45 results about "Forensic identification" patented technology

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

This invention discloses a wearable intelligent joint range of motion measurement device for forensic clinical identification, belonging to the field of forensic identification equipment technology. The device includes a wearable fixation component, a posture sensing unit, a data processing unit, a storage and interaction unit, and a power supply unit. It synchronously collects limb posture data from both sides of the joint using dual inertial sensors, calculates high-precision joint range of motion, and automatically matches the data using a built-in disability assessment standard library to output a preliminary suggestion for the disability level. This invention offers objective measurement, convenient wear, and traceable data, specifically addressing the problems of large errors, low efficiency, and susceptibility to subjective interference in traditional forensic manual measurements. It is suitable for limb injury disability assessment and possesses strong practicality and professionalism.

This invention discloses a method for predicting sudden cardiac death based on forensic autopsy data using machine learning and its forensic applications, belonging to the fields of machine learning, statistics, and forensic identification. This invention provides a method for screening independent predictors of forensic diagnosis of sudden cardiac death using LASSO regression and logistic regression. Using 10-fold cross-validation, the method selects the minimum lambda(λ) to identify 14 risk factors. Logistic regression ultimately identifies 9 independent predictors, including age, heart weight, left ventricular wall thickness, right ventricular wall thickness, interventricular septum thickness, aortic valve circumference, mitral valve circumference, liver weight, and left kidney weight. A nomogram and a web-based calculator are constructed for predicting sudden cardiac death in forensic practice, thereby determining sudden cardiac death using objective indicators. This method has not been reported worldwide.

The invention relates to the technical field of forensic appraisal, and provides a method and system for constructing a drowning appraisal model based on virtual anatomy, and the method comprises the steps: obtaining image data corresponding to a plurality of corpses obtained through scanning the plurality of corpses based on different CT scanning devices; segmenting each piece of image data by using image processing equipment to obtain a VOI mask file corresponding to each corpse output by the image processing equipment; extracting original image omics characteristics in the VOI mask file; screening specific radiomics characteristics meeting screening conditions in the original radiomics characteristics; and constructing a multi-organ joint identification model based on a training set and a test set generated by the specific radiomics characteristics. According to the scheme, the phenomenon that the identification result is inaccurate due to the influence of external subjective and objective factors can be avoided, and the accuracy of drowning identification is improved.

This invention discloses a semen-specific mRNA-cSNP primer composition, kit, and method based on SNaPshot technology, belonging to the field of molecular identification technology. The primer composition provided by this invention includes amplification primers with nucleotide sequences as shown in SEQ ID NO. 1-34 and single-base extension primers as shown in SEQ ID NO. 35-52. This invention further provides a method for identifying human semen samples, including extracting sample mRNA, reverse transcription to obtain cDNA, performing an amplification reaction using amplification primers, and then performing a single-base extension reaction using single-base extension primers. Experimental results show that the identification method of this invention has advantages such as high specificity, high sensitivity, and good reproducibility, and can accurately identify individual semen donors, which is of great significance for achieving accurate semen-related criminal investigations and forensic identification.

This invention provides a method and system for automatically generating forensic reports, relating to the field of judicial information technology. The method includes: acquiring multimodal forensic materials of the object to be identified; extracting key information tags using a multi-label classification model to generate a tag set; constructing a prompt template for forensic identification based on the tag set using an automatic prompt search algorithm; determining key discovery description paragraphs by combining the prompt template and a language model; performing slot processing on the key discovery description paragraphs and the standard forensic report template using a slot classifier to determine the target position of the template; filling the key discovery description paragraphs into the target position of the template and optimizing the description content in the filled template to generate a draft forensic report; determining whether the draft forensic report passes quality verification; if so, outputting the draft forensic report as the forensic report; otherwise, adjusting the key discovery description paragraphs according to the quality verification results.

This invention discloses a cSNP primer composition, kit, and its application for body fluid tracing based on next-generation sequencing technology, relating to the fields of forensic biological evidence and molecular identification technology. The cSNP primer composition includes primer pairs amplifying 34 cSNP genetic markers. The primer composition for body fluid tracing based on next-generation sequencing technology provided by this invention relates to 34 cSNP markers for identifying five types of body fluids. This invention has developed and established a kit capable of determining the body fluid type and genotyping the body fluid donor cSNPs. Using the primer composition or kit provided by this invention, highly consistent genotyping results can be obtained, with high sensitivity and good tissue specificity. It provides an effective detection tool for directly associating common body fluid types and body fluid donors, and is of great significance for further research on body fluid identification and achieving accurate and efficient forensic identification related to body fluids.

The embodiment of the application discloses a kind of information determination methods, comprising: after obtaining the forensics operation of user, the forensics identification of forensics operation, the forensics position of user and the visible range of user are determined and sent to server;Obtain the information available to user in terminal and get evidence file;Determine the file identification of evidence file, and store file identification and forensics identification to target blockchain, file identification and forensics identification have mapping relationship;Receive the user feature of user to be confirmed sent by server, and the user feature is determined based on the forensics identification, forensics position and the visible range of user by server;Determine the target user feature and target storage information of target user based on user feature, and send to server, to make server determine the target identification of target user based on target user feature and target storage information.The embodiment of the application also discloses a kind of information determination device, equipment, computer storage medium and computer program product.

The invention relates to the technical field of molecular detection, in particular to forensic body fluid identification based on GC-MS metabolites. By means of the identified metabolite, body fluid type identification under the low sample size in a forensic identification scene can be achieved, the method has the advantages of being high in sensitivity, good in reproducibility, nondestructive in detection and the like, and a brand new thought is provided for forensic physical evidence identification.

This invention relates to the fields of molecular biology and forensic identification, specifically to a method for bird species identification based on multi-gene co-amplification and nanopore sequencing. The method involves extracting genomic DNA from avian biological samples; using the extracted DNA as a template, amplification is performed using four independent PCR primer pools that specifically target the avian mitochondrial cytochrome C oxidase subunit I gene, cytochrome B gene, 12S rRNA gene, and 16S rRNA gene; after purification of the amplification products, a nanopore sequencing library is constructed and sequenced; finally, bioinformatics analysis, including read length clustering, draft consensus sequence generation, and polishing, is performed on the raw sequencing data to obtain consensus sequences for each target gene. Species identification is then completed by comparing the sequence with a avian sequence database. This method effectively solves the problem that existing technologies cannot simultaneously meet the practical needs of large-scale, rapid, and high-success-rate identification of avian biological samples.

The invention provides a genetic marker detection system containing 68 high-performance autosomal haplotypes and application, and relates to the field of forensic identification. The detection system is formed by combining 68 amplification primer pairs after amplification, and the sequences of the amplification primers are as shown in SEQ ID No. 1 to SEQ ID No. 136. The invention also provides a method for identifying the genetic relationship, which comprises the following steps: S1, collecting a sample, extracting DNA (Deoxyribonucleic Acid), and obtaining a corresponding original sequencing result; s2, comparing an original sequencing result with a human reference genome hg38, and screening to obtain a genetic typing of each MiniHap gene locus; and S3, calculating the likelihood ratio of the specific genetic relationship between the sample pairs, and judging whether the sample pairs have the genetic relationship or not. According to the invention, a haplotype panel containing 68 human MiniHaps gene loci is constructed, and by combining multiple PCR and nanopore sequencing, very high personal identification and genetic relationship identification system efficiency can be achieved, and genetic relationship identification within third level is expected to be realized.

This invention belongs to the field of forensic identification technology. It provides a method and system for automatic annotation of forensic injuries based on image segmentation technology. The method includes: an automatic annotation device capturing video images of the wound area targeted in the forensic examination; using image segmentation technology to extract the external shape data of the wound from the video images; a posture prediction model analyzing and processing the external shape data to predict the target's display posture; determining the target shooting angle based on the target's display posture; while the forensic examiner displays the wound area of ​​the target object, the automatic annotation device capturing high-definition images of the displayed wound area according to the target shooting angle; extracting wound feature data from the high-definition images; assessing the injury severity level of the wound area based on the wound feature data; automatically annotating the injury severity level in the high-definition images; and saving the data. This method enables automatic assessment of wound severity levels and automatic annotation in high-definition images, improving the efficiency of forensic injury examination.

The invention relates to an STR marker combination for dogs and application of the STR marker combination, and belongs to the technical field of molecular biology and forensic identification. The STR marker combination comprises a combination of any four STR gene loci in the following 20 gene loci: PEZ1, PEZ2, FH2010, PEZ5, PEZ12, FH2309, PEZ20, PEZ15, FH2079, PEZ8, FH3377, PEZ21, PEZ3, PEZ6, FH2004, FH2054, VWFX, FH2611, FH2132 and FH2328, and a combination of any four STR gene loci in the following 20 gene loci. The STR marker combination further comprises a gene locus Amel for identifying sex. Through reasonable gene locus and fragment combination and fluorescent combination, the problem of simultaneous PCR amplification of 21 fluorescent primer single tubes is solved. And secondly, the technical verification index of the 10 [mu] L reaction system completely meets the forensic medicine application requirements, and the cost is greatly reduced. And finally, through cooperation of multiple departments, an STR database of 1000 kinds of dogs in the Chengdu region based on the system is constructed for the first time, and the application basis for effectiveness calculation is solved.

The invention discloses a genetic marker system containing 31 high-performance autosomal microhaplotypes as well as a detection primer and application thereof, and belongs to the technical field of forensic medicine identification. The genetic marker system comprises 31 loci, namely, mh02ZL-022, mh03ZL-014, mh03ZL-015, mh03ZL-016, mh03ZL-017, mh03ZL-018, mh04ZL-014, mh10ZL-010, mh10ZL-01, mh10ZL-012 and the like, and is characterized in that the molecular weight of the genetic marker system is 8000-9000, and the molecular weight of the genetic marker system is 8000-9000. On the basis of traditional micro-haplotype screening, InDels is incorporated into micro-haplotypes for whole genome screening, and an amplification system of a novel composite genetic marker is constructed. The system has the advantages of short fragment, short amplicon, low mutation rate and the like, is wide in applicability, and has good application potential in individual recognition, genetic relationship analysis and mixed sample detection of forensic detection.

This invention discloses a method for identifying peanut oil components and its applications. The invention screens and identifies three peanut oil-specific markers: alfalfa ketone, stigmatine, and chiricanine A. Based on these three markers and their characteristic ion pairs, peanut oil components can be accurately identified qualitatively using liquid chromatography-tandem mass spectrometry, with a detection limit of 1% (mass ratio). The method provided by this invention has the advantages of high accuracy, good specificity, and applicability to the detection of various vegetable oils, and can be widely applied in fields such as food authenticity identification, quality supervision, and related forensic identification.

The utility model discloses a kind of pupil measuring instruments for forensic identification, including pupil measuring instrument, support assembly is set on pupil measuring instrument, support assembly includes two threaded columns, two threaded columns are respectively installed on the lateral wall of pupil measuring instrument, threaded column is movably sleeved with first ring and support part, support part side installs moving part, support part includes second ring, second ring is movably sleeved on threaded column, moving part is set in second ring lateral wall, second ring outer wall is provided with telescopic plate.The utility model moving part's connecting plate one end is set on support part, connecting plate other end installs pull ring, forensic identification is moved by pulling pull ring and driving connecting plate, single hand completes the position adjustment of support part, vacates another hand and fixes patient head or operates other equipment, improve work efficiency, first ring and second ring are sleeved on threaded column, cooperate with support rod and form triangular support structure, enhance overall rigidity, prevent deformation or wobble in use process.

The invention discloses a composite system and method for skin identification and application, and belongs to the technical field of forensic medicine identification. The kit comprises a primer combination for amplifying a detection site, the sequences of the primer combination are as shown in SEQ ID NO.1-30; and the detection sites comprise cg06833110, cg14542120, cg05942459, cg02745009, cg02807788, cg10959820, cg17320707, cg25855753, cg22226904 and cg20603548, and the detection sites comprise the following components: the cg06833110, the cg14542120, the cg05942459, the cg02745009, the cg02807788, the cg10959820, the At present, no ARMS-PCR composite system for skin identification exists, a body fluid sample is identified and analyzed by adopting an ARMS-PCR technology, a DNA methylation composite system for detecting skin components is constructed, and the DNA methylation composite system has excellent sensitivity and specificity.

This invention discloses a method and system for estimating the time of bloodstain traces in judicial practice, relating to the fields of forensic identification and physical evidence technology. The invention acquires spatial distribution AC magnetic susceptibility tensor data of bloodstains and pure substrate magnetic signal array data; constructs a background noise covariance matrix and inputs it into an adaptive Wiener filter model to perform deconvolution separation, generating pure bloodstain intrinsic magnetic susceptibility spectrum data stripped of background interference; performs nonlinear fitting processing on the spectrum data using the fractional-order Haveryak-Nicaraguan relaxation equation, analytically generating relaxation dynamics feature set data; maps this data to a high-dimensional space of a Riemannian manifold to perform geodesic distance optimization calculation, outputting the bloodstain retention time corresponding to the minimum distance. This invention achieves non-destructive, non-contact detection, completely eliminates magnetic field interference from complex substrates, overcomes the measurement distortion of high-dimensional biochemical features, and significantly improves the accuracy and robustness of time estimation.

The present application relates to the field of forensic pathology, forensic genetics and molecular identification technology, in particular to a primer composition for evaluating genetic susceptibility of unexplained sudden death, products and applications thereof. The primer composition provided by the present application comprises nucleotide sequences such as amplification primers shown in SEQ ID NO. 1-512, that is, 256 pairs of primers related to 238 quantitative trait loci. The present application further provides products and methods for evaluating genetic susceptibility of unexplained sudden death of individuals. Experimental results show that the primer composition or kit provided by the present application can detect consistent and highly repeatable genotyping results, and on this basis, the genetic susceptibility of unexplained sudden death can be further evaluated by calculating the polygenic risk score, which has important significance for the genetic background research of unexplained sudden death and the accurate cause of death identification in sudden death related cases in forensic identification work.

Disclosed is a method for identifying multiple skulls and different subjects, using (i) a 3D model of each skull and (ii) one or more clear photos of the face of each candidate subject, wherein the method comprises the steps of: detecting craniometric points on the 3D skull models; estimating the thickness and direction of the soft facial tissue; detecting cephalometric landmarks in the face photos; and filtering on the basis of morphometric consistencies between the skull and the face, craniofacial superimposition in multiple photos, and decision making.

This invention discloses a fingerprint development reagent based on natural AIE compounds and precipitated silica, its preparation method, and its application, belonging to the field of forensic identification technology. The reagent is a particulate suspension comprising precipitated silica, natural AIE compounds, a surfactant, and a solvent. During preparation, the surfactant is dissolved in the solvent, the natural AIE compound is added and dissolved, and then precipitated silica is added in batches with stirring to obtain a uniform and stable suspension. This invention uses naturally derived AIE active substances, which are non-toxic, environmentally friendly, and low-cost, avoiding the biotoxicity and complex preparation process of traditional synthetic dyes. It also effectively solves the problems of dust pollution and poor compatibility with moist objects in powder development methods. It achieves high-contrast, high-definition fluorescence development of latent fingerprints on both dry and moist, non-permeable surfaces. The preparation process is simple and controllable, easy to scale up, and has good application prospects in rapid on-site detection fields such as criminal investigation and forensic identification.

The invention discloses a 28 Multi-InDel marker composite system based on an eight-color fluorescence detection technology, a detection primer and application, and belongs to the technical field of forensic medicine identification. According to the invention, the eight-color fluorescence technology is applied to Multi-InDel detection for the first time, 28 autosomal short segment site genetic marker systems are integrated, and the amount of detection information is significantly increased. The system has excellent adaptability to highly degraded DNA samples, the detection rate is high, the extremely low cumulative matching probability (2.3292 * 10 <-18 >) and the extremely high cumulative exclusion probability (0.9999669) are shown, and an efficient and reliable technical scheme is provided for forensic degraded sample identification.

The invention discloses a gene polymorphic site detection kit for predicting the curative effect of warfarin drugs. The kit comprises a MassARRAY chip and six primers for detecting rs72558192 and rs769942899SNP (Single Nucleotide Polymorphism) sites of a gene CYP2C9, the six primers comprise a forward primer, a reverse primer and an extension primer, the sequences of the forward primer, the reverse primer and the extension primer are respectively shown as SEQ ID No.1-6, and the primers are used for identifying polymorphic sites of warfarin drug effect related genes. The kit disclosed by the invention can be used for carrying out high-throughput detection on the two sites, and has the advantages of high sensitivity, high specificity, stable detection result, high reliability and the like; meanwhile, the method is suitable for gene analysis fields such as clinical disease mutation detection, pharmacogenomics analysis and forensic medicine identification.

The invention discloses an antibacterial wear-resistant composite coating for biological sample drilling and a preparation method thereof, and belongs to the technical field of surface coatings, the composite coating sequentially comprises a CrN binding layer, a TiAlN / CrN nano multilayer hard layer, an Ag / Cu doped TiAlN antibacterial functional layer and an a-C: H diamond antifouling surface layer from a substrate to the outside, the nano multilayer structure hardness is 30-35 GPa, the antibacterial rate is greater than 99.9%, and the antibacterial wear-resistant composite coating has the antibacterial wear-resistant function. The friction coefficient is less than 0.15, and the surface energy is less than 35 mN / m. The device is suitable for drilling hard biological samples such as bones, teeth, turtle shells and fossils, the service life of a tool can be prolonged by 5-8 times, inhibition of metal ions on PCR is eliminated, cross contamination of microorganisms is prevented, and the requirements of the fields of customs quarantine, forensic identification, ancient DNA research and the like are met.

The invention belongs to the technical field of molecular markers, and particularly relates to a multiple fluorescent quantitative PCR (polymerase chain reaction) detection method and a kit for distinguishing Chinese pangolin, manis pentadactyla and South African pangolin varieties. Three groups of specific primers and probes are utilized to distinguish different varieties of pangolin scales, including Chinese pangolin scales, malayan pangolin scales and South African pangolin scales. Specific gene segments of three species are amplified at the same time through one qPCR reaction, and the method has the advantages of being high in specificity and sensitivity, easy and convenient to operate and low in cost. The method is suitable for pangolin species identification, forensic identification, customs examination and biological protection research.

A method provides for simulating and training for visual inspection operations in virtual or augmented reality environments, suitable for use in simulating investigation reports, evidence inspection or forensic examination. Said method includes the interaction of a user with one or more objects. The interactions with an object can be either direct or indirect, or can include one or more operations of marking the environment. Said interactions are detected by actuators and / or sensors and are represented on visual display equipment, thereby allowing the user to view the same. Lastly, the operations performed by the user are compared to a sequence of reference operations, the user receiving a score on the basis of the same. A system is provided for implementing the aforementioned method.

The application provides a biomarker combination specific to coronary sudden death and application and products thereof, and belongs to the technical field of forensic science.The biomarker combination comprises ascorbic acid, docosahexaenoic acid, carnitine, glutamine and norleucine.The biomarker combination is significantly changed in serum samples of individuals with coronary sudden death, and after ROC verification, shows good diagnostic performance, indicating that it has the potential to be applied to forensic identification of coronary sudden death.The potential application value of the non-targeted metabolomics strategy of the application in difficult and complex death cause identification provides a new idea for molecular diagnosis of death cause investigation.

The application relates to the field of forensic identification, and discloses a microhaplotype genetic marker system for mixed stain inspection and a method and application thereof. The system contains 135 microhaplotype sites of autosomes and corresponding amplification primers, and a microhaplotype typing system based on MPS technology is constructed, which has good sensitivity, specificity and inhibitor tolerance. Further combined with a microfluidic chip, automatic capture and whole genome amplification of single cells are realized, and the average locus detection rate of single cell samples reaches 83.48% in cooperation with a short fragment microhaplotype marker system, and individual sources in complex mixed stains can be accurately distinguished. The system can be widely applied to scenes such as individual identification, kinship identification and mixed stain analysis in forensic science.

The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid target sequence detection method and system based on solid phase hybridization and enzyme digestion recognition and application. The method comprises the following steps: preparing target nucleic acid with a detectable marker; hybridizing with a specific probe fixed on a solid-phase carrier; treating the solid phase carrier with an enzyme treatment system comprising a cleavage mismatched endonuclease to specifically remove hybrid double strands that are not completely matched with the probe; and finally, detecting a mark signal reserved on the carrier so as to judge the state of the target sequence. According to the method, an enzyme digestion verification step is introduced after traditional solid-phase hybridization, and non-specific hybridization is eliminated from a molecular level by utilizing high recognition capability of enzyme on nucleic acid mismatch or structural distortion, so that ultrahigh specific detection close to single base resolution is realized. The technical platform is high in universality, and can be applicable to detection of various types of targets such as single nucleotide polymorphism (SNP), point mutation, short tandem repeat (STR) and pathogen genes only by replacing the probes. The method is simple and convenient to operate, can be easily integrated with isothermal amplification, test strips and other simple carriers, is suitable for on-site rapid detection, and has wide application prospects in the fields of molecular diagnosis, forensic medicine identification, pathogen monitoring and the like.

The invention relates to a single-point rapid mass spectrometry quantitative method based on composite internal standards, which comprises the following steps: selecting one or more composite internal standards, setting the concentration of the composite internal standards, and selecting the concentration of the composite internal standards in a linear interval of a substance to be detected; when the number of the composite internal standard is one, the charge ratios of the internal standard and the substance to be detected are different; adding the internal standard into the to-be-detected sample, constructing a linear model passing through the original point and the internal standard data point, and obtaining the concentration of the to-be-detected sample; when the number of the composite internal standards is multiple, the mass-to-charge ratios of the to-be-detected object, the first internal standard to the nth internal standard are different; the internal standards with different concentrations are added into a to-be-detected sample, and the concentration of the to-be-detected sample is obtained by constructing a mass spectrum peak response value linear model of the internal standards. The method overcomes the defects that a traditional method needs multi-point calibration and needs long time, the detection efficiency is remarkably improved while the accuracy is guaranteed, and the method is particularly suitable for a small portable mass spectrometer in high-throughput rapid detection scenes such as acute poisoning, judicial expertise and food and drug safety supervision.