2 results about "Myrosinase" patented technology

The application discloses a water extract of broccoli seeds for improving bioavailability of sulforaphane and a preparation process thereof. 2+ The application introduces phytic acid and zinc gluconate to construct a synergistic regulation system, uses the directional chelation of phytic acid to effectively deprive the essential cofactor of the ring sulfur designated protein, fundamentally blocks the generation path of the inactive nitrile, and uses the allosteric activation effect of zinc ions and the pH stability of gluconate to significantly enhance the catalytic efficiency of myrosinase.

This invention discloses a promoter-terminator combination for *Kluyveromyces martensii*, wherein the promoter... P STF2 The nucleotides are as shown in SEQ ID NO.1, and the terminator is... T STF2 The nucleotide sequence is shown in SEQ ID NO.2. The application of the *Kluyveromyces martensii* promoter-terminator combination in driving exogenous protein expression. This invention uses transcriptome sequencing technology to screen highly expressed genes from *Kluyveromyces martensii* transcriptome sequencing data using FPKM value as an indicator, obtaining an endogenous, highly efficient promoter-terminator combination. P STF2 ‑T STF2 .through egfp The reporter gene was validated and found to have stronger transcriptional activity, making it an ideal combination of regulatory elements for the efficient expression of exogenous proteins in *Kluyveromyces martensii*. Its application to the exogenous expression of myrosinase and xylanase resulted in efficient expression of both enzymes, demonstrating the good versatility and application value of this regulatory element combination.