Hair stem cracking liquid

A lysate and hair technology, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of long digestion time and low efficiency of hair, and achieve the effect of shortening digestion time and improving digestion efficiency

Inactive Publication Date: 2009-08-19
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of long digestion time and low efficiency of the existing hair, and p...

Method used

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  • Hair stem cracking liquid
  • Hair stem cracking liquid

Examples

Experimental program
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Embodiment 1

[0008] Tris-Cl, NaCl, CaCl at pH 8.0 2 .

[0009] Table 1 The content of each component of the dry lysate of the present invention and the digestion time

[0010]

[0011] Take fresh hair with a length of 40cm to dry, first fully wash with detergent to remove impurities on the hair, rinse with sterile distilled water several times, then wash with sterile 2% NaOH for 15 minutes, then use sterile 2% NaOH Wash with HCl for 15 minutes, rinse with sterile distilled water several times, wash with 75% and 100% ethanol successively, and dry it for later use.

[0012] Cut the fragments about 0.5-3mm long under sterile conditions, and put them in 4 numbered C 1 ~C 4 And in sterile EP tubes with a volume of 2ml, the length of the hair shaft in each EP tube is 10cm.

[0013] in C 1 ~C 4 Add 400 μl of digestive solution at 56°C to the EP tube according to Table 1, mix thoroughly, and bathe in 56°C water until no obvious hair fragments can be seen in the EP tube. During this period...

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PUM

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Abstract

The invention discloses hair cracking solution. The cracking solution comprises the following proportions of components: 10mmol/L of Tris-Cl (pH is 8.0), 100mmol/L of NaCl, and 1mmol/L of CaCl2, 20-200mmol/L of DTT, 0.5%-5.0% of SDS (mass concentration), 0.5%-4% of Triton X-100 by volume and 0.2-2mg/ml of protease K, wherein, a solvent is sterile double-distilled water. Compared with the existing hair digestive juice, the cracking solution has the advantages of largely shortened digestion time and greatly improved digestive efficiency, and the cracking solution provides a new way for forensic identification and clinical diagnosis.

Description

technical field [0001] The invention relates to biochemical reagents, in particular to a hair lysate. Background technique [0002] Hair is one of the most common crime scene evidence during crime scene evidence collection. In addition, because the hair shaft has a special molecular structure that makes it non-perishable, it is easier to collect than other crime scene evidence such as blood, saliva, fingerprints, footprints, muscles, etc., especially in crime scenes that have been committed for a long time . At present, in the process of forensic identification, the genetic information of the criminal suspect is obtained from the evidence at the scene, and the molecular comparison is used to determine whether the criminal suspect participated in the crime. In the process of extracting genetic information from the hair shaft as a sample, workers often find that the hair shaft is not easy to digest. The current fastest digestion method still takes 3 hours or even longer. Th...

Claims

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Application Information

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IPC IPC(8): C07H21/00C07H1/08
Inventor 罗学港黄菊芳王慧曾乐平陈旦童建斌
Owner CENT SOUTH UNIV
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