171 results about "Isothiocyanic acid" patented technology

The present invention relates to a chemical reagent for detecting mercury ion in water and its preparation method. Said invention provides the structural formula of said reagent, and its preparation method includes the following steps: firstly, synthesizing rhodamine hydrazide, adding rhodamine dye in container, dissolving by using organic solvent, adding hydrazine hydrate, heating to make reflux reaction until the rhodamine dye colour is eliminated, filtering, washing the obtained solid by using water to obtain rhodamine hydrazide compound, adding organic solvent to dissolve said compound, adding aryl isothiocyanate, heating mixture and making reflux reaction, removing organic solvent, using column chromatography to make separation so as to obtain target product.

Yellow or oriental mustard seed can be fractionated to produce allyl isothiocyanate and p-hydroxybenzyl isothiocyanate, along with prepared mustard products, mustard protein and dietary fiber. Processes for the production of these various fractions from mustard seed stock are disclosed. The seed stock is comminuted with water to yield an activated slurry in which the enzyme myrosinase hydrolyses and deteriorates glycosinolates in the seed stock to isothiocyanates. Remaining slurry can then be sterilized and further conventionally processed to yield improved finished mustard products.

The invention discloses a lysis solution and an application thereof in tissue or cell preservation and RNA (Ribonucleic Acid) extraction. The lysis solution comprises a lysis solution A and a lysis solution B, wherein the lysis solution A comprises 2-4mol/L guanidinium isothiocyanate, 5-10mmol/L sodium chloride, 10-15mmol/L potassium chloride, the lysis solution B comprises 2-4mol/L guanidinium isothiocyanate, 1-5mmol/L spermidine, 30-40vt% of water saturated phenol, 0.4-0.5mol/L ammonium thiocyanate, 7-8vt% of glycerinum, 0.1-0.5mol/L sodium acetate and 0.2-0.5wt% of SDS (Sodium Dodecyl Sulfonate). When RNA is extracted by using the lysis solution A and the lysis solution B, tissue or cells do not need to be washed, and thus the extraction efficiency is remarkably improved; and due to synergistic effects of the components, the extracted RNA is high in purity and high in yield, and the purpose that animal RNA is extracted in laboratories rapidly with low cost and high quality in a large scale can be realized.

The invention provides a bifunctional fluorescent probe which is provided with the structure of the general formula I and adopts anthracene as the matrix. The preparation method of the bifunctional fluorescent probe comprises the following steps: conducting isocyanic acid esterification or isothiocyanic acid esterification on 9-anthracene methylamine; conducting the nucleophilic addition reaction to obtain the bifunctional fluorescent probe. The bifunctional fluorescent probe has the good fluorescence detection effect on iron ions; moreover, the system composed of the bifunctional fluorescent probe and the iron ions has the selective fluorescence enhanced detection effect on fluorine ions.

The invention discloses application of a PEI modified carbon dot-FITC compound as a rate fluorescent pH probe and a Cu2+ fluorescent probe. Cherry tomatoes are used as raw materials, carbon dots with fluorescence insensitive to pH changes are prepared through hydro-thermal synthesis, then CDs are modified with PEI, amidogen on PEI react with isothiocyanic acid radicals on FITC molecules, and the compound is formed. The responsivity of fluorescence of FITC in the compound to the pH changes is high, and a rate probe based on CDs and FITC fluorescence is used for pH detection; amidogen on PEI and Cu2+ are subjected to coordination, so that the fluorescence intensity of CDs and FITC is reduced along with increase of the concentration of Cu2+ in a solution, and Cu2+ detection is achieved. According to the constructed fluorescent probe, selected materials are novel, the advantages of being high in sensitivity, good in selectivity, easy to operate, wide in linear range, low in detecting limit and the like are achieved, and the constructed fluorescent probe can be used for efficient detection of the pH and Cu2+ in the aqueous solution and a biological sample.

The invention discloses a liquid crystal compound containing an isothiocyanic acid radical as well as a preparation method and an application thereof. The structural general formula of the liquid crystal compound containing the isothiocyanic acid radical is disclosed in a formula I. The compound containing the isothiocyanic acid radical is a liquid crystal monomer; the monomer of the class has bigger optical anisotropy (delta n); and after the monomer is added into mixed liquid crystal, the optical anisotropy of the mixed liquid crystal can be greatly increased so as to improve the property of the mixed liquid crystal. The formula I is as follows.

The invention discloses polymer nanoparticles capable of detecting hypochlorous acid and copper ions. The polymer nanoparticles are novel polymer nanoparticles prepared by taking a polyethylene glycol monomethyl ether chain transfer agent, styrene, 2-aminoethylmethacrylate hydrochloride, rhodamine B isothiocyanate and a 9,9-dioctyl polyfluorene-benzothiadiazole alternate copolymer as raw materials. The polymer nanoparticles can realize high-sensitivity ratio fluorescence detection on the hypochlorous acid and the copper ions in a pure water solution. Compared with an existing fluorescence detection technology, the prepared polymer nanoparticles have a double-ratio detection function and good biocompatibility; a synthetic route is simple and a detection method is simple and convenient; the polymer nanoparticles are applicable to amplified synthesis and actual production application, and have a great application prospect in the technical fields including analytical chemistry, life science, environmental science and the like.

The invention discloses an amphipathic fluorescent targeting nanometer micelle and preparing method, which comprises the following steps: polymerizing decomposable polyester and multi-ammino polymer to synthesize amphipathic drug carrier, adopting solvent evaporating method to prepare carrier micelle, packing the drug into hydrophobic or little water-soluble tumour prevention drug, grafting targeting molecular folacin on the micelle with ammino, grafting isothiocyanic acid fluorescein on the residual ammino.

A conjugate of human albumin (HA) and 2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (p-SCN-Bn-DOTA) insoluble in aqueous medium at a pH of 3-8.5 and whose IR spectrum shows an absorption at about 700 cm⁻¹ is described; a complex is further described consisting of a conjugate of HA and p-SCN-Bn-DOTA (HAC) according to claim 1 or 2 and a radioactive isotope of an element selected among the group consisting of P, Sr, Rh, Pd, I, Sm, Er, Au, At Bi, In, Lu, Y, Re, Cu and Ag, as well as procedures for preparing said conjugate and said complex as well as the use of such conjugate in the identification of mammalian neoplastic lesions by the R.O.L.L. methodology (“Radioguided Occult Lesion Localization”).

The invention discloses a new preparation method of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane, and particularly relates to a method for the extraction of sulforaphane from radish seeds or broccoli seeds or rape seeds. According to the method, air-dried radish seeds or broccoli seeds or rape seeds are used as a raw material for preparation of a radish seed crushing liquid, an enzyme deactivated broccoli seed or radish seed dispersion liquid, a degreased fine grinding liquid, a fermentation liquid, a protein removal fermentation liquid, a concentrated liquid, dry polysaccharide powder and high purity sulforaphane freeze-dried powder for preparation of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane with the purity of more than 98%. The production rate of the sulforaphane prepared by the method is high, the operation is simple, and the product has the characteristics of being high in product purity of more than 98%, green, non-pollution and the like. The product prepared by the method can be widely used in medicine and health care industry, and has cancer preventing and resistant function.

The invention relates to a collecting agent with an amide group and a thioamide group as well as a preparation method and application thereof. The collecting agent has both the amide group and the thioamide group and has stronger collecting capacity. The preparation method of the collecting agent comprises the following steps: carrying out a reflux reaction of ester containing an isothiocyano group or ester containing an isothiocyano group and an alkoxycarbonyl group, carbonate and amine containing an acetoacetyl group by taking ethanol as a solvent, removing the ethanol and adding hydrochloric acid, so as to obtain the collecting agent with the amide group and the thioamide group. The invention further provides the application of the collecting agent with the amide group and the thioamidegroup in flotation of metal sulfide minerals. The collecting agent in a structure with the amide group and the thioamide group has stronger collecting capacity for precious metal minerals such as chalcopyrites, galena, gold, silver and the like and also has good selectivity for minerals such as pyrites, sphalerites and the like, and the adaptive scope of the pH value of ore pulp is wide.

The present invention provides a method of treating an aqueous system for biocide control, by adding an acidified solution that includes monoalkyldithiocarbamate salt and alkyl isothiocyanate to an aqueous system. A biocide feed system is also provided that is configured to generate a reproducible level of alkyl isothiocyanate. The method and system provide effective levels of alkyl isothiocyanate for rapid biocidal activity, along with levels of monoalkyldithiocarbamate salt which can decompose to form additional alkyl isothiocyanate and provide longer-term biocidal activity.

The invention discloses an isotope labeling reagent which is an isothiocyanopyrimidine isotope labeling reagent with a chemical name of [d0]/[d6]-4,6-dimethoxine-2-isothiocyanate. The preparation method of the reagent comprises the following steps of: firstly, reacting 4,6-dichloro-2-amiopyrimidine with sodium methoxide/deuterosodium methoxide to obtain a [d0]/[d6]-4,6-dimethoxy-2-amiopyrimidine crude product; carrying out reflux reaction on the obtained crude product, thiophosgene and sodium bicarbonate to obtain a final crude product; and then carrying out column chromatography to obtain the isotope labeling reagent disclosed by the invention. The isotope labeling reagent disclosed by the invention can be applied to the identification analysis of polypeptide N-end residues and the quantitative analysis of protein, can speed up the labeling reaction rate and improve a signal of the labeled peptide section, is beneficial to strengthening the accuracy and the credibility of the identified peptide section and has the advantages of less molecular weight of the labelled part, relative simple structure, stable performance, simpleness and convenience for operation, and the like.

The invention discloses a naphthylthiourea-modified permethylated beta-cyclodextrin derivative, a preparation method and an application method thereof, the chemical formula of the compound is C73H118N2O34S, and the structure and the schematic diagram are shown as attached figures. The compound is simply prepared by the reaction of an amino derivative of cyclodextrin and naphthalene isothiocyanatewith high yield. The compound has high selectivity and high sensitivity on the recognition of mercury ions due to high sulphophile affinity of mercury. The divalent mercury ions are added in the compound in a water phase, fluorescence can be greatly ascended in a short period of time, the detection limit is 50ppb, the compound is resistant to the addition of other ions, such as, Li<+>, Na<+>, K<+>, Mg<2+>, Ca<2+>, Sr<2+>, Ba<2+>, Ag<+>, Cd<2+>, Ce<2+>, Co<2+>, Cu<2+>, Ni<2+>, Pb<2+>, Sr<2+>, Zn<2+> and the like, and the compound has no obvious change after the addition of other ions. The invention provides a reagent and a method which can rapidly test water solubility of the mercury ions in an environment and a biological body with high selectivity, high sensitivity and fluorescence enhancement and is applicable to the wider pH range of (3-12).

A compound comprising: (a) a drug carrier comprising coumarin-labeled-cysteine tethered mesoporous silica nanoparticles (MSNs) loaded with a pharmaceutically active agent, and (b) a fluorescein isothiocyanate-β-cyclodextrin (FITC-β-CD) covalently linked to said cysteine and blocking the release of said pharmaceutically active agent from said nanoparticles, wherein cleavage of said covalent linkage removes said cyclodextrin and releases said pharmaceutically active agent, and said coumarin and said fluorescin form a donor-acceptor pair so that said drug carrier has a first emission wavelength when the covalent linkage is intact and the cyclodextrin said present and a second emission wavelength after cleavage of said covalent linkage to remove said cyclodextrin and release said pharmaceutically active agent.

The invention discloses a phosphorescent iridium complex containing isosulfocyanate radical. The phosphorescent iridium complex has a structural formula shown as a formula (I), and is an anionic iridium complex, wherein the isosulfocyanate radical can be combined with mercury ion to generate mercuric isosulfocyanate; solvent molecule acetonitrile replaces the isosulfocyanate radical to be coordinated with iridium so as to influence the spectral quality of a generated product; and the mercury ion is detected by the change of the spectral quality, and is not interfered by other metallic ions. The complex can be used for preparing a phosphorescent stoichiometric agent for qualitatively detecting the mercury ion. The invention also provides a preparation method for the phosphorescent iridium complex containing the isosulfocyanate radical.

The invention belongs to the field of medicine materials, and particularly relates to a coordination copolymer nanometer material as well a preparation method and application thereof. The coordination copolymer nanometer material is characterized in that 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles are taken as a core, silicon dioxide is taken as a shell, and the surface of the shell is modified by a functional material. The preparation method comprises the following steps of: preparing the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles by a direct precipitation method, then coating the silicon dioxide on the surface of the magnetic nanoparticles so that the surfaces of the 1, 1'-ferrocene dicarboxylic acid-gadolinium nanoparticles carry a quantity of amidogens while the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles have water solubility, connecting to the surface of the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles through rhodamine isothiocyanate B and an amidogen effect, and finally connecting arginine glycine aspartic acid tripeptide through the amidogens. The coordination copolymer nanometer material is uniform in particle size and has good dispersity, a very good magnetic resonance imaging effect of T1 and T2, luminescent property and a target function; the synthetic process is simple; raw materials are easily available; and environmental pollution is avoided.

The invention provides a preparation method of a magnetic particle with surface modified with an isothiocyanato active group and a special application mode thereof. The preparation method comprises the following steps of: dispersing processed nano and micron grade solid-phase particles with surface modified with amino or sulfydryl active groups into an organic solvent; and mixing the particles with phenylene diisothio cyanate (PDC) for reaction to obtain a functional magnetic complex particle with surface modified with an isothiocyanato (-N=C=S) active group as a functional group. The prepared magnetic particle with surface modified with the isothiocyanato active group realizes that the common functional magnetic particle is rapidly coupled into a biomolecule and avoids toxic and side effects by adopting glutaric dialdehyde as a cross-linking agent in routine operation.

A theme of the present invention is to provide a new transition metal complex, useful as a photosensitizer dye for a photoelectric conversion element having an excellent durability and a high photoelectric conversion characteristic. The present invention provides a bivalent transition metal complex constituted of (i) a bipyridyl polyacidic ligand, such as a dicarboxybipyridyl (dcbpy) ligand, as an adsorption site for adsorption onto titanium dioxide particle surfaces; (ii) a ligand, selected from the group consisting of an isothiocyanato group, an isocyanato group, and an isoselenocyanato group and enabling absorption and excitation at long wavelengths and charge transfer; and (iii) a bipyridyl (bpy) ligand, having an alkyl group or an alkoxy group, preferably a long-chain alkyl group, and a conjugately bonded thienylvinylene group, aminophenylenevinylene group, et., and improving an absorbance of a transition metal complex and imparting stability against nucleophile to a sensitizer dye that improves an absorption bathochromic effect.

The invention discloses gardenia polysaccharide and an extraction method thereof. The gardenia polysaccharide is prepared from the following raw materials (by mass): 41 to 56 percent of total sugar, 34 to 45 percent of uronic acid and 7 to 10 percent of protein. The preparation method of the gardenia polysaccharide comprises the following steps: crushing a gardenia medicinal material, degreasing,adding water, extracting, precipitating with ethanol, repeatedly freeze-thawing to remove protein, dialyzing, and freeze-drying. The gardenia polysaccharide prepared by the method can improve cholestatic liver injury induced by alpha-naphthalene isothiocyanate (ANIT) and 3, 5-diethoxycarbonyl-1, 4-dihydro-2, 4, 6-trimethylpyridine (DDC), and reduce fibrosis induced by carbon tetrachloride (CCL4).And the liver protection effect of the gardenia polysaccharide is not reported in literatures, which indicates that the gardenia polysaccharide has obvious application prospects in the preparation ofliver protection drugs, especially in the preparation of anti-cholestatic liver injury and anti-hepatic fibrosis drugs.

The invention prepares a thiourea compound. The thiourea compound is prepared by the following steps: dissolving a substrate 5-isothiocyanate methyl isoquinoline into anhydrous dichloromethane or anhydrous tetrahydrofuran, adding different aromatic ring substituted thiazole compounds which is equivalent to 1.1 times the weight of the substrate and diisopropylethylamine which is equivalent to 3-5 times the weight of the substrate, stirring at the room temperature for 3-5 hours; after TLC detection reaction is finished, adding ethyl acetate for dilution, washing twice with 5% diluted hydrochloric acid, washing twice with saturated sodium carbonate, washing twice with water, and washing with saturated table salt water; and drying an organic phase with anhydrous sodium sulfate, carrying out reduced pressure concentration, separating through silica gel column chromatography, so as to obtain the target product. The compound has application values in antibacterial aspects and particularly has better antibacterial effects on inhibiting gram-positive bacteria such as staphylococcus aureus than a known antibacterial drug, namely penbritin, so that the thiourea compound can be taken as a novel antibacterial drug to be researched and utilized.

Belonging to marine chemical engineering technology, the invention in particular relates to a chitosan oligosaccharide derivative containing a thiourea and diethoxyphosphamide structure and a preparation method thereof. The chitosan oligosaccharide derivative containing the thiourea and diethoxyphosphamide structure is shown as formula I, wherein n=2-20. The preparation method provided by the invention includes: firstly, reacting chitosan oligosaccharide with 1, 2-dibromoethane to generate a bromoethyl chitosan oligosaccharide derivative, then adding ammonium thiocyanate for reaction with bromoethyl to generate an ethyl isothiocyanate chitosan oligosaccharide derivative, further reacting the isothiocyanate group with hydrazine hydrate to obtain an aminothioureylene ethyl chitosan oligosaccharide derivative containing a thiourea group, and finally carrying out reaction with diethyl chlorophosphate so as to obtain the chitosan oligosaccharide derivative containing the thiourea and diethoxyphosphamide structure. Infrared spectroscopic analysis determines the structure of the derivative, chitosan oligosaccharide and the introduced group effectively combine to form the chitosan oligosaccharide derivative containing the thiourea and diethoxyphosphamide structure, and the biological activity of chitosan oligosaccharide is improved.

The invention discloses an application of linear polythiourea in preparation of anti-tumor medicines and anti-virus or anti-bacterial medicines. The linear polythiourea has the structural formula represented as the formula (I), wherein R1 and R2 are independently selected from hydrogen, C1-C10 alkyl groups and aryl groups, or a hetero atom group containing N, O and S. The number-average molecular weight of the linear polythiourea is 50-50000. The invention also discloses an application of hyperbranched polythiourea in preparation of anti-tumor medicines and anti-virus or anti-bacterial medicines. The hyperbranched polythiourea is prepared by dissolving a monomer containing at least two isothiocyanic acid groups or dissolving thiourea with a monomer containing at least two amine groups in an organic solvent, performing a heating condensation polymerization reaction under protection of an inert gas, and purifying a reaction product to prepare the hyperbranched polythiourea.

The invention discloses an (S)-1-(1-phenethyl) thiourea-modified Cr-Anderson heteropolyacid catalyst, and a preparation method and an application thereof. The method comprises the following steps: firstly, reacting ammonium molybdate and chromic nitrate to generate Cr-Anderson heteropolyacid (NH4)3[Cr(OH)6Mo6O18]; carrying out hydrothermal reaction on the Cr-Anderson heteropolyacid and tris(hydroxymethyl)aminomethane in a hydrothermal kettle to obtain an organic unilateral amino-modified polyoxometallate; and with (S)-(+)-1-phenylethylamine as a raw material, synthesizing (R)-1-(1-phenethyl) isothiocyanic acid; and finally reacting (R)-1-(1-phenethyl) isothiocyanic acid and the organic unilateral amino-modified polyoxometallate to obtain the target heteropolyacid catalyst. The preparation method is simple; and the obtained heteropolyacid catalyst is applied to asymmetric dihydroxylation of olefin, is friendly to environment, can be recycled, has high enantioselectivity and high catalytic activity and is suitable for industrial production.