Flow cytometry

A flow cytometer and cell technology, applied in the field of flow cytometer, can solve the problems of high price of liquid control system, low quantum efficiency, reverse bias, etc., and achieve good photon counting performance, good time resolution, instrument Small and compact effect

Inactive Publication Date: 2011-06-08
SUZHOU INST OF BIOMEDICAL ENG & TECH
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  • Description
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  • Application Information

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Problems solved by technology

[0003] At present, commercial flow cytometers use gas lasers, solid-state lasers, etc., which have the disadvantages of large volume, complex structure, and high maintenance costs. Some flow cytometers use a single excitation light source, which limits the selection of cellular fluorescent dyes.
The detectors of flow cytometers generally use photomultiplier tube

Method used

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Embodiment Construction

[0020] see figure 1 As shown, a flow cytometer includes a light source irradiation system 15, a liquid flow system 16, a spectroscopic system 11, a signal detection analysis processing system 14 and a PC display control system 13, and the liquid flow system 16 makes the cell sample in the sheath Flow in a straight line under the wrapping of the flow, the light source irradiation system 15 performs laser irradiation on the cell flow of interest marked by the fluorescent dye in the liquid flow system 16, and excites to obtain the labeled fluorescent emission signal, and passes through the spectroscopic system 11 Enter the signal detection analysis processing system 14 for signal detection, analysis and output, the PC display control system 13 displays and stores the information of the signal detection analysis processing system 14 and feedback controls the light source irradiation system 15 , a liquid flow system 16 and a signal detection analysis processing system 14, the liqui...

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Abstract

The invention discloses a flow cytometry. The flow cytometry comprises a light source irradiation system, a liquid flow system, a light split system, a signal detection, analysis and processing system, and a personal computer (PC) display control system; the liquid flow system comprises a flowing chamber; linearly flowing cells are accommodated in the flowing chamber; a solid laser is used as an excitation light source of the light source irradiation system, and laser passes through a reflecting mirror and a plano-convex lens to be converged and irradiated on the flowing chamber, is integrated and then is further converged on the optical part of the flowing chamber to be irradiated on the linearly flowing cells; the signal detection, analysis and processing system comprises a lateral scattering detector, a yellow fluorescence channel detector, a near-infrared fluorescence channel detector, a forward scattering detector and a signal processing module; and all detectors are multi-pixel photon counters (MPPC). The flow cytometry has the characteristics of large dynamic detection range, high sensitivity, small size and compactness and convenience in maintenance.

Description

technical field [0001] The invention belongs to a biomedical detection instrument, in particular to a flow cytometer. Background technique [0002] Flow cytometer (FCM) is an advanced scientific and technological equipment integrating optics, electronics, fluid mechanics, cytochemistry, biology, immunology, laser and computer and other disciplines and technologies. It is widely used in clinical medicine, cytology, biology, microbiology, pharmacy, reproductive science and other fields. It is one of the advanced instruments in modern scientific research and is known as the "CT" of the laboratory. It performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis or sorting technology on single cells or biological particles in a fast linear flow state. It has fast detection speed, multiple measurement parameters, large amount of collected data, comprehensive analysis, and sorting. High purity, flexible method and so on. [0003] At present, commercial flow c...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 武晓东陈永勤王策赵书涛
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH
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