141results about How to "Reagent saving" patented technology

The invention provides a preparation method for a molecular imprinting material and the molecular imprinting material prepared through the preparation method. The preparation method comprises the steps that silicon oxide is coated on the surfaces of magnetic ferroferric oxide nanometer particles, the magnetic ferroferric oxide nanometer particles are modified with gamma-(methacryloyl chloride) amino propyl trimethoxy silane to obtain magnetic ferroferric oxide nanometer particles with propenyl on the surfaces, the magnetic ferroferric oxide nanometer particles with the propenyl on the surfaces serve as carriers, estrogen receptors are simulated, functional monomers are optimized, a surface imprinting technology is adopted, and then the molecular imprinting material which can simultaneously identify seven kinds of environmental endocrine disrupting chemicals is prepared. According to the preparation method, the easy separation of a magnetic nanometer material, the good water solubility of a silicon oxide nanometer material, the specific recognition ability of molecular imprinting polymers and the surface imprinting technology are mutually combined, the preparation technology is simple, the conditions are mild, the prepared molecular imprinting material is large in adsorption capacity, fast to respond, high in magnetism, good in chemical stability and high in repeating utilization rate, and the problems that at present, multiple trace, steroid and phenol environmental endocrine disrupting chemicals are difficult to simultaneously identify, separate and enrich are solved.

The invention discloses an Ag/TiO2/graphene nanometer composite photocatalyst and a preparation method thereof, belonging to the field of new materials. The Ag/TiO2/graphene nanometer composite photocatalyst is prepared by assembling metal silver, nanometer titanium dioxide (P25) powder onto a two-dimensional lamellate graphene carrier material by using a photocatalytic oxidation-reduction method, using the two-dimensional lamellate graphene as the carrier material, the silver nitrate as the silver source and the nanometer titanium dioxide as the photocatalysis material, initiating the nanometer titanium dioxide to generate photoproduction electron and photoproduction hole by stimulating sunlight, capturing the photoproduction hole by a sacrifice agent, and restoring the silver ion and the graphite oxide to be metal Ag and graphene by the photoproduction electron. The invention has simple preparation method and low cost in reagent, is beneficial to large-scale preparation, and provides an environment-friendly method for preparing graphene load composite photocatalyst through the restoration of the graphite oxide.

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

The invention discloses a graphene load tungsten trioxide (WO3) nanowire composite material and a preparation method thereof, which belong to the field of new materials. The nano composite material has a one-dimensional composite structure and a two-dimensional nano composite structure, the diameter of a WO3 nanowire is 10-30 nanometers, the length of the WO3 nanowire is 50-600 nanometers, and the WO3 nanowire penetrates through or is distributed on the inner layer or the surface of a layer-shaped grapheme main material. The preparation method comprises utilizing a two-dimensional grapheme as an auxiliary material and sodium tungstate as a tungsten source, generating the WO3 nanowire through a hydrothermal synthesis method, then mixing the WO3 nanowire with graphite oxide dispersing solution, and then obtaining the graphene load WO3 nanowire composite material by means of photocatalytic reduction. The preparation process is simple, reagents are cheap, large-scale preparation is facilitated, and simultaneously an environment-friendly preparation method is provided for reduction of graphite oxide and formation of the nano composite material.

The invention discloses a novel blood corpuscle analysis meter and a novel blood corpuscle analysis method, which comprises a detecting system which consists essentially of a feeding and cleaning system, a microscope platform, a CCD camera, a drive circuit and a single chip microcontroller, is used for obtaining morphological information of blood corpuscles and is connected with a computer system, collected information is connected into a computer through a sampling card, image analysis processing is implemented, classifying and counting of the blood corpuscles are implemented from the aspect of cellular morphology, and an analysis result is output through the output device of a monitor and a printer. The novel blood corpuscle analysis meter and the novel blood corpuscle analysis method simulate the procedure of manual microscopical examination, provide a new concept for developing blood corpuscle analysis meters, are characterized by strong universality and reagent saving effect, provide an automatic method for morphological blood corpuscle detection, and can reduce the working load of manual microscopical examination.

The invention discloses a cell chip slide for preparing microarray cell chips. The cell chip slide comprises a substrate and a sealing cover, the substrate is provided with array holes distributed in an array manner, the bottoms of the array holes are transparent and positioned on the same horizontal plane, the sealing cover is provided with hollow array posts matching with the array holes of the substrate, the array posts can extend into corresponding array holes, and the bottoms of the array posts are transparent and positioned on the same horizontal plane. The cell chip slide is simple in structure, easy to prepare and convenient to use, and can comprise various (numerous) cells arrayed in a regular array manner, therefore, detection or experimental studies of same or different indexes can be performed for various (numerous) cells simultaneously once. By the aid of the cell chip slide, operation steps are simple, and detection or experimental studies can be completed once.

The invention relates to a method for detecting human beta-globin gene mutation, in particular to a method for detecting the beta-globin gene mutation by a modified molecular beacon melting curve. The method comprises the following steps of: designing and preparing a corresponding modified molecular beacon in region of a beta-globin gene needing mutation detection; designing an upstream primer and a downstream primer on the periphery of the designed modified molecule beacon, and performing PCR amplification on fragments containing the region to be detected by using the upstream primer and the downstream primer; and after PCR amplification is finished, analyzing the melting curve, and judging whether a nucleotide sequence to be detected has the gene mutation and possible mutation types according to the change of the melting point of the modified molecular beacon.

The invention discloses a super-hydrophobic hollow Fe3O4/mesoporous silicon dioxide nanocomposite which is of a core-shell structure. Hollow Fe3O4 nano microspheres serve as magnetic cores, SiO2 serves as shells, the particle size of the cores ranges from 220 nm to 260 nm, the thickness of the shells ranges from 35 nm to 50 nm, a SiO2 shell layer is of a mesoporous structure, and the surface of the composite is modified with vinyl and methyl. The invention further provides a preparation method of the composite. The method comprises the steps that firstly, the hollow Fe3O4 nano microspheres are ultrasonically dispersed in an isopropyl alcohol solution, then NH3.H2O is added at room temperature, and after the mixture is stirred evenly, tetraethyl orthosilicate is slowly added for several times; secondly, after a reaction is competed, washing is performed, then the mixture is dispersed in deionized water, and under the protection of polyvinylpyrrolidone K15, NaOH is added for surface etching; thirdly, the surface hydrophobicity is modified through vinyl trimethoxy silane, and the super-hydrophobic hollow Fe3O4/mesoporous silicon dioxide nanocomposite is finally obtained. The composite is light and porous, can effectively absorb oil and lock oil, can be recycled and reused and has a great application prospect in the aspect of oil pollution treatment.

The invention discloses a preparation method of a carbon-based quantum dot/nano-silver surface enhanced raman base. The preparation method comprises the following steps: dissolving carbon-based quantum dots and silver ions in a water solution; adding sodium boro-hydride serving as a reducing agent under a stirring state, so as to prepare a carbon-based quantum dot/nano-silver composite material. The preparation method is simple, convenient, easy, free of pollution, fast in reaction, and high in operability. As the carbon-based quantum dots on the surface of the carbon-based quantum dot/nano-silver composite material comprise a large quantity of oxygen-containing functional groups, the carbon-based quantum dot/nano-silver composite material is favorable in dispersity and long-lasting in stability in water. More importantly, as the carbon-based quantum dot/nano-silver composite material comprises a large quantity of 'notches', the surface enhanced raman activity is excellent; as the carbon material on the surface of the carbon-based quantum dot/nano-silver composite material can adsorb benzene series via electrostatic adsorption and Pi-Pi function, the carbon-based quantum dot/nano-silver surface enhanced raman base can be applied to surface enhanced raman detection of the benzene series.

The invention belongs to the technical field of medicines, and relates to a two-aqueous-phase method for extracting total saponin from bittersweet herb. The Two-aqueous-phase method for extracting total saponin from bittersweet herb is characterized by comprising the following steps of: pulverizing bittersweet herb, adding to a solvent, carrying out solid-liquid digestion and then filtering for impurity removal to obtain a coarse extract of the bittersweet herb; concentrating the coarse extract of the bittersweet herb to 1/5 of the original volume, adding to a two-aqueous-phase system formed from absolute ethyl alcohol/ammonium sulfate, adding water to ensure that the total amount of the system is a certain value, uniformly mixing and then standing to form an upper phase and a lower phase; and taking out the absolute ethyl alcohol phase containing a great amount of total saponin of the bittersweet herb, and then carrying out pressure-reduction concentration on the absolute ethyl alcohol phase obtain a total saponin extractive of the bittersweet herb. The two-aqueous phase method has the beneficial effects that the extraction rate of the total saponin of the bittersweet herb is improved; as the used solvent has low toxicity, the method is beneficial to environmental protection; and simultaneously the method has convenience and economy.

The invention discloses a virus nucleic acid extraction kit. The virus nucleic acid extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution, an elution solution,protease K and magnetic beads; the lysis solution comprises sodium perchlorate with the concentration of 1.0-5.0 mol/L, Tris-HCl with the concentration of 10-200 mmol/L, EDTA with the concentration of 1-50 mmol/L, and Triton X-100 with the concentration of 50-100 microlitre/milliliter; the first rinsing solution is sodium perchlorate solution with the concentration of 2.0-2.5 mol/L, and the solvent of the sodium perchlorate solution is 70-80% ethyl alcohol; the second rinsing solution comprises DEPC water and absolute ethyl alcohol, wherein the volume ratio of the DEPC water to the absolute ethyl alcohol is (3-4):(6-7); the elution solution is Tris-HCl-DEPC water solution with the concentration of 8-12 mmol/L. The invention further discloses a method for extracting viral nucleic acid by using the extraction kit. Through optimization of the reagent formula, the extraction kit has the advantages of being rapid and efficient and avoiding pollution, a large amount of time and reagent canbe saved, and the extraction efficiency of the virus nucleic acid is improved.

The invention discloses a Chinese herbal medicine preservative. The preservative is prepared by mixing 7.3-23 parts of traditional Chinese medicine extract, 1.2-5 parts of functional accessory and 72-91.5 parts of water by weight, wherein the traditional Chinese medicine extract is a mixture of flos lonicerae, radix isatidis, radix scutellariae and licorice extract; and the functional accessory is a mixture of vitamin C and kojic acid. The Chinese herbal medicine preservative disclosed by the invention has the characteristics that the refreshing time can be effectively prolonged, possible adverse effects on human bodies caused by the existing chemical preservative in use are avoided, the application range is wide, and the traditional Chinese medicine preservative is particularly suitable for the fruits and vegetables with high water content and vigorous metabolism.

The invention discloses a method for in-situ distillation and on-line measurement of Os in a Carius tube. The method comprises the following steps: a sample is decomposed by inverse aqua regia in a Carius tube method, the generated OsO4 steam enters into a carrier gas port of an atomizer under carry of Ar gas through a special interface, Ir solution is introduced into a liquid inlet of the atomizer, and mixed aerosol comprising Os and Ir is formed at a nozzle of the atomizer. According to the invention, in comparison with the prior art, the Ar gas enters into the carrier gas port of the atomizer along with the Os steam and reaches a rectangular pipe, and the Ir standard solution introduced from the liquid inlet pipe is used for on-line discrimination and correction, so that the measuring precision is improved; after the sample is dissolved by the Carius tube, the in-situ distillation is directly carried out without separation, and meanwhile, a ratio and a content of an Os isotope can be obtained by on-line precise measurement. In comparison with the original on-line measurement method, the method, provided by the invention, is difficult to put out a fire, and in comparison with a solution feeding measurement method, a memory effect is remarkably reduced.

The invention relates to a method for sheeting peanut root tip cell chromosome in the mitosis metaphase, which comprises the following steps that: a peanut root tip is selected, and paradichlorobenzene is utilized for soaking pretreatment; the root tip is put into carnoy stationary liquid for room temperature treatment; acetic acid is used for soaking, the root tip of a meristematic zone is cut and put on a glass slide, the center of a cover glass is tapped to disperse the cells, a cell wall is heated by fire for dissociating and softening, and a filter paper is used for removing the excess acetic acid; the cell which is in the mitosis metaphase and has smoothly dispersed chromosome is sheeted and refrigerated, the cover glass is removed, and the glass slide is dehydrated; and stain zoning or fluorescence in-situ hybridization are carried out. The obtained sheet has leveled cells and dispersed chromosome, and more cells are in the metaphase; and the acetic acid medium is used for direct compression, the reagent has small influence to the chromosome, so the method is easy to operate and control, has simple steps and high sheeting efficiency. The method is suitable to not only peanuts but also sesame, paddy, corns, soybeans, green beans or tomatoes and other various plants, and has good operability.

The invention provides a kit for detecting cysticercus cellulosae, swine trichinella and swine toxoplasmosis. The kit comprises sterile deionized water, polymerase chain reaction (PCR) liquid, thermus aquaticus deoxyribonucleic acid (Taq DNA) polymerase, a GoldView DNA dye, bromophenol blue sample loading buffer solution, a standard substance and a reference substance. In the detection kit provided by the invention, DNA of the cysticercus cellulosae, the swine trichinella and the swine toxoplasmosis in a specimen to be detected can be detected quickly and accurately only by one reaction, and the kit can also be used for the molecular epidemiological survey and curative effect monitoring of cysticercus cellulosae, swine trichinella and swine toxoplasmosis infection. The method has simple preparation steps and is low in cost, easy to operate, time-saving and labor-saving, a large number of reagents and consumables are saved, and the work efficiency is improved. The method is high in detection sensibility, specificity and accuracy, and the false positive rate is reduced.

The invention discloses a method for detecting carbamate pesticide content in total particle matter in cigarette mainstream smoke, belonging to the technical field of tobacco chemical analysis. The method comprises the steps of putting a Cambridge filter with trapped total particle matter in cigarette mainstream smoke into a conical flask with a plug; adding 100ml of mixed solvent of ethyl acetate and cyclohexane according to the volume ratio of 85:15; putting the conical flask on an ultrasonic generator to extract for 30min; concentrating the extracted liquid to 0.5ml after drying; using the mixed solution of toluene and acetonitrile (1:3) to reach a volume of 2ml to be purified; putting Envi-carb/NH2SPE small column on a solid phase extracting device, adding 2g of anhydrous sodium sulfate to the Envi-carb/NH2 composite column; using 5ml of mixed solution of toluene and acetonitrile according to the volume ratio of 3:1 to perform activation; transferring the to-be-purified sample test liquid to the composite column, using 5ml of mixed solvent of acetonitrile and toluene according to the volume ratio of 3:1 to perform elution for three times, wherein the rate is not more than 5.0ml/min; concentrating the eluate to 0.5ml, using 5ml of dichloromethane to exchange solvents for two times, concentrating the exchanged test liquid to 1ml, and then performing analysis. The invention has the advantages of simple and convenient method and accurate detected data of pesticide content.

The invention discloses a method for extracting flavone component in hawthorn by using a pressurized liquid extraction (APLE) technique. The method comprises removing kernels from matured hawthorn fruits, conducting low-temperature drying, smashing by using a grinder, screening hawthorn powder with needed particle size, placing for reserving, weighting certain amount of the hawthorn powder to be sufficiently mixed with certain amount of kieselguhr, filling in an extraction pool, sealing the extraction pool, placing in a pressurized solvent extraction device, adding solvent, setting parameters and then automatically extracting. Maximum 12 samples can be automatically extracted by using a same method. The pressurized liquid extraction method is adopted, extracting time is short, conditions are mild, the solvent is saved, and flavone yield is high. The method further has the advantages of simple operation, good continuity and the like. Obtained hawthorn flavone can be used for further research and development of medical treatment, health care and functional foods.

The invention discloses a method for measuring the content of manganese in silicon-vanadium alloy. The method comprises the following steps of pretreatment, standard solution preparation and measurement, and concretely comprises the steps of feeding concentrated nitric acid and hydrofluoric acid into a sample to be measured, then feeding perchloric acid until smoking and being nearly dry, taking down, making up to volume, and shaking up for measurement; making a manganese spectral line intensity-mass fraction work curve on a plasma atomic emission spectrometer by the prepared standard manganese solution, and analyzing the sample to be measured through the curve to obtain the content of the manganese in the silicon-vanadium alloy. According to the method, the content of the manganese in the silicon-vanadium alloy is measured by a plasma atomic emission spectrometry; the method is less in the used reagent and low in interference, and is capable of reducing the environmental pollution and increasing the working efficiency; the method is good in accuracy and e provides accurate data for production and utilization of the silicon-vanadium alloy in the iron and steel enterprises. The method is convenient and rapid to operate and low in analysis cost, and measurement results of the method are good in stability, reproducibility and accuracy, so that the daily demand of measuring the content of the impurity element in the silicon-vanadium alloy can be met.

The present invention discloses a novel synthesis process of 5-amino-1-ethylol pyrazole and analog compounds thereof. In the process, acrylonitrile is used as a raw material, and the 5-amino-1-ethylol pyrazole and the analog compounds are synthesizsed through a certain process. The present invention has the advantages that the agents are cheap, the route of the synthesis process is simple, the operation is simple, the yield of synthesis is high, and the cost is low.

The invention discloses a bovine rotavirus, coronavirus, and viral diarrhea virus multi-connected RT-PCR detection method, which is characterized in that according to the detection method, RNA of a to-be-detected sample is taken as an RNA template, and a specificity upstream primer and a specificity downstream primer designed according to hereditary characteristics of the bovine rotavirus, coronavirus, and viral diarrhea virus are taken as a primer to perform RT-PCR proliferation to obtain a proliferation product. Then, electrophoresis detection is performed on the proliferation product with 1.5% agarose gel, and a result is recorded; according to the results of the electrophoresis detection, whether the to-be-detected sample contains the bovine rotavirus, coronavirus, and viral diarrhea virus is determined. The bovine rotavirus, coronavirus, and viral diarrhea virus detection method achieves has the beneficial effect of accurately, rapidly and efficiently distinguishing and diagnosingthe three viral disease with RT-PCR.

The invention discloses a genome DNA rapid extraction method suitable for the DNA amplification technology. The extraction method is quite simple and economic as only sodium hydroxide solution and Tris-HCl solution are employed. By employing the extraction method, DNA of a sample can be totally extracted just by 15 minutes. The extraction method is universal, and good extraction effects on decocting pieces of plant medicine and animal medicine, and processed products of the decocting pieces can be achieved. The quality of the extracted DNA meets demands of downstream PCR amplification, problems of long DNA extraction time and heavy workload in a traditional Chinese medicinal material common PCR reaction template are solved, and the method meets demands of DNA being rapidly extracted in scientific researches and production processes.

The present invention relates to a method for detecting nasopharynx exudate free heme. The method comprises sampling in nasopharynx, diluting the sample solution, adding detection reagents according to a certain proportion, and determining whether heme exists according to color change. The present invention further provides a special detection box for detecting nasopharynx exudate free heme, wherein the special detection box can be specially provided for holding a vessel and detection reagents used in the detection process. According to the present invention, the detection box has a simple structure, the whole detection step can be completed only through the one detection box, the space and the reagents are saved, and operator labor burden is reduced.