Single-excitation double-emission carbon-based biomass dot as well as preparation method and application thereof

Abstract

The invention provides a single-excitation double-emission carbon-based biomass dot as well as a preparation method and application thereof. The preparation method comprise S1, a proper number of clean Chinese cabbage leaves are weighted, and are ground in a utensil; S2, after absolute ethyl alcohol and an acetone solution according to the volume ratio of 1:0.9 to 1:1.1 are added, and then the mixture is uniformly mixed for being soaked; S3, after being soaked for 28-32 minutes, filtering is conducted to obtain a chlorophyll extracting solution; S4, 25 mL of chlorophyll extracting solution proportional to 0.1g-0.11g of polyoxyethylene diamine are added in a polytetrafluoroethylene reaction kettle, and mixing and heating are conducted for 9-11h under the temperature controlled to be 195-205DEG C; S5, after cooling to the room temperature is conducted, suction filtration is conducted, and a filtrate is adjusted to that with a pH value roundly equal to 7; and S6, the filtrate is transferred into a dialysis bag for lucifugal dialysis for 11-13h to obtain the single-excitation double-emission carbon-based biomass dot. According to the dot and the preparation method and application thereof, the carbon-based biomass dot can be directly used as a probe for ratiometric fluorescence imaging of active substance coenzyme A in cells, the background fluorescence interference of biological tissue can be eliminated, and the accuracy and the sensitivity of the fluorescence imaging of the coenzyme A can be improved.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to the detection technology of coenzyme A, in particular to a single-excitation double-emission carbon-based biomass quantum dot and its preparation method and application. Background technique [0002] Coenzyme A (coenzyme A, CoA) is an important coenzyme in organisms and a ubiquitous and essential cofactor. It has received extensive attention for its role in neurodegeneration, protein acetylation, cell autophagy and signal transduction. CoA directly or indirectly participates in the promotion of more than 100 chemical reactions in cells, and plays an important role in the metabolism of sugar, fat and amino acids. It has been found that the lack of CoA in the body will affect the catabolism of fatty acids and the clearance of triacylglycerol, thus speculating that this may be one of the causes of type IIb and type IV hyperlipidemia. Therefore, the determination of the...

AI Technical Summary

Problems solved by technology

However, the CDs reported so far are all CDs with single emission and emission wavelength less than 600nm. These CDs cannot be directly used as probes for ratiometric fluorescence imaging of active substances in cells.

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