598 results about "Fluorescent stain" patented technology

The invention provides an analysis method of multiple immunotissue fluorescent staining images and related equipment, and is applied to the technical field of data processing. The method comprises the steps of obtaining original biological sample data; slicing the original biological sample data to generate a plurality of pieces of initial biological slice information; processing the initial biological section information to generate a fluorescent staining section image; processing the fluorescent staining slice image to generate an aligned fluorescent staining slice image; performing fusion processing on the aligned fluorescent staining slice images to generate a target fluorescent staining image; processing the target fluorescent dyeing image to generate initial region information; the initial area information is processed, images of a plurality of different fluorescence channels are generated, and the images of the plurality of different fluorescence channels are single fluorescence channel images; and processing the images of the plurality of different fluorescence channels to generate cell clustering information.

The invention relates to a detection kit for epithelial-mesenchymal transition in EB virus infected thymoma and application, the detection kit comprises a quantum dot labeled antibody E-Cadherin and a quantum dot labeled antibody Vimentin, a quantum dot is coupled with a specific antibody, a thymoma paraffin section sample is dyed through the quantum dot and the specific antibody conjugate, and the EB virus infected thymoma is obtained. The conjugate can be specifically connected with an EMT related antigen expressed in the slice, so that the quantum dots can be used for qualitative or quantitative detection of the EMT-generated thymoma cells. The quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of epithelial cells and mesenchymal tissues in the thymoma microenvironment is achieved, number detection and position detection can be achieved, and EMT condition evaluation is facilitated.

A vascular access device (VAD) monitoring system includes a VAD with a catheter tube that is subcutaneously disposed and incorporates a fluorescent dye. An optical imaging system is also provided, comprising a light source emitting infrared (IR) excitation light and a camera capable of detecting signal light emitted by the fluorescent dye upon exposure to the excitation light. This enables real-time monitoring of the VAD, allowing for the detection of any potential complications or issues related to the vascular access device. The combination of the catheter tube with the fluorescent dye and the optical imaging system provides a non-invasive and efficient means of monitoring the VAD, enhancing patient safety and improving overall healthcare outcomes.

The invention belongs to the technical field of medicine, and discloses an active targeting atherosclerotic plaque liposome and application thereof in diagnosis, a carrier material for forming the liposome comprises targeting peptide R8 modified methoxy polyethylene glycol phospholipid (R8-DSPE-PEG), polypropylene sulfide-polyethylene glycol (PPS-PEG) and cholesterol (CH), and the targeting peptide R8 modified methoxy polyethylene glycol phospholipid (R8-DSPE-PEG), the polypropylene sulfide-polyethylene glycol (PPS-PEG) and the cholesterol (CH) are combined to form the active targeting atherosclerotic plaque liposome. The sequence of the targeting peptide R8 is Asp-Ser-Ser-Ser-Gln-Cys-His-Arg, and the sequence of the targeting peptide R8 is Asp-Ser-Ser-Gln-Cys-His- The liposome provided by the invention can improve the enrichment concentration of a fluorescent dye or a drug in local lesion, and has the advantages of low toxicity and high efficiency, and the obtained targeted liposome has a good effect on diagnosis of atherosclerosis.

The invention relates to the technical field of fluorescent dyes, in particular to a citric acid-based fluorescent dye for distinguishing dead cells from living cells as well as a preparation method and application of the citric acid-based fluorescent dye. The invention discloses a citric acid-based fluorescent dye for distinguishing dead cells from living cells. The citric acid-based fluorescent dye comprises at least one of a compound 1 and a compound 2, the citric acid-based fluorescent dye can specifically enter dead cells and specifically dye cytoplasm of the dead cells, so that the dead cells can be dyed or imaged. The citric acid-based fluorescent dye is difficult to dye living cells, so that dead cells and living cells can be quickly and accurately distinguished, and the citric acid-based fluorescent dye can be applied to the field of biological medicines.

The invention relates to a method for detecting Breg cells and Tfh cells in a chest adenoma tissue microenvironment, which comprises the following steps of: coupling quantum dots with a specific antibody, dyeing a chest adenoma tissue slice sample, and specifically connecting the specific antibody with antigens on the Breg cells and / or Tfh cells in the slice sample cells, so as to quantify or position the Breg cells and / or Tfh cells by the quantum dots. According to the method disclosed by the invention, the existing multicolor immunofluorescence technology is improved, and a secondary antibody dyeing link is omitted by using a method of carrying the antibody by the quantum dots, so that the efficiency is improved; the quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of Breg cells and Tfh cells in a thymoma tumor microenvironment is achieved, and biological behaviors of the Breg cells and the Tfh cells can be determined conveniently; the immune condition can be evaluated by detecting the ratio condition of Breg / Tfh cells in the breast adenoma tumor microenvironment of a patient in vitro.

The invention relates to the technical field of immunochromatography, in particular to a silicon-coated color fluorescent microsphere and a mild preparation method and application thereof.The preparation method comprises the steps that firstly, time-resolved fluorescent dye is synthesized in an ethanol system, then oil-soluble dye, an ethanol water solution and a swelling agent dichloromethane are added, stirring is conducted at the room temperature to enable the dye to be dissolved, and then the silicon-coated color fluorescent microsphere is obtained; and adding ammonia water and a silane coupling agent tetraethyl orthosilicate, reacting overnight, supplementing the ammonia water, gradually adding a coupling agent 3-aminopropyltriethoxysilane, and washing with pure water for several times after the reaction is finished. The particle size range of the prepared color time-resolved fluorescent microspheres is 200-450 nm, the color time-resolved fluorescent microspheres are prepared in a green and mild mode through a one-step method, a surface active agent, a dispersing agent and a surface amino modified silicon dioxide matrix are not used, the high-luminous-intensity binuclear rare earth europium complex and the oil-soluble dye are embedded into the microspheres in the polymerization process, the dye is not prone to leakage, and the color time-resolved fluorescent microspheres are environmentally friendly and environmentally friendly. The method can be used for qualitative and quantitative dual immunodetection in the field of immunochromatography.

The invention provides a detection method and application of miRNA and analogues thereof, and relates to the field of nucleic acid detection. According to the invention, a probe 1 and a probe 2 are designed for target miRNA and miRNA analogues; the probes are respectively combined with a part of miRNA, the probe 2 can be combined to a chip, a microsphere and other solid phase carriers, and the probe 1 modifies a fluorescent dye or biotin and other signal labeling materials; after the target miRNA or miRNA analogue is mixed with the probe 1 and the probe 2 and incubated, the probe 2 is not hybridized with the miRNA analogue and only hybridized with the miRNA analogue and captures the target miRNA by optimizing the hybridization temperature, so that the detection specificity is high.

A measurement spectrum obtained by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light is separated for each fluorescent dye with high accuracy. Provided is a particle analysis system (1) including a plurality of photodetectors (23) configured to acquire light generated by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light; and an information processing unit (132) configured to calculate fluorescence intensity of each fluorescent dye by performing separation processing on a measurement spectrum based on measured values from the plurality of photodetectors (23) with a single staining spectrum of each fluorescent dye, in which the separation processing is performed by using a weighted least squares method (WLSM) including a weight determined based on a variation in the measured values.

To provide an indwelling clip that allows easy visual recognition of light emission of a fluorescent body from the outside of a luminal organ and that enables excellent mounting stability to the interior wall of the luminal organ.SOLUTION: An indwelling clip 1 has: a pair of arm plate parts 22 that are made of metal plates and opened elastically into a substantial V-shape; and claw parts 23 that are formed at the respective front ends of the arm plate parts 22. The arm plate parts are each provided, at least on an outer surface, with a fluorescent body that includes a fluorescent pigment emitting red or near infrared light by application of excitation light, the fluorescent body being an extrusion molding or an injection molding of a polymeric material composition containing the fluorescent pigment.SELECTED DRAWING: Figure 1

The present invention belongs to the field of medical technology, and discloses a liposome that actively targets atherosclerotic plaques and its application in diagnosis. The carrier material forming the liposome includes a targeting peptide R8 modified with methoxy polyethylene glycol phospholipid (R8-DSPE-PEG), polysulfide propylene-polyethylene glycol (PPS-PEG) and cholesterol (CH), and the sequence of the targeting peptide R8 is Asp-Ser-Ser-Ser-Gln-Cys-His-Arg. The liposome of the present invention can increase the enrichment concentration of fluorescent dyes or drugs in the local lesion, has the advantages of low toxicity and high efficiency, and the obtained targeted liposome has a good effect on the diagnosis of atherosclerosis.

The invention relates to the technical field of valve tests, in particular to a valve test bench which comprises a support, a liquid storage tank is arranged on the support, a suction pipe is arranged in the liquid storage tank in a communicating mode, a bottom plate is fixedly connected to the outer side wall of the support, an observation opening is formed in the bottom plate, an electromagnetic valve is arranged in the observation opening, and a valve is arranged in the electromagnetic valve. An observation opening is formed in the support, a valve is arranged on the observation opening, a hand wheel is arranged on the side wall of the valve, electric rods are arranged on the two sides of the support, clamping plates are arranged on the electric rods and make contact with the outer wall of the valve, and a detection mechanism is arranged on the support and comprises a hydraulic rod fixedly connected to the support. Through the arrangement of mechanisms such as the inner box, after the fluorescent dye introduced into the valve is detected, the inner box can spray clean water into the valve in a self-adaptive mode at the moment, the fluorescent dye in the valve is washed, and the situation that the fluorescent dye is stored in the detected valve is prevented.

The invention relates to a lung cancer cell drug resistance classification method and system based on high content imaging. The method comprises the following steps: carrying out fluorescent staining and multi-component labeling on cells to be detected; performing high-content imaging on the to-be-detected cells subjected to fluorescent staining to obtain a multi-channel cell image; carrying out drug resistance classification on the multi-channel cell image by utilizing an IRSNet network to obtain a classification result; wherein the IRSNet classification network comprises an Inception-v4 backbone network, an Inception-ResNet module, a Reduction module and a softmax function, and the Inception-v4 backbone network, the Inception-ResNet module, the Reduction module and the softmax function are connected in sequence. The Inception-v4 backbone network is used for extracting the image features of the multi-channel cell image; the Inception-ResNet module is used for improving the depth and the width of the Inception-v4 backbone network and introducing a residual error structure; the Reduction module is used for reducing the network structure of the image features; the softmax function is used for classifying the image features. Compared with the prior art, the method is higher in cell drug resistance classification precision and efficiency.

The invention provides a prostate lesion tissue immunofluorescence detection kit and a detection method, and belongs to the technical field of kits. The kit is prepared from the following raw materials in parts by weight: 40 to 60 parts of TYR-xxxPlus fluorescent dye, 30 to 45 parts of TSA Buffer, 20 to 40 parts of Polymer-HRP monoclonal antibody mouse / rabbit second antibody, 15 to 30 parts of antibody, 5 to 15 parts of Dapi dye liquor, 50 to 70 parts of antibody diluent and 50 to 70 parts of peroxidase confining liquid. According to the kit, a non-biotin amplification system is adopted, so that compared with a traditional double-staining experiment, the experiment time is greatly shortened, and interference of endogenous biotin is effectively avoided. The method is suitable for diagnosis and differential diagnosis of prostate cancer, PIN and AAH of prostate resection specimens, acupuncture biopsy specimens, electric resection specimens and transurethral resection specimens, and is particularly suitable for conventional formalin fixed paraffin embedded sections.

The invention discloses a synthetic method of a fluorescent dye, which comprises the following steps: A, dropwise adding POCl3 into DMF (Dimethyl Formamide) at the temperature of 0 DEG C under the condition of protective atmosphere and stirring, and fully reacting; continuously dropwise adding cyclohexanone, heating, stirring, fully reacting, adding the reaction liquid into ice water, and separating out a solid first compound; b, under the protective atmosphere, stirring and refluxing p-sulfonyl phenylhydrazine, 3-methyl-2-butanone and acetate in acetic acid to react completely, cooling the reaction liquid to-10 to 5 DEG C, stirring, and separating to obtain a second compound; c, under the protective atmosphere, after the second compound and 1, 4-butanesultone are stirred, refluxed and completely reacted in xylene, separating to obtain a third compound; and D, stirring and refluxing the third compound, the first compound and acetate in methanol at the temperature of 80 DEG C under the conditions of protective atmosphere and light shielding, simultaneously dropwise adding acetic anhydride, cooling to room temperature after full reaction, and separating and purifying. According to the scheme, the synthesis process is simple and efficient, and high-quality synthesis of the target dye S0456 is effectively realized.

The invention discloses an assay system with multiple detection modes and a related method. The measurement system with multiple detection modes comprises a sample stage; the optical module is used for scanning a sample to be detected and at least has a sequencing mode and a fluorescent staining mode; a driving circuit selectively coupled to mode parameters corresponding to an operation mode of the optical module, the mode parameters including at least a first mode control parameter corresponding to the sequencing mode and a second mode control parameter corresponding to the fluorescent staining mode; the mode switching circuit is coupled to the driving circuit so as to control the driving circuit based on the sample to be detected; and the mode switching circuit associates different mode parameters to the driving circuit based on the type of the sample to be detected. According to the invention, multi-mode switching of the detection system is realized, and multiple detection functions are realized on the same platform.

The invention discloses an automatic sample processing chip and system for milk somatic cell analysis. The automatic sample processing chip comprises a filtering channel, a mixing channel, a quantitative channel, a sample solution inlet hole, a fluorescent dye inlet hole and a detection liquid outlet hole; the filtering channel is used for filtering a sample solution, the mixing channel is used for dyeing the filtered sample solution, and the quantitative channel is used for quantitatively outputting the dyed sample solution; an inlet of the filtering channel is communicated with the sample solution inlet hole, an outlet of the filtering channel is communicated with an inlet of the mixing channel after being converged with the fluorescent dye inlet hole, an outlet of the mixing channel is communicated with an inlet of the quantitative channel, and an outlet of the quantitative channel is communicated with the detection liquid outlet hole. The automatic sample processing chip has the advantages of being low in cost, small in size, easy to clean and the like, the demanded quantity of samples and reagents and the detection cost are greatly reduced, the automatic sample processing system can achieve continuous and automatic sample injection of the samples, and the sample processing speed is remarkably increased.

The invention discloses a multiplex fluorescence immunohistochemical staining kit and application thereof. The multiplex fluorescence immunohistochemical staining kit comprises a plurality of casein amide substrate fluorescent dyes; the multi-tyramine substrate fluorescent dye comprises an aggregation-induced emission tyramine substrate and at least one narrow-spectrum tyramine substrate, the Stokes shift of the aggregation-induced emission tyramine substrate is larger than or equal to 150 nm, and the Stokes shift of the narrow-spectrum tyramine substrate is smaller than or equal to 60 nm. The full width at half maximum of the emission spectrum of the narrow-spectrum caseinate substrates is less than or equal to 50nm, and if the number of the narrow-spectrum caseinate substrates is more than or equal to two, the difference value of the maximum excitation wavelengths of any two narrow-spectrum caseinate substrates is more than or equal to 30nm. According to the present invention, the aggregation-induced emission tyrosine amide substrate with the large Stokes shift is used for the multiple fluorescence immunohistochemical staining, such that the staining multiple number is effectively improved, and the spectrum cross color does not occur;

The invention discloses lithium battery packaging leakproofness detection equipment and a detection method thereof, and relates to the technical field of lithium battery packaging leakproofness detection.The lithium battery packaging leakproofness detection equipment comprises an operation table, a battery shell and a gas inlet device used for inputting detection gas; the piston plate is arranged in the fixed cylinder in a sliding manner and is driven by air pressure; the adjusting unit is in driven connection with the piston plate and is used for simulating the sealing damage state of the battery shell; the limiting unit is in driven connection with the piston plate and is used for being matched with the adjusting unit to change the sealing state of the battery shell; the damage state of the sealing gasket is simulated through the interaction of the upper magnet and the lower magnet, the sealing performance of the lithium battery shell used for a period of time is detected, whether the dye leaks or not is observed through the cooperation of the transparent marking frame arranged on the battery shell and the fluorescent dye, and therefore the leakproofness of the battery shell is detected.

The invention provides a loop-mediated isothermal amplification primer composition for combined detection of multiple equine animal viruses, a disposable centrifugal micro-fluidic chip, an LAMP micro-fluidic chip kit and a nucleic acid detection method of the LAMP micro-fluidic chip kit. The loop-mediated isothermal amplification primer composition comprises nucleotide sequences as shown in SEQ ID: 1 to SEQ ID: 16. A fluorescent dye and a loop-mediated isothermal amplification primer composition are pre-embedded in the disposable centrifugal micro-fluidic chip. The LAMP micro-fluidic chip kit comprises a disposable centrifugal micro-fluidic chip and an LAMP amplification working solution. The problem of multiple detection of VSV, NiV, EEEV and WEEV in equine animal viruses is solved, and the detection method is high in specificity, short in reaction time, safe, simple and convenient to operate, high in sensitivity, low in cost and suitable for being used when a temporary virus detection laboratory is established.

The invention discloses an SYBR Green I fluorescent dye quantitative PCR (polymerase chain reaction) method for identifying clostridium butyricum, and belongs to the technical field of molecular detection. The invention provides a primer pair for qPCR (quantitative polymerase chain reaction) identification of clostridium butyricum. The primer pair comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO. 1; 1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO. 2. The identification sensitivity is high, the detection limit is 7.2 * 10 < 2 > CFU / mL, the specificity is strong, and the repeatability is good. Meanwhile, the bacterial genome is obtained by cracking the bacterial liquid with the crude extraction cracking liquid, so that the genome obtaining process is simplified. According to the method, the concentration of the clostridium butyricum liquid in the sample is directly obtained through standard curve conversion, the problems that a clostridium butyricum identification method is complex, long in consumed time, low in efficiency and the like are effectively solved, and a more convenient identification method is provided for related research of clostridium butyricum.

The invention relates to a kit for detecting plasma cells and related antibody levels in a thymus microenvironment and application of the kit. The kit comprises a quantum dot labeled antibody CD138, a quantum dot labeled antibody CD21, a quantum dot labeled AchR, Musk and / or LRP4 receptor, an antigen repair solution and a DNA fluorescent dye solution, wherein the quantum dot labeled antibody CD138, the quantum dot labeled antibody CD21, the quantum dot labeled AchR, the quantum dot labeled Musk and / or the quantum dot labeled LRP4 receptor, the antigen repair solution and the DNA fluorescent dye solution; through the kit, one or more of plasma cells, follicular DC and related antibodies in a thymus (tumor) sample can be detected, so that the immune level of a tissue slice sample is obtained. A method of carrying the antibody by using the quantum dots is adopted, so that a secondary antibody dyeing link in the existing multicolor immunofluorescence technology is omitted, and the efficiency is improved; the quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of plasma cells and follicular DCs in the thymoma tumor microenvironment is achieved, and both quantity detection and position detection can be achieved.

The invention provides an aza [3.2. 1] bridged ring substituted fluorescent dye as well as a preparation method and application of the aza [3.2. 1] bridged ring substituted fluorescent dye. According to the fluorescent dye, an aza [3.2. 1] bridge ring is adopted as a substituent group of the fluorescent dye, so that the brightness and the light stability of the fluorescent dye are remarkably improved, the light blue shift phenomenon generated after the dye is illuminated for a long time is thoroughly avoided, meanwhile, the water solubility of the fluorescent dye can be effectively improved, and good cell membrane permeability of the fluorescent dye can be kept. The invention also provides an embodiment of the functionalized derivative of the dye for single-molecule imaging, confocal imaging and super-resolution imaging in a biological sample.

Sulfonated 2(7)-aminoacridone and 1-aminopyrene dyes and their use as fluorescent tags, in particular for carbohydrate analysis The invention relates to fluorescent dyes with multiple negatively charged groups in their ionized form which are aminoacridone sulfonamides or 1-aminopyrenes having of one of the following general formulae A-D: Formula (A), Formula (B), Formula (C), Formula (D), wherein the ionizable groups X are typically selected from the following: SH, COOH, SO3H, OSO3H, OP(O)(OH)2, OP(O)(OH)Ra, P(O)(OH)2, P(O)(OH)Ra, where Ra═C1-C4alkyl or substituted C1-C4alkyl. The invention further relates to the use of these dyes as fluorescent tags, in particular for reducing sugars and glycans.

The invention belongs to the technical field of targeting peptides, and particularly relates to an EGFR targeting peptide, a fluorescent probe, a composition and application. The targeting peptide has an amino acid sequence as shown in SEQ ID NO: 1. Through verification, the targeting peptide has the capability of selective enrichment in tumor cells, and is beneficial to tumor recognition and treatment. The fluorescent probe coupled by the targeting peptide and the ICG fluorescent dye can specifically recognize and target an EGFR receptor, realizes effective aggregation and retention at a tumor part, is suitable for targeted diagnosis and treatment of tumors, can realize accurate imaging, has important clinical transformation potential, and becomes a molecular imaging probe with application prospects.

The invention belongs to the technical field of photosensitizer preparation and biological medicine, and particularly relates to a photosensitizer and a preparation method and application thereof. The I-type photosensitizer Nile violet NV is designed and synthesized on the basis of fluorescent dye Nile red through monatomic substitution and structural modification, and the structural formula of the I-type photosensitizer Nile violet NV is shown in the formula I. The photosensitizer based on Nile red can generate O2 <.-> after illumination, and has I-type photodynamic therapy potential; the nanomaterial has a good active oxygen generation capability in tumor cells, and is beneficial to the application of tumor photodynamic therapy. The invention proves that the compound shows an excellent anti-tumor effect in mouse solid tumors and can be widely applied to the field of anti-tumor treatment; meanwhile, the I-type photosensitizer is simple in synthesis method, good in biological safety and remarkable in tumor photodynamic therapy effect, so that the I-type photosensitizer has good practical application value.

The invention discloses an afterglow-color-adjustable circularly-polarized room-temperature phosphorescent polymer film and a preparation method and application thereof, and the afterglow-color-adjustable circularly-polarized room-temperature phosphorescent polymer film is prepared by taking a chiral naphthalene phosphate derivative as an energy and chiral transfer donor and selecting a fluorescent dye rhodamine B (RB) as an acceptor. And co-doping with a polymer matrix to obtain the afterglow color adjustable circular polarization room-temperature phosphorescent polymer film. The obtained polymer film has good transparency, high room-temperature phosphorescence efficiency and asymmetric factors, accurately controllable multi-color afterglow and ultra-long afterglow life, which means that the polymer film has important application prospects in the fields of multiple anti-counterfeiting encryption and the like.

The invention relates to a fluorescent probe for measuring the aperture of an ultrafiltration membrane as well as a preparation method and application of the fluorescent probe. The fluorescent probe is a group of fluorescently-labeled proteins obtained by mixing a group of fluorescent dyes which have groups capable of reacting with amino groups in proteins and have non-interfering fluorescence signals with a group of proteins which have significant difference in equivalent diameter and can keep stable in a detection solution for reaction, and then performing purification and concentration. When the fluorescent probe is used as a reference substance to measure the aperture of the ultrafiltration membrane, the fluorescent probe is prepared into a mixed solution, then a filter membrane to be detected is used for filtering the mixed solution at one time, the fluorescence intensity is detected, and the rejection rate R of each fluorescent probe is further calculated. And obtaining apertures d50 and d90 according to a fitting curve of the equivalent diameter and the retention rate R, and drawing an aperture distribution diagram. The method can accurately measure the aperture of the ultrafiltration membrane of 2-20 nm, is convenient to operate, high in efficiency and low in test cost and complexity, and has important significance in research and application in the fields of materials, biology, environment and the like.

The invention relates to a bronchoalveolar lavage fluid rapid detection method based on a fluorescent staining technology and a corresponding detection device, and aims to improve the detection efficiency of alveolar lavage and sampling screening. The detection device comprises an endoscope assembly, a lavage fluid supply module, a recovery module and a control module. Wherein the recovery module is internally provided with an assembly used for adding a fluorescent coloring agent into the recovery liquid, the fluorescent coloring agent is injected into the recovery liquid in the recovery process of the lavage fluid according to needs, and an electromagnetic drive stirring mechanism is combined, so that dynamic and uniform mixing of the dyeing liquid and the lavage fluid is achieved. The control module automatically adjusts the injection amount, the mixing speed and the stirring duration of the coloring agent according to preset parameters or real-time detection data, and the dyeing reaction time in the operation process is remarkably saved.

The invention relates to the technical field of cell statistics, and provides a sperm cell classification statistical method based on artificial intelligence and related equipment. The method comprises the following steps: acquiring a fluorescent staining image of a target sperm cell population, and performing gray level conversion on the fluorescent staining image to obtain a gray level image; performing optimization adjustment on the grayscale image to obtain an optimized image, and detecting the optimized image by using the target detection model to obtain a plurality of cell contours; segmenting the optimized image according to all the cell contours to obtain a plurality of sub-images, and denoising all the sub-images to obtain a plurality of target sub-images; and performing color statistics on all cell contours in all target sub-images to obtain the number of DNA fragmented sperm cells of various degrees. According to the method, the cost of sperm cell classification statistics can be reduced, and the accuracy is improved.