New preparation method of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane

Abstract

The invention discloses a new preparation method of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane, and particularly relates to a method for the extraction of sulforaphane from radish seeds or broccoli seeds or rape seeds. According to the method, air-dried radish seeds or broccoli seeds or rape seeds are used as a raw material for preparation of a radish seed crushing liquid, an enzyme deactivated broccoli seed or radish seed dispersion liquid, a degreased fine grinding liquid, a fermentation liquid, a protein removal fermentation liquid, a concentrated liquid, dry polysaccharide powder and high purity sulforaphane freeze-dried powder for preparation of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane with the purity of more than 98%. The production rate of the sulforaphane prepared by the method is high, the operation is simple, and the product has the characteristics of being high in product purity of more than 98%, green, non-pollution and the like. The product prepared by the method can be widely used in medicine and health care industry, and has cancer preventing and resistant function.

Description

1. Technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for extracting 1-isothiocyanate-4-methylsulfonyl-(2-ene)butane from radish seeds, broccoli or rape seeds. 2. Background technology [0002] Extensive research in epidemiology and pharmacology has shown that where the incidence of cancer is low, the proportion of vegetables and fruits in people's diet is relatively large, because there are active ingredients in these vegetables and fruits that can prevent or slow down the development of cancer, such as cruciferous Sulforaphane and indoles in vegetables, soybean isoflavones in soybeans, etc. have all been proven to have good anti-cancer and anti-cancer effects. [0003] Modern studies have shown that radish seeds contain a large amount of volatile oils, fats, polysaccharides, proteins, alkaloids, glucosinolates and glucosinosidases. Broccoli, also known as broccoli and broccoli, is a variety of Brassic...

AI Technical Summary

Problems solved by technology

The main problems in this method are: 1. The seeds are not degreased. Due to the blocking of oil, the sinaprin and glucuronidase in the broccoli seeds are difficult to effectively contact, resulting in a low production rate of sulforaphane; 2. Immobilization of the extracted myrosinase, the free enzyme has a short half-life and is easily inactivated; 3. The organic solvent used - acetone has a strong ability to denature the enzyme protein, resulting in a large amount of denaturation and inactivation of the myrosinase; 4 .Vacuum drying at a higher temperature, sulforaphane is unstable, easy to rearrange to form a variety of isomers, and the product purity is low

The main problems of this method are: ① supercritical CO 2 The degreasing effect is poor, only 1 / 3 of the oil in the radish seeds can be removed, and most of the oil remains in the defatted radish seed meal, hindering the release of glucosinolates and glucosinosidases, affecting the hydrolysis efficiency and product quality; and supercritical CO 2 The equipment investment is large, the device is complicated, and the operation is difficult; ②CO 2 It is difficult to fully recover the entraining agent and methanol-water eluent, etc., and the production cost is high; only the radish seeds are crushed, and the glucosinolates and glucosinosidases present in the cells are not recovered. Fully released, during the hydrolysis reaction, the diffusion rate is slow, and the amount of diffusion is limited, resulting in an increase in the hydrolysis reaction time, and a relative hydrolysis efficiency is reduced; ③The glucosinolate content in radish seeds is less, and the No added glucosinolate, so the yield of sulforaphane obtained by hydrolysis is low