Determination method of protein content in foodstuff

A protein content and determination method technology, applied in the field of food testing, can solve the problems of high cost, high hazards of operators, complicated and cumbersome operations, etc., and achieve the effects of reducing hazards, eliminating air pollution, and speeding up analysis

Inactive Publication Date: 2012-02-01
SHANGHAI HAIDI GARDENING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the technical problems of complex and cumbersome operation of the determination method of protein content in food in the prior art, great harm to operators, and high cost

Method used

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  • Determination method of protein content in foodstuff

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Embodiment

[0022] The digestion device used in this embodiment is: replace the Kjeldahl flask with a 100ml pear-shaped three-neck flask, connect the dropping funnel, the vacuum regulator tube and the elbow respectively, and the catheter for the elbow and the suction filter bottle containing the dilute alkali solution It is connected with a vacuum pump to absorb the flue gas produced by digestion with lye.

[0023] The reagent used in the embodiment is:

[0024] Concentrated sulfuric acid was purchased from Sinopharm Chemical Reagent Co., Ltd.;

[0025] 8.8mol / L hydrogen peroxide was purchased from Kaifeng Kaihua Group Co., Ltd.;

[0026] Sodium hydroxide was purchased from Zibo Chemical Reagent Factory;

[0027] Formaldehyde was purchased from Zibo Chemical Reagent Factory;

[0028] Hexamethylenetetramine was purchased from China Institute for the Control of Pharmaceutical and Biological Products;

[0029] Phenolphthalein indicator was purchased from Shanghai No.3 Reagent Factory;

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Abstract

The invention relates to a determination method of the protein content in foodstuff in the technical field of food detection, comprising the following steps of: digesting a sample by using a digestive juice of hydrogen peroxide and concentrated sulfuric acid so as to obtain a mixed solution; taking the mixed solution, using methyl red as an indicator, and adding a NaOH aqueous solution; adding a formaldehyde solution, shaking, adding a phenolphtalein indicator, titrating by the use of a NaOH aqueous solution at the concentration of C until the mixed solution shows reddish and does not fade in color within half a minute, recoding the volume V of the consumed NaOH aqueous solution, and calculating the protein content by the use of a formula. The digestive juice used in the method can help accelerate the digestive speed of the sample, shorten the digestive time and accelerate the analysis speed; in addition, the formaldehyde method is adopted for titration so as to simplify the operation.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for measuring protein content in food. Background technique [0002] Protein is an important component of living body cells. Protein in food is the only source of nitrogen in the human body, and it plays an irreplaceable role in biological macromolecules such as sugar and fat. Protein is closely related to nutrient metabolism, cell structure, enzymes, hormones, viruses, immunity, material transport and genetics in organisms, and its separation and qualitative or quantitative determination are of great significance in food inspection. There are many methods for determining the protein content in food, which can be generally divided into indirect methods and direct methods. The indirect method is to calculate the protein content by measuring the nitrogen content of protein in the sample; the direct method is to directly measure the protein according to the physical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/79
Inventor 郭景鹏沙飞翔丁国才
Owner SHANGHAI HAIDI GARDENING
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