Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Breast cancer early warning chip for easily rapid measuring human I type thymidine kinase gene protein

A thymidine kinase and gene technology, which is applied in the field of breast cancer early warning chips, can solve the problems of easy missed detection in early stage patients, high technical difficulty, cumbersome operation steps, etc.

Inactive Publication Date: 2012-01-04
丁克祥
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the sensitivity of any single application of breast cancer-related tumor markers found so far is low, and the positive rates of both stages I and II are less than 30%, and early-stage patients are easy to be missed.
⑧Direct DNA sequence determination, denaturing gradient gel electrophoresis, heteroduplex analysis, fluorescent label mismatch analysis and protein truncation analysis, real-time fluorescent PCR technology, single-strand conformation polymorphism analysis, multiple heteroduplex analysis And sequencing and fluorescent double-stranded probe methods are mostly protected by patents, and because some methods are technically difficult, require high equipment, cumbersome operation steps, poor specificity, high cost, and easy pollution to cause false positives, general hospital laboratories are very difficult Difficult to apply and not suitable for large-scale screening
[0010] Even if some of the most modern and advanced diagnostic methods and detection technologies are used at present, it can provide a certain basis for the early diagnosis of clinical breast cancer, but because these diagnostic methods and detection technologies require special conditions, they need to be carried out in some large general hospitals, and , most of these diagnostic methods and detection technologies are professional detection reagents, complex measurement methods, various detection instruments, cumbersome operation steps, special laboratory requirements, time-consuming and labor-intensive, and expensive determination, which makes it difficult for many people, especially some high-risk groups. Go to the hospital frequently and in time for examination or census. Therefore, many good opportunities for early diagnosis and early detection of breast cancer have been missed, resulting in lifelong regret and pain.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Breast cancer early warning chip for easily rapid measuring human I type thymidine kinase gene protein
  • Breast cancer early warning chip for easily rapid measuring human I type thymidine kinase gene protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0052] ① Cloning and sequence analysis of hTK1 gene:

[0053] 1) Reverse transcription PCR amplification of TK1cDNA: Extract RNA from 5 million Hela cells with Trizol reagent kit that can extract total ribonucleic acid (RNA) from animal cells and tissues, bacteria, fungi, etc., and treat with methotrexate for 48 hours , and after purification, a highly efficient alkylating agent-diethyl pyrocarbonate (DEPC) was used to dissolve the precipitate in water to obtain 30 μl. Since DEPC can destroy ribonuclease (RNase) activity, it can remove RNase contamination.

[0054] When performing reverse transcription, add 5-fold buffer: 4 μl, primer Oligo-dT (0.5 μg / 1 μl) 1 μl, deoxynucleoside triphosphate (dNTP) at 10 mMol / L concentration: 2 μl, RNA: 10 μl, deionized water : 2 μl, mixed thoroughly, and placed in a constant temperature water bath at 70°C for 10 minutes; then, placed on ice for 2 minutes; added 1 μl reverse transcriptase (50 U, Roche), mixed well, and placed in a water bath ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Sensitivityaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a breast cancer early warning chip for measuring I type theymine deoxyriboside kinase gene protein (hTK1) easily and rapidly. The invention is characterized in that the breast cancer early warning chip is composed of the following components: a strip fiber chromatography solid phase carrier NC film which is encapsulated with an anti-hTK1 monoclone antibody (numberfor 2A6-McAb) that is expressed and prepared by eukaryon at one end and is encapsulated with rabbit anti-mouse IgG monoclone antibody at the other end, a filtering sample paper, a water-absorbing glass fiber paper, and a polyester diaphragm which is marked with colloidal gold and secondary antibody hTK1 monoclone antibody (number for 3E8-McAb) bonder and the like components which are adhibited ona white PVC plastic piece. The invention has the characteristics and advantages of trace quantity, high speed, sensibility, accuracy, easiness, good stability, high repeatability, strong specificity,without special equipment or device, low detecting cost, measurement to single share or a plurality shares of specimen respectively, convenient carrying and convenience for site or field general investigation or physical examination.

Description

technical field [0001] The invention relates to a breast cancer early-warning chip for the simple and rapid detection of type I thymidine kinase gene protein (hTK1), which is developed by cross-scientific technologies such as genetic engineering methods and molecular immunology techniques, and is mainly used for early warning of the risk of occurrence or development of female breast cancer At the same time, it can also be used as a new health care method for women's health protection reminder and clinical diagnosis of early breast cancer. Background technique [0002] Background on breast cancer and its diagnostic technology: Breast cancer is a common and frequently occurring malignant tumor in humans. It is a malignant tumor of epithelial origin and a systemic complex disease. The most common early clinical signs and manifestations mainly include painless lumps in the breast and armpits, changes in breast shape or size, inverted nipples, nipple discharge, "dimple-like" depr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/532G01N33/577
Inventor 丁克祥刘卫国费定宇刘莉丁宇
Owner 丁克祥
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products