Fluorescent nano-particle with composite silica shell structure and preparation thereof

A fluorescent nano-composite technology, applied in the field of fluorescent nanoparticles and its preparation, can solve the problems of low fluorescence efficiency of fluorescent nanoparticles, and achieve the effects of improved detection sensitivity, high fluorescence efficiency, and less dye leakage

Inactive Publication Date: 2009-05-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the shortcomings of low fluorescence efficiency of the existing preparation of fluorescent nanoparticles, the purpose of the present invention is to provide a nano-gold / rhodamine dye / silicon dioxide with high fluorescence efficiency, controllable particle size, simple process and good particle stability Fluorescent Nanoparticles with Composite Silicon Shell Structure

Method used

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  • Fluorescent nano-particle with composite silica shell structure and preparation thereof
  • Fluorescent nano-particle with composite silica shell structure and preparation thereof
  • Fluorescent nano-particle with composite silica shell structure and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1: Preparation of fluorescent nanoparticles with nano-gold / rhodamine B / silica composite silicon shell structure

[0036] Adopting chloroauric acid-sodium citrate reduction method to prepare particle diameter is the nano-gold solution of 25 ± 5nm; Adding the rhodamine B solution (5.0 × 10 -6 mol / L), and the reaction continued for 6 hours to obtain a nano-gold solution coated with rhodamine B dye, which was called the reaction solution. The pH value of the reaction solution is adjusted between 10-11 by ammonia water. Under magnetic stirring, add an ethanol solution of ethyl orthosilicate (1.0×10 -3 mol / L), and react for 12 hours under magnetic stirring to obtain fluorescent nanoparticles with nano-gold / rhodamine B / silica composite silicon shell structure with a particle size of 50-60 nm.

Embodiment 2

[0037] Example 2: Preparation of fluorescent nanoparticles with nano-gold / rhodamine B / silica composite silicon shell structure (fluorescent nanoparticles with larger particle size)

[0038] Adopting chloroauric acid-sodium citrate reduction method to prepare particle diameter is the nano-gold solution of 25 ± 5nm; Adding the rhodamine B solution (1.0 * 10 -5 mol / L), and the reaction continued for 12 hours to obtain a nano-gold solution coated with rhodamine B dye, which was called the reaction solution. The pH value of the reaction solution is adjusted between 10-11 by ammonia water. Under magnetic stirring, add the ethanol solution (5.0 × 10 -3 mol / L), react under magnetic stirring for 24 hours to obtain fluorescent nanoparticles with nano-gold / rhodamine B / silica composite silicon shell structure with a particle size of 60-70 nm.

Embodiment 3

[0039] Example 3: Preparation of fluorescent nanoparticles with nano-gold / rhodamine 6G / silica composite silicon shell structure

[0040] Adopting chloroauric acid-sodium citrate reduction method to prepare particle diameter is the nano-gold solution of 25 ± 5nm; Adding the rhodamine 6G solution (1.0 * 10 -5 mol / L), and the reaction continued for 6 hours to obtain a nano-gold solution coated with rhodamine 6G dye, which was called the reaction solution. The pH value of the reaction solution is adjusted between 10-11 by ammonia water. Under magnetic stirring, add the concentration of 1.0×10 -3 mol / L ethyl orthosilicate ethanol solution reacts for 24 hours under magnetic stirring to obtain fluorescent nanoparticles with nano-gold / rhodamine 6G / silica composite silicon shell structure with a particle size of 50-60 nm.

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Abstract

The invention discloses a fluorescent nanometer particle with a composite-type silicon shell structure and a preparation method thereof. The shell composition of the fluorescent nanometer particle is silicon dioxide; and the fluorescent kernel is nanometer gold particle of which the surface is combined with rhodamine dye. The method for preparing the fluorescent nanometer particle comprises the following steps: the surface of the nanometer gold particle is wrapped with fluorescent dye and is used as a fluorescent kernel material; and a silicon oxide shell is formed on the surface of the fluorescent kernel through ethyl orthosilicate. The nanometer particle has the advantages of high fluorescent efficiency, simple preparation process, good biocompatibility and the like.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to a fluorescent nanoparticle with a nano-gold / rhodamine dye composite fluorescent inner core and a silicon dioxide outer shell and a preparation method thereof. Background technique [0002] Rhodamine dyes (such as rhodamine B and rhodamine 6G) have strong fluorescence and are widely used in various bioanalysis techniques, including quantitative determination of biological macromolecules, fluorescence immunoassay detection and targeted fluorescence imaging. [0003] At present, nanoparticles are widely used in various fields of biomedicine because of their special optical and electrical properties. The silica shell is directly wrapped on the surface of the dye to develop fluorescent nanoparticles with a silica shell structure, which can avoid direct contact between the dye and the studied organism and reduce the biological toxicity. Existing fluorescent nanoparticles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06
Inventor 蔡怀鸿蔡继业杨培慧梁旭竞韦炜
Owner JINAN UNIVERSITY
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