119results about How to "The detection method is stable" patented technology

The invention provides a method for extracting and detecting a biotin of a bird nest. The method comprises the following steps of: extracting the biotin from the bird nest by using dimethylformamide; performing centrifugal purification and concentration to prepare a sample solution; by using a one-point external standard method, developing the sample solution and a biotin standard solution on a high-performance thin-layer plate coated with silica gel 60F254 by using a developer, wherein the developer is a mixture of chloroform, acetone, methanol and glacial acetic acid; spraying 0.05 percent of potassium permanganate solution to a developed plate for coloring; and drying the developed plate, and then performing thin layer scanning with absorption wavelength of 400 nm to obtain the contentof the biotin in the bird nest. The method has the advantages of simplicity, accuracy, quickness, stability and reliability, and the defects of complicated detection steps and high time consumption of the conventional microbiological method, and high investment cost, large solvent dosage and high cost of a high performance liquid chromatography are overcome.

The invention provides a traditional Chinese medicine composition of a bone healing medicine, a preparing method thereof and a detecting method thereof. Each preparation unit of the traditional Chinese medicine composition contains pseudo-ginseng which is no less than 2.2mg by total content of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 and / or Carthamus tinctorius which is no less than 0.03mg by content of hydroxysafflor yellow A, quality is more stable, and curative effect is more exact. According to the detecting method, the content of the notoginsenoside R1, the ginsenoside Rg1, the ginsenoside Rb1 and / or the content of the hydroxysafflor yellow A are detected through detecting methods including a high-performance liquid chromatography method. The method is simple and stable, high in accuracy and good in reproducibility, and capable of representing effective ingredient content of the traditional Chinese medicine composition of the bone healing medicine completely and effectively and benefiting monitoring product quality.

The invention provides a detection method of a Wuweiganlu preparation. The method comprises the following steps of: performing microscopic identification of the microscopic characteristics of juniperus formosana and myricaria in the Wuweiganlu preparation; performing thin-layer chromatography identification of rhododendron anthopogonoide, juniperus formosana and artemisia sieversiana in the Wuweiganlu preparation; and measuring the content of ephedrine hydrochloride and pseudoephedrine hydrochloride in ephedra and the content of artemisetin in artemisia sieversiana in the preparation by a high performance liquid chromatography. The detection method provided by the invention has the advantages of good reproducibility and stability, high precision, strong specificity, clear spot color, high separation degree, accurate content and the like, and is simple to operate; and by creating a reliable quality detection method with strong specificity, the quality of the Wuweiganlu preparation can be effectively controlled so that the quality of the Wuweiganlu preparation is stable, safe and controllable.

The invention discloses a method for extracting oestrone from a euphausia superba and an efficient thin layer chromatography scanning and detecting method. The method for extracting the oestrone from the euphausia superba comprises the following steps: extracting the euphausia superba as a raw material through methanol; and filtering, and steaming a filtrate to dry through a rotary evaporation instrument, so as to obtain a crude extract containing the oestrone. The oestrone of the euphausia superba is detected by using an efficient thin layer chromatography scanning method. A developing solvent is a mixture with a volume ratio of carbon tetrachloride to ethyl acetate to acetone to glacial acetic acid to methylbenzene to cyclohexane of (78-80) to (9-11) to (9-11) to (2-4) to 3d to 2d; a coloring agent is a 5% ferric chloride water solution; the content of the acetone can be obtained through calculation of absorbances of a detection sample product and a standard product by carrying out scanning detection with an absorption wavelength of 600nm by using a CAMAG thin layer chromatography scanner III. The method has the advantages of being simple to operate, fast, accurate and reliable.

The invention discloses a detection method of Yinqiao powder. The detection method can simultaneously determine the content of chlorogenic acid, forsythin, forsythiaside A and arctiin. The detection method has the advantages of high efficiency, fastness, high accuracy, high sensitivity, good repeatability, reliable result, effective control of the quality of the above product, and guaranteeing of clinic medicine effects of the above medicine.

The invention relates to a detecting method for traditional Chinese medicine components, in particular to a detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis, which is used for detecting effective components of Hugu capsules by means of multi-wavelength high-performance liquid chromatography. The detecting method includes the specific steps: a, preparing sample solution to be detected; b, preparing mixed reference sample solution; c, respectively detecting the sample solution to be detected and the mixed reference sample solution by means of high performance liquid chromatography; and d, comparing a high performance liquid chromatogram of the sample solution to be detected with that of the mixed reference sample solution. The detecting method can be used for simultaneously detecting chlorogenic acid, 2, 3, 5, 4'-tetrahydrostibene-2-O-beta-D-glucoside, ferulic acid, naringin and icariin and the content of the five effective components in the Hugu capsules. When the effective components in the Hugu capsules are detected by the method, high performance liquid chromatograms of standard substances of the five components are omitted, time and reagents can be reduced, and the detecting method is simple, convenient, high in precision and fine in reproducibility.

The invention provides a method for detecting a lung-heat clearing and cough relieving preparation. Raw materials of the lung-heat clearing and cough relieving preparation include phyllanthus emblica, costustoot and pterocephalus hookeri. The method is characterized in that the phyllanthus emblica, the costustoot and / or the pterocephalus hookeri in the lung-heat clearing and cough relieving preparation are identified by thin-layer chromatography. The method has the advantages of reproducibility, fine stability, simplicity and convenience in operational approach, high precision and specificity, capability of clearly developing spots, good resolution and the like, and meets the principle of accuracy, simplicity, convenience, sensitivity and quickness. By the aid of the established reliable quality detection method with high specificity, the quality of the lung-heat clearing and cough relieving preparation can be effectively controlled and is stable, safe and controllable.

The invention provides a two-dimension test method of the single-beam electron speckle interference in the symmetry distorted field. The process: (1) it tests the symmetry distorted object by the single-beam electron speckle interference system to get the phase position field of the object distortion by the phase displacement or the carrier wave modulate and the Fourier method; (2) it gets the second phase position figure by the image invert and the inverse computation to the distorted phase position figure, then to separate the in-face and off-face phase displacement field by undoing envelope, replacing the figure, overlapping and computing the algebra to get the component value of the object two-dimension distorted field. The invention has the simple system and provides a quick and stable test method for measuring the two dimensions.

The present invention relates to a breast cancer early warning chip for measuring I type theymine deoxyriboside kinase gene protein (hTK1) easily and rapidly. The invention is characterized in that the breast cancer early warning chip is composed of the following components: a strip fiber chromatography solid phase carrier NC film which is encapsulated with an anti-hTK1 monoclone antibody (numberfor 2A6-McAb) that is expressed and prepared by eukaryon at one end and is encapsulated with rabbit anti-mouse IgG monoclone antibody at the other end, a filtering sample paper, a water-absorbing glass fiber paper, and a polyester diaphragm which is marked with colloidal gold and secondary antibody hTK1 monoclone antibody (number for 3E8-McAb) bonder and the like components which are adhibited ona white PVC plastic piece. The invention has the characteristics and advantages of trace quantity, high speed, sensibility, accuracy, easiness, good stability, high repeatability, strong specificity,without special equipment or device, low detecting cost, measurement to single share or a plurality shares of specimen respectively, convenient carrying and convenience for site or field general investigation or physical examination.