750 results about "Fluorescent stain" patented technology

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

Signature protected photosensitive optically variable (POV) inks are provided, which are capable of providing a unique signature in addition to other security features of POV inks. The inks contain at least two types of colorants, and a third, signature component and other ingredients to enable printing. A first colorant comprises a fluorescent dye and/or pigment emitting light within a characteristic emission band when excited by fluorescent-exciting radiation. A second colorant comprises a dye and/or pigment having a light absorption band at overlapping or longer wavelengths than the characteristic emission band of the first colorant in such a way as to result in a dark color. The third component is a fluorescent/phosphorescent rare earth composition. The inks give dark visible ink images, which also produce detectable coincident fluorescent and phosphorescent images. The inks can be used with detectors of red phosphorescence to achieve a new level of security in high speed sorting operations.

The invention discloses a preparation method of waterborne polyurethane containing a fluorescent dye, comprising the following steps: carrying out a reaction between the fluorescent dye containing reactive hydrogen and isocyanate and grafting parent dyes on polyurethane molecular chains, carrying out methods such as chain extension, crosslinking, side suspension of active groups and introduction of a hydrophilc agent, introducing crosslinking points, active groups and hydrophilic groups on the polyurethane chains, making molecular weight large enough, neutralizing and emulsifying to prepare the waterborne polyurethane fluorescent dye. The fluorescent polyurethane prepared by the preparation method still has strong two-photon effect and photoluminescence effect in an aqueous phase and an adhesive film. Thus, the waterborne polyurethane containing the fluorescent dye can be applied in aspects of anti-counterfeiting mark, traffic sign, biological development, biological detection, medicine tracing, chemical detection, fluorescent ink, fluorescent paint and the like.

A method for treating a lesion of an animal, the animal having at least one vessel that carries blood to the lesion, comprising locating the vessel, administering a composition comprising a photodynamic agent, applying energy to the vessel to photocoagulate the vessel and thereby reduce the rate at which the treatment composition exits said lesion and applying energy to said lesion, of a type and an amount sufficient to excite the photodynamic agent, causing the lesion to undergo photodynamic therapy. Preferably, a dye that is both a fluorescent dye and a radiation absorbing dye, such as indocyanine green dye, is added to the treatment composition to allow (a) confirmation of the presence of the treatment composition in the lesion to be detected by fluorescent angiography and (b) the rate of blow flow to be reduced in the blood vessel feeding the lesion using dye enhanced photocoagulation.

A method of high spatial resolution imaging a structure in a sample comprises: marking the structure with molecules of a fluorescent dye; selecting a first wavelength for excitation light which excites the molecules of the fluorescent dye via a multi photon process for spontaneous emission of fluorescent light; focussing pulses of the excitation light into the sample to excite those molecules of the fluorescent dye present in a focal area of the focussed excitation light; selecting a second wavelength shorter than the first wavelength for de-excitation light which de-excites excited molecules of the fluorescent dye prior to their spontaneous emission; during a plurality of the pulses of the excitation light, continuously directing the de-excitation light onto the sample to de-excite excited molecules of the fluorescent dye, which are located outside an measurement area which is a fraction of the focal area; and recording the fluorescent light spontaneously emitted by the molecules of the fluorescent dye in the sample.

The invention discloses a white light organic electroluminescent device, which comprises a light emitting layer. Multiple layers of single-color light emitting layers with complementary colors are stacked and combined to form the light emitting layer, the main material of at least one single-color light emitting layer is a thermal active delay fluorescent material, and the main material of each single-color light emitting layer is doped with a phosphorescent dye or a fluorescent dye. The thermal active delay fluorescent material is introduced to manufacturing of a white light device, the internal quantum efficiency of the material of the type is more than 25%, and the efficiency is high. As no precious metal material is used, the material cost is low.

Disclosed herein are compositions and methods for imaging nerve cells. The composition comprises a fluorescent dye; and a viral component selected from a neurotropic, replication-defective virus, a viral protein of a neurotropic virus, and a capsid of a neurotropic virus. Although the fluorescent dye in itself cannot penetrate nerve cells, the fluorescent dye is bound to the viral component to form a dye/viral component complex that is capable of penetrating nerve cells.

Disclosed are fluorescent dye compounds of Formula (I):

wherein R₁, R₂, and G are as described herein. The fluorescent dyes are suitable for use in luminescent solar collectors or as a colorant. Methods of making the fluorescent dyes and luminescent solar collectors comprising them are also described.

Colloidal silica particles containing a fluorescent dye compound, composed of a silica particle containing a silica component and a fluorescent dye compound chemically bound or adsorbed thereto,

wherein the colloidal silica particles containing a fluorescent dye compound have an average diameter of 30 nm or less, and wherein said silica particles are used simultaneously as fluorescent-labelling nanobeads; a fluorescent nano-material comprising said colloidal silica particles; and a biochip and an assay method using the same.

The invention discloses an organic electroluminescent device. The organic electroluminescent device comprises a light emitting layer and is characterized in that a main body material of the light emitting layer comprises a first main body material and a second main body material, the first main body material and the second main body material are both thermal active delay fluorescent materials, the main body materials are doped with fluorescent dyes, the difference between forbidden bandwidth of the first main body material and the forbidden bandwidth of the second main body material is larger than 0.2eV, and the difference between wavelengths corresponding to peaks is within 50 nanometers after photoluminescence spectrum of the second main body material and absorption spectrums of the fluorescent dyes are highly normalized. More rapid energy transmission to the fluorescent dyes from a singlet state of the light emitting layer of the organic electroluminescent device is achieved, thus, energy transmission to the singlet state from a triplet state of a main body is promoted, high quantum efficiency is acquired, and the efficiency reduction trend is slow with increase of a current.

The invention relates to a heptamethine cyanine active fluorescent probe and a preparation method and application thereof. The structural formula of the heptamethine cyanine active fluorescent probe is as shown in the specification, wherein X=II-IX; each of R1 and R2 is (CH2)mCH3, (CH2)nOH, (CH2CH2O)pCH3 and CH2C6H5; each of R3 and R4 is H, SO3H, SO3Na and SO3K; each of a, b, c, d, e, f and g is 2-8; each of n, m and p is 1-10. The heptamethine cyanine active fluorescent probe has the advantages that the heptamethine cyanine active fluorescent probe is based on near-infrared long-wave heptamethine cyanine dye, indoline is selected as the aroma parent nucleus to increase fluorescence intensity, and methenyl chain intermediate cyclohexene rigid bridging enhances stability; nitrogen derivatives with chemical reactivity sites are used to perform nucleophilic substitution on the meso-position of the heptamethine cyanine parent dye, and accordingly Stokes shift and active chemical groups areincreased greatly to facilitate the fluorescent labeling of various substances; the fluorescent probe is of a symmetrical structure, preparation and purification processes are simplified, and cost islowered favorably; the probe can be used as the fluorescent labeling probe of biological molecules such as high-sensitivity protein, sugar and DNA and nano carriers to perform cell or living-body horizontal fluorescence imaging, and the like.

The invention relates to a method for fluorescent staining of orlon, which belongs to the field of dye synthesis and textile dyeing. Stilbazole salt is taken as a cation fluorescent dye, 0.2 and 2.5 percent owf fluorescent dye, 0.5g/L Peregal 0, a 1.0 percent owf leveling agent and 4g/L glauber salt are mixed to prepare a dye solution, acetic acid and a sodium acetate buffer solution are used to adjust the pH value of the dye solution to 5, and the dyeing processing with the dyeing bath ratio of 100 to 1 is performed to obtain a fluorescent orlon fabric. The stilbazole salt provided by the method has simple synthesis reaction, has relative flexibility in molecular design and assembly, and also has good linear optical performance and non-linear optical performance; and under specific dye bath concentration, the dyed orlon fabric satisfies the coordinate range of orange color stipulated in European EN471 standard High-visibility Warning Clothing for Professional Use-Test Methods (2003).

The invention relates to a reversible solid photochromic fluorescence ink material and application thereof, belonging to the technical field of photochromic materials. The particular molecular structure uses aromatic ring or fragrant heterocyclic ring substituent group and spiropyrane as basic structural units, the structural formula is disclosed in the specification, and the material is novel fluorescent dyes synthesized by esterification reaction. The research detects that the dyes have different spectral absorption and (or) fluorescence dual color variations before and after the ultraviolet irradiation; the absorption and fluorescence color variations caused by the ultraviolet light can reversibly recover under certain temperature heating conditions to the absorption and fluorescence color before the ultraviolet irradiation; and thus, the material has excellent stability, reversibility and fatigue resistance. The ink material can be used independently, or compounded with a polymer, ink or the like to be used as a photochromic material. The ink material is applicable to the fields of safety forgery prevention, safety inks, display devices, fluorescent transduction, information storage and the like.

The invention belongs to the field of high-polymer materials and discloses a high-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as a preparation method and an application of the high-polymer vesicle. The high-polymer vesicle is mainly formed through self-assembly of the AIE molecules and amphiphilic block copolymers; the outer layer and the inner layer of a high-polymer vesicle film are hydrophillic layers, each hydrophillic layer is formed by hydrophilic chain sections in the amphiphilic block copolymers, and a film intermediate layer between the outer layer and the inner layer is formed by hydrophobic chain sections in the amphiphilic block copolymers and the AIE molecules. The high-polymer vesicle has high fluorescence intensity and can meet different fluorescent staining demands. The preparation method is simple and feasible, the production efficiency is high, and the repeatability is good. The high-polymer vesicle can be used for related fields of optical bioimaging, biological detection and clinical imaging, high-polymer vesicle research and the like.

Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit ''single-tube'' processing.

The invention discloses a lysosome-targeted fluorescent dye capable of realizing red emission and near-infrared emission, and a preparation method and application thereof, belonging to the field of bioluminescence analysis. The preparation method comprises the following steps: dissolving a compound with the substituent R in an anhydrous organic solvent and adding morpholinoindolal and a catalyst under the condition of introduction of nitrogen, wherein a mol ratio of morpholinoindolal to the compound with the substituent R is 1-10: 1; and carrying out a reaction at a reaction temperature of 25 to 200 DEG C for 1 to 24 h, concentrating an obtained solution and carrying out silica-gel column chromatography so as to obtain the target fluorescent dye. The fluorescent dye prepared in the invention is applicable to targeted imaging of lysosomes in cells, fluorescent probes or laser dyes. The fluorescent dye has the advantages that the emission wavelength of the fluorescent dye is in a range from red zone to near-infrared zone; the fluorescent dye can prevent interference of biological background fluorescence and has high fluorescence quantum efficiency and good light stability; and the fluorescent dye can be specifically localized in lysosomes, so the fluorescent dye has high application value.

A method for distinguishing erythrocytes in a biological specimen, comprising the steps of preparing a sample liquid by performing to give a damage to a cell membrane of yeast-like fungi without hemolyzing erythrocytes in a biological specimen and to stain the yeast-like fungi with a fluorescent dye; detecting a first information and a second information from a particle in the sample liquid, wherein the first information reflects a size of the particle and the second information reflects a degree of fluorescent staining of the particle; and distinguishing the erythrocytes from the yeast-like fungi based on the first information and second information detected, is disclosed. An apparatus, a reagent kit and a reagent for carrying out the method are also disclosed.