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49 results about "Diethyl pyrocarbonate" patented technology

Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues.

Quantitative detection kit, detection method, primer and probe for hepatitis B virus nucleic acid

The invention discloses a quantitative detection kit, detection method, primer and probe for hepatitis B virus nucleic acid, belonging to the technical field of biology. The invention relates to a gene detection technology of a virus causing various types of acute, chronic and severe hepatitis of human beings, which is suitable for qualitative and quantitative detection of the hepatitis B virus. The kit comprises PCR (Polymerase Chain Reaction) reaction liquid, wherein the reaction liquid comprises DEPC (Diethyl Pyrocarbonate) treating water, Taq enzyme, dNTPs (Deoxynucleotide Triphosphates),10*PCR Buffer, a solution containing Mg<2+> ions, a hepatitis B virus positive primer: 5'-TTGTCCTGGYTATCGYTGGAT-3', a hepatitis B virus negative primer: 5'-TGAGGCATAGCAGCAGGATGA-3', a hepatitis B virus probe: 5'-CTGCGGCGTTTTAT-3'; and the kit also comprises a DNA (Deoxyribonucleic Acid) extracting solution, a negative control material, a working standard product, a positive control material and acritical positive control material. The hepatitis B virus nucleic acid is detected quantificationally by adopting a real-time fluorescent quantitative PCR technology. The invention has the characteristics of specificity, sensitivity, quickness and easiness and convenience for operation.
Owner:WUHAN BIOTECH GENE ENG

Kit for diagnosing reovirus genes of grass carps and application thereof

The invention relates to a kit for diagnosing the reovirus genes of grass carps, comprising an RNA (Ribonucleic Acid) extraction reagent for viruses in visceral tissues, an RAN extraction reagent for cell infecting viruses, an RT (Reverse Transcriptase) reaction reagent and a PCR (Polymerase Chain Reaction) reaction reagent, wherein the RNA extraction reagent for the viruses in the visceral tissues comprises the components of DEPC (Diethyl Pyrocarbonate) water, protease K (1 mg/mL, pH is 8.0), SDS (Sodium Dodecyl Sulfate) (1%), phenol/chloroform/isoamylol (25/24/1), chloroform/isoamylol (24/1), isopropanol and 70% ethanol; the RAN extraction reagent for the cell infecting viruses comprises the components of Trizol, chloroform, isopropanol, 70% ethanol and the DEPC water; the RT reaction reagent comprises the components of 5*AMV Buffer, dNTP, the DEPC water, a reverse primer R1, an RNA enzyme inhibitor RNAsin (R1) and a reverse transcriptase AMV; and the PCR reaction reagent comprises the components of 10*PCR buffer (containing Mg<2+>) and DNTP Mixture (which are respectively 2.5 mmol/L), a specific oligonucleotide primer F1, the primer R1, the DEPC water and Taq E (5U/muL), the forward primer F1:5'-ATCCCGTATATCTATGGCTT-3', and the reverse primer R1:5'TTGGAGACGAACATAGACGC-3. The kit is used for diagnosing the reovirus genes of the grass carps.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES

Kit capable of quickly detecting classical swine fever virus/porcine reproductive and respiratory syndrome virus/pseudorabies virus/porcine parvovirus

The invention relates to a kit capable of quickly detecting classical swine fever virus / porcine reproductive and respiratory syndrome virus / pseudorabies virus / porcine parvovirus, in particular to a multiplex real-time fluorescence polymerase chain reaction technology capable of detecting CSFV (classical swine fever virus), PRRSV (porcine reproductive and respiratory syndrome virus), PRV (pseudorabies virus) and PPV (porcine parvovirus) simultaneously.The kit mainly comprises a RT-PCR (reverse transcription-polymerase chain reaction) mixture, primer probe mixed liquor, an RT-PCR enzyme system and DEPC (diethyl pyrocarbonate) H2O as well as packaging boxes for packaging reagent bottles or tubes separately and in a centralized manner.Through multiple fluorescence channels for separate detection, the kit applying a one-step real-time fluorescence PCR mode is capable of detecting and identifying the classical swine fever virus, the porcine reproductive and respiratory syndrome virus, the pseudorabies virus and the porcine parvovirus quickly and accurately and can be widely applied to multiple fields such as early clinical diagnosis of porcine reproductive disturbance diseases, port inspection and quarantine, plague prevention and scientific research.
Owner:DAAN GENE CO LTD

Reagent kit, method, primers and probe for quantitative detection of nucleic acid of swine fever virus

The invention discloses a reagent kit, a method, primers and a probe for quantitative detection of nucleic acid of a swine fever virus and belongs to the technical field of biology. The reagent kit comprises PCR (polymerase chain reaction) liquid including DEPC (diethyl pyrocarbonate) treating water, Taq enzyme, M-MLV reverse transcriptase, RNase inhibitor, dNTP Mix, 10X one-step RT-PCR (reverse transcription-polymerase chain reaction) Buffer, MgC12 solution, the forward primer of the swine fever virus, the reverse primer of the swine fever virus and the LNA (locked nucleic acid) probe of the swine fever virus, wherein the forward primer of the swine fever virus refers to: 5'-AACGGYAGTGCTTTCTAYC-3', the reverse primer of the swine fever virus refers to: 5'-GTGGRAAAGGCTTCTCTC-3', and the LNA probe of the swine fever virus refers to: 5'-accActTctGtyCtac-3'. The reagent kit further comprises RNA (ribonucleic acid) extracting solution, negative quality control serum, working standard serum, positive quality control serum and critical positive quality control serum. The real-time fluorescent quantitative PCR technology is used for quantitatively detecting the nucleic acid of the swine fever virus, and accordingly the reagent kit has the advantages of specificity, sensitivity, rapidness and simplicity and convenience to operate.
Owner:湖北万德瑞生命科学技术有限公司

Hepatitis B virus Adefovir dipivoxil drug-resistance nucleic acid quantitative detection reagent kit, detection method, primers and probes thereof

The invention discloses a hepatitis B virus Adefovir dipivoxil drug-resistant nucleic acid quantitative detection reagent kit, a detection method, primers and probes thereof. The reagent kit contains fluorescence quantitative PCR (Polymerase Chain Reaction) reaction liquid, including DEPC (Diethyl Pyrocarbonate) treated water, DNA (Deoxyribose Nucleic Acid) polymerases with the 5'->3' exonucleolytic activity, dNTPs (Deoxyribonucleotide Triphosphates), 10*fluorescence quantitative PCR Buffer, solution containing Mg2+ions, rt181 site forward primers, rt181 site reverse primers, rt181A site probes, rt181V site probes, rt181T site probes as well as rt236 site forward primers, rt236 site reverse primers, rt236N site probes and rt236 site probes peculiar to a rtN236T detection reagent kit; and DNA extracting solution, negative controls, working standards, positive controls and critical positive controls are also contained in the reagent kit. According to the detection method, the fluorescence quantitative PCR (Polymerase Chain Reaction) technology is adopted for quantitatively detecting hepatitis B virus Adefovir dipivoxil drug-resistant strains; according to the fluorescence quantitative PCR (Polymerase Chain Reaction) method, the step of PCR (Polymerase Chain Reaction) extension is avoided; and the probes can mark a variety of fluorescent marks; and meanwhile, the reagent kit has the advantages of specificity, sensitivity, quickness and convenience in operation.
Owner:WUHAN BIOTECH GENE ENG

Urine preservation solution, preservation method and urine preservation tube

The invention discloses a urine preservation solution which is an aqueous solution containing the following substances in percentage by mass: 0.02%-0.1% of an RNA (Ribonucleic Acid) enzyme inhibitor, 2%-10% of a DNA (Deoxyribose Nucleic Acid) enzyme inhibitor, 0.2%-0.8% of a nonionic surfactant, 1%-5% of a cell fixing agent, 1%-5% of a preservative and 1%-5% of an aldehyde group quencher, the RNA enzyme inhibitor at least comprises diethyl pyrocarbonate, and the DNA enzyme inhibitor at least comprises ethylenediamine tetraacetic acid. The invention further discloses a urine preservation method and a urine preservation tube. After urine is in vitro and is preserved by adopting the urine preservation solution provided by the invention, relatively accurate free DNA and RNA information can still be provided, the degradation of nucleic acid substances is inhibited, interference signals including but not limited to genome DNA and microorganism interference are inhibited, and an overall detection solution result still has relatively high sensitivity and relatively high accuracy. The operation is simple and the shelf life is long.
Owner:SANSURE BIOTECH INC

Reagent kit, method, primers and probe for quantitative detection of nucleic acid of swine fever virus

The invention discloses a reagent kit, a method, primers and a probe for quantitative detection of nucleic acid of a swine fever virus and belongs to the technical field of biology. The reagent kit comprises PCR (polymerase chain reaction) liquid including DEPC (diethyl pyrocarbonate) treating water, Taq enzyme, M-MLV reverse transcriptase, RNase inhibitor, dNTP Mix, 10X one-step RT-PCR (reverse transcription-polymerase chain reaction) Buffer, MgC12 solution, the forward primer of the swine fever virus, the reverse primer of the swine fever virus and the LNA (locked nucleic acid) probe of the swine fever virus, wherein the forward primer of the swine fever virus refers to: 5'-AACGGYAGTGCTTTCTAYC-3', the reverse primer of the swine fever virus refers to: 5'-GTGGRAAAGGCTTCTCTC-3', and the LNA probe of the swine fever virus refers to: 5'-accActTctGtyCtac-3'. The reagent kit further comprises RNA (ribonucleic acid) extracting solution, negative quality control serum, working standard serum, positive quality control serum and critical positive quality control serum. The real-time fluorescent quantitative PCR technology is used for quantitatively detecting the nucleic acid of the swine fever virus, and accordingly the reagent kit has the advantages of specificity, sensitivity, rapidness and simplicity and convenience to operate.
Owner:湖北万德瑞生命科学技术有限公司

Plant mRNA (messenger ribonucleic acid) extraction method

The invention discloses a plant mRNA (messenger ribonucleic acid) extraction method, belongs to the technical field of molecular biology, and particularly relates to the technical field of extracting mRNA from plant materials through acupuncture needles.The plant mRNA extraction method is provided to solve the problems of operation complexity, low efficiency and damage to plant materials in conventional mRNA extraction methods.The plant mRNA extraction method includes 1, treating an acupuncture needle; 2, putting the dried acupuncture needle into a mixed solution of trimethoxysilane, xylene and diisopropylethylamine for incubation; 3, embedding the acupuncture needle to enable poly-thymine to be adsorbed to the surface of the acupuncture needle; 4, autoclaving the embedded acupuncture needle subjected to ultrapure water elution; 5, punching the naturally dried acupuncture needle into a test position of a target gene of a tender plant material, keeping the acupuncture needle at the test position for 1-3 minutes, pulling out the acupuncture needle, and putting the acupuncture needle into a PCR (polymerase chain reaction) tube of diethyl pyrocarbonate water to complete plant mRNA extraction.The plant mRNA extraction method has the advantages that total RNA extraction is not needed, the mRNA can be extracted from the plant materials in 1-3 minutes, and simplicity, convenience and rapidness of the operation process are achieved.
Owner:NORTHEAST FORESTRY UNIVERSITY
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