chicken frame cage body. The single-cage pure-line laying hen breeding information acquisition and marking automation system realizes real-time monitoring and marking on production performance parameters such as laying hen food consumption, egg laying time and egg quality of laying hens. The single-cage pure-line laying hen breeding information acquisition and marking automation system has special practical significance on precisely, stably and continuously recording all parameters in the problems of livestock strain breeding, feed, nutrients and other problems, and lightening the workload.

The invention discloses a secure data transmission and control system based on an SDN (Software Defined Network) multi-domain network, which comprises a user registration and login unit, a data collection unit, a data recognition unit, a data processing unit, a data distribution unit and a data storage unit. According to the method, the to-be-transmitted data uploaded by the user and the recognition data are integrated to obtain the transmission data, so that the data is marked, and later tracing processing on the data is facilitated; by setting transmission priorities, performing different types of queue calibration and performing classified transmission on transmission data according to user requirements, the transmission data can be transmitted according to a certain priority logic, sothat the transmission efficiency can be effectively improved, the data security can be improved. Meanwhile, bad data can be recognized and processed, the network environment can be effectively purified, and the user experience is improved. And the use experience of network users is improved.

The invention discloses a chip system integration method and device and a computer readable storage medium. The method comprises the steps that design information of a plurality of chip sub-modules and a configuration file corresponding to a target chip are obtained, the configuration file at least comprises a preset label used for storing the design information, and the chip sub-modules are used for integrating the target chip; according to the incidence relation between the preset labels and the design information, the design information is stored in the corresponding preset labels, and target labels are obtained; an abstract syntax tree is generated based on the target labels, the abstract syntax tree is composed of a plurality of child nodes, and each child node corresponds to one target label; and according to a connection relationship among the child nodes in the abstract syntax tree, integrating node information of the plurality of child nodes to obtain a target chip system. The technical problem that in the prior art, the integration efficiency is low when an integrated chip system is manually configured is solved.

The invention discloses an intelligent accounting file storage system based on radio frequency identification. The system comprises a cabinet and a tag reader. The cabinet body is divided into three boxes with relatively fixed spaces by longitudinal brackets and transverse partition plates; the three boxes include the closed file box, the financial information box and the seal storage box, and electronic tags are bonded to the surfaces of the boxes respectively; each electronic tag includes a chip, antennas and a substrate, and the antennas and the chips are fixed to the substrates through organic adhesive polymers; each antenna is composed of a rectangular part, a bent part and a chip connecting part, and the rectangular parts and the corresponding bent parts are symmetrically distributedon the left and right sides with the corresponding chip connecting parts as the centers. A special antenna circuit is designed, the performance of the electronic tags is improved, and the system is suitable for various accounting file management application occasions; the electronic tags and the tag reader are utilized to achieve automatic scanning identification, proofreading and marking of accounting files, the intelligent operation is fast and convenient, the precision is greatly improved, and the workload of financial staff is significantly reduced.

The invention discloses a clamping adjustment device of a circuit board in the technical field of circuit board processing. The clamping adjustment device includes an electric push rod, An upper partof the outer wall of the push rod in the electric push rod is provided with a hinge, the left wall of the hinge is clamped with a rotating shaft, the right end of the rotating shaft is connected withthe output shaft of the rotating motor through a coupling, the top wall of the hinge is welded with a mounting plate; Left and right front parts of top wall,left and right parts of that bottom wall and left and right out walls of the mounting plate are provided with slide groove, An L-shape support rod is uniformly clamped from left to right in that inn cavity of the front slide groove of the topwall of the mounting plate, A laser lamp device is sleeved on that out wall of the top of the L-shaped support rod, A sleeve is uniformly clamped to the inner cavity of the left and right outer wall chute of the mounting plate from front to back, a telescopic rod is clamped to the inner cavity of the sleeve, a clamping device is screwed to one end of the telescopic rod, a limiting rod is welded tothe top wall and the bottom wall of the sleeve, and screws are fixedly installed at both ends of the limit rod. The device has the advantages of simple operation, convenient use and wide applicationrange.

The invention discloses a label for an oil sample test tube, and relates to the technical field of electric test appliances. The label for the oil sample test tube comprises a label section, a clamping slot section and a binding band section which are connected in sequence, wherein the label section is a label for identifying the oil sample number, and the clamping slot section and the binding band section form a binding tape. According to the label for the oil sample test tube, marking on the oil sample test tube is realized through the label section, the clamping slot section, the binding band section and the like.

The invention discloses a metal magnetic wire mark for a broad tonguefish, wherein the metal magnetic wire mark belongs to the technical field of an artificial mark. The metal magnetic wire mark comprises a mark post, and a mark cap which is connected with the mark post. The front end of the mark post is a threaded head. The threaded head is connected with a fixing ring. The front end of the mark post is further provided with an RF chip. An electronic chip is embedded in the mark cap. The surfaces of the mark post and the mark cap are provided with rubber layers. The surface of the mark cap is provided with a character mark and a two-dimensional code. The mark post penetrates through a tissue below the dorsal fin of the broad sole through a marking machine or manually. The mark cap contacts with the surface of the broad tonguefish. The fixing ring is connected with the threaded head at the front end of the mark post, thereby finishing fixation of the metal magnetic wire mark and the broad tonguefish. When the broad tonguefish is captured, biological information acquisition can be performed. The acquired biological information can be input into the electronic chip for performing biological information comparing and research when the broad tonguefish is captured at the next time. Furthermore related data of a tag in the RF chip can be read by means of a card reader.

The invention relates to design and synthesis of subcellular area targeting, long-wavelength and dual-wavelength ratio method Ca<2+> fluorescent probe. In the synthesis of the probes, 1, 2-di(2-aminophenoxy)ethane-N, N, N', N'-tetraacetic acid tetraethyl ester is taken as the initial material to first synthesize Ca<2+> receptor (5, 5'-dicarboxo-BAPTA) with two formyl active groups as the key intermediate of the Ca<2+> fluorescent probes. Then, the key intermediate is condensed with the substrate containing active methyl in agreeable organic solvent to obtain a series of Ca<2+> fluorescent probes excited by visible light, fluorescence excitation and emission peak as well as position all change before and after the probes combine with Ca<2+>, and Ca<2+> can be measured by dual-wavelength ratio method. The probes can carry out targeting marking on the specified subcellular area after entering cells. With the probes adopted, the real-time dynamic measurement of Ca<2+> content in the cells and space-time distribution condition can be realized in the subcellular level, thereby, an efficient means for cell calcium signal transduction research is provided.

The invention provides an enterprise performance automatic evaluation method based on ExcelVBA technology. The method comprises the following steps: establishing a performance evaluation index system; reading the original data stored in the plurality of Excel documents into a performance evaluation index system Excel document through a data reading module; processing the value of each index by adopting a min-max standardization method through a data processing module, and carrying out information aggregation through an evidence reasoning method to obtain the value of the total index, namely an enterprise performance evaluation result; through a data checking and marking module, checking the read and calculated data and marking abnormal values to prompt a user to pay attention; and displaying an evaluation result and a month-by-month / quarter / year change trend in the form of a broken line graph in the total index and the shell page of each index through a graphic expression and visualization module.

The invention relates to the technical field of solid waste treatment. and especially relates to a The rural garbage collection and transportation management system which comprises a data acquisitionsubsystem and a positioning analysis subsystem, and the data acquisition subsystem is used for acquiring current positioning information of a vehicle in real time and acquiring previous positioning information and station information of each garbage station at the same time; the positioning analysis subsystem is used for comparing the current positioning information with the previous positioninginformation, ; the method comprises the steps of acquiring current positioning information and station information of a vehicle, marking the state of the vehicle according to a comparison result, generating a station distance according to the current positioning information and the station information when the state is marked as stay, screening out the station distance with the minimum value, comparing the station distance with a sign-in threshold value, and marking a garbage station corresponding to the station distance when the screened station distance is smaller than the sign-in thresholdvalue. By adopting the scheme, the driving condition of the vehicle is supervised, so that the clearing and transporting condition of the garbage station is effectively supervised, and the technicalproblem that the clearing and transporting condition of the garbage station is insufficiently supervised in the prior art is solved.

The invention provides a dynamic detection-based marking device for sponge city engineering, and relates to the technical field of detection marking devices, wherein the dynamic detection-based marking device comprises a main body seat, a first marking part, a cleaning part and a second marking part; the main body seat is mounted on a workbench through bolts, and two baffles are symmetrically welded on the main body seat; plates are placed on the main body seat in a linear array shape; two rollers are in contact with the top end face of the plate, and a sliding rod A, a mounting seat and the rollers jointly form a plate compaction structure, so that the phenomenon of detection result error caused by non-compaction of the plates can be prevented; and the problems that in an existing device, when the thickness of the plates is too high, the plates are blocked by an object similar to a blocking rod, the detection is realized, but the detection method is relatively low in efficiency, and unqualified plates are inevitably blocked when the plates are pushed to move forwards, so that the unqualified plates need to be taken out at the moment, and the rear plates can be continuously detected are solved.

The invention relates to the technical field of teaching appliances, in particular to a magnetic type circle-drawing precision instrument. The magnetic type circle-drawing precision instrument comprises a center body and a radius measuring scale. The center body is provided with a plane capable of being in touch with a plate body, a cavity provide with a magnetic body is arranged on the center body, a circle center positioning mechanism is arranged at the center position of the center body, the circle center positioning mechanism is used for arranging a pen for drawing a circle center, a scaleplate with a 360-degree angle is fixed to the center body, the radius measuring scale freely rotates around the center line of the center body, and a connecting unit provided with a circle-drawing pen for drawing an arc line is arranged at one end of the radius measuring scale. The magnetic type circle-drawing precision instrument is absorbed on a blackboard by using magnetism, the circle centerposition is marked, circles with any accurate radius length can be drawn through scales on the radius measuring scale, arcs with any angle can be measured and cut by the radius measuring scale by combining with angle indexes, the operation is convenient, and the accurate and distinct circles can be rapidly drawn.

The invention discloses an electrode for micro-administration in vivo and conducting electrophysiological recording. The electrode includes an administration electrode, a recording electrode and a recording electrode sealer, a glass microelectrode is arranged on the lower segment of the administration electrode, the top end of the glass microelectrode is connected to the lower end of a silicone sleeve by a lower fixing ring of a metal right-angle adapter, the upper end of the silicone sleeve is connected to an administration soft hose by an upper fixing ring of the metal right-angle adapter, and a gap is formed between the umbrella part of the recording electrode and an opening of the tail end of the glass microelectrode. According to the electrode for micro-administration in vivo and conducting electrophysiological recording, the tip end of the recording electrode penetrates through the opening of the tail end of the glass microelectrode, the tip end of the glass microelectrode has asmall overall diameter, damage to the tissue is small, through micro-injection, precise administration of tiny nuclei can be achieved, the lengths of the glass microelectrode and the recording electrode can be adjusted according to the actual situation during making, and the electrode is suitable for administration and recording of an encephalic region with at different depths.

The invention belongs to the technical field of marking neutron beam non-destructive testing, in particular to a mobile D-T neutron generator for marking neutron beam non-destructive testing, comprising a high-frequency ion source system ( 5) and the vacuum system and the measurement unit (7), the associated beam target system (8) arranged on the vacuum system and the measurement unit (7), the D+ ion beam generated and drawn by the high-frequency ion source system (5) passes through the accelerated The tube (6) accelerates and bombards the tritium target (68) in the associated beam target system (8), and produces 14 MeV neutrons through the D-T reaction, and the associated beam target system (8) simultaneously monitors the generation time and position of the neutrons. The measurement realizes the calibration of the emission time and direction of the neutrons; it also includes a control system for controlling the high-frequency ion source system (5) and the accelerating tube (6). The present invention is a portable D-T neutron generator applicable to correlative beam neutronography applications.

The invention discloses a method for calculating a ring content of IPRAN network access layer equipment, which comprises the following steps of: 1, acquiring IPRAN network resource data, wherein the acquired IPRAN network resource data comprises IPRAN network element information, PRAN relay information and tunnel information; 2, according to an equipment type and network structure information, carrying out layering on the acquired IPRAN network equipment; 3, according to the acquired network element information and relay information, carrying out network connection on a network element, and generating a topological graph of a local IPRAN network; 4, firstly, defining equipment ring formation, and then calculating the ring content of the access layer equipment by the following steps; and 5, automatically carrying out statistics to generate related reports of IPRAN network evaluation. According to the method disclosed by the invention, automatic acquisition of IPRAN resources, automatic generation of a topology and automatic calculation of a link attribute of the network equipment are implemented, and by automatic linkage of the IPRAN equipment and base station equipment, evaluation and analysis of a base station protection attribute can be further implemented.

The invention relates to a preparation method and application of an environment estrogen sensor marked by nitrogen-doped graphene and belongs to the technical field of novel functional materials and biological sensing detection. The sensor takes an Au@Ag heterogeneous alloy as a substrate material and the nitrogen-doped graphene as a detection antibody decorative material; and by utilizing a layer-by-layer nano self-assembling technology, a sandwiched immunosensor is constructed. By using the synergistic catalysis effect of the Au@Ag heterogeneous alloy and the nitrogen-doped graphene, the sensitivity of the environment estrogen sensor is remarkably improved and the linear range is expanded, so that the preparation method and application of the environment estrogen sensor marked by the nitrogen-doped graphene have very important meanings on detection and analysis of environment estrogens.