119results about How to "The detection method is stable" patented technology

The invention provides a bovine tuberculosis antigen specific gamma-interferon enzyme-linked immuno sorbent assay (ELISA) kit. The kit comprises a bovine gamma-interferon monoclonal antibody coated ELISA plate, enzyme-labeled bovine gamma-interferon monoclonal antibodies, and protein solution containing mycobacterium bovis MPB83 and MPB70 and mycobacterium tuberculosis ESAT6 and CFP10. The antibodies are captured and detected for different antigen surfaces of gamma-interferon respectively. A bovine tuberculosis antigen specific gamma-interferon ELISA detection method established by using the mycobacterium bovis recombinant proteins MPB83 and MPB70 and mycobacterium tuberculosis recombinant proteins ESAT6 and CFP10 is relatively stable; the specificity of the method is 96 percent, and the sensitivity of the method is 88.6 percent; and the specificity and the sensitivity of the gamma-interferon ELISA detection method for the bovine tuberculosis are greatly improved.

The invention belongs to the technical fields of application methodology and electronic tongue detection, and discloses a difference degree calculation method and tea quality identification method based on the electronic tongue detection. The difference degree calculation method comprises the steps of electronic tongue detection, PCA analysis, and difference degree calculation; wherein the difference degree calculation adopts a recently improved calculation mode, each coordinate in the PCA score matrix is taken into account during the calculation process in a form of distance between each coordinate and the center point so as to reduce the affect of the random errors during the experiment process, and an sample acceptable fluctuation range is formed at the same time. The tea quality identification is on the basis of the electronic tongue difference degree detection of tea samples, the same tea species is taken as the standard, the credibility interval is set according to the difference degree in each group, and thus established is a standard sample experiment measured value error range. If the measured value of a tea sample is in the credibility interval, the sample is qualified; and if the measured value of a tea sample is not in the credibility interval, the sample is disqualified. The method can be used to control tea quality, and is a more efficient and stable detection method for market, enterprises, and detection organizations.

The invention discloses a method for detecting fluidity of slag in a coal gasifier. The method comprises the following steps of: acquiring a sound wave signal; preprocessing the sound wave signal; extracting feature parameters of the sound wave signal; building a prediction model; and detecting the fluidity of the slag. The method for detecting the fluidity of the slag in the coal gasifier by combining an acoustic emission sensor or an acceleration sensor and a wave guiding rod has the characteristics of stability, safety, environmental friendliness and the like, and is applicable to on-line detection of an industrial production process; a sound wave receiving device array is adopted, and measurement accuracy is improved by multi-sensor data fusion; and on-line detection of the fluidity of the slag is realized by a slag fluidity index number detecting technology based on sound wave detection. Compared with the prior art, the method is sensitive and is high in detection accuracy; and early warning of slag blockage is realized by a slag blockage early warning and control technology based on slag fluidity index number detection, the viscosity of the slag can be controlled within a range above or below an objective value by 5%, and the slag blockage is effectively avoided.

The invention provides a method for extracting and detecting a biotin of a bird nest. The method comprises the following steps of: extracting the biotin from the bird nest by using dimethylformamide; performing centrifugal purification and concentration to prepare a sample solution; by using a one-point external standard method, developing the sample solution and a biotin standard solution on a high-performance thin-layer plate coated with silica gel 60F254 by using a developer, wherein the developer is a mixture of chloroform, acetone, methanol and glacial acetic acid; spraying 0.05 percent of potassium permanganate solution to a developed plate for coloring; and drying the developed plate, and then performing thin layer scanning with absorption wavelength of 400 nm to obtain the contentof the biotin in the bird nest. The method has the advantages of simplicity, accuracy, quickness, stability and reliability, and the defects of complicated detection steps and high time consumption of the conventional microbiological method, and high investment cost, large solvent dosage and high cost of a high performance liquid chromatography are overcome.

The present invention relates to a breast cancer early warning chip for measuring I type theymine deoxyriboside kinase gene protein (hTK1) easily and rapidly. The invention is characterized in that the breast cancer early warning chip is composed of the following components: a strip fiber chromatography solid phase carrier NC film which is encapsulated with an anti-hTK1 monoclone antibody (number for 2A6-McAb) that is expressed and prepared by eukaryon at one end and is encapsulated with rabbit anti-mouse IgG monoclone antibody at the other end, a filtering sample paper, a water-absorbing glass fiber paper, and a polyester diaphragm which is marked with colloidal gold and secondary antibody hTK1 monoclone antibody (number for 3E8-McAb) bonder and the like components which are adhibited on a white PVC plastic piece. The invention has the characteristics and advantages of trace quantity, high speed, sensibility, accuracy, easiness, good stability, high repeatability, strong specificity, without special equipment or device, low detecting cost, measurement to single share or a plurality shares of specimen respectively, convenient carrying and convenience for site or field general investigation or physical examination.

The invention discloses a protein chip used for detecting human ferrum reserve, which belongs to the field of human ferrum nutrition detection. The protein chip comprises a solid-phase carrier, antiserum ferritin monoclonal antibodies, antiserum transferrin receptor monoclonal antibodies and anti-C-reactive-protein monoclonal antibodies, wherein the antiserum ferritin monoclonal antibodies, the antiserum transferring receptor monoclonal antibodies and the anti-C-reactive-protein monoclonal antibodies are distributed on the solid-phase carrier in an array manner, wherein the monoclonal antibodies are fixed on the surface of the solid-phase carrier by covalent bonds, and the solid-phase carrier is finished by hydroformylation. The invention further discloses a detection reagent kit based on the protein chip. Compared with the prior art, the protein chip can detect three indexes of human ferrum reserve simultaneously, is accurate and reliable in results, high in detection specificity, sensitivity and detection speed and stable in detection results. Furthermore, by multi-sample microarray technology, the protein chip is applicable to screening and detecting of massive people, low in blood sample quantity, detection and cost and low in harms to examined people.

The invention discloses a method for extracting biotin from Antarctic krill and a high-efficiency thin layer scanning analysis method. The method comprises the steps of: extracting biotin from Antarctic krill by using dimethyl formamide; centrifuging, removing impurities and concentrating to prepare a sample solution; spreading the biotin standard sample solution on a high-efficiency thin-layer plate coated with silica gel GF254 by adopting an external standard one point method, wherein a spreading agent is a mixed solution prepared from methylene dichloride, isopropanol, methanol and glacial acetic acid in proportion; spraying for dying the spread spradig plate by adopting a dying solution; putting the plate into a fume cupboard, and after five minutes, spraying a 10% of paraffin-chloroform solution; and carrying out thin-layer scanning with an absorption wavelength of 530nm, and calculating the content of the biotin in the Antarctic krill. The method has the advantages of simplicity, accuracy, rapidness, reliability and stability.

The invention discloses a detection method for a Cinnamomum migao heart-comforting preparation. The Cinnamomum migao heart-comforting preparation is prepared from the following drugs by weight: 3500-6000 parts of Cinnamomum migao, 40-150 parts of borneol, 2500-5000 parts of Ligusticum wallichii, and 250-500 parts of Allium macrostemon. The method provided in the invention takes a ferulic acid reference sample for reference, and adopts methanol-acetonitrile-0.1% phosphoric acid as a mobile phase to determine the content of Ligusticum wallichii in the product, with the methanol, the acetonitrile and the 0.1% phosphoric acid being in a volume ratio of 15:10:75. Compared with the prior art, the method has the characteristics of specificity, feasibility and good reproducibility, and can effectively control the quality of the Cinnamomum migao heart-comforting dropping pill preparation. Repeated tests confirm that the detection method has the advantages of stability, simplicity, accurate result as well as good reproducibility, so that the method can be used as a detection method for Cinnamomum migao heart-comforting dropping pills, oral liquids, syrups, granules, freeze-dried powder injections, and can be taken as an index for inspecting the technological stability.

The invention provides a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) fingerprint detection method for a main saponin component of a compound wintercreeper preparation (including mixture, capsules and tablets). The fingerprint detection method of the invention comprises the steps of: preparing test solution and measuring the fingerprint under a certain chromatographic condition. Scientific tests prove that the fingerprint measured by the method has high repeatability, so that the method can be used as a method for checking and identifying the main saponin component of the compound wintercreeper preparation and can also be used for measuring astragaloside content and ginsenoside Rb1 content of the preparation.

The invention provides a traditional Chinese medicine composition of a bone healing medicine, a preparing method thereof and a detecting method thereof. Each preparation unit of the traditional Chinese medicine composition contains pseudo-ginseng which is no less than 2.2mg by total content of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 and / or Carthamus tinctorius which is no less than 0.03mg by content of hydroxysafflor yellow A, quality is more stable, and curative effect is more exact. According to the detecting method, the content of the notoginsenoside R1, the ginsenoside Rg1, the ginsenoside Rb1 and / or the content of the hydroxysafflor yellow A are detected through detecting methods including a high-performance liquid chromatography method. The method is simple and stable, high in accuracy and good in reproducibility, and capable of representing effective ingredient content of the traditional Chinese medicine composition of the bone healing medicine completely and effectively and benefiting monitoring product quality.

The invention provides a compound detection reagent for detecting a sulfonamide compound and a detection method thereof. The compound detection reagent is prepared from an organic solvent solution containing various sulfonamide compound reference substances and a protein precipitator. The compound detection reagent provided by the invention can be used for simultaneously analyzing and detecting 28 sulfonamide compounds; the detection scope is wide; the scheme of protein precipitation or liquid-liquid extraction is adopted in the process of sample treatment, so that 28 sulfonamide compounds in the biological sample are purified and enriched; the detection method adopting the compound detection reagent provided by the invention has the advantages of simplicity, accuracy, quickness, stability and less sample capacity; the complex steps of derivatization and enzymatic conversion are not required; the chemical reagent used in the pre-treating process is less; the detection method is environment-friendly; the detection result is not interfered by endogenous substances; the false negative / positive rate is low; the cost of the reagent is low; the compound detection reagent is fit for quick analysis for a large amount of samples.

The invention provides a detection method of a Wuweiganlu preparation. The method comprises the following steps of: performing microscopic identification of the microscopic characteristics of juniperus formosana and myricaria in the Wuweiganlu preparation; performing thin-layer chromatography identification of rhododendron anthopogonoide, juniperus formosana and artemisia sieversiana in the Wuweiganlu preparation; and measuring the content of ephedrine hydrochloride and pseudoephedrine hydrochloride in ephedra and the content of artemisetin in artemisia sieversiana in the preparation by a high performance liquid chromatography. The detection method provided by the invention has the advantages of good reproducibility and stability, high precision, strong specificity, clear spot color, high separation degree, accurate content and the like, and is simple to operate; and by creating a reliable quality detection method with strong specificity, the quality of the Wuweiganlu preparation can be effectively controlled so that the quality of the Wuweiganlu preparation is stable, safe and controllable.

The invention discloses a detection method of a nine-component heart-calming particle. The detection method of the nine-component heart-calming particle can carry out thin-layer chromatography on spina date seed, schisandra chinensis, corydalis tuber, cinnamon, asparagus fern and polygala tenuifolia in the nine-component heart-calming particle, microscopic identification on calcium oxalate in ginseng and determine HPLC (high performance liquid chromatography) content of ginsenoside Rg1 and ginsenoside Re. The detection method of the nine-component heart-calming particle has the characteristics of stability, high precision, good reproducibility, high recovery ratio and good separation effect. By adopting the detection method of the nine-component heart-calming particle, the quality of the nine-component heart-calming particle can be further comprehensively controlled, the provided method for determining the content of ginsenoside Rg1 and ginsenoside Re can realize good baseline separation effect on ginsenoside Rg1 and ginsenoside Re, and a determination result is accurate and the reproducibility is good.

The invention discloses an indirect ELISA assay kit for detecting a novel duck reovirus antibody. The kit contains an ELISA plate coated with a novel duck reovirus sigmaC protein recombinant antigen.A preparation method of the novel duck reovirus sigmaC protein recombinant antigen comprises the following steps: taking a complete genome sequence of the duck reovirus with the preservation number ofCCTCC NO:V201818 as a template; amplifying by using a primer pair to obtain sigmaC gene segments; constructing a recombinant expression vector; expressing a duck sigmaC protein recombinant antigen bya prokaryotic expression system. The indirect ELISA assay kit disclosed by the invention can quickly and effectively detect the novel duck reovirus antibody which is newly broken out and takes tarsaljoint swelling as a main symptom so as to monitor epidemic conditions of the novel duck reovirus in a duck plump.

The invention discloses a method for extracting oestrone from a euphausia superba and an efficient thin layer chromatography scanning and detecting method. The method for extracting the oestrone from the euphausia superba comprises the following steps: extracting the euphausia superba as a raw material through methanol; and filtering, and steaming a filtrate to dry through a rotary evaporation instrument, so as to obtain a crude extract containing the oestrone. The oestrone of the euphausia superba is detected by using an efficient thin layer chromatography scanning method. A developing solvent is a mixture with a volume ratio of carbon tetrachloride to ethyl acetate to acetone to glacial acetic acid to methylbenzene to cyclohexane of (78-80) to (9-11) to (9-11) to (2-4) to 3d to 2d; a coloring agent is a 5% ferric chloride water solution; the content of the acetone can be obtained through calculation of absorbances of a detection sample product and a standard product by carrying out scanning detection with an absorption wavelength of 600nm by using a CAMAG thin layer chromatography scanner III. The method has the advantages of being simple to operate, fast, accurate and reliable.

The invention discloses a detection method of Yinqiao powder. The detection method can simultaneously determine the content of chlorogenic acid, forsythin, forsythiaside A and arctiin. The detection method has the advantages of high efficiency, fastness, high accuracy, high sensitivity, good repeatability, reliable result, effective control of the quality of the above product, and guaranteeing of clinic medicine effects of the above medicine.

The invention relates to a detecting method for traditional Chinese medicine components, in particular to a detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis, which is used for detecting effective components of Hugu capsules by means of multi-wavelength high-performance liquid chromatography. The detecting method includes the specific steps: a, preparing sample solution to be detected; b, preparing mixed reference sample solution; c, respectively detecting the sample solution to be detected and the mixed reference sample solution by means of high performance liquid chromatography; and d, comparing a high performance liquid chromatogram of the sample solution to be detected with that of the mixed reference sample solution. The detecting method can be used for simultaneously detecting chlorogenic acid, 2, 3, 5, 4'-tetrahydrostibene-2-O-beta-D-glucoside, ferulic acid, naringin and icariin and the content of the five effective components in the Hugu capsules. When the effective components in the Hugu capsules are detected by the method, high performance liquid chromatograms of standard substances of the five components are omitted, time and reagents can be reduced, and the detecting method is simple, convenient, high in precision and fine in reproducibility.

The invention provides a method for detecting a lung-heat clearing and cough relieving preparation. Raw materials of the lung-heat clearing and cough relieving preparation include phyllanthus emblica, costustoot and pterocephalus hookeri. The method is characterized in that the phyllanthus emblica, the costustoot and / or the pterocephalus hookeri in the lung-heat clearing and cough relieving preparation are identified by thin-layer chromatography. The method has the advantages of reproducibility, fine stability, simplicity and convenience in operational approach, high precision and specificity, capability of clearly developing spots, good resolution and the like, and meets the principle of accuracy, simplicity, convenience, sensitivity and quickness. By the aid of the established reliable quality detection method with high specificity, the quality of the lung-heat clearing and cough relieving preparation can be effectively controlled and is stable, safe and controllable.

The invention relates to a non-phosphorus corrosion and scale inhibitor suitable for medium-and-low-hardness and strong-corrosion water and application of the non-phosphorus corrosion and scale inhibitor. The non-phosphorus corrosion and scale inhibitor is prepared from the following ingredients by weight percent: 15-30% of an AMPS-containing terpolymer, 12-20% of hydrolyzed polymaleic acid, 0.1-10% of sodium gluconate, 15-40% of a fatty alcohol polyoxyalkylene alkyl ether-carboxyl-sulfopropionate copolymer, 0.1-3% of a pH regulator, 0.1-12% of zinc salt, 0.1-5.0% of benzotriazole, 0.5-5.0% of a water-soluble inert fluorescent tracer and 15-40% of deionized water. Compared with the prior art, the non-phosphorus corrosion and scale inhibitor provided by the invention solves the problem that a common non-phosphorus corrosion and scale inhibitor cannot draw the corrosion and scale inhibition function into consideration during concentration of a circulating water system. The non-phosphorus corrosion and scale inhibitor is favorable in performance and good in stability, has general applicability to medium-and-low-hardness and strong-corrosion water, and has the characteristics of no phosphorus, environmental friendliness and high efficiency.

The invention discloses a method for extracting biotin from corn steep liquor. The method is characterized by using corn steep liquor as a raw material, carrying out extraction with N,N-dimethylformamide, carrying out extraction and impurity removal with absolute ethyl alcohol, carrying out extraction and impurity removal with acetone and carrying out extraction and impurity removal with butyl acetate and cyclohexane, thus finally obtaining a sample solution containing biotin. The invention also provides a thin layer chromatography (TLC) scanning detection method of biotin. The detection method comprises the following steps: (1) dropping the sample solution to be detected on an efficient thin layer plate coated with silica gel GF254, adding a developer, developing the sample solution to be detected in a developing cylinder, and then taking out the thin layer plate and putting the thin layer plate in a fume hood to be aired; (2) spray-dyeing the developed and aired plate with a dyeing solution to obtain spots, in obvious contrast to the background, of standard substances and samples; (3) carrying out TLC scanning by utilizing a Camag3 type thin layer scanner in the absorption wavelength of 530nm, and computing the mass concentration of biotin in the sample solution. The methods have the advantages of simplicity, quickness, accuracy, reliability, stability and the like.

The invention provides a two-dimension test method of the single-beam electron speckle interference in the symmetry distorted field. The process: (1) it tests the symmetry distorted object by the single-beam electron speckle interference system to get the phase position field of the object distortion by the phase displacement or the carrier wave modulate and the Fourier method; (2) it gets the second phase position figure by the image invert and the inverse computation to the distorted phase position figure, then to separate the in-face and off-face phase displacement field by undoing envelope, replacing the figure, overlapping and computing the algebra to get the component value of the object two-dimension distorted field. The invention has the simple system and provides a quick and stable test method for measuring the two dimensions.

The invention relates to a method for detecting total ginkgolic acid in ginkgo leaf extract. The method comprises the following steps: preparing a test solution; preparing a reference solution; separately detecting the test solution and the reference solution by using high performance liquid chromatography so as to obtain chromatograms respectively; and calculating the content of total ginkgolic acid in the to-be-detected ginkgo leaf extract by using an external standard method according to the area of total ginkgolic acid in the reference chromatogram and the area of total ginkgolic acid in the test chromatogram. According to the invention, a method for preparing a sample solution is simple and convenient to operate; and the detection method is stable, uniform, controllable and convenient, and can quickly and accurately reflect the real situation of the to-be-detected ginkgo leaf extract.

The present invention relates to a breast cancer early warning chip for measuring I type theymine deoxyriboside kinase gene protein (hTK1) easily and rapidly. The invention is characterized in that the breast cancer early warning chip is composed of the following components: a strip fiber chromatography solid phase carrier NC film which is encapsulated with an anti-hTK1 monoclone antibody (numberfor 2A6-McAb) that is expressed and prepared by eukaryon at one end and is encapsulated with rabbit anti-mouse IgG monoclone antibody at the other end, a filtering sample paper, a water-absorbing glass fiber paper, and a polyester diaphragm which is marked with colloidal gold and secondary antibody hTK1 monoclone antibody (number for 3E8-McAb) bonder and the like components which are adhibited ona white PVC plastic piece. The invention has the characteristics and advantages of trace quantity, high speed, sensibility, accuracy, easiness, good stability, high repeatability, strong specificity,without special equipment or device, low detecting cost, measurement to single share or a plurality shares of specimen respectively, convenient carrying and convenience for site or field general investigation or physical examination.

The invention discloses an indirect ELISA detection kit for detecting a novel goose astrovirus antibody. The kit includes an ELISA plate coated with a novel goose astrovirus ORF1b protein recombinantantigen. The preparation method of the novel goose astrovirus ORF1b protein recombinant antigen includes: taking the complete gene sequence of goose astrovirus with a preservation number of CCTCC NO:V201808 as the template, conducting primer pair amplification to obtain an ORF1b gene fragment, constructing a recombinant expression vector, and expressing the goose astrovirus ORF1b protein recombinant antigen by a prokaryotic expression system. The indirect ELISA detection kit provided by the invention can achieve rapid and effective detection of the new outbreak novel goose astrovirus antibodywith gout as the main symptom, thus monitoring the epidemic situation of novel goose astrovirus in a gaggle of geese.

The invention discloses an indirect ELISA detection kit of duck reovirus causing the spleen necrosis. The kit comprises an elisa plate coated with novel duck reovirus sigma C protein recombinant antigen; a preparation method of the novel duck reovirus sigma C protein recombinant antigen comprises the following steps: adopting a complete gene sequence of the duck reovirus with a preservation numberof CCTCC NO: V201843 as a template, performing the amplification by using a primer pair to obtain a sigma C gene fragment, establishing a recombinant expression vector, and expressing the duck reovirus sigma C protein recombinant antigen by virtue of a prokaryotic expression system. The indirect ELISA detection kit can rapidly and effectively detect the outbreak novel duck reovirus antibody withthe main symptom of hock swelling so as to monitor the prevalence state of the novel duck reovirus in ducks.

The invention discloses an extraction and thin layer chromatographic scanning detection method for defatted Euphausia superb saponin. The method specifically comprises the following main steps: extracting defatted Euphausia superb saponin and performing thin layer chromatographic scanning detection. According to the extraction method and thin layer chromatographic scanning detection method for defatted Euphausia superb saponin provided by the invention, the detection method is simple, rapid, accurate, reliable and stable.

The present invention relates to a detection method for related substances in a memantine hydrochloride preparation, wherein particularly a gas chromatography method is adopted to detect the related substances. Memantine hydrochloride has a stable structure and does not have chromophore, such that memantine hydrochloride is difficultly detected by using an ultraviolet detector. According to the present invention, the disadvantage of memantine hydrochloride is overcome, and defects of poor separation capability of the TLC method and emission of a lot of harmful waste gas of the evaporative light-scattering method are overcome; and the detection method has characteristics of accuracy, convenient operation, good reproducibility and high sensitivity, can completely meet requirements of determination on the related substance impurities, can be provided for controlling special impurities in samples and ensuring product quality, and is suitable for industrial production application.

The invention discloses a bioactivity detection method and application of recombined humanized neuroglobin. The detection method disclosed by the invention comprises the following steps: 1) preparing the recombined humanized neuroglobin to be detected into a sample solution of the set concentration; 2) detecting the capability of the sample solution in eliminating radicals, and using the superoxide anion clearance rate through rhNgb, the hydrogen oxide clearance amount through rhNgb and / or the hydroxyl radical clearance rate through rhNgb as detection indexes; and 3) computing the detection data and comparing the detection data with the bioactivity standard data of the recombined humanized neuroglobin to obtain the detection result. The invention also provides the application of the neuroglobin Ngb in the preparation of a medicament with a function of eliminating the radicals, which provides the train of thoughts for the development of a new medicament. The detection method disclosed by the invention is stable, has the advantages of reliable detection result, high repeatability of the detection data and the like and can be widely applied to tracking the activity of the rhNgb and biological products containing rhNgb, and the detection result can better reflect the bioactivity of rhNgb proteins.

The invention discloses a method for extracting biotin with molasses as a raw material. The method is characterized by using molasses as a raw material, adding ethanol, carrying out 40KHz ultrasonic treatment to obtain molasses clinkers, adding ethanol, carrying out 20KHz ultrasonic treatment and carrying out extraction with N,N-dimethylformamide in a cooperative manner, thus finally obtaining a sample solution containing biotin. The invention also provides a thin layer chromatography (TLC) scanning detection method of biotin. The detection method comprises the following steps: (1) dropping the sample solution to be detected on a silica gel plate, adding a developer, developing the sample solution to be detected in a developing cylinder, and then taking out the thin layer plate and putting the thin layer plate in a fume hood to be aired; (2) spray-dyeing the developed and aired plate with a dyeing solution to obtain spots, in obvious contrast to the background, of standard substances and samples; (3) carrying out TLC scanning by utilizing a thin layer scanner in the absorption wavelength of 530nm, and computing the mass concentration of biotin in the sample solution. The methods have the advantages of simplicity, quickness, accuracy, reliability, stability and the like.

The invention relates to a method of in-situ assembly, electrochemical reduction and oxidation state conversion representation of graphene oxide, belonging to the technical field of nano materials. According to the method, a surface plasmon resonance technology is used to in situ monitoring the assembly process of chemically prepared graphene oxide on the surface of a gold film and in situ quantitatively detecting different reduction degrees of graphene oxide. The method comprises the following steps: assembling graphene oxide on the surface of the gold film through physical absorption; in situ reducing graphene oxide into graphene by using an electrochemical method; and fitting a curve of a surface plasmon spectrum by using software to detect the assembly and reduction degrees of graphene oxide on the surface of a sensor chip. The method has the advantages that the assembly, electrochemical reduction and detection of graphene oxide are implemented on one set of machine, instruments and equipment are low in price and simple to operate, and the efficiency and the accuracy are high.