Detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis

A technology for active ingredients and osteoporosis, applied in the field of detection of active ingredients, can solve problems such as cumbersome operation, incomplete active ingredients, and inability to meet quality control

Inactive Publication Date: 2012-06-20
GUANGDONG MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, in the process of understanding the preparation process of Hugu Capsules, we found that the existing quality standards of Hugu Capsules are not comprehensive in testing the active ingredients, and the operation is cumbersome, which cannot meet the requirements of quality control.

Method used

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  • Detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis
  • Detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis
  • Detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Instruments and reagents.

[0044] Instrument: Agilent1200 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); Kromasil100-5C18 chromatographic column; Sartorius one hundred thousandth balance; KQ5200DB numerical control Ultrasonic cleaner; Milli-pore-Q ultrapure water preparation instrument.

[0045] Reference substances: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid, naringin.

[0046] Sample to be inspected: bone-protecting capsule.

[0047] Other reagents: acetonitrile (chromatographically pure), methanol, glacial acetic acid (analytical pure).

[0048] (2) Detection method.

[0049] a. Preparation of mixed reference substance solution: Accurately weigh 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid , an appropriate amount of naringin, dissolved in 100% methanol, constant volume, shaken, and filtere...

Embodiment 2

[0060] (1) Instruments and reagents.

[0061] Instrument: Agilent1200 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); Kromasil100-5C18 chromatographic column; Sartorius one hundred thousandth balance; KQ5200DB numerical control Ultrasonic cleaner; Milli-pore-Q ultrapure water preparation instrument.

[0062] Reference substances: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid, naringin.

[0063] Sample to be inspected: bone-protecting capsule.

[0064] Other reagents: acetonitrile (chromatographically pure), methanol, glacial acetic acid (analytical pure).

[0065] (2) Detection method.

[0066] a. Preparation of mixed reference substance solution: Accurately weigh 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid , an appropriate amount of naringin, dissolved with 80% ethanol, constant volume, shaken, and filtere...

Embodiment 3

[0077] (1) Instruments and reagents.

[0078] Instrument: Agilent1200 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); Kromasil100-5C18 chromatographic column; Sartorius one hundred thousandth balance; KQ5200DB numerical control Ultrasonic cleaner; Milli-pore-Q ultrapure water preparation instrument.

[0079] Reference substances: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid, naringin.

[0080] Sample to be inspected: bone-protecting capsule.

[0081] Other reagents: acetonitrile (chromatographically pure), methanol, glacial acetic acid (analytical pure).

[0082] (2) Detection method.

[0083] a. Preparation of mixed reference solution: Accurately weigh 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid , an appropriate amount of naringin, dissolved in 95% ethanol, constant volume, shaken, and filtered through a ...

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Abstract

The invention relates to a detecting method for traditional Chinese medicine components, in particular to a detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis, which is used for detecting effective components of Hugu capsules by means of multi-wavelength high-performance liquid chromatography. The detecting method includes the specific steps: a, preparing sample solution to be detected; b, preparing mixed reference sample solution; c, respectively detecting the sample solution to be detected and the mixed reference sample solution by means of high performance liquid chromatography; and d, comparing a high performance liquid chromatogram of the sample solution to be detected with that of the mixed reference sample solution. The detecting method can be used for simultaneously detecting chlorogenic acid, 2, 3, 5, 4'-tetrahydrostibene-2-O-beta-D-glucoside, ferulic acid, naringin and icariin and the content of the five effective components in the Hugu capsules. When the effective components in the Hugu capsules are detected by the method, high performance liquid chromatograms of standard substances of the five components are omitted, time and reagents can be reduced, and the detecting method is simple, convenient, high in precision and fine in reproducibility.

Description

technical field [0001] The invention relates to a method for detecting components of traditional Chinese medicines, in particular to a method for detecting effective components of traditional Chinese medicine Hugu Capsules for preventing and treating osteoporosis. Background technique [0002] Traditional Chinese Medicine Hugu Capsules are made of Polygonum multiflorum, Icariin, Rehmannia glutinosa, Tortoise shell, Morinda officinalis, Eucommia ulmoides, Dipsacus, Rhizoma drynariae, Angelica, Chinese yam, etc. It has the function of nourishing kidney and essence. Hugu Capsules are used for the treatment of lumbar spine pain, weakness, inability to hold weight, flaccid lower limbs, difficulty walking, or heel pain, dull complexion, hair loss, loss of libido, dizziness and tinnitus in middle-aged and elderly people with kidney essence deficiency syndrome. , Pale red tongue with thin white fur, red tongue with thin white fur; and patients with osteoporosis usually have the abov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 赵文昌宋丽军曾昭利
Owner GUANGDONG MEDICAL UNIV
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