Flow cytometry

Abstract

The invention discloses a flow cytometry. The flow cytometry comprises a light source irradiation system, a liquid flow system, a light split system, a signal detection, analysis and processing system, and a personal computer (PC) display control system; the liquid flow system comprises a flowing chamber; linearly flowing cells are accommodated in the flowing chamber; a solid laser is used as an excitation light source of the light source irradiation system, and laser passes through a reflecting mirror and a plano-convex lens to be converged and irradiated on the flowing chamber, is integrated and then is further converged on the optical part of the flowing chamber to be irradiated on the linearly flowing cells; the signal detection, analysis and processing system comprises a lateral scattering detector, a yellow fluorescence channel detector, a near-infrared fluorescence channel detector, a forward scattering detector and a signal processing module; and all detectors are multi-pixel photon counters (MPPC). The flow cytometry has the characteristics of large dynamic detection range, high sensitivity, small size and compactness and convenience in maintenance.

Description

technical field [0001] The invention belongs to a biomedical detection instrument, in particular to a flow cytometer. Background technique [0002] Flow cytometer (FCM) is an advanced scientific and technological equipment integrating optics, electronics, fluid mechanics, cytochemistry, biology, immunology, laser and computer and other disciplines and technologies. It is widely used in clinical medicine, cytology, biology, microbiology, pharmacy, reproductive science and other fields. It is one of the advanced instruments in modern scientific research and is known as the "CT" of the laboratory. It performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis or sorting technology on single cells or biological particles in a fast linear flow state. It has fast detection speed, multiple measurement parameters, large amount of collected data, comprehensive analysis, and sorting. High purity, flexible method and so on. [0003] At present, commercial flow c...

AI Technical Summary

Problems solved by technology

[0003] At present, commercial flow cytometers use gas lasers, solid-state lasers, etc., which have the disadvantages of large volume, complex structure, and high maintenance costs. Some flow cytometers use a single excitation light source, which limits the selection of cellular fluorescent dyes.

The detectors of flow cytometers generally use photomultiplier tubes (PMT), which have the advantages of large photosensitive area and low noise equivalent power (NEP), but they are bulky, have low quantum efficiency, and high reverse bias voltage. Works in the UV and visible spectral range, weak resistance to external magnetic fields

[0004] Liquid control systems for commercial flow cytometers are expensive, bulky and complex

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