24 results about "Myrosinase" patented technology

The present invention discloses a myrosinase-producing Cupria virescens strain and its application in preventing and controlling soil-borne diseases. Cupriavidus sp.) is deposited under CGMCC No. 34736. Further provided are uses of the strain for preventing and controlling soil-borne fungal diseases and methods for soil disinfection. The soil disinfection method provided by the present invention significantly increases the amount of isothiocyanates produced during biological fumigation technology and significantly inhibits soil-borne pathogens.

The present invention relates to a nutritional targeting composition which comprises a component 1 providing glucoraphanin, a component 2 providing myrosinase and magnesium stearate and which efficiently releases sulforaphane to activate NRF2 targets. The present invention also relates to the use of the composition in the preparation of products for preventing or treating diseases or conditions which can be prevented or treated by using sulforaphane.

The present invention is directed to methods and compositions for breeding plants with an altered flavor or odor profile. Particularly, next generation plant breeding technologies are utilized for producing plants with target genes edited to reduce pungency in flavor or odor.

The invention discloses a method for increasing the content of sulforaphane in broccoli through magnetic field assisted blanching, and belongs to the technical field of food processing. The method for improving the content of sulforaphane in broccoli through magnetic field assisted blanching comprises the following steps: (1) preparing raw materials; (2) magnetic field assisted blanching pretreatment; (3) cooling and structure keeping treatment; (4) carrying out enzymatic incubation based on a magnetic field-assisted blanching regulation state; and (5) obtaining the broccoli product with high sulforaphane content. Exogenous enzymes, chemical reagents or additives are not introduced in the process, and the activity of endogenous myrosinase and the catalytic hydrolysis path of the endogenous myrosinase are regulated by synchronously applying a physical regulation means in the blanching pretreatment process, so that the generation level of sulforaphane in a treated sample is improved. The method is suitable for a food processing system, has the advantages of mild process conditions, strong controllability, high safety and easiness in industrial amplification, and has a wide application prospect in the field of food processing.

The present invention relates to a nutritional targeting composition which comprises a component 1 providing glucoraphanin, a component 2 providing myrosinase and magnesium stearate and which efficiently releases sulforaphane to activate NRF2 targets. The present invention also relates to the use of the composition in the preparation of products for preventing or treating diseases or conditions which can be prevented or treated by using sulforaphane.

A process to remove the glucosinolates of oilseed meals, such as Brassica carinata oilseed meals, is provided. In one embodiment, exogenous myrosinase is used to convert the glucosinolates to volatile isothiocyanate compounds, which can then be removed under conditions of mild heat and negative pressure. In another embodiment, heat and pressure are used to remove glucosinolates from Brassica carinata oilseed. The processed meals may contain less than 80% of their starting levels of glucosinolates and may be suitable for use in various applications, including as animal feeds.

The application discloses a method for improving conversion rate of allyl isothiocyanate through enzymatic reaction, and the method comprises the following steps: firstly, extracting black mustard enzyme from broccoli seeds through ultrasonic extraction; secondly, flocculating the black mustard enzyme, alkyl glycoside and casein phosphopeptide under the action of sodium polyacrylate to form flocculation liquid, mixing the flocculation liquid with magnetic starch milk, and post-treating to obtain immobilized black mustard enzyme; and then, adding the immobilized black mustard enzyme into black mustard glycoside aqueous solution, and performing enzymolysis reaction under the condition of high-pressure pulse electric field to obtain allyl isothiocyanate. The application improves the conversion rate of allyl isothiocyanate through enzymatic reaction, and the product has high quality and wide application prospect.

The invention relates to a glucoraphanin and endogenous myrosinase synergistic extraction method and an extraction product, and relates to the technical field of extraction of active ingredients in plants, broccoli seeds and an extraction solution containing an inhibitor are mixed, then high-temperature instantaneous extraction is conducted, an extraction feed liquid is obtained, and the inhibitor contains tea polyphenol and menthol with the mass ratio being (2-3): 1; filtering the extracted material liquid, and performing ultrafiltration on filtrate obtained by filtering to obtain permeate liquid and trapped liquid; the permeate is subjected to instantaneous sterilization treatment, concentration and spray drying, a component containing glucoraphanin is obtained, the trapped fluid is subjected to RO membrane concentration and freeze drying, and a component containing endogenous myrosinase is obtained. According to the extraction method disclosed by the invention, the enzyme and the glucoraphanin can be extracted at the same time under the condition that the enzyme activity is inhibited, and the obtained product is rich in glucoraphanin and also contains myrosinase with certain activity.

The present invention is directed to a dried plant extract mix comprising (i) a dried extract component comprising mustard oil glycosides (glucosinolates), and (ii) a dried extract component of active myrosinase enzymes, or isolated active myrosinase enzymes, wherein the extract component (i) comprising mustard oil glycosides does not comprise active myrosinase enzymes. The invention further relates to methods for preparing the dried extracts, medical compositions comprising the dried extracts and medical uses thereof.

The current invention relates to a method of quantitatively determining the concentration of active myrosinase in a sample, the method comprising the steps of (a) adding a portion of the sample comprising active myrosinase to a first analyte assay formulation for a first period of time to generate an analyte sample, (b) adding a portion of the sample comprising inactivated myrosinase to a second analyte assay formulation for a second period of time to generate a analyte reference sample, and (c) determining the concentration of the active myrosinase in the sample. In an embodiment, the first anayte assasy formulation comprising a near infra-red absorbing and photothermally-responsive compound. The invention also relates to a formulation for use in a method of quantitatively determining the concentration of active myrosinase in a sample, and a kit of parts suitable to provide a formulation as aforementioned.

The present invention relates to a nutrition targeting composition comprising a component 1 providing sulforaphane glucosinolate, a component 2 providing myrosinase and magnesium stearate for efficient release of sulforaphane activating the NRF2 target, and the use of said composition for the preparation of a product for the prevention or treatment of a disease or condition that can be prevented or treated with sulforaphane.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an isothiocyanate bionic preparation and a preparation method thereof. The isothiocyanate bionic preparation provided by the invention comprises myrosinase microparticles and a glucosinolate extract layer coating the outer layers of the myrosinase microparticles, the myrosinase particles comprise the following components: myrosinase extract, an adhesive, a first filler and a first lubricant; the glucosinolate extract layer comprises the following components: a glucosinolate extract, a second filling agent, a disintegrating agent and a second lubricating agent. According to the isothiocyanate bionic preparation, myrosinase and glucosinolate are physically separated, long-term stable storage can be achieved under the room-temperature dry condition, when the isothiocyanate bionic preparation is orally taken and enters the gastrointestinal tract, the isothiocyanate with the enough dosage can be stably and slowly released, and the strong irritation of the isothiocyanate to the gastrointestinal tract is effectively avoided.

Methods that use a tobacco product to reduce or mitigate some of the negative effects of tobacco consumption, particularly cancer development are described. The tobacco product contains (a) nicotine, (b) at least one of an isothiocyanate or isothiocyanate precursor, (c) myrosinase and (d) a synthetic isothiocyanate. The product can further include at least one of thymoquinone, resveratrol, synthetic vitamin C, thymoquinone resveratrol and a synthetic vitamin C.

The invention relates to a nutrition targeting composition which comprises a component 1 for providing glucoraphanin, a component 2 for providing myrosinase, vitamin C and magnesium stearate and is capable of efficiently releasing sulforaphane and activating an NRF2 target spot. The invention also relates to the use of said composition for the preparation of a product for the prevention or treatment of diseases or conditions that can be prevented or treated with sulforaphane.

The invention belongs to the technical field of analysis and detection, and particularly relates to a BODIPY fluorescent probe for rapidly detecting myrosinase as well as a preparation method and application of the BODIPY fluorescent probe. When the fluorescent probe (formula I) provided by the invention is used for detecting the myrosinase, the fluorescent probe enters an active cavity of the myrosinase and then is subjected to enzymatic reaction to generate a ring closing product, so that the photo-induced electron transfer (PET) effect between aryl and fluorophore is inhibited, a fluorescent signal is recovered, and a lightening detection effect from nothing to the existence is realized. The fluorescent probe is not only suitable for quantitative detection and analysis of in-vitro myrosinase, but also can be used for in-situ and nondestructive detection and fluorescence imaging of endogenous myrosinase in plant living bodies and microorganism samples. The method has the advantages of rapidness, visualization, high sensitivity, high throughput screening and the like in the aspect of myrosinase detection, and effectively overcomes the technical limitations of complex sample pretreatment, destructiveness, difficulty in real-time and rapid observation and the like in a traditional detection method. Formula I.

The application discloses a water extract of broccoli seeds for improving bioavailability of sulforaphane and a preparation process thereof. 2+ The application introduces phytic acid and zinc gluconate to construct a synergistic regulation system, uses the directional chelation of phytic acid to effectively deprive the essential cofactor of the ring sulfur designated protein, fundamentally blocks the generation path of the inactive nitrile, and uses the allosteric activation effect of zinc ions and the pH stability of gluconate to significantly enhance the catalytic efficiency of myrosinase.

A sulforaphane supplement in gummy form, including an active ingredient composition comprising sulforaphane, glucoraphanin, and myrosinase. The supplement including a matrix structure in which sulforaphane, glucoraphanin, and myrosinase are disposed in pores and immobilized therein.

This invention discloses a promoter-terminator combination for *Kluyveromyces martensii*, wherein the promoter... P STF2 The nucleotides are as shown in SEQ ID NO.1, and the terminator is... T STF2 The nucleotide sequence is shown in SEQ ID NO.2. The application of the *Kluyveromyces martensii* promoter-terminator combination in driving exogenous protein expression. This invention uses transcriptome sequencing technology to screen highly expressed genes from *Kluyveromyces martensii* transcriptome sequencing data using FPKM value as an indicator, obtaining an endogenous, highly efficient promoter-terminator combination. P STF2 ‑T STF2 .through egfp The reporter gene was validated and found to have stronger transcriptional activity, making it an ideal combination of regulatory elements for the efficient expression of exogenous proteins in *Kluyveromyces martensii*. Its application to the exogenous expression of myrosinase and xylanase resulted in efficient expression of both enzymes, demonstrating the good versatility and application value of this regulatory element combination.

A skincare composition includes a substance having a predetermined amount of one or more cruciferous vegetables, the predetermined amount of the one or more cruciferous vegetables including stabilized glucoraphanin and active myrosinase in a stable state; exposing the substance to a liquid activator causes a chemical reaction between the stabilized glucoraphanin and the active myrosinase to create sulforaphane in an active product; and applying the activated product to a skin surface allows the sulforaphane to act upon the skin surface.

The invention discloses pickled beetle rich in glucoraphanin and sulforaphane and a preparation method of the pickled beetle, and belongs to the technical field of food processing. The preparation method comprises the following steps: blanching the beetles, carrying out freeze thawing ultrasonic treatment on the beetles, adding ascorbic acid into fermentation liquor to activate myrosinase, and finally inoculating lactic acid bacteria to ferment the beetles, so that the contents of glucoraphanin and sulforaphane in the beetles are increased; meanwhile, compared with fresh beta, the storage time of the pickled beta is prolonged, the flavor of the beta is improved, and the pickled beta is rich in bioactive substances such as glucoraphanin and sulforaphane and rich in nutrition.

The invention relates to a stable release nutritional targeting composition comprising glucoraphanin, myrosinase and a calcium salt, which can smoothly release sulforaphane. The invention also relates to a method for converting glucoraphanin into sulforaphane in vitro comprising steps of providing the composition and mixing the composition with water or an aqueous solution.

The claimed invention provides therapeutic as well as protective measures against colorectal cancer by utilizing genetically modified gut bacteria (101) in conjunction with an optimized diet high in cruciferous vegetable intake. According to the claimed system and method, engineered gut bacteria maximize the therapeutic value of the cruciferous vegetable diet to eradicate, offset or prophylactically prevent the onset of colorectal cancer. In the claimed invention gut bacteria Escherichia coli bind specifically to the heparan sulphate proteoglycan on colorectal cancer cells to secrete the enzyme myrosinase to transform host-ingested glucosinolates which are natural components of cruciferous vegetables to sulphoraphane, an organic small molecule with known anti-cancer activity.

The invention relates to a rapeseed protein concentrate efficient detoxification production method based on ethanol washing and physical passivation, and belongs to the technical field of agricultural product processing. In order to solve the problems of incomplete detoxification and large loss of nutritional ingredients in the prior art, the invention provides a collaborative detoxification method which comprises the following steps: firstly, cleaning and flaking rapeseeds, then carrying out key physical passivation treatment, instantaneously passivating myrosinase at 110-125 DEG C, and preventing glucosinolate enzymolysis; then, ethanol is adopted to leach and extract oil, the wet meal is subjected to multistage counter-current extraction and washing with a 50-70% ethanol aqueous solution, and anti-nutritional factors such as thioglycoside, phytic acid and tannin are deeply removed at 70-75 DEG C; and finally, carrying out solid-liquid separation, secondary drying and desolventizing treatment to obtain a finished product. The method realizes detoxification in the whole process from source blocking to body removal, has high detoxification efficiency, can maximally retain protein nutrition, is safe and environment-friendly in process, and is suitable for large-scale production.

The application provides a method for improving sulforaphane content in fresh-cut broccoli by combining salicylic acid and ultrasonic treatment, and belongs to the technical field of food processing and postharvest preservation. The method unifies moderate fresh-cut injury, salicylic acid induction and ultrasonic cavitation treatment into a continuous process scheme, avoids quality deterioration caused by excessive cutting, promotes accumulation of glucoraphanin and improves contact efficiency of glucoraphanin and endogenous myrosinase, so that the generation level of sulforaphane is improved. The method is simple in steps, short in treatment time, controllable in cost, and suitable for processing of high-sulforaphane fresh-cut broccoli products.