The invention discloses a near-
infrared two-region dual-channel fluorescent
nanoprobe as well as a preparation method and application thereof, and relates to the technical field of biomedical imaging. A nano probe A and a nano probe B included in the near-
infrared two-region dual-channel fluorescent nano probe are both of a core-shell structure, two NIR-IIb fluorescent nano probes adaptive to different excitation wavelengths (808 nm and 980 nm) are constructed by precisely regulating and controlling the types and proportions of
lanthanide elements, dual-channel imaging is achieved, limitation of a
single excitation wavelength is broken through, spectral
crosstalk is eliminated, and the near-
infrared two-region dual-channel fluorescent nano probe has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method has the characteristics of material uniformity and
biological safety. The near-infrared IIb region dual-channel imaging
system has the characteristics of high temporal-spatial resolution and
signal separation, and has significant application value in the field of
living body multi-target synchronous observation. Compared with a traditional two-
photon microscope technology, the imaging method has a wider observation view field, a local
craniotomy operation does not need to be carried out on an experimental mouse, and physiological trauma is remarkably reduced.