Preparation method and application of a yellow fluorescent bioimaging material

A biological imaging and yellow fluorescence technology, which is applied to luminescent materials, material analysis through optical means, and analysis of materials, can solve the problems of poor repetition rate and complex synthesis operation methods, and achieve high fluorescence efficiency and good photobleaching resistance. Effect

Active Publication Date: 2021-12-10
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing yellow fluorescent bio-imaging material synthesis operation method is complicated and the repetition rate is poor
The synthesis method is simple and easy to operate, and the synthesis method of yellow fluorescent bioimaging materials with good repeatability has not been reported

Method used

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  • Preparation method and application of a yellow fluorescent bioimaging material
  • Preparation method and application of a yellow fluorescent bioimaging material
  • Preparation method and application of a yellow fluorescent bioimaging material

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Experimental program
Comparison scheme
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Embodiment 1

[0060] A schematic embodiment of the present invention, a method of preparing a yellow fluorescent biomaraminated material, comprising the steps of:

[0061] Step S1: Preparation of yellow fluorescence biomara

[0062] Step S11: Weighing 4 g of phenanthemum, slowly adding the phenanthemone into the concentrated nitric acid, stirred while stirring, after refluxing at a temperature of 80 ° C, cooling;

[0063] Step S12: The solution obtained by preparing to step S11 is added to the excess deionized water, and the filtration washing until pH is neutral, drying to obtain 1,3,6-trinitrofrofromin;

[0064] Step S13: 0.1 g of 1,3,6-trinitr-nitroflyrene and sodium sulfite is weighed, and then dissolved in 40 ml of ethanol, then transferred to polytetrafluoroethylene, at 200 ° C for 12h;

[0065] Step S14: The resulting solution was filtered through the 0.22 μm filter, and the unreacted particles were removed, and the crude product was obtained;

[0066] Step S2: The yellow fluorescent bio...

Embodiment 2

[0074] Another embodiment of the present invention, a method of preparing a yellow fluorescent biomaraminated material, comprising the steps of:

[0075] Step S1: Preparation of yellow fluorescence biomara

[0076] Step S11: 2 g of a pyrene, slowly add the phenanthemone in a 150 ml of concentrated nitric acid, stir, while stirring, after 24 h at a certain 60 ° C temperature, cool;

[0077] Step S12: The solution obtained by preparing to step S11 is added to the excess deionized water, and the filtration washing until pH is neutral, drying to obtain 1,3,6-trinitrofrofromin;

[0078] Step S13: Weighing 1,3,6-trinitr-nitroflyfoxy and sodium sulfite each, 0.05 g of sulfite, dissolved in 20 ml of ethanol, then transferred to polytetrafluoroethylene, at 180 ° C for 6 h;

[0079] Step S14: Filtering the solution obtained by step S13 using a 0.20 μm filter film, removes unreacted particles to obtain a crude product;

[0080] Step S2: The yellow fluorescent biological imaging material prep...

Embodiment 3

[0088] Another embodiment of the present invention, a method of preparing a yellow fluorescent biomaraminated material, comprising the steps of:

[0089] Step S1: Preparation of yellow fluorescence biomara

[0090] Step S11: Weigh 6 g of pyrene, slowly add phenanthemone to 450 ml of concentrated nitric acid, stir the binding and stir, after reflowing at a temperature of 100 ° C, cooling;

[0091] Step S12: The solution obtained by preparing to step S11 is added to the excess deionized water, and the filtration washing until pH is neutral, drying to obtain 1,3,6-trinitrofrofromin;

[0092] Step S13: 0.2 g of 1,3,6-trinitr-nitroxyl and sodium sulfite were weighed, and 60 ml of ethanol was dissolved, and then transferred to polytetrafluoroethylene, reacted at 220 ° C for 18 h;

[0093] Step S14: The resulting solution was filtered through the 0.24 μm filter, and the unreacted particles were removed, and the crude product was obtained;

[0094] Step S2: The yellow fluorescent biologic...

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Abstract

The invention relates to a method for preparing a yellow fluorescent bioimaging material, comprising: step S1: preparing the yellow fluorescent bioimaging material; step S2: performing column chromatography purification on the yellow fluorescent bioimaging material prepared in step S1. It also relates to a yellow fluorescent bioimaging material. The invention also relates to the application of a yellow fluorescent bioimaging material. The advantage is that the yellow fluorescent bioimaging material is prepared by using the solvothermal method with small chemical molecules as precursors; the yellow fluorescent bioimaging material has the characteristics of high fluorescence efficiency and good photobleaching resistance; the material can be used as a biological probe label Reagents for cell and in vivo imaging.

Description

Technical field [0001] The present invention relates to the field of biomaramount materials, and more particularly to a method of preparing a yellow fluorescent biomaraminated material and its application thereof. Background technique [0002] Conventional biomaramics include traditional quantum points, AIE materials, and fluorescent polymers, but contain some of the inherent defects such as toxicity, biocompatibility difference, and loss of light emission. The graphene quantum dots have been known as the best instead of replacing traditional materials with their good biocompatibility and low toxicity. It has been widely used. [0003] The graphene quantum point is a zero-dimensional graphene nano material, which is generally less than 100 nm and the number of layers can be divided into one layer, 2 layers, and 3-10 layers. The size of the graphene quantum point and some surface structures determine the size of the gap size of the graphite alone, generally between 2-7 eV. The gra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C303/32C07C309/43C09K11/06G01N21/64A61K49/00
CPCC07C303/32C09K11/06G01N21/6428A61K49/0021C09K2211/1011C07C309/43
Inventor 沈龙祥潘登余
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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